This document presents a validated RP-HPLC method for the estimation of Liraglutide in tablet dosage forms. The method utilizes a C18 column with a mobile phase of 0.1% ortho phosphoric acid, acetonitrile and methanol. Liraglutide was detected at 245nm with a retention time of 4.447 minutes. The method was validated in terms of linearity, precision, accuracy and specificity according to ICH guidelines. The method was successfully applied to analyze Liraglutide levels in commercial tablets.
Hplc method development for proteins and polypeptides ijsit 2.4.2IJSIT Editor
In the pharmaceutical field, there is considerable interest in the use of peptides and proteins for therapeutic
purposes. High performance liquid chromatography (HPLC) and its methods of complex peptide or protein
mixtures remains a general method of choice because of the resolution it provides. Unlike small organic
molecules whose chromatographic behavior is described by a finite partitioning equilibrium between the
stationary phase and the mobile phase, proteins and peptides typically do not exhibit such an effect. Instead,
they exhibit an adsorption phenomenon in which the polypeptide is adsorbed onto the stationary phase and
elutes only when the solvent strength of the mobile phase is sufficient to compete with the hydrophobic
forces keeping it there. For this reason, elution of peptides or proteins from reversed-phase supports is by
gradients of increasing solvent strength. There are other differences that one needs to be aware of in order to
develop HPLC methods for separations of proteins and peptides as efficiently as possible.
Electrophoresis is a technique used to separate macromolecules like proteins, DNA, and RNA based on their size and charge. There are two main types: free electrophoresis and zone electrophoresis. Zone electrophoresis is more commonly used and separates particles into zones or bands within a stabilizing medium like a gel or paper. Different electrophoretic techniques exist like polyacrylamide gel electrophoresis and agarose gel electrophoresis which are used to separate biomolecules and help study properties like protein structure and bacterial antibiotic resistance. Electrophoresis has applications in separating complex molecule mixtures and analyzing nucleic acids.
The document discusses capillary electrophoresis (CE), including its key terminology, instrumentation, flow dynamics, and factors that affect separation efficiency such as capillary diameter, voltage, and temperature. CE uses narrow capillaries to perform high-efficiency separations of charged molecules. When an electric field is applied, electroosmotic flow and electrophoretic migration move solutes through the capillary at different rates depending on their size and charge. Precise temperature control and optimization of factors like voltage and capillary diameter are important for achieving high resolution separations.
The document discusses drug-excipient compatibility studies, which are important to understand interactions between active pharmaceutical ingredients and excipients. There are three main types of incompatibility - physical, chemical, and therapeutic. Compatibility studies help identify incompatible excipients, ensure excipients do not impact drug stability, and can help stabilize unstable drugs. Methods to study compatibility include thermal techniques like DSC, spectroscopic techniques, microscopy, and chromatography. The goal is to avoid issues with drug stability and efficacy during storage and use.
In this slide contains types of HPLC Columns, Plate theory and Van Deemter Equation.
Presented by : Malarvannan.M (Department of pharmaceutical analysis).
RIPER,anantpur.
Introduction to Concepts of Similarity and Difference factors,
Importance of dissolution profile comparison,
Objective of dissolution profile comparison,
Method to compare dissolution profile , f1 & f2 Comparison
Presented By
N. Poojitha
Department of Pharmaceutics
Protein and-peptide-drug-delivery-systemsGaurav Kr
The document discusses protein and peptide drug delivery systems. It begins by defining proteins and peptides, noting that proteins are molecules composed of over 50 amino acids, while peptides are molecules composed of less than 50 amino acids. It then discusses how scientific advances in molecular and cell biology have led to the development of recombinant DNA and hybridoma technology to produce protein products. The document provides examples of marketed protein and peptide drugs and discusses challenges with delivering these drugs orally due to their large molecular size and susceptibility to enzymatic degradation. It explores approaches to protein and peptide delivery including non-parenteral systemic delivery methods and various considerations for developing delivery systems for these pharmaceuticals.
EXPERIMENT PARAMETERS OF DIFFERENTIAL SCANNING CALORIMETRY (DSC)Shikha Popali
THE EXPERIMENTAL PARAMETERS USED IN DSC INCLUDING SAMPLE PREPARATION , EXPERIMENTAL CONDITIONS, CALIBRATION OF APPARATUS, INSTRUMENTS, HEATING RATES AND TEMPERATURES, COOLING RATES,RESOLUTION, ALSO SOURCE OF ERRORS.
Hplc method development for proteins and polypeptides ijsit 2.4.2IJSIT Editor
In the pharmaceutical field, there is considerable interest in the use of peptides and proteins for therapeutic
purposes. High performance liquid chromatography (HPLC) and its methods of complex peptide or protein
mixtures remains a general method of choice because of the resolution it provides. Unlike small organic
molecules whose chromatographic behavior is described by a finite partitioning equilibrium between the
stationary phase and the mobile phase, proteins and peptides typically do not exhibit such an effect. Instead,
they exhibit an adsorption phenomenon in which the polypeptide is adsorbed onto the stationary phase and
elutes only when the solvent strength of the mobile phase is sufficient to compete with the hydrophobic
forces keeping it there. For this reason, elution of peptides or proteins from reversed-phase supports is by
gradients of increasing solvent strength. There are other differences that one needs to be aware of in order to
develop HPLC methods for separations of proteins and peptides as efficiently as possible.
