The document summarizes the qualifications and work experience of James Horstman. It outlines his 2+ years of experience in plant DNA extraction, quantification and PCR amplification at the USDA. It also notes he has generated and characterized over 12 wheat transgenic lines and has experience transforming rice, Brachypodium distachyon, and Brachypodium silvaticum. Currently he works as a Laboratory Assistant at the USDA-ARS where he maintains and performs plant transformations and characterizes transgenic plants.
Targeted Breeding Applications of CRISPR-CasKate Barlow
Doane Chilcoat, Director, Applied Technology Systems, DuPont Pioneer
CRISPR-Cas as an advanced plant breeding tool is a more efficient way to improve plants and help farmers produce more and better food, with fewer resources. The superior properties of CRISPR-Cas allows DuPont Pioneer scientists to develop innovative and sustainable seed products for growers similar to those realized through conventional plant breeding, but with even greater efficiency, accuracy and quality. Pioneer is leading the application of this tool to develop customized agriculture solutions. In this talk, potential product targets of this promising technology will be discussed. Approaches to fostering social license and developing an open innovation model for CRISPR-Cas will also be reviewed.
Need to revolutionize the crop breedingamoldchokhat
Mankind is facing an enormous challenge of food insecurity. By the year 2050, the population of the world is projected to increase by 35% to a whopping 9 billion; and an almost doubling of global food production is needed to feed the planet. This additional food for humans and farm animals has to come from the existing land, through maximization of genetic potential and relatively quickly. This demand for unprecedented productivity in agriculture needs to be realized in the presence of growing challenges of climate change, shortsighted land-use practices and increasing cost of agriculture despite the rate of improved adoption of technology in crop breeding. Recent advances in our understanding of genes and genomes combined with development of novel tools in biotechnology will play a vital role in accelerating efforts in plant breeding. Genomics assisted breeding assists the breeders in precise selection to enhance the effectivity and enhancement of the precise selection to develop a new cultivars.
Targeted Breeding Applications of CRISPR-CasKate Barlow
Doane Chilcoat, Director, Applied Technology Systems, DuPont Pioneer
CRISPR-Cas as an advanced plant breeding tool is a more efficient way to improve plants and help farmers produce more and better food, with fewer resources. The superior properties of CRISPR-Cas allows DuPont Pioneer scientists to develop innovative and sustainable seed products for growers similar to those realized through conventional plant breeding, but with even greater efficiency, accuracy and quality. Pioneer is leading the application of this tool to develop customized agriculture solutions. In this talk, potential product targets of this promising technology will be discussed. Approaches to fostering social license and developing an open innovation model for CRISPR-Cas will also be reviewed.
Need to revolutionize the crop breedingamoldchokhat
Mankind is facing an enormous challenge of food insecurity. By the year 2050, the population of the world is projected to increase by 35% to a whopping 9 billion; and an almost doubling of global food production is needed to feed the planet. This additional food for humans and farm animals has to come from the existing land, through maximization of genetic potential and relatively quickly. This demand for unprecedented productivity in agriculture needs to be realized in the presence of growing challenges of climate change, shortsighted land-use practices and increasing cost of agriculture despite the rate of improved adoption of technology in crop breeding. Recent advances in our understanding of genes and genomes combined with development of novel tools in biotechnology will play a vital role in accelerating efforts in plant breeding. Genomics assisted breeding assists the breeders in precise selection to enhance the effectivity and enhancement of the precise selection to develop a new cultivars.
Creative Biogene offers the best services in the field of microbe genome editing which help to add new functions or to optimize an existing bioprocess. With a lot of talent scientists and technicians, Creative Biogene has built an excellent and mature platform to modify a series of different microbes.
https://www.creative-biogene.com/services/microbe-genome-editing-service
The use of the term cisgenesis is an attempt to distinguish GM plants or other organisms produced in this way from transgenics that is GM plants that contain DNA from unrelated organisms. Schouten et al. (2006) introduced the term cisgenesis and defined cisgenesis as the modification in the genetic background of a recipient plant by a naturally derived gene from a cross compatible species including its introns and its native promoter and terminator flanked in the normal sense orientation. Since cisgenes shared a common gene pool available for traditional breeding the final cisgenic plant should be devoid of any kind of foreign DNA viz., selection markers and vector- backbone sequences. Sometimes the word cisgenesis is also referred to as Agrobacterium-mediated gene transfer from a sexually compatible plant where only the T-DNA borders may be present in the recipient organism after transformation (EFSA, 2012). The cisgenesis precludes linkage drag, and hence, prevents hazards from unidentified hitch hiking genes (Schouten, and Jacobsen, 2008). Compared to transgenesis, one of the disadvantages shared by cisgenesis is that characters outside the sexually compatible gene pool cannot be introduced. Furthermore, development of cisgenic crops involves extraordinary proficiency and time compared to transgenic crops. Therefore, the required genes or fragments of genes may not be readily accessible but have to be isolated from the sexually compatible gene pool (Holme et al., 2013).
