Cell lines are established cell cultures that can proliferate indefinitely in vitro. They are used in basic research and early drug development studies. Cell lines are categorized as finite or continuous based on their lifespan. Finite cell lines have limited proliferation while continuous cell lines can proliferate indefinitely. Characterization of cell lines is important to determine their species of origin, tissue of origin, and genetic stability. Common cell lines used in research include human cell lines like HeLa, mouse cell lines like 3T3 fibroblasts, and rat cell lines. Cell lines are useful for investigating disease pathways and developing therapies.