Electrophoresis is a technique used to separate macromolecules like proteins, DNA, and RNA based on their size and charge. There are two main types: free electrophoresis and zone electrophoresis. Zone electrophoresis is more commonly used and separates particles into zones or bands within a stabilizing medium like a gel or paper. Different electrophoretic techniques exist like polyacrylamide gel electrophoresis and agarose gel electrophoresis which are used to separate biomolecules and help study properties like protein structure and bacterial antibiotic resistance. Electrophoresis has applications in separating complex molecule mixtures and analyzing nucleic acids.
The document discusses capillary electrophoresis (CE), including its key terminology, instrumentation, flow dynamics, and factors that affect separation efficiency such as capillary diameter, voltage, and temperature. CE uses narrow capillaries to perform high-efficiency separations of charged molecules. When an electric field is applied, electroosmotic flow and electrophoretic migration move solutes through the capillary at different rates depending on their size and charge. Precise temperature control and optimization of factors like voltage and capillary diameter are important for achieving high resolution separations.
The document discusses drug-excipient compatibility studies, which are important to understand interactions between active pharmaceutical ingredients and excipients. There are three main types of incompatibility - physical, chemical, and therapeutic. Compatibility studies help identify incompatible excipients, ensure excipients do not impact drug stability, and can help stabilize unstable drugs. Methods to study compatibility include thermal techniques like DSC, spectroscopic techniques, microscopy, and chromatography. The goal is to avoid issues with drug stability and efficacy during storage and use.
In this slide contains types of HPLC Columns, Plate theory and Van Deemter Equation.
Presented by : Malarvannan.M (Department of pharmaceutical analysis).
RIPER,anantpur.
Introduction to Concepts of Similarity and Difference factors,
Importance of dissolution profile comparison,
Objective of dissolution profile comparison,
Method to compare dissolution profile , f1 & f2 Comparison
Presented By
N. Poojitha
Department of Pharmaceutics
Protein and-peptide-drug-delivery-systemsGaurav Kr
The document discusses protein and peptide drug delivery systems. It begins by defining proteins and peptides, noting that proteins are molecules composed of over 50 amino acids, while peptides are molecules composed of less than 50 amino acids. It then discusses how scientific advances in molecular and cell biology have led to the development of recombinant DNA and hybridoma technology to produce protein products. The document provides examples of marketed protein and peptide drugs and discusses challenges with delivering these drugs orally due to their large molecular size and susceptibility to enzymatic degradation. It explores approaches to protein and peptide delivery including non-parenteral systemic delivery methods and various considerations for developing delivery systems for these pharmaceuticals.
EXPERIMENT PARAMETERS OF DIFFERENTIAL SCANNING CALORIMETRY (DSC)Shikha Popali
THE EXPERIMENTAL PARAMETERS USED IN DSC INCLUDING SAMPLE PREPARATION , EXPERIMENTAL CONDITIONS, CALIBRATION OF APPARATUS, INSTRUMENTS, HEATING RATES AND TEMPERATURES, COOLING RATES,RESOLUTION, ALSO SOURCE OF ERRORS.
Paper electrophoresis is a technique used to separate charged molecules like amino acids, peptides, and proteins. It works by applying an electric current across a paper strip soaked in buffer solution, which causes the charged molecules in a sample to migrate across the paper at different rates based on their charge and size. The document discusses the principle, requirements, procedure, advantages, types (based on voltage and design), and factors affecting migration of molecules in paper electrophoresis. It is a simple, inexpensive, and widely-used method to qualitatively analyze biological samples for clinical diagnosis and research purposes.
Here are the key IR frequencies identified in the sample that match the reference standard of propafenone:
- C-C stretch at 1186 cm-1
- C=C stretch at 1651 cm-1
- C-H stretch (symmetric) at 2939 cm-1
- C-H bend at 1328 cm-1
- CH2 stretch (symmetric) at 1369 cm-1
- CH2 bend at 1485 cm-1
- CH3 bend at 1398 cm-1
- C-O stretch at 1100 cm-1
- C=O stretch at 1695 cm-1
- N-H stretch at 3417 cm-1
The IR spectrum
Introduction to Higuchi plots for tablet dissolution
Dissolution, Dissolution Models, Higuchi Plot
Presented by
Mohamed Omar Mahmoud
Department of Pharmaceutics
This document discusses modern pharmaceutical analytical techniques, specifically X-ray diffraction and crystallography. It defines X-ray crystallography and X-ray diffraction, explaining how crystal structures cause diffraction patterns. It describes the seven crystal systems and 14 Bravais lattices that define crystal structures. It outlines several applications of X-ray diffraction, including determining unknown crystal structures, analyzing polymers and metals, measuring particle size, and studying complexes.
This document discusses drug-excipient compatibility studies. It begins by explaining the importance of these studies in maximizing dosage form stability and avoiding formulation problems. It then describes the goals of compatibility studies and various mechanisms of drug-excipient interactions including physical, chemical, and physiological interactions. Finally, it outlines several analytical methods used in compatibility studies, including thermal techniques like DSC, spectroscopic techniques like vibrational spectroscopy, and chromatographic techniques like HPLC.