On 16 February 2012, European Food Safety Authority (EFSA, 2012) reported the detail study concerning the safety aspects of cisgenic plants and validated that cisgenic plants are secure to be used in terms of environment, food and feed, similar to the traditionally bred plants. However, the present GMO regulation keeps the cisgenic micro-organisms out from its supervision. The first scientific statement of bringing forth a true plant obtained by cisgenic approach was reported in apple through the insertion of the internal scab resistance gene HcrVf2 influenced by their own regulatory genes into the cultivar Gala, a scab susceptible cultivar (Vanblaere et al., 2011). Barley with improved phytase activity was produced successfully by Holme et al. 2011, through cisgenic approach. Late blight resistant potatoes have developed by cisgene stacking of R- gene (jo et al., 2014).
Incorporating Agricultural Biotechnology into the Classroombio-link
Dr. Daisy Zhang talks about having her students do GMO testing, PCR, plant genomic DNA isolation, doing plant tissue culture, and engineering plants with Agrobacterium at Del Mar College.
it cover almost all content in cis/intragesis, right from introduction definition, explanation, production of marker free transgenic, intragenic vector construction, regulatory guide lines, current and future status, limitation, advantage over existing technique, swot analysis etc
its very useful for your seminar and presentations. it contain lot of picture, table, figure for your easy understanding
thank you
Mahesh
B4FA 2012 Tanzania: Combating cassava brown streak disease - Fortunus Anton K...b4fa
Presentation at the November 2012 dialogue workshop of the Biosciences for Farming in Africa media fellowship programme in Arusha, Tanzania.
Please see www.b4fa.org for more information
Recombinant Antibody Overview II - Creative BiolabsCreative-Biolabs
It is created by Creative Biolabs who is specialized in providing recombinant antibodies and engineered antibodies. The part two of recombinant antibody overview, we will explain introduction of recombinant antibody ant recombinant antibody expression.
Development of Monoclonal Antibodies -Creative BiolabsCreative-Biolabs
In this slide, Creative Biolabs will introduce technologies of monoclonal antibody development, including hybridoma, surface display, b cell immortalization, and transgenetic technology. If you have any question about mab production, welcome to contact us at info@biolabsantibody.com
Molecular Basis for Genetic Resistance of Fusarium virguliforme, the Causal A...Chloe Siegel
This poster was presented at the Undergraduate Research Symposium at the University of Illinois at Urbana-Champaign. It summarizes a semester-long research project I participated in through the Department of Natural Resources and Environmental Sciences.
Yeast display is a novel technique widely used to express the proteins at the yeast surface after translation and maturation in a eukaryotic system. The slide named yeast display technology is created by Creative Biolabs who has established unique Ultraff™ yeast display platform to meet customers'requirement. In the slide, we will give you a comprehensive introduction to yeast display, including yeast display system, the process of yeast display system construction and application of yeast display. It is believed that you can fully understand yeast display technology after studying the slide.
Creative Biogene offers the best services in the field of microbe genome editing which help to add new functions or to optimize an existing bioprocess. With a lot of talent scientists and technicians, Creative Biogene has built an excellent and mature platform to modify a series of different microbes.
https://www.creative-biogene.com/services/microbe-genome-editing-service
The use of the term cisgenesis is an attempt to distinguish GM plants or other organisms produced in this way from transgenics that is GM plants that contain DNA from unrelated organisms. Schouten et al. (2006) introduced the term cisgenesis and defined cisgenesis as the modification in the genetic background of a recipient plant by a naturally derived gene from a cross compatible species including its introns and its native promoter and terminator flanked in the normal sense orientation. Since cisgenes shared a common gene pool available for traditional breeding the final cisgenic plant should be devoid of any kind of foreign DNA viz., selection markers and vector- backbone sequences. Sometimes the word cisgenesis is also referred to as Agrobacterium-mediated gene transfer from a sexually compatible plant where only the T-DNA borders may be present in the recipient organism after transformation (EFSA, 2012). The cisgenesis precludes linkage drag, and hence, prevents hazards from unidentified hitch hiking genes (Schouten, and Jacobsen, 2008). Compared to transgenesis, one of the disadvantages shared by cisgenesis is that characters outside the sexually compatible gene pool cannot be introduced. Furthermore, development of cisgenic crops involves extraordinary proficiency and time compared to transgenic crops. Therefore, the required genes or fragments of genes may not be readily accessible but have to be isolated from the sexually compatible gene pool (Holme et al., 2013).
