GRM 2013: Harnessing the potential of MAGIC population for gene discovery and breeding applications in chickpea  -- P Gaur
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GRM 2013: Harnessing the potential of MAGIC population for gene discovery and breeding applications in chickpea -- P Gaur

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GRM 2013: Harnessing the potential of MAGIC population for gene discovery and breeding applications in chickpea -- P Gaur Presentation Transcript

  • 1. Research Initiative Perspectives Chickpea Pooran Gaur GCP-GRM 29 Sep 2013, Lisbon, Portugal
  • 2. Target countries for chickpea RI India Ethiopia Kenya Tanzania
  • 3. Projects under Chickpea RI • G6010.04: TL-I, Phase 2, Objective 4: Improve chickpea productivity for marginal environments in sub-Saharan Africa and Asia (2010-14) • TL-I complementary project in India - funded by The Department of Biotechnology, Govt. of India (2011-14) • G4011.08: Harnessing the potential of MAGIC populations for gene discovery and breeding applications in chickpeas (2011-2014) Indian Agricultural Indian Institute Ethiopean Institute Egerton University Research Institute Pulses Research of Agril. Research (EU), Kenya (IARI) (IIPR) (EIAR)
  • 4. Larry’s guidelines for this presentation 1. Indicate how many projects were discussed 2. Indicate how many of these will not be completed by the end of 2014 3. What activities and products will not be completed 4. In general terms what is the plan to finish the activity and the product(s) 5. Even if finished, indicate specific activities (if any) you feel should be continued, if funding could be found. 6. Potential sources of new funding, very general strategy for obtaining this. 7. Results of your product catalogue review
  • 5. Ethiopia • Evaluation of 309 F4 progenies of ICCV 4958 x Ejere • Selection of promising plants from F3 populations of 4 crosses Kenya • Evaluation of 71 F5 progenies from 9 crosses and 341 progenies from 12 crosses (ICRISAT- Nairobi) • Advancement of 88 F4 progenies from 3 crosses and 39 F3 progenies from 2 crosses (EU-Njoro) India • Evaluation of 3 advanced breeding lines in a station trial along with other breeding lines • Evaluation of 120 F5/F6 progenies from 9 crosses Development and evaluation of pre-breeding populations Activities in progress……
  • 6. Evaluation of MABC lines Replicated yield trials on MABC lines during 2013/14 Locations Trial 1: 22 BC3F5 lines from Set-2 of (ICCV 93954 x ICC 4958) x 3*ICCV 93954 Ethiopia (1), Tanzania (1) Kenya (2) Trial 2: 10 BC3F5 lines – 8 from ICCV 92318 x ICC 8261) x 3*ICCV 92318 and 2 from (ICCV 92311 x ICC 8261) x 3*ICCV 92311 Ethiopia (1), Tanzania (1) Kenya (2) Trial 3: 25 BC3F5 lines from (ICCV 10 x ICC 4958) x 3*ICCV 10 Ethiopia (1), Kenya (2), India (4) Trial 4: 13 BC3F5 lines selected from Set I of (ICCV 93954 x ICC 4958) x 3*ICCV 93954 Ethiopia (2) Trial 4: 10 BC3F5 lines selected from Set I of (ICCV 93954 x ICC 4958) x 3*ICCV 93954 Kenya (1) Trial 5: 20 BC3F5 lines selected from Set I and Set II of (ICCV 93954 x ICC 4958) x 3*ICCV 93954 India (4) Trial 6: 16 NILs (BC6F4) from (ICCV 92311 x ICC 8261) x 6*ICCV 92311 and 7 NILs from (ICCV 92318 x ICC 8261) x 6*ICCV 92318 India (4)
  • 7. MABC in progress Cross Plan for 2013/14 Location Ejere × ICC 4958 BC3F3 Seed multiplication EIAR, Ethiopia ICCV 97105 × ICC 4958 BC4F2 Selection of homozygous plants & seed multiplication EU, Kenya ICCV 95423 × ICC 4958 BC3F3 Seed multiplication EU, Kenya DCP92-3 × ICC 4958* Generate BC2F1 IIPR, India KWR108 × ICC 4958* BC2F2 Selection of homozygous plants IIPR, India Pusa 362 × ICC 4958* Generate BC3F1 IARI, India *Under DBT-supported Indian project
  • 8. MARS Cross Plan for 2013/14 Location JG 130 x ICCV 05107 8 lines selected; Two-way crosses completed; 4- way & 8-way crosses will be made EU-Kenya and ICRISAT- Patancheru JG 11 × ICCV 04112* 8 lines selected; Two-way crosses completed; 4- way & 8-way crosses will be made ICRISAT-Patancheru Pusa 372 x JG 130* Genotyping of F3 and seed multiplication IARI-New Delhi DCP 92-3 x ICCV 10* Genotyping of F3 and seed multiplication IIPR-Kanpur *Under DBT-supported Indian project
  • 9. MAGIC lines To be completed by 2014: • Preliminary evaluation of 1200 MAGIC lines in augmented design under rainfed and irrigated conditions. • Genotyping of 1200 MAGIC lines  Planned by at least 1500 SNP markers (funds available)  Desired to do whole genome re-sequencing (additional funds required) • Selection of MAGIC lines based on genome-wide haplotypes To be completed after 2014 • Phenotyping of selected MAGIC lines for drought tolerance related traits in India, Ethiopia and Kenya (Additional funds required) • Genome-wide association study (GWAS) for drought tolerance related traits using genome-wide haplotype and phenotyping data
  • 10. Plans to continue the activities and potential sources of funding • Ongoing breeding programs of ICRISAT and NARS partners • Possible next phase of Tropical Legumes Project (TL-III) • CGIAR Research Program on Grain Legumes • Any additional generous support from GCP
  • 11. Review of Product Catalogues (A) Genomic Resources – already in public domain • A microsatellite kit for the chickpea reference germplasm set • DArT marker array for chickpea • A validated chickpea germplasm reference set • A SNP dataset for chickpea • SSR markers for chickpea • A reference genetic map for chickpea • Drought and salinity responsive ESTs in chickpea • Markers linked to a genomic region (QTL-hotspot) controlling several drought tolerance traits in chickpea • A transcriptome assembly and SNP dataset for chickpea • Integrated QTL and physical maps of chickpea • Draft genome sequence of chickpea and re-sequencing data on 90 genotypes
  • 12. Review of Product Catalogues (B) Germplasm Resources (most of these will be maintained at ICRISAT, but some by partners) Already available • Reference germplasm set* • RIL mapping population for drought tolerance traits* • MAGIC lines • QTL-hotspot introgression lines (several to be added later) To be available after 2014 • Improved breeding lines developed from pre-breeding populations • Improved breeding lines developed using MARS *added to the product catalogue
  • 13. Chickpea Community of Practice
  • 14. Acknowledgements • Generation Challenge Program (GCP) • Bill & Melinda Gates Foundation (BMGF)
  • 15. ICRISAT is a member of the CGIAR Consortium Thank you!