FORMULATION AND EVALUATION OF GELATIN MICROSPHERES LOADED WITH FENOFIBRATEReshma Fathima .K
The document summarizes the formulation and evaluation of gelatin microspheres loaded with the drug Fenofibrate. Two microsphere formulations were developed using a coacervation and phase separation method. Formulation F2 showed 97% drug encapsulation efficiency and released the drug over 12 hours, indicating it was suitable for oral sustained release. Evaluation tests on the microspheres showed they were spherical in shape, had good flow properties, and released the drug in a controlled manner without any burst release. The microspheres could facilitate the design of hard gelatin capsules for improved patient compliance.
Brain targeted drug delivery system seminar.pptxShamsElfalah
This document discusses brain targeted drug delivery systems and strategies to overcome the blood brain barrier (BBB). It begins with an introduction to the challenges of delivering drugs to the central nervous system (CNS) due to the BBB. It then describes the structure and function of the BBB. The rest of the document outlines various approaches to enhance drug delivery to the brain, including invasive techniques like implants and infusion, non-invasive methods like prodrugs and nanoparticles, and miscellaneous techniques. The goal is to review strategies that have been developed to help drugs cross the BBB and treat CNS disorders.
Gel chromatography, also known as gel permeation chromatography or size exclusion chromatography, separates molecules based on their size. It uses columns packed with porous beads that allow small molecules to enter the pores while excluding larger ones, resulting in smaller molecules traveling slower through the column. It is commonly used to determine the molecular weight distributions of polymers.
Dissolution is a test used to evaluate the rate of release of a drug substance from a dosage form. It assesses the performance of drug products and aims to be predictive of bioavailability. The first dissolution studies were reported in 1897 and focused on relating dissolution to drug absorption. Key factors that can affect dissolution include the apparatus used, temperature, media composition and pH, as well as formulation aspects like excipients. Proper procedure involves setting standardized conditions for the apparatus, media preparation, running the test, and calculating the percentage of drug dissolved within a specified time period.
This document discusses in-vivo methods for determining drug permeability and absorption. It describes two main types of in-vivo methods: direct and indirect. The direct method involves measuring drug levels in blood or urine over time after administration to determine the rate and extent of absorption. The indirect method measures pharmacological response instead of drug concentration when direct measurement is difficult. In-vivo methods provide a more precise understanding of factors like gastric emptying and drug effects on the gastrointestinal tract compared to in-vitro or in situ methods.
In vitro dissolution, Alternative Methods.pptxPrachi Pandey
In vitro dissolution and drug release testing provides important information about drug product performance. There are several methods for testing, including sink and non-sink methods as well as natural convection, forced convection, and continuous flow/through methods. Key factors in testing include the dissolution medium, apparatus used, sampling timepoints, and analytical methods for quantifying the amount of drug released over time. Dissolution and drug release profiles are important for understanding and comparing the in vivo behavior and therapeutic effectiveness of different drug product formulations.
ultra high performance liquid chromatographyacademic
This document provides an overview of ultra high performance liquid chromatography (UHPLC). It begins with an introduction defining UHPLC and explaining that it improves resolution, speed and sensitivity compared to traditional HPLC. The document then covers the principles of UHPLC, provides a comparison of UHPLC and HPLC parameters, describes UHPLC instrumentation including pumps, columns and detectors, and discusses applications and advantages/disadvantages of UHPLC.
Barriers to Protein and peptide drug delivery system JaskiranKaur72
Protein and peptide DDS are novel systems of drug delivery.
The successful delivery of peptide and protein-based pharmaceuticals is primarily determined by its ability to cross the various barriers presented to it in the biological milieu. Various barriers encountered are-
1 Physiological Barrier
2 Intestinal Epithelial barriers
3 Capillary Endothelial Barrier
4 Blood-Brain barrier (BBB)
The document discusses the effect of various parameters on drug dissolution. It describes how agitation, pH, viscosity, temperature and other properties of the dissolution medium influence the dissolution rate. The amount of dissolution medium and maintenance of sink conditions are also important. Various dissolution models like Hixson-Crowell are presented.
The document provides guidelines on validation of analytical procedures from the International Conference on Harmonisation (ICH) and the World Health Organization (WHO). It discusses validation characteristics like accuracy, precision, specificity, linearity, range, detection limit and quantitation limit that should be considered when validating identification tests, assays, and tests for impurities. It provides definitions for key terms and recommendations on how validation of these characteristics should be performed.
The document discusses atomic absorption spectroscopy. It begins with an introduction describing how atomic absorption spectroscopy measures the concentration of an element by measuring the amount of light absorbed at a characteristic wavelength when it passes through atoms of that element. It then describes the principle, instrumentation, applications, and sources of interference in atomic absorption spectroscopy. The key sources of interference discussed are non-spectral interferences such as matrix, chemical, and ionization interferences and spectral interferences such as background absorption.
This document discusses methods of formulating and evaluating buccal drug delivery systems. It describes the basic structure and designs of buccal dosage forms as being matrix or reservoir types. The key components are outlined as the drug substance, bioadhesive polymers, backing membrane, and permeation enhancers. Various formulation methods are provided for solid, semi-solid and liquid buccal dosage forms including tablets, patches, films, gels and sprays. Evaluation methods are also summarized such as weight variation, thickness, friability, hardness, and in-vitro swelling studies.