On 16 February 2012, European Food Safety Authority (EFSA, 2012) reported the detail study concerning the safety aspects of cisgenic plants and validated that cisgenic plants are secure to be used in terms of environment, food and feed, similar to the traditionally bred plants. However, the present GMO regulation keeps the cisgenic micro-organisms out from its supervision. The first scientific statement of bringing forth a true plant obtained by cisgenic approach was reported in apple through the insertion of the internal scab resistance gene HcrVf2 influenced by their own regulatory genes into the cultivar Gala, a scab susceptible cultivar (Vanblaere et al., 2011). Barley with improved phytase activity was produced successfully by Holme et al. 2011, through cisgenic approach. Late blight resistant potatoes have developed by cisgene stacking of R- gene (jo et al., 2014).
Incorporating Agricultural Biotechnology into the Classroombio-link
Dr. Daisy Zhang talks about having her students do GMO testing, PCR, plant genomic DNA isolation, doing plant tissue culture, and engineering plants with Agrobacterium at Del Mar College.
it cover almost all content in cis/intragesis, right from introduction definition, explanation, production of marker free transgenic, intragenic vector construction, regulatory guide lines, current and future status, limitation, advantage over existing technique, swot analysis etc
its very useful for your seminar and presentations. it contain lot of picture, table, figure for your easy understanding
thank you
Mahesh
B4FA 2012 Tanzania: Combating cassava brown streak disease - Fortunus Anton K...b4fa
Presentation at the November 2012 dialogue workshop of the Biosciences for Farming in Africa media fellowship programme in Arusha, Tanzania.
Please see www.b4fa.org for more information
Recombinant Antibody Overview II - Creative BiolabsCreative-Biolabs
It is created by Creative Biolabs who is specialized in providing recombinant antibodies and engineered antibodies. The part two of recombinant antibody overview, we will explain introduction of recombinant antibody ant recombinant antibody expression.
Development of Monoclonal Antibodies -Creative BiolabsCreative-Biolabs
In this slide, Creative Biolabs will introduce technologies of monoclonal antibody development, including hybridoma, surface display, b cell immortalization, and transgenetic technology. If you have any question about mab production, welcome to contact us at info@biolabsantibody.com
Molecular Basis for Genetic Resistance of Fusarium virguliforme, the Causal A...Chloe Siegel
This poster was presented at the Undergraduate Research Symposium at the University of Illinois at Urbana-Champaign. It summarizes a semester-long research project I participated in through the Department of Natural Resources and Environmental Sciences.
Yeast display is a novel technique widely used to express the proteins at the yeast surface after translation and maturation in a eukaryotic system. The slide named yeast display technology is created by Creative Biolabs who has established unique Ultraff™ yeast display platform to meet customers'requirement. In the slide, we will give you a comprehensive introduction to yeast display, including yeast display system, the process of yeast display system construction and application of yeast display. It is believed that you can fully understand yeast display technology after studying the slide.
12 years of professional experience as a Data specialist and application developer. Development of statistical
analysis has heavily involved statistical packages with R, SPSS, SQL and SAS. Software applications,
web applications and webservices have required development relevant to J2EE, .NET, and Ruby on Rails
frameworks. Utilized SQL throughout my professional work and academic research. About 60% of my
database work has been with MySQL, and 20% each for both Oracle and SQL Server. Multiple years of
working within Agile development environments, and multiple years working independently on developing
statistical predictive models and optimizing processes.
ICRISAT Global Planning Meeting 2019: Research Program – Asia by Dr Pooran Ga...ICRISAT
Refining Product Concepts and ensuring alignment of Crop Breeding efforts to Product Concepts and Modernization of crop improvement programs to accelerate genetic gain.
Speed Breeding and its implications in crop improvementANILKUMARDASH2
Introduction
History of speed breeding
Methods of speed breeding
Advantages over conventional breeding
Integration with various technologies
Case studies
Opportunities and challenges
Conclusions
Solutions for Impact in Emerging Markets: The role of biotechnologyICRISAT
To develop and deploy state-of-the-art infrastructure for conduct of transgenic research and to act as a clearinghouse for technology inputs, transgenic research leads/ prototypes with proof of concept derived from Indian research institutes, universities, and other likely sources.Also to evolve the technology to a point where a practical application can be demonstrated, and transfer this “evolved” technology for product development and distribution to appropriate agencies.
