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Which is NOT true for solute retention?
A) Retention time, t r, is the elapsed time between injecting the sample to the arrival of a sample
component at the detector minus the dwell time between the end of the column and the detector.
B) Adjusted retention time is t r’ = t r – t m, where t r is the retention time and t m is the
time for mobile phase or unretained solutes to pass through the column.
C) The relative retention, ? = r 2 , is the ratio for the adjusted retention time for two t ' r1
components of the sample, where t r2’ > t r1’ so that ? > 1.
D) The retention factor, k = t r ? t m , is the time, tr, required to elute a component
t m
K 1coefficients for components 2 and 1.minus the time, tm, for mobile phase to pass through the
column divided by t m .
E) The relative retention, ?, also equals K 2 , where K 2 and K 1 are the partition coefficients for
components 2 and 1.
Solution
(A) is the answer.
Reason: The absolute time taken by a compound to travel from the injector through the column
to the detector is the retention time.
Dwell time will not come into the definition.
Rest all options are correct
Which is NOT true for solute retention- A) Retention time- tr- is the.docx

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Which is NOT true for solute retention- A) Retention time- tr- is the.docx

  • 1. Which is NOT true for solute retention? A) Retention time, t r, is the elapsed time between injecting the sample to the arrival of a sample component at the detector minus the dwell time between the end of the column and the detector. B) Adjusted retention time is t r’ = t r – t m, where t r is the retention time and t m is the time for mobile phase or unretained solutes to pass through the column. C) The relative retention, ? = r 2 , is the ratio for the adjusted retention time for two t ' r1 components of the sample, where t r2’ > t r1’ so that ? > 1. D) The retention factor, k = t r ? t m , is the time, tr, required to elute a component t m K 1coefficients for components 2 and 1.minus the time, tm, for mobile phase to pass through the column divided by t m . E) The relative retention, ?, also equals K 2 , where K 2 and K 1 are the partition coefficients for components 2 and 1. Solution (A) is the answer. Reason: The absolute time taken by a compound to travel from the injector through the column to the detector is the retention time. Dwell time will not come into the definition. Rest all options are correct