This document discusses dissolution media used for in vitro drug dissolution testing. It begins by introducing the importance of dissolution testing in assessing drug solubility, dissolution rate, and bioavailability. The key factors in selecting an appropriate dissolution medium are the drug's physicochemical properties and the purpose of the test. Common dissolution media include simulated gastric fluid (SGF), simulated intestinal fluid (SIF), water, and biorelevant media. The composition, pH, and other factors that can affect different dissolution media are also reviewed. In conclusion, the selection of the proper dissolution medium plays a crucial role in dissolution testing and developing an IVIVC for evaluating drug performance.
In this presentation I have mentioned whatever the possible relevant content required for the title.
Citation Is done at the end of slide.
Content is up to date & true to my belief.
Thanks & Best Regards.
Anurag Pandey
B.Pharm (FACULTY OF PHARMACY, INVERTIS UNIVERSITY)
M.Pharm (INSTITUTE OF PHARMACY, NIRMA UNIVERSITY)
Email :- anurag.dmk05@gmail.com
This document summarizes a seminar on using Caco-2 monolayers to study transport across the intestinal barrier. The Caco-2 cell line spontaneously differentiates into enterocytes that form a polarized monolayer mimicking intestinal absorption. Key aspects covered include Caco-2 cell culture and characterization, permeability assays to measure transport, and applications in drug development. Validation is done using reference compounds to classify drug absorption. Considerations for biological factors and analytical methods are also discussed.
Development and validation of HPLC method for the estimation of Escitalopram ...pharmaindexing
This document describes the development and validation of a HPLC method for the estimation of Escitalopram oxalate in tablets. Key points:
- An isocratic HPLC method was developed and validated for the simultaneous determination of Escitalopram in pharmaceutical formulations.
- The method used a C18 column, mobile phase of acetonitrile:methanol:ammonium acetate buffer, and UV detection at 238nm. Escitalopram had a retention time of 5.36 minutes.
- The method was validated per ICH guidelines and found to be linear, precise, accurate, rugged and robust. Analysis of tablet formulations found Escitalopram levels within 100% of claimed content.
Validated UV Method Development for the Simultaneous Estimation of Rabeprazol...pharmaindexing
This document describes the development and validation of a UV spectrophotometric method for the simultaneous estimation of Rabeprazole sodium and Cinitapride in tablet formulations. Wavelengths of 284.5 nm and 267 nm were selected for analysis of Rabeprazole sodium and Cinitapride, respectively. The method was validated according to ICH guidelines and showed good linearity, precision, and accuracy for simultaneous analysis of the two drugs without interference from excipients. The developed UV method provides a simple, accurate, and cost-effective approach for quality control testing of Rabeprazole sodium and Cinitapride in combined tablet dosage forms.
Paper electrophoresis is a technique used to separate charged molecules like amino acids, peptides, and proteins. It works by applying an electric current across a paper strip soaked in buffer solution, which causes the charged molecules in a sample to migrate across the paper at different rates based on their charge and size. The document discusses the principle, requirements, procedure, advantages, types (based on voltage and design), and factors affecting migration of molecules in paper electrophoresis. It is a simple, inexpensive, and widely-used method to qualitatively analyze biological samples for clinical diagnosis and research purposes.
Here are the key IR frequencies identified in the sample that match the reference standard of propafenone:
- C-C stretch at 1186 cm-1
- C=C stretch at 1651 cm-1
- C-H stretch (symmetric) at 2939 cm-1
- C-H bend at 1328 cm-1
- CH2 stretch (symmetric) at 1369 cm-1
- CH2 bend at 1485 cm-1
- CH3 bend at 1398 cm-1
- C-O stretch at 1100 cm-1
- C=O stretch at 1695 cm-1
- N-H stretch at 3417 cm-1
The IR spectrum
Introduction to Higuchi plots for tablet dissolution
Dissolution, Dissolution Models, Higuchi Plot
Presented by
Mohamed Omar Mahmoud
Department of Pharmaceutics
This document discusses modern pharmaceutical analytical techniques, specifically X-ray diffraction and crystallography. It defines X-ray crystallography and X-ray diffraction, explaining how crystal structures cause diffraction patterns. It describes the seven crystal systems and 14 Bravais lattices that define crystal structures. It outlines several applications of X-ray diffraction, including determining unknown crystal structures, analyzing polymers and metals, measuring particle size, and studying complexes.
This document discusses drug-excipient compatibility studies. It begins by explaining the importance of these studies in maximizing dosage form stability and avoiding formulation problems. It then describes the goals of compatibility studies and various mechanisms of drug-excipient interactions including physical, chemical, and physiological interactions. Finally, it outlines several analytical methods used in compatibility studies, including thermal techniques like DSC, spectroscopic techniques like vibrational spectroscopy, and chromatographic techniques like HPLC.
FORMULATION AND EVALUATION OF GELATIN MICROSPHERES LOADED WITH FENOFIBRATEReshma Fathima .K
The document summarizes the formulation and evaluation of gelatin microspheres loaded with the drug Fenofibrate. Two microsphere formulations were developed using a coacervation and phase separation method. Formulation F2 showed 97% drug encapsulation efficiency and released the drug over 12 hours, indicating it was suitable for oral sustained release. Evaluation tests on the microspheres showed they were spherical in shape, had good flow properties, and released the drug in a controlled manner without any burst release. The microspheres could facilitate the design of hard gelatin capsules for improved patient compliance.