How Does Farming Weeds Answer Questions About Pesticide Risk? Crop Protection...Covance
Covance was approached by a European company producing a standard herbicide for maize who had been asked by the regulator to conduct a study to provide data to refine the small mammal risk assessment. The concern was not only the residue on the maize, but also on the weeds that the herbicide targeted at the time of application.
A genetically modified organism (GMO) is any organism whose genetic material has been altered using genetic engineering techniques. The exact definition of a genetically modified organism and what constitutes genetic engineering varies, with the most common being an organism altered in a way that "does not occur naturally by mating and/or natural recombination". A wide variety of organisms have been genetically modified (GM), from animals to plants and microorganisms.
1. James David Horstman
183 Eureka St, #2 San Francisco, CA 94114
(415) 988-1295; jameshorstman@sbcglobal.net
BIOSCIENCE ACCOMPLISMENTS
2+ years of experience with plant DNA extraction, quantification and PCR
amplification in Crop Improvement and Genetics laboratory at the USDA.
Experienced working in both a biological safety hood and laminar flow hood for
plant callus maintenance and propagation.
Generated and characterized 12+ wheat transgenic lines for a Hessian Fly
project in the Thilmony Laboratory at the USDA.
Experienced in rice (Oryza sativa), Brachypodium distachyon, Brachypodium
silvaticum transformation through agrobacterium for characterization of promoter
activity.
Highly skilled in wheat (Triticum aestivum) transformation through Bio-Rad
Gene Gun.
QUALIFICATIONS
Molecular Biology: DNA extraction, PCR amplification and DNA quantification.
Operation, maintenance, calibration, and syncronization of equipment: Bio-
Rad gene gun, GC-MS, FID, tissue grinder, centrifuge, pH meter, balance,
electrophoresis apparatus, thermocycler, nanodrop, spectrophotometer, tissue
culture hood, light microscope and autoclave.
Data Management: Collecting, recording, tabulating, organizing, statistically
analyzing, and summarizing using PowerPoint presentations/Excel/Word.
Literature Research: Performing research for new procedures or techniques to
use in the laboratory; determining the kinds and frequency of experiments and
observations.
Greenhouse: Caring for plants (i.e. wheat, Brachypodium distachyon/sylvaticum,
and Alamo switchgrass), observing and recording data relating to plant studies,
inventory seed collections, and phenotyping.
General Lab Duties: Glassware sterilization, media preparation, disposal of
waste materials, plant tissue culture, sterile techniques, maintenance of a neat and
orderly laboratory and greenhouse.
Computer Skills: Adobe Photoshop, Word, Powerpoint, Excel, Firefox, Internet
Explorer, Safari, Google Chrome.
Transferable Skills: Creative, independent yet a good team player; strong
communication and problem solving skills; organized and detail oriented; great
leadership and supervising skill; fast learning ability; and good work ethic.
2. WORK EXPERIENCE
Laboratory Assistant I, United States Department of Agriculture, Agricultural
ResearchService (USDA-ARS), Albany, CA 01/2013-Present
- Maintained and performed Agrobacterium transformation of rice and
Brachypodium distachyon.
- Maintained and performed Bio-Rad Gene Gun transformation in wheat
- Characterized transgenic plants using Gus staining and Basta resistant assays.
- Maintained inventory of transgenic seed lines.
- Maintained plants in greenhouse and growth chambers.
Laboratory Assistant I, UC Davis & United States Department of Agriculture,
Agricultural ResearchService (USDA-ARS), Albany, CA 04/2015-Present
In-situ Volatile collection of Pistachio and Pomegranates – Collect volatile emissions to
determine differences at various growth points in the field .
- Collect samples from multiple sources in field to determine validity of tests done
in the laboratory
- Test for volatiles of early split stage to determine if there is correlation between
early splitting and infestation of pests and fungi.
- Compile GC-MS data from pistachio and pomegranate samples into a database.
- Utilize GC-MS to compare concentration of compounds with different flow rates
and retention times.
- Validate data analysis using compound standards.
- HOOD - Preform entomological assay to determine Navel Orange Worm’s
attraction to different tree nuts and chemical blends.
- Measure volatile output of compounds using GC-MS.
- Capture insects and determine sex of insects before starting assay.
- Count number of eggs on traps after assay to determine level of insect attraction
- Maintain hoods and insects for testing.
EDUCATION
- City College of San Francisco, Biotechnology Certificate 06/2013
AWARDS
- City College of San Francisco, Biotechnology Laboratory Assistant Scientific Poster
Award 05/2013
