Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Anti-Histone H3 (Phospho-Tyr41) - http://www.stjohnslabs.com/histone-h3-phospho-tyr41-antibody?filter_name=STJ97138 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

1. Result
“The antibody is showing specific 17 kd band with other nonspecific bands in whole cell lysates. Figure. Western Blot
Analysis of Phospho-H3-Tyr 41 in different Lymphoma cell lines ” - G. P. Maiti, External Laboratory.
Cell Line:
Lymphoma cell lines whole cell
lysate
Method of validation: Western Blot
Primary Antibody:
ST97138 Anti-Histone H3
(Phospho-Tyr41) Antibody
Secondary Antibody Sheep Anti Rabit
Dilution ratio: 1:1000
Antibody Customer Review:
ST97138 Anti-Histone H3 (Phospho-Tyr41)
Antibody Specificity:
Antibody Rating:
Protocol
Treatment of materials: None;
E.g. Gel electrophoresis: 7.5% polyacrylamide gel, constant voltage 20 V, overnight;
Transfer: 0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant voltage 20 V overnightl
Blocking: 1X TBST with 5% BSA for 2 hr.
Primary antibody probing: primary antibody was added to the solution of 1X TBST with 5% skimmed milk powder, 4˚C
for overnight.
Membrane wash: 1X TBST wash for 3 times.
Secondary antibody probing: secondary antibody was added to the solution of 1X TBST with 5% skimmed milk powder
with 1:5000 dilution ratio, for 1hr at room temperature.
Membrane wash: 1X TBST wash for 3 times.
Visualization: ECL visualization for 5 minutes.
Gel electrophoresis information：12% polyacrylamide gel, constant voltage 100 V, 60 min.
Transfer information：0.45 nm PVDF membrane, 1 piece of gel (semi-dry transfer), constant voltage 110mA, overnight.
Lane No. Antigen
Loading
amount
Primary anti-
body
Primary
antibody
dilution
ratio
Secondary
antibody dilution
ratio
Target band KD
Visualization
time
1 DHL6 20ug
ST97138 Anti-
Histone H3
(Phospho-
Tyr41) Anti-
body
1:1000 2:5000 ~17KD 5min