Brain targeted drug delivery system seminar.pptxShamsElfalah
This document discusses brain targeted drug delivery systems and strategies to overcome the blood brain barrier (BBB). It begins with an introduction to the challenges of delivering drugs to the central nervous system (CNS) due to the BBB. It then describes the structure and function of the BBB. The rest of the document outlines various approaches to enhance drug delivery to the brain, including invasive techniques like implants and infusion, non-invasive methods like prodrugs and nanoparticles, and miscellaneous techniques. The goal is to review strategies that have been developed to help drugs cross the BBB and treat CNS disorders.
Gel chromatography, also known as gel permeation chromatography or size exclusion chromatography, separates molecules based on their size. It uses columns packed with porous beads that allow small molecules to enter the pores while excluding larger ones, resulting in smaller molecules traveling slower through the column. It is commonly used to determine the molecular weight distributions of polymers.
Dissolution is a test used to evaluate the rate of release of a drug substance from a dosage form. It assesses the performance of drug products and aims to be predictive of bioavailability. The first dissolution studies were reported in 1897 and focused on relating dissolution to drug absorption. Key factors that can affect dissolution include the apparatus used, temperature, media composition and pH, as well as formulation aspects like excipients. Proper procedure involves setting standardized conditions for the apparatus, media preparation, running the test, and calculating the percentage of drug dissolved within a specified time period.
This document discusses in-vivo methods for determining drug permeability and absorption. It describes two main types of in-vivo methods: direct and indirect. The direct method involves measuring drug levels in blood or urine over time after administration to determine the rate and extent of absorption. The indirect method measures pharmacological response instead of drug concentration when direct measurement is difficult. In-vivo methods provide a more precise understanding of factors like gastric emptying and drug effects on the gastrointestinal tract compared to in-vitro or in situ methods.
In vitro dissolution, Alternative Methods.pptxPrachi Pandey
In vitro dissolution and drug release testing provides important information about drug product performance. There are several methods for testing, including sink and non-sink methods as well as natural convection, forced convection, and continuous flow/through methods. Key factors in testing include the dissolution medium, apparatus used, sampling timepoints, and analytical methods for quantifying the amount of drug released over time. Dissolution and drug release profiles are important for understanding and comparing the in vivo behavior and therapeutic effectiveness of different drug product formulations.
ultra high performance liquid chromatographyacademic
This document provides an overview of ultra high performance liquid chromatography (UHPLC). It begins with an introduction defining UHPLC and explaining that it improves resolution, speed and sensitivity compared to traditional HPLC. The document then covers the principles of UHPLC, provides a comparison of UHPLC and HPLC parameters, describes UHPLC instrumentation including pumps, columns and detectors, and discusses applications and advantages/disadvantages of UHPLC.
Barriers to Protein and peptide drug delivery system JaskiranKaur72
Protein and peptide DDS are novel systems of drug delivery.
The successful delivery of peptide and protein-based pharmaceuticals is primarily determined by its ability to cross the various barriers presented to it in the biological milieu. Various barriers encountered are-
1 Physiological Barrier
2 Intestinal Epithelial barriers
3 Capillary Endothelial Barrier
4 Blood-Brain barrier (BBB)
The document discusses the effect of various parameters on drug dissolution. It describes how agitation, pH, viscosity, temperature and other properties of the dissolution medium influence the dissolution rate. The amount of dissolution medium and maintenance of sink conditions are also important. Various dissolution models like Hixson-Crowell are presented.
The document provides guidelines on validation of analytical procedures from the International Conference on Harmonisation (ICH) and the World Health Organization (WHO). It discusses validation characteristics like accuracy, precision, specificity, linearity, range, detection limit and quantitation limit that should be considered when validating identification tests, assays, and tests for impurities. It provides definitions for key terms and recommendations on how validation of these characteristics should be performed.
The document discusses atomic absorption spectroscopy. It begins with an introduction describing how atomic absorption spectroscopy measures the concentration of an element by measuring the amount of light absorbed at a characteristic wavelength when it passes through atoms of that element. It then describes the principle, instrumentation, applications, and sources of interference in atomic absorption spectroscopy. The key sources of interference discussed are non-spectral interferences such as matrix, chemical, and ionization interferences and spectral interferences such as background absorption.
This document discusses methods of formulating and evaluating buccal drug delivery systems. It describes the basic structure and designs of buccal dosage forms as being matrix or reservoir types. The key components are outlined as the drug substance, bioadhesive polymers, backing membrane, and permeation enhancers. Various formulation methods are provided for solid, semi-solid and liquid buccal dosage forms including tablets, patches, films, gels and sprays. Evaluation methods are also summarized such as weight variation, thickness, friability, hardness, and in-vitro swelling studies.
This document discusses dissolution media used for in vitro drug dissolution testing. It begins by introducing the importance of dissolution testing in assessing drug solubility, dissolution rate, and bioavailability. The key factors in selecting an appropriate dissolution medium are the drug's physicochemical properties and the purpose of the test. Common dissolution media include simulated gastric fluid (SGF), simulated intestinal fluid (SIF), water, and biorelevant media. The composition, pH, and other factors that can affect different dissolution media are also reviewed. In conclusion, the selection of the proper dissolution medium plays a crucial role in dissolution testing and developing an IVIVC for evaluating drug performance.
