1. Solexa sequencing was invented in 1997 by David Klenerman and Shankar Balasubramanian in the chemistry department at the University of Cambridge.
2. It was developed by a spin-out company in Saffron Walden and sold for £420 million in 2006.
3. Solexa sequencing enabled sequencing a human genome for around £700 in less than a day, compared to £2 billion over 14 years using previous methods.
The race to unlock one of the great mysteries of life, the structure of DNA.
Register to explore the whole course here: https://school.bighistoryproject.com/bhplive?WT.mc_id=Slideshare12202017
"Phylogeny-Driven Approaches to Genomics and Metagenomics" talk by Jonathan E...Jonathan Eisen
This document summarizes a talk given by Jonathan Eisen on October 23, 2013 at the University of Washington. The talk discussed four eras of sequencing and microbes, beginning with the establishment of the tree of life in the late 1970s/early 1980s based on rRNA sequencing. It then covered the use of rRNA sequencing in environmental samples in the 1990s to characterize microbial communities. Finally, it discussed how next generation sequencing revolutionized rRNA PCR and allowed for deeper sequencing of more samples and finer-scale spatial sampling of microbial communities.
1) The document discusses the discovery of antimatter and parity in physics. Paul Dirac discovered parity in matter in 1933 based on Einstein's equation on energy.
2) Key antimatter discoveries of the 20th century included positrons, antiprotons, and antiatoms created by pairing positrons and antiprotons.
3) A verse from the Quran references parity between what is produced from "the earth," "themselves," and "things unknown to them," which refers to the modern discoveries of parity in plants, living organisms, and matter/antimatter.
The RNA world hypothesis proposes that RNA, not DNA or proteins, was the first self-replicating molecule and the central player in early life. RNA can both store genetic information and catalyze chemical reactions, acting as both the genome and enzymes. Evidence for this includes the discovery that some RNA molecules can self-replicate and that the ribosome, the cell's protein-building machinery, has RNA as its key component. While still debated, the RNA world hypothesis provides a plausible explanation for how life could have originated and evolved prior to the development of DNA and proteins.
The document provides information about DNA discovery and the Human Genome Project. It discusses the roles of James Watson, Francis Crick, Rosalind Franklin and Maurice Wilkins in discovering the structure of DNA. It describes the Human Genome Project, which sequenced the entire human genome, and some ways its findings have been applied. It also addresses some ethical issues raised by the project. The document includes questions for students to answer about DNA, cells, and the roles of scientists in DNA discovery.
Darwins Evolution Is Technically Impossibledarwiniswrong
This document presents 10 "facts" intended to argue that Darwin's theory of evolution is technically impossible. It claims that evolution violates the first law of thermodynamics by increasing order from disorder. It argues that complex biological structures like the bombardier beetle's defense system and bacterial flagellum could not have evolved gradually. It also argues that the lack of transitional fossils, the age of the earth and moon based on dust accumulation, and the existence of irreducibly complex biological structures disprove evolution. The document concludes by questioning whether scientists are willing to face these supposed facts.
This document summarizes the history of life classification systems from 1735 to the present. It discusses the major classification systems proposed by Linnaeus, Haeckel, Whittaker, and Woese. The key points are:
- Linnaeus originally grouped organisms into two kingdoms in 1735 based on physical characteristics. Whittaker later expanded this to five kingdoms in 1969.
- In 1990, Woese proposed the current three domain system, separating organisms into Archaea, Bacteria and Eukaryota based on genetic research.
- Classification systems have evolved over time to increasingly reflect evolutionary relationships and genetic evidence, moving from morphology-based to phylogenetics-based
- There are three domains that classify life: Archaea, Bacteria, and Eukarya. Archaea and bacteria are prokaryotes that lack nuclei and organelles, while eukaryotes have nuclei and membrane-bound organelles.
- Early classification systems grouped organisms based on visible characteristics, but modern taxonomy seeks to classify organisms based on inferred evolutionary relationships and genetic relatedness using DNA evidence.
- Major systems of classification over time have included Linnaeus' two kingdoms, Whittaker's five kingdoms, and Woese's three domain system, which is currently widely accepted. Classifications continue to be refined as new genetic evidence is discovered.
The race to unlock one of the great mysteries of life, the structure of DNA.
Register to explore the whole course here: https://school.bighistoryproject.com/bhplive?WT.mc_id=Slideshare12202017
"Phylogeny-Driven Approaches to Genomics and Metagenomics" talk by Jonathan E...Jonathan Eisen
This document summarizes a talk given by Jonathan Eisen on October 23, 2013 at the University of Washington. The talk discussed four eras of sequencing and microbes, beginning with the establishment of the tree of life in the late 1970s/early 1980s based on rRNA sequencing. It then covered the use of rRNA sequencing in environmental samples in the 1990s to characterize microbial communities. Finally, it discussed how next generation sequencing revolutionized rRNA PCR and allowed for deeper sequencing of more samples and finer-scale spatial sampling of microbial communities.
1) The document discusses the discovery of antimatter and parity in physics. Paul Dirac discovered parity in matter in 1933 based on Einstein's equation on energy.
2) Key antimatter discoveries of the 20th century included positrons, antiprotons, and antiatoms created by pairing positrons and antiprotons.
