Electrophoresis

3. INTRODUCTION:-
Electrophoresis is a physical method of analysis which
involves separation of the compounds that are capable of
acquiring electric charge in conducting electrodes
Ions that are suspended between two electrodes tends to
travel towards the electrodes that bears opposite charges.

6. TYPES OF ELECTROPHORESIS
1) Zone Electrophoresis
a) Gel Electrophoresis
b) Paper Electrophoresis
c) Thin Layer Electrophoresis
d) Cellulose acetate Electrophoresis
2) Moving Boundary Electrophoresis
a) Capillary Electrophoresis
b) Isotachophoresis
c) Isoelectric Focussing
d) Immuno Electrophoresis

7. Gel Electrophoresis:
SDS-PAGE Gel Electrophoresis
Agarose Gel Electrophoresis

8. ElectrophoresisEquipment

10. Preparation Method :-

11. Mechanism of Electrophoresis:-

12. PAPER ELECTROPHORESIS
Horizontal paper Electrophoresis
Vertical paper Electrophoresis

13. Factors Affecting Electrophoresis

14. Clinical applications of Electrophoresis
 Serum Protein Electrophoresis
 Lipoprotein Analysis
 Diagnosis of Haemoglobinopathies and Haemoglobin
 Determination of Serum Protein Phenotypes
 Genotyping of Proteins
 Small Molecules (Drugs, Steroids) Monitoring
 Cerebrospinal Fluid Analysis
 Urine Analysis ( determination of GNs)