In this presentation I have mentioned whatever the possible relevant content required for the title.
Citation Is done at the end of slide.
Content is up to date & true to my belief.
Thanks & Best Regards.
Anurag Pandey
B.Pharm (FACULTY OF PHARMACY, INVERTIS UNIVERSITY)
M.Pharm (INSTITUTE OF PHARMACY, NIRMA UNIVERSITY)
Email :- anurag.dmk05@gmail.com
This document summarizes a seminar on using Caco-2 monolayers to study transport across the intestinal barrier. The Caco-2 cell line spontaneously differentiates into enterocytes that form a polarized monolayer mimicking intestinal absorption. Key aspects covered include Caco-2 cell culture and characterization, permeability assays to measure transport, and applications in drug development. Validation is done using reference compounds to classify drug absorption. Considerations for biological factors and analytical methods are also discussed.
Development and validation of HPLC method for the estimation of Escitalopram ...pharmaindexing
This document describes the development and validation of a HPLC method for the estimation of Escitalopram oxalate in tablets. Key points:
- An isocratic HPLC method was developed and validated for the simultaneous determination of Escitalopram in pharmaceutical formulations.
- The method used a C18 column, mobile phase of acetonitrile:methanol:ammonium acetate buffer, and UV detection at 238nm. Escitalopram had a retention time of 5.36 minutes.
- The method was validated per ICH guidelines and found to be linear, precise, accurate, rugged and robust. Analysis of tablet formulations found Escitalopram levels within 100% of claimed content.
Validated UV Method Development for the Simultaneous Estimation of Rabeprazol...pharmaindexing
This document describes the development and validation of a UV spectrophotometric method for the simultaneous estimation of Rabeprazole sodium and Cinitapride in tablet formulations. Wavelengths of 284.5 nm and 267 nm were selected for analysis of Rabeprazole sodium and Cinitapride, respectively. The method was validated according to ICH guidelines and showed good linearity, precision, and accuracy for simultaneous analysis of the two drugs without interference from excipients. The developed UV method provides a simple, accurate, and cost-effective approach for quality control testing of Rabeprazole sodium and Cinitapride in combined tablet dosage forms.
Analysis of analgesics and antipyretics.induhdghcfgfgftf
The document summarizes analytical methods for several analgesics and antipyretics. It discusses classification of analgesics and antipyretics and their mechanisms of action. Specific analytical methods covered include titrimetric, spectrophotometric, chromatographic and colorimetric assays for drugs like aspirin, diclofenac sodium, aceclofenac, ibuprofen, paracetamol, analgin and antipyrine. Gravimetric, colorimetric and polarographic methods are described for the analysis of antipyrine.
This document discusses toxicity testing of chemicals in marine environments. It defines aquatic toxicity, degradability, and bioaccumulation as key properties to assess the potential hazards of chemicals. The goal of toxicity tests is to determine the concentration of a chemical that causes unacceptable negative effects. Toxicity data from species in three trophic levels (algae, invertebrates, fish) is used to calculate the predicted no-effect concentration (PNEC). The document outlines how to determine toxicity through dose-response relationships and endpoints like median lethal concentrations and no observed effect concentrations.
This document provides an overview of toxicity testing methods for acute, subacute, and chronic toxicity studies. It discusses the importance and history of toxicity testing, as well as standard methods and guidelines established by organizations like OECD and EPA. A variety of in vivo and in vitro toxicity tests are described, including acute, repeated dose, genotoxicity, carcinogenicity, and local toxicity studies. The document also addresses the large number of animals used annually for toxicity testing globally and the regulatory framework for animal testing in India.
RP-HPLC METHOD FOR THE QUANTIFICATION OF OXALIPLATIN IN FORMULATIONSIJSIT Editor
The document describes the development and validation of an RP-HPLC method for the quantification of oxaliplatin in formulations. The method utilizes a C18 column with a mobile phase of methanol, acetonitrile and water at a flow rate of 1 mL/min. Oxaliplatin was detected at 240nm with a retention time of 8.33 minutes. The method demonstrated good linearity, precision, accuracy and can be used for routine analysis of oxaliplatin in tablets and bulk drug.
A novel validated RP-HPLC method was developed for the estimation of Liraglutide in bulk and parenteral dosage forms. The method utilized a C18 column, mobile phase of methanol and phosphate buffer with UV detection at 246 nm. The method was validated per ICH guidelines and found to be accurate, precise, specific and linear over a concentration range of 20-80 μg/ml. The method was successfully applied to a liraglutide injection sample with 99.84% assay results. In summary, a novel HPLC method was developed and validated for the analysis of liraglutide in bulk and parenteral dosage forms.