3) A verse from the Quran references parity between what is produced from "the earth," "themselves," and "things unknown to them," which refers to the modern discoveries of parity in plants, living organisms, and matter/antimatter.
The RNA world hypothesis proposes that RNA, not DNA or proteins, was the first self-replicating molecule and the central player in early life. RNA can both store genetic information and catalyze chemical reactions, acting as both the genome and enzymes. Evidence for this includes the discovery that some RNA molecules can self-replicate and that the ribosome, the cell's protein-building machinery, has RNA as its key component. While still debated, the RNA world hypothesis provides a plausible explanation for how life could have originated and evolved prior to the development of DNA and proteins.
The document provides information about DNA discovery and the Human Genome Project. It discusses the roles of James Watson, Francis Crick, Rosalind Franklin and Maurice Wilkins in discovering the structure of DNA. It describes the Human Genome Project, which sequenced the entire human genome, and some ways its findings have been applied. It also addresses some ethical issues raised by the project. The document includes questions for students to answer about DNA, cells, and the roles of scientists in DNA discovery.
Darwins Evolution Is Technically Impossibledarwiniswrong
This document presents 10 "facts" intended to argue that Darwin's theory of evolution is technically impossible. It claims that evolution violates the first law of thermodynamics by increasing order from disorder. It argues that complex biological structures like the bombardier beetle's defense system and bacterial flagellum could not have evolved gradually. It also argues that the lack of transitional fossils, the age of the earth and moon based on dust accumulation, and the existence of irreducibly complex biological structures disprove evolution. The document concludes by questioning whether scientists are willing to face these supposed facts.
This document summarizes the history of life classification systems from 1735 to the present. It discusses the major classification systems proposed by Linnaeus, Haeckel, Whittaker, and Woese. The key points are:
- Linnaeus originally grouped organisms into two kingdoms in 1735 based on physical characteristics. Whittaker later expanded this to five kingdoms in 1969.
- In 1990, Woese proposed the current three domain system, separating organisms into Archaea, Bacteria and Eukaryota based on genetic research.
- Classification systems have evolved over time to increasingly reflect evolutionary relationships and genetic evidence, moving from morphology-based to phylogenetics-based
- There are three domains that classify life: Archaea, Bacteria, and Eukarya. Archaea and bacteria are prokaryotes that lack nuclei and organelles, while eukaryotes have nuclei and membrane-bound organelles.
- Early classification systems grouped organisms based on visible characteristics, but modern taxonomy seeks to classify organisms based on inferred evolutionary relationships and genetic relatedness using DNA evidence.
- Major systems of classification over time have included Linnaeus' two kingdoms, Whittaker's five kingdoms, and Woese's three domain system, which is currently widely accepted. Classifications continue to be refined as new genetic evidence is discovered.
The document discusses the history and advancements of DNA sequencing. It describes how the discovery of the DNA structure in 1953 enabled sequencing. The first human genome was sequenced from 1977-2003 using Fred Sanger's method and cost $3 billion. New single molecule detection techniques invented in 1997 dramatically accelerated sequencing, reducing the cost to $10,000 and time to less than a week.
This document summarizes an article from The Corporate Governance Advisor on tools for boards to oversee cybersecurity risk. It discusses the business impacts and litigation/regulatory risks of cyber attacks. It outlines how boards have an oversight duty to ensure proper information and reporting systems exist to manage cybersecurity risk. The document provides examples of cybersecurity disclosure from companies like Target and Home Depot. It discusses SEC guidance on cybersecurity disclosure and notes boards must exercise oversight in good faith to avoid liability for failures.
Product Cost Analytics solution overviewSridhar Pai
The document discusses a product cost analytics solution from ConverBiz Technologies. It provides self-service reporting on product cost management data through pre-built dashboards. The solution allows assembly cost analysis, commodity analysis, supplier spend projection, and sourcing performance tracking. Sample dashboards show cost breakdowns, top cost drivers, and supplier spending charts. The solution is built on Oracle Product Lifecycle Analytics and integrates with Oracle Agile Product Cost Management.
East Coast Transport-MACRO POINT CASE STUDYPaul Berman
East Coast Transport, a third-party logistics provider, implemented MacroPoint's freight tracking software to gain real-time visibility of load locations from start to finish. The software allows them to monitor every load with unprecedented precision and measure carrier performance. It has increased the carrier conversion rate to accept tracking from 50% to 80% over two years. MacroPoint saves East Coast Transport time by eliminating the need to continually call carriers and drivers for load updates.
Calendrier des activités de JEADER _ AFRIQUE _ 2016 JEADER
2016 a été une année spéciale grâce à vous ! Merci pour votre support et revisitez les activités phares de 2016.
Ensemble faisons de 2017 une année tout aussi EXTRAORDINAIRE !
Experience the wonders of Sri Lanka from the best individual tour operator with BUDGET PRICES.
TRUST US WITH YOUR HOLIDAYS WE HAVE FOR YOU .WE OFFER A WIDE CHOICE OF TOURS: OUR EXPERTS CAN ALSO “TAILOR “A HOLIDAY TO YOUR PERSONAL WISHES.
Este documento ofrece consejos para periodistas sobre cómo informar de manera objetiva, independiente y ética. En resumen, recomienda: 1) informar sobre los hechos verdaderos y no sobre ficciones o deseos; 2) anteponer la verdad a otras consideraciones y evitar mentiras; 3) ser tan objetivo como un espejo plano al informar.
This document provides an agenda and logistical information for the 2017 Michigan Road Scholars Tour orientation. The tour will take place from May 1-5, 2017 and will involve visits to various locations across Michigan, including Detroit, Flint, Sault Ste. Marie, Traverse City, Grand Rapids, and Lansing. The goals of the tour are to increase understanding of Michigan's economy, culture, and issues through interactions with communities and to encourage collaboration among university faculty. The agenda outlines details of travel arrangements, accommodations, packing suggestions, and activities at each tour location.
A Administração e a Contabilidade : Uma relação que gera vantagem competitiva...Cra-es Conselho
Nesta palestra o Prof. Hercules Vander de Lima Freire fala sobre as diferenças entre as profissões, a Características qualitativas da informação contábil-financeira útil; Características qualitativas fundamentais e Características qualitativas de melhoria.
The document discusses the history and development of DNA sequencing technologies. It describes how Solexa sequencing was invented in 1997 at the University of Cambridge chemistry department and was later sold for £420 million in 2006. Solexa sequencing enabled sequencing a human genome in under a day for around £700, whereas the Human Genome Project cost over £2 billion and took 14 years to complete. The key innovation of Solexa sequencing was its ability to detect single molecules using fluorescence microscopy.
Energy-dispersive x-ray diffraction for on-stream monitoring of mJoel O'Dwyer
This document describes a thesis submitted by Joel N. O'Dwyer to the University of Wollongong in fulfillment of the requirements for a Doctor of Philosophy degree. The thesis develops a new technique called energy-dispersive X-ray diffraction (EDXRD) for monitoring mineralogy in industrial process streams in real-time. Monte Carlo modeling was used to simulate X-ray diffraction and optimize the design of a prototype EDXRD analyzer. Quantitative mineral analysis experiments demonstrated that the technique can determine mineral components in samples with accuracies of better than 1 wt%. The results validate EDXRD as a viable tool for online mineral analysis of process streams.
This document summarizes the history and development of DNA sequencing techniques. It discusses early discoveries about DNA structure in the 1950s-1960s by Watson, Crick, Chargaff, and others. The first genome to be sequenced was phage λ in 1977, which took over 3 years. Sanger developed dideoxy sequencing in 1977 allowing sequencing of ~100 bases per reaction. PCR was developed by Mullis in 1983, enabling amplification of DNA. Automated sequencing machines were developed in the 1980s-1990s allowing sequencing of up to 1000 bases per reaction. The document also discusses DNA polymerase, PCR, and Sanger sequencing techniques in more detail.
The document summarizes key discoveries in establishing DNA as the genetic material. It describes experiments by Griffith, Avery, Hershey and Chase showing that DNA transforms bacteria and is the genetic material in viruses. Watson and Crick developed the double helix model of DNA structure based on Franklin's X-ray images, explaining Chargaff's rules. Their model suggested DNA replication is semiconservative, supported by Meselson-Stahl experiments. DNA polymerase synthesizes new DNA strands while helicase and ligase aid replication.
This document provides an overview of the key discoveries and evidence establishing DNA as the genetic material. It summarizes Griffith's experiments showing bacterial transformation by DNA and Hershey and Chase's experiments using bacteriophages to demonstrate that viral DNA enters host cells to direct new phage production. It also reviews Watson and Crick's proposal of the double helix structure of DNA based on Franklin's X-ray crystallography images, including their explanation of how specific base-pairing of A-T and C-G allows DNA to replicate semi-conservatively as shown by Meselson and Stahl's experiments.
The document provides an overview of the history and scope of bioinformatics. It discusses how bioinformatics emerged from the fields of computer science and biology. The history section outlines major developments from Mendel's work in 1865 to the sequencing of the human genome in 2001. Bioinformatics has various applications in areas like drug development, personalized medicine, and biotechnology. It also has significant scope in India, with growing job opportunities in both the public and private sectors.
DNA was discovered to be the genetic material through a series of experiments in the 1900s. Griffith's experiments with bacteria showed that DNA carries genetic information that can transform non-virulent bacteria strains into virulent ones. Further experiments by Avery, Macleod and McCarty isolated the heat-killed bacteria and found that only the DNA fraction could induce this transformation, not other components like proteins. Hershey and Chase's experiments with bacteriophage also showed that viral DNA, not proteins, entered the bacterial host cell and was responsible for replication. These experiments conclusively demonstrated that DNA is the carrier of genetic information in living organisms.
The document discusses the history and advancements of DNA sequencing. It describes how the discovery of the DNA structure in 1953 enabled sequencing. The first human genome was sequenced from 1977-2003 using Fred Sanger's method and cost $3 billion. New single molecule detection techniques invented in 1997 dramatically accelerated sequencing, reducing the cost to $10,000 and time to less than a week.
This document summarizes an article from The Corporate Governance Advisor on tools for boards to oversee cybersecurity risk. It discusses the business impacts and litigation/regulatory risks of cyber attacks. It outlines how boards have an oversight duty to ensure proper information and reporting systems exist to manage cybersecurity risk. The document provides examples of cybersecurity disclosure from companies like Target and Home Depot. It discusses SEC guidance on cybersecurity disclosure and notes boards must exercise oversight in good faith to avoid liability for failures.
Product Cost Analytics solution overviewSridhar Pai
The document discusses a product cost analytics solution from ConverBiz Technologies. It provides self-service reporting on product cost management data through pre-built dashboards. The solution allows assembly cost analysis, commodity analysis, supplier spend projection, and sourcing performance tracking. Sample dashboards show cost breakdowns, top cost drivers, and supplier spending charts. The solution is built on Oracle Product Lifecycle Analytics and integrates with Oracle Agile Product Cost Management.
East Coast Transport-MACRO POINT CASE STUDYPaul Berman
East Coast Transport, a third-party logistics provider, implemented MacroPoint's freight tracking software to gain real-time visibility of load locations from start to finish. The software allows them to monitor every load with unprecedented precision and measure carrier performance. It has increased the carrier conversion rate to accept tracking from 50% to 80% over two years. MacroPoint saves East Coast Transport time by eliminating the need to continually call carriers and drivers for load updates.
Calendrier des activités de JEADER _ AFRIQUE _ 2016 JEADER
2016 a été une année spéciale grâce à vous ! Merci pour votre support et revisitez les activités phares de 2016.
Ensemble faisons de 2017 une année tout aussi EXTRAORDINAIRE !
Experience the wonders of Sri Lanka from the best individual tour operator with BUDGET PRICES.
TRUST US WITH YOUR HOLIDAYS WE HAVE FOR YOU .WE OFFER A WIDE CHOICE OF TOURS: OUR EXPERTS CAN ALSO “TAILOR “A HOLIDAY TO YOUR PERSONAL WISHES.
Este documento ofrece consejos para periodistas sobre cómo informar de manera objetiva, independiente y ética. En resumen, recomienda: 1) informar sobre los hechos verdaderos y no sobre ficciones o deseos; 2) anteponer la verdad a otras consideraciones y evitar mentiras; 3) ser tan objetivo como un espejo plano al informar.
This document provides an agenda and logistical information for the 2017 Michigan Road Scholars Tour orientation. The tour will take place from May 1-5, 2017 and will involve visits to various locations across Michigan, including Detroit, Flint, Sault Ste. Marie, Traverse City, Grand Rapids, and Lansing. The goals of the tour are to increase understanding of Michigan's economy, culture, and issues through interactions with communities and to encourage collaboration among university faculty. The agenda outlines details of travel arrangements, accommodations, packing suggestions, and activities at each tour location.
A Administração e a Contabilidade : Uma relação que gera vantagem competitiva...Cra-es Conselho
Nesta palestra o Prof. Hercules Vander de Lima Freire fala sobre as diferenças entre as profissões, a Características qualitativas da informação contábil-financeira útil; Características qualitativas fundamentais e Características qualitativas de melhoria.
The document discusses the history and development of DNA sequencing technologies. It describes how Solexa sequencing was invented in 1997 at the University of Cambridge chemistry department and was later sold for £420 million in 2006. Solexa sequencing enabled sequencing a human genome in under a day for around £700, whereas the Human Genome Project cost over £2 billion and took 14 years to complete. The key innovation of Solexa sequencing was its ability to detect single molecules using fluorescence microscopy.
Energy-dispersive x-ray diffraction for on-stream monitoring of mJoel O'Dwyer
This document describes a thesis submitted by Joel N. O'Dwyer to the University of Wollongong in fulfillment of the requirements for a Doctor of Philosophy degree. The thesis develops a new technique called energy-dispersive X-ray diffraction (EDXRD) for monitoring mineralogy in industrial process streams in real-time. Monte Carlo modeling was used to simulate X-ray diffraction and optimize the design of a prototype EDXRD analyzer. Quantitative mineral analysis experiments demonstrated that the technique can determine mineral components in samples with accuracies of better than 1 wt%. The results validate EDXRD as a viable tool for online mineral analysis of process streams.
This document summarizes the history and development of DNA sequencing techniques. It discusses early discoveries about DNA structure in the 1950s-1960s by Watson, Crick, Chargaff, and others. The first genome to be sequenced was phage λ in 1977, which took over 3 years. Sanger developed dideoxy sequencing in 1977 allowing sequencing of ~100 bases per reaction. PCR was developed by Mullis in 1983, enabling amplification of DNA. Automated sequencing machines were developed in the 1980s-1990s allowing sequencing of up to 1000 bases per reaction. The document also discusses DNA polymerase, PCR, and Sanger sequencing techniques in more detail.
The document summarizes key discoveries in establishing DNA as the genetic material. It describes experiments by Griffith, Avery, Hershey and Chase showing that DNA transforms bacteria and is the genetic material in viruses. Watson and Crick developed the double helix model of DNA structure based on Franklin's X-ray images, explaining Chargaff's rules. Their model suggested DNA replication is semiconservative, supported by Meselson-Stahl experiments. DNA polymerase synthesizes new DNA strands while helicase and ligase aid replication.
This document provides an overview of the key discoveries and evidence establishing DNA as the genetic material. It summarizes Griffith's experiments showing bacterial transformation by DNA and Hershey and Chase's experiments using bacteriophages to demonstrate that viral DNA enters host cells to direct new phage production. It also reviews Watson and Crick's proposal of the double helix structure of DNA based on Franklin's X-ray crystallography images, including their explanation of how specific base-pairing of A-T and C-G allows DNA to replicate semi-conservatively as shown by Meselson and Stahl's experiments.
The document provides an overview of the history and scope of bioinformatics. It discusses how bioinformatics emerged from the fields of computer science and biology. The history section outlines major developments from Mendel's work in 1865 to the sequencing of the human genome in 2001. Bioinformatics has various applications in areas like drug development, personalized medicine, and biotechnology. It also has significant scope in India, with growing job opportunities in both the public and private sectors.
DNA was discovered to be the genetic material through a series of experiments in the 1900s. Griffith's experiments with bacteria showed that DNA carries genetic information that can transform non-virulent bacteria strains into virulent ones. Further experiments by Avery, Macleod and McCarty isolated the heat-killed bacteria and found that only the DNA fraction could induce this transformation, not other components like proteins. Hershey and Chase's experiments with bacteriophage also showed that viral DNA, not proteins, entered the bacterial host cell and was responsible for replication. These experiments conclusively demonstrated that DNA is the carrier of genetic information in living organisms.
This document provides an overview of DNA microarray technology. It discusses the historical background beginning in the 1970s with Southern blotting and the development of microarrays in the 1980s. The key principles are that DNA microarrays allow analysis of thousands of genes simultaneously and efficiently through orderly arrangement of DNA sequences on a solid surface like glass. The main steps involve preparing the microarray slide through various methods, performing experiments with sample mRNA, fluorescence scanning, and data analysis to understand gene expression patterns. DNA microarray technology has wide applications in studying diseases, toxicology, and stem cell research.
The document describes DNA structure and replication. It begins by discussing early evidence that DNA is the genetic material, including Griffith's experiments showing bacterial transformation and Hershey and Chase's experiments tracing phage DNA and proteins during infection. Watson and Crick then developed the first accurate double-helix model of DNA structure in 1953 based on Franklin's X-ray crystallography images, with base-pairing of A-T and C-G. The document concludes by explaining that DNA replication involves unwinding the double helix and using each strand as a template to build new complementary strands based on base-pairing rules, with many proteins involved.
Validating and improving the D. melanogaster reference genome sequence using ...Casey Bergman
1) The document discusses using PacBio long read sequencing to improve the Drosophila melanogaster reference genome assembly.
2) PacBio sequencing generated over 15 billion bases of long read data, which was used to assemble the genome into contigs over 15 Mb in length with fewest errors compared to the reference.
3) The PacBio assemblies helped close gaps in the reference genome and generated large contigs from heterochromatic regions that were previously unassembled.
1) James Watson and Francis Crick discovered the double helix structure of DNA in 1953, which showed that DNA is the genetic material that directs the inheritance of traits.
2) Experiments in the 1940s-1950s provided evidence that DNA, not protein, was the genetic material: Avery, McCarty, and MacLeod showed DNA was the transforming principle in bacteria; Hershey and Chase showed that DNA, not protein, enters the host cell during bacterial virus infection.
3) Watson and Crick developed the double helix model of DNA structure in 1953 based on evidence such as Chargaff's rules of base pairing and X-ray crystallography images from Franklin - their model explained
1) DNA was identified as the genetic material through experiments in the 1940s-1950s studying bacteria, viruses, and their ability to transform cells.
2) Watson and Crick developed the double helix model of DNA structure in 1953 based on evidence including X-ray crystallography images that showed DNA had a regular helical structure.
3) DNA replication is semi-conservative and involves unwinding the DNA double helix, synthesizing new strands based on base-pairing rules, and producing two identical copies of the original DNA molecule before cell division.
DNA replication is a highly regulated process that occurs semiconservatively to produce two identical copies of DNA during cell division. It begins at origins of replication where the double helix unwinds into single strands. One strand serves as the template for continuous synthesis of the leading strand while the lagging strand is synthesized discontinuously in short segments called Okazaki fragments that are later joined. DNA polymerases add nucleotides to growing strands based on base pairing rules, while other proteins such as helicases and ligases facilitate the process to maintain fidelity of the genetic code.
This document provides information about EMBL, CLUSTALW, and how multiple sequence alignment can be performed using CLUSTALW. EMBL is a leading life sciences research institution located in the UK. CLUSTALW is a multiple sequence alignment software produced in 1994 that uses seeded guide trees and HMM profile-profile alignment. The document then describes how to use NCBI and BLAST to retrieve gene sequences and perform multiple sequence alignment using CLUSTALW online to analyze gene sequences and their phylogenetic relationships.
DNA sequencing: rapid improvements and their implicationsJeffrey Funk
these slides analyze the rapid improvements in DNA sequencers and the implications for these rapid improvements for drug discovery, new crops, materials creation, and new bio-fuels. Many of the rapid improvements are from "reductions in scale." As with integrated circuits, reducing the size of features on DNA sequencers has enabled many orders of magnitude improvements in them. Unlike integrated circuits, the improvements are also due to changes in technology. For example, changes from pyrosequencing to semiconductor and nanopore sequencing have also been needed to achieve the reductions in scale. Second, pyrosequencing also benefited from improvements in lasers and camera chips.
First, SANS can provide structural information on a wide range of length scales making it useful for studying complex biological systems. However, while time-of-flight SANS has advantages for biology, few examples currently exist where it has been used for model building. This is due to issues with the source strength, background subtraction, and data reduction for TOF-SANS instruments. The new SANS2D diffractometer shows promise as a versatile TOF-SANS instrument for structural biology once these technical challenges are addressed.
We've long wondered if we're along in the universe. There's more and more tantalizing hints that we might not be alone in our own solar system. The default assumption is that those "hints" are weird chemistry or bad measurements or modeling, but if that's not the case, what then?
- The document discusses DNA structure and replication. It describes key experiments that helped establish DNA as the genetic material, including Griffith's transformation experiments with bacteria and Hershey and Chase's experiments with bacteriophage. It then explains Watson and Crick's discovery of the double helix structure of DNA and how this explained the rules of base pairing. Finally, it provides details on the semi-conservative mechanism of DNA replication and how leading and lagging strands are synthesized.
Immersive Learning That Works: Research Grounding and Paths ForwardLeonel Morgado
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
Mechanisms and Applications of Antiviral Neutralizing Antibodies - Creative B...Creative-Biolabs
Neutralizing antibodies, pivotal in immune defense, specifically bind and inhibit viral pathogens, thereby playing a crucial role in protecting against and mitigating infectious diseases. In this slide, we will introduce what antibodies and neutralizing antibodies are, the production and regulation of neutralizing antibodies, their mechanisms of action, classification and applications, as well as the challenges they face.
PPT on Sustainable Land Management presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
TOPIC OF DISCUSSION: CENTRIFUGATION SLIDESHARE.pptxshubhijain836
Centrifugation is a powerful technique used in laboratories to separate components of a heterogeneous mixture based on their density. This process utilizes centrifugal force to rapidly spin samples, causing denser particles to migrate outward more quickly than lighter ones. As a result, distinct layers form within the sample tube, allowing for easy isolation and purification of target substances.
CLASS 12th CHEMISTRY SOLID STATE ppt (Animated)eitps1506
Description:
Dive into the fascinating realm of solid-state physics with our meticulously crafted online PowerPoint presentation. This immersive educational resource offers a comprehensive exploration of the fundamental concepts, theories, and applications within the realm of solid-state physics.
From crystalline structures to semiconductor devices, this presentation delves into the intricate principles governing the behavior of solids, providing clear explanations and illustrative examples to enhance understanding. Whether you're a student delving into the subject for the first time or a seasoned researcher seeking to deepen your knowledge, our presentation offers valuable insights and in-depth analyses to cater to various levels of expertise.
Key topics covered include:
Crystal Structures: Unravel the mysteries of crystalline arrangements and their significance in determining material properties.
Band Theory: Explore the electronic band structure of solids and understand how it influences their conductive properties.
Semiconductor Physics: Delve into the behavior of semiconductors, including doping, carrier transport, and device applications.
Magnetic Properties: Investigate the magnetic behavior of solids, including ferromagnetism, antiferromagnetism, and ferrimagnetism.
Optical Properties: Examine the interaction of light with solids, including absorption, reflection, and transmission phenomena.
With visually engaging slides, informative content, and interactive elements, our online PowerPoint presentation serves as a valuable resource for students, educators, and enthusiasts alike, facilitating a deeper understanding of the captivating world of solid-state physics. Explore the intricacies of solid-state materials and unlock the secrets behind their remarkable properties with our comprehensive presentation.
Discovery of An Apparent Red, High-Velocity Type Ia Supernova at 𝐳 = 2.9 wi...Sérgio Sacani
We present the JWST discovery of SN 2023adsy, a transient object located in a host galaxy JADES-GS
+
53.13485
−
27.82088
with a host spectroscopic redshift of
2.903
±
0.007
. The transient was identified in deep James Webb Space Telescope (JWST)/NIRCam imaging from the JWST Advanced Deep Extragalactic Survey (JADES) program. Photometric and spectroscopic followup with NIRCam and NIRSpec, respectively, confirm the redshift and yield UV-NIR light-curve, NIR color, and spectroscopic information all consistent with a Type Ia classification. Despite its classification as a likely SN Ia, SN 2023adsy is both fairly red (
�
(
�
−
�
)
∼
0.9
) despite a host galaxy with low-extinction and has a high Ca II velocity (
19
,
000
±
2
,
000
km/s) compared to the general population of SNe Ia. While these characteristics are consistent with some Ca-rich SNe Ia, particularly SN 2016hnk, SN 2023adsy is intrinsically brighter than the low-
�
Ca-rich population. Although such an object is too red for any low-
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cosmological sample, we apply a fiducial standardization approach to SN 2023adsy and find that the SN 2023adsy luminosity distance measurement is in excellent agreement (
≲
1
�
) with
Λ
CDM. Therefore unlike low-
�
Ca-rich SNe Ia, SN 2023adsy is standardizable and gives no indication that SN Ia standardized luminosities change significantly with redshift. A larger sample of distant SNe Ia is required to determine if SN Ia population characteristics at high-
�
truly diverge from their low-
�
counterparts, and to confirm that standardized luminosities nevertheless remain constant with redshift.
Anti-Universe And Emergent Gravity and the Dark UniverseSérgio Sacani
Recent theoretical progress indicates that spacetime and gravity emerge together from the entanglement structure of an underlying microscopic theory. These ideas are best understood in Anti-de Sitter space, where they rely on the area law for entanglement entropy. The extension to de Sitter space requires taking into account the entropy and temperature associated with the cosmological horizon. Using insights from string theory, black hole physics and quantum information theory we argue that the positive dark energy leads to a thermal volume law contribution to the entropy that overtakes the area law precisely at the cosmological horizon. Due to the competition between area and volume law entanglement the microscopic de Sitter states do not thermalise at sub-Hubble scales: they exhibit memory effects in the form of an entropy displacement caused by matter. The emergent laws of gravity contain an additional ‘dark’ gravitational force describing the ‘elastic’ response due to the entropy displacement. We derive an estimate of the strength of this extra force in terms of the baryonic mass, Newton’s constant and the Hubble acceleration scale a0 = cH0, and provide evidence for the fact that this additional ‘dark gravity force’ explains the observed phenomena in galaxies and clusters currently attributed to dark matter.
(June 12, 2024) Webinar: Development of PET theranostics targeting the molecu...Scintica Instrumentation
Targeting Hsp90 and its pathogen Orthologs with Tethered Inhibitors as a Diagnostic and Therapeutic Strategy for cancer and infectious diseases with Dr. Timothy Haystead.
2. The other Director of Studies in Chemistry
Available on:
iTunes (https://goo.gl/hwMEFG)
SoundCloud (https://goo.gl/s8p8Vd)
YouTube
(www.youtube.com/channel/UCWv6XjH8pzm
WMVuE_pwz1mg)
Twitter: @SteveTheChemist and
@theevanslab.
3. Solexa sequencing
• Invented in 1997 in the
chemistry department by David
Klenerman and Shankar
Balasubramanian
• Developed by a spin-out
company in Saffron Walden
4. Solexa sequencing
• Invented in 1997 in the
chemistry department by David
Klenerman and Shankar
Balasubramanian
• Developed by a spin-out
company in Saffron Walden
• Sold for £420,000,000 in 2006
5. Solexa sequencing
• Invented in 1997 in the
chemistry department by David
Klenerman and Shankar
Balasubramanian
• Developed by a spin-out
company in Saffron Walden
The latest version of the DNA sequencer they
invented
(costs about £6,000,000)
• Sold for £420,000,000 in 2006
7. Locations and timeline
1 mile
http://www.cambridge
2000.com
Old Cavendish
Laboratory
1953: Discovery of the
structure of DNA
8. Locations and timeline
1 mile
http://www.cambridge
2000.com
Old Cavendish
Laboratory
1953: Discovery of the
structure of DNA
LMB
1977: Sanger method for
sequencing invented
http://www2.mrc-lmb.cam.ac.uk
9. Locations and timeline
1 mile
http://www.cambridge
2000.com
Old Cavendish
Laboratory
1953: Discovery of the
structure of DNA
Sanger
Institute
1993: Work on Human
genome project at the
Sanger starts
Genome Research Ltd.
LMB
1977: Sanger method for
sequencing invented
http://www2.mrc-lmb.cam.ac.uk
10. Locations and timeline
1 mile
http://www.cambridge
2000.com
Old Cavendish
Laboratory
1953: Discovery of the
structure of DNA
Chemistry
department
http://www.flickr.com/
photos/shai-
bl/5584629687/sizes/
m/in/photostream/
1997: Work on Solexa method
for sequencing started
Sanger
Institute
1993: Work on Human
genome project at the
Sanger starts
Genome Research Ltd.
LMB
1977: Sanger method for
sequencing invented
http://www2.mrc-lmb.cam.ac.uk
11. Structure of DNA
http://www.themicrobiologist.com
Solved in Cambridge in 1953 by James Watson
and Francis Crick using data collected by Rosalind
Franklin and Maurice Wilkins at King’s College
London
The key to the structure was base pairing
12. Structure of DNA
http://www.themicrobiologist.com
Solved in Cambridge in 1953 by James Watson
and Francis Crick using data collected by Rosalind
Franklin and Maurice Wilkins at King’s College
London
The key to the structure was base pairing
17. DNA Sequencing
Why would you want to sequence DNA?
A genome contains the information
required to build an organism
18. DNA Sequencing
Why would you want to sequence DNA?
A genome contains the information
required to build an organism
It’s a long book...
Wikipedia
19. DNA Sequencing
Why would you want to sequence DNA?
A genome contains the information
required to build an organism
It’s a long book...
~6,000,000,000 (6 ×109)
letters in most of the
~4×1013 cells in a human
Wikipedia
20. DNA Sequencing
Why would you want to sequence DNA?
A genome contains the information
required to build an organism
It’s a long book...
~6,000,000,000 (6 ×109)
letters in most of the
~4×1013 cells in a human
Distance between base pairs
= 0.34 nm (0.34 ×10-9 m)
Wikipedia
21. DNA Sequencing
Why would you want to sequence DNA?
A genome contains the information
required to build an organism
It’s a long book...
~6,000,000,000 (6 ×109)
letters in most of the
~4×1013 cells in a human
The DNA in one of your cells would be 2 m long in the B-form structure
Distance between base pairs
= 0.34 nm (0.34 ×10-9 m)
Wikipedia
29. T
Sanger sequencing
CAGTCAGTCA
T GCG A
Based on copying of DNA:
Genome Research Ltd.
GA
C
G
A
C
TA
T
C
Incorporating a fluorescent
nucleotide stops the copying
59. The human genome project
Cost: £2,000,000,000
First draft completed: 2000
‘Finished’: 2003
http://www.c-spanvideo.org/program/157909-1
Started: 1989 (in the USA)
60. The human genome project
Cost: £2,000,000,000
First draft completed: 2000
‘Finished’: 2003
http://www.c-spanvideo.org/program/157909-1
Started: 1989 (in the USA)
“Without a doubt, this is the most important,
most wondrous map ever produced by
humankind” W.J. Clinton, 26/06/2000
61. The human genome project
Cost: £2,000,000,000
First draft completed: 2000
‘Finished’: 2003
http://www.flickr.com/photos/93425126@N00/43948
34217/in/set-72157623515077498/
http://www.c-spanvideo.org/program/157909-1
Started: 1989 (in the USA)
UK effort on the Human Genome Project largely carried
out in this building in the Sanger Centre
9 Chromosomes were sequenced here (about
a third of the genome)
“Without a doubt, this is the most important,
most wondrous map ever produced by
humankind” W.J. Clinton, 26/06/2000
62. Solexa sequencing
• Cost to sequence a human
genome: around £700 (compared
to £2,000,000,000)
• Time to sequence a human
genome: less than a day
(compared to 14 years)
• Based on a microscope that can
detect single molecules
https://www.genome.gov/sequencingcosts/
Solexa’s first
sequencer
launched
Illumina
buys
Solexa
70. Looking for a needle in a haystack?
What’s so impressive about detecting a single
molecule?
71. Looking for a needle in a haystack?
How many blades of grass on a football pitch?
What’s so impressive about detecting a single
molecule?
72. Looking for a needle in a haystack?
About 200,000,000 or 2×108
How many blades of grass on a football pitch?
What’s so impressive about detecting a single
molecule?
73. How many molecules in a vial of water?
Looking for a needle in a haystack?
About 200,000,000 or 2×108
How many blades of grass on a football pitch?
What’s so impressive about detecting a single
molecule?
74. 18 mL (1 mole) of water contains Avogadro’s
number of molecules: 6.02 ×1023
How many molecules in a vial of water?
Looking for a needle in a haystack?
About 200,000,000 or 2×108
How many blades of grass on a football pitch?
What’s so impressive about detecting a single
molecule?
75. 18 mL (1 mole) of water contains Avogadro’s
number of molecules: 6.02 ×1023
How many molecules in a vial of water?
Looking for a needle in a haystack?
About 200,000,000 or 2×108
How many blades of grass on a football pitch?
So 1 mole of grass blades would cover
6.02 ×1023 ÷ 2×108 = 3 ×1015 football pitches
What’s so impressive about detecting a single
molecule?
76. 18 mL (1 mole) of water contains Avogadro’s
number of molecules: 6.02 ×1023
How many molecules in a vial of water?
Looking for a needle in a haystack?
About 200,000,000 or 2×108
How many blades of grass on a football pitch?
So 1 mole of grass blades would cover
6.02 ×1023 ÷ 2×108 = 3 ×1015 football pitches
That’s a lot of haystacks...
What’s so impressive about detecting a single
molecule?
77. 1 mole of grass blades = 3×1015 football pitches = 15×1012 km2
What’s so impressive about detecting a single
molecule?
78. 1 mole of grass blades = 3×1015 football pitches = 15×1012 km2
Surface area of Earth = 5×108 km2
(1011 football pitches!)
What’s so impressive about detecting a single
molecule?
79. 1 mole of grass blades = 3×1015 football pitches = 15×1012 km2
Surface area of Jupiter = 6×1010 km2
What’s so impressive about detecting a single
molecule?
80. 1 mole of grass blades = 3×1015 football pitches = 15×1012 km2
Surface area of the Sun
= 6×1012 km2
What’s so impressive about detecting a single
molecule?
81. 1 mole of grass blades = 3×1015 football pitches = 15×1012 km2
Surface area of the Sun
= 6×1012 km2
1 mole of grass
blades would
cover the
surface area of
about 2.5 Suns!
What’s so impressive about detecting a single
molecule?
82. 1 mole of grass blades = 3×1015 football pitches = 15×1012 km2
Surface area of the Sun
= 6×1012 km2
1 mole of grass
blades would
cover the
surface area of
about 2.5 Suns!
All images: nasa.gov
What’s so impressive about detecting a single
molecule?
85. Solexa sequencing
Solexa sequencing uses about 103 molecules to read
100 letters
About as many blades of grass as on the penalty spot
Imaging technology: Total Internal Reflection
Fluorescence (TIRF) microscopy
http://thesportboys.wordpress.com/category/international/page/2/
100. Summary
The structure of DNA, discovered in 1953 has been crucial to sequencing the human genome
The first human genome was sequenced using Fred Sanger’s method, invented in 1977. The
project ran for 14 years, costing £2 billion
New methods for sequencing use single-molecule detection to dramatically accelerate the
decoding process
One approach using single molecule techniques, invented by Shankar Balasubramanian and
David Klenerman in the chemistry department in 1997 is now widely used for sequencing
worldwide
The cost of sequencing has fallen to £700 and takes less than a day