Traditional Kashmiri Recipe “Shangri-Kahwa” as a Stimulant Drink and Effectiv...SriramNagarajan15
The popular recipe “Shangri-kahwa” is an age old home remedy for respiratory and various other problems in almost whole of Kashmir. It is prepared from important spices like liquorice, clove, cinnamon, and cardamom, which have documented health benefits. Information about its use and method of preparation was obtained from group discussions held in some villages of Baramullah district of Jammu and Kashmir. People in these villages believe that Shangri-kahwa is cost effective, delicious, made from easily available ingredients and can be prepared easily at home. Being residents of this area, the authors are aware of the popularity of this magical drink used as a first line of treatment for various ailments at home, particularly during cold days. This recipe is extremely famous in these villages both as a refreshing and stimulant drink, as well as believed to be highly efficacious in respiratory illnesses. It is cost effective and highly palatable. The ingredients of Shangri-kahwa are being used extensively in Unani system of medicine and Ayurveda for almost same indications as the recipe is used. This study was carried out to highlight the effectiveness and focus the attention of the researchers towards this attractive and effective dosage form used as home remedy in Kashmir.
Spectrophotometric Estimation of Rosuvastatin Calcium in Bulk and Pharmaceuti...pharmaindexing
This document describes the development and validation of an RP-HPLC method for the simultaneous estimation of Lamivudine and Zidovudine in tablet dosage forms. The method utilizes a C18 column with a mobile phase of ammonium acetate buffer pH 4.0, acetonitrile and THF in a 60:30:10 ratio at a flow rate of 1 mL/min. Lamivudine and Zidovudine were well separated with retention times of 3.793 minutes and 2.547 minutes, respectively. The method was validated per ICH guidelines and demonstrated to be linear, precise, accurate, specific and robust for the simultaneous analysis of these two drugs in tablets.
Determination of Etodolac in Commercial Formulations by HPLC-UV Methodijtsrd
The aim of this study was to develop and verify a simple, rapid and sensitive high performance liquid chromatography method coupled with UV detector HPLC UV method for the quantitative determination of etodolac in bulk and pharmaceutical dosage forms. Chromatographic separation was performed at ambient conditions on a reverse phase ACE C8 analytical column 250 mm x 4.6 mm ID, 5 umm using the mobile phase containing acetonitrile water 80 20, v v at a flow rate of 1.0 mL min 1. A wavelength of 272 nm was used for etodolok and paracetamol IS . A retention time of 4.21 min and 2.02 min were obtained for etodolac and IS, respectively. The method showed linearity in the range of 0.08 10 µg mL 1 for etodolac R = 0.9999 . The linear regression equations obtained by least square regression method were the ratio of peak area of etodolac and IS =1.559 concentration etodolac µg mL 0.139. The intra day and inter day RE and RSD values of the method were =10.0 and =2.65 , respectively. Limit of detection LOD and limit of quantification LOQ were found to be 0.04 and 0.06 µg mL 1 for etodolac, respectively. A new, simple and sensitive high performance liquid chromatography method was developed and validated for etodolac. The method can be applied for the quantification of etodolac without derivatization in bulk solutions and commercial formulations using the internal standard. Tugrul Cagri Akman | Yucel Kadioglu "Determination of Etodolac in Commercial Formulations by HPLC-UV Method" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29452.pdfPaper URL: https://www.ijtsrd.com/pharmacy/analytical-chemistry/29452/determination-of-etodolac-in-commercial-formulations-by-hplc-uv-method/tugrul-cagri-akman
RP-HPLC Assay Method Validation for the estimation of new Anti-retroviral dru...SriramNagarajan15
A reverse phase HPLC method was developed and validated for the quantification of lamivudine in bulk and tablet formulations. The method used an ODS column with a mobile phase of acetate buffer and acetonitrile (50:50) at a flow rate of 1.5 mL/min. Lamivudine had a retention time of 1.85 minutes when detected at 272 nm. The method was linear over a concentration range of 10-50 μg/mL with a correlation coefficient of 0.999. Accuracy and precision studies demonstrated recoveries between 98-102% and %RSD below 2%, respectively. The method was found to be robust, specific, and suitable for the routine analysis of lamivudine in
The document describes the development and validation of a reverse phase HPLC method for the estimation of the anti-retroviral drug lamivudine in bulk and tablet formulations. An ODS column with a mobile phase of acetate buffer and acetonitrile was used to achieve separation of lamivudine. The method was validated as per ICH guidelines and was found to be linear, precise, accurate and robust. The developed method can be used for the routine analysis of lamivudine in pharmaceutical dosage forms.
RP-HPLC Assay Method Validation for the estimation of new Anti-retroviral dru...SriramNagarajan15
A Reverse phase HPLC method was developed for estimation of the Lamivudine in bulk and tablet formulation by using ODS column (250mm×4.6mm, 5µm) and Acetate buffer: acetonitrile (50:50) as mobile phase, at a flow rate of 1.5ml/min. The detection was carried at the 272nm the retention time of the Lamivudine is 1.850. The developed method was validated for the various parameters as per the ICH guidelines like accuracy precision, linearity and range, Robustnes. Linearity was obtained in the concentration range of 10µg/ml to 50µg/ml with correlation coefficient of 0.999. The accuracy of the method was assessed by recovery studies at three different concentration levels. The percentage recovery of Lamivudine was found to be in the range of 98% -102%. The method was found to be precise as indicated by the repeatability, inter-day, intra-day analysis, showing %RSD less than 2. Key words: RP-HPLC, Lamivudine, Pharmaceutical dosage form.
The document describes the development and validation of a reverse phase HPLC method for the estimation of the anti-retroviral drug lamivudine in bulk and tablet formulations. An ODS column with a mobile phase of acetate buffer and acetonitrile was used to achieve separation of lamivudine. The method was validated as per ICH guidelines and was found to be linear, precise, accurate and robust. The developed method can be used for the routine analysis of lamivudine in pharmaceutical dosage forms.
Development and validation of new rp hplc method for analysis of capecitabine...IJSIT Editor
A new precise accurate and reliable validated method for the determination of Capecitabine has been
developed by using reverse phase high performance liquid chromatography in pharmaceutical dosage forms.
Spectrophotometric determination was carried out at an absorption maximum of 230nm by using methanol.
The linearity was over the concentration range of 20-120 μg/ml with correlation coefficient 0.999.
Chromatographic separation was carried out by using a mobile phase of methanol: Acetonitrile: water
(80:18:2 V/V) on Zodiac C18 column (250 mm X 4.6 mm, 5 μm) in an isocratic mode at a flow rate of 1.1
ml/min with UV detection at 230 nm. The developed methods were found to be precise and accurate for the
estimation of Capecitabine in pharmaceutical dosage forms and could be used for routine analysis.
Development and Validation of Reverse Phase Liquid Chromatography Method for ...IOSR Journals
This document describes the development and validation of a reverse phase liquid chromatography method for the estimation of losartan in bulk drug samples. The method utilizes an Acquity BEH C18 column with a mobile phase of buffer and acetonitrile at a ratio of 50:50 delivered isocratically at 0.3 mL/min. Losartan was detected at 230 nm. The method was validated per ICH guidelines and found to be linear, precise, accurate, specific and stability-indicating for the quantification of losartan in the range of 25-75 μg/mL. The method validation shows the method is suitable for the routine analysis of losartan in bulk drug materials.
Development and validation of hplc method for determination of theophylline a...IJSIT Editor
A stable, simple, rapid, precise, accurate HPLC method for analysis of Theophyllinee and 1-Methyl
Uric Acid was developed and validated as per ICH guidelines without need of any internal standard.
Separation was carried out using X’terra RP18 (250*4.6) mm, 5µ column with potassium dihydrogen
orthophosphate buffer (pH 3): acetonitrile (30:70 v/v) as mobile phase with flow rate 1 mL min-1. The
parameters studied were retention time, linearity and range, accuracy, precision. The proposed method can
be used for determination of Theophylline and 1-Methyl Uric Acid from Human plasma.
Development and Validation of the HPLC Method for the Analysis of Ametridio...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Application of Validated High-performance Liquid Chromatography Method for De...BRNSS Publication Hub
A novel and simple reversed-phase liquid chromatographic method has been established for the determination of saxagliptin and metformin HCl Saxagliptin and metformin HCl is used to control Type 2 diabetes. The proposed work was performed on Young Lin (S.K) isocratic System UV Detector. Saxagliptin and metformin HCl is used to control Type 2 diabetes. The proposed work was performed on Young Lin (S.K) isocratic System UV Detector C18 column (150 mm × 4.6 mm). A mixture of potassium phosphate, mobile phase in this method with flow rate of 0.7 mL/min (UV detection at 203 nm) and the method was validated as per the ICH guidelines. Forced degradation studies were performed by exposing the drug saxagliptin and metformin HCl to acidic, alkaline, oxidation, and thermal stress degradations. The proposed reversed-phase-high-performance liquid chromatography method was found to be robust and specific, and this method is suitable for the assay of pharmaceutical dosage forms as well as kinetic studies.
This document describes the development and validation of an RP-HPLC method for the simultaneous estimation of atorvastatin and olmesartan tablets. The method was developed using a BDS hypersil C18 column with a mobile phase of ACN-TEA 40-60-0.1 pH 4.0 and detection at 235 nm. The method was validated for parameters such as linearity, precision, accuracy, robustness and system suitability and found to be suitable for the simultaneous quantification of atorvastatin and olmesartan in combined tablet dosage forms.
IOSRPHR(www.iosrphr.org) IOSR Journal of Pharmacyiosrphr_editor
A simple reverse phase liquid chromatographic method was developed and validated for the simultaneous estimation of lornoxicam and paracetamol from their pharmaceutical dosage forms. The method utilized a C18 column with a mobile phase of potassium dihydrogen phosphate (pH 7.3) and acetonitrile (70:30) and detected compounds at 257nm. The method was linear over 20-60μg/ml for paracetamol and 0.2-1.8μg/ml for lornoxicam. Retention times were 2.33 minutes for paracetamol and 7.61 minutes for lornoxicam. The method was validated per ICH guidelines and demonstrated good precision, accuracy, reproducibility
IOSRPHR(www.iosrphr.org) IOSR Journal of Pharmacyiosrphr_editor
A simple reverse phase liquid chromatographic method was developed and validated for the simultaneous estimation of lornoxicam and paracetamol from their pharmaceutical dosage forms. The method utilized a C18 column with a mobile phase of potassium dihydrogen phosphate (pH 7.3) and acetonitrile (70:30) and detected compounds at 257nm. The method was linear over 20-60μg/ml for paracetamol and 0.2-1.8μg/ml for lornoxicam. Retention times were 2.33 minutes for paracetamol and 7.61 minutes for lornoxicam. The method was validated per ICH guidelines and demonstrated good precision, accuracy, reproducibility
CHITINASE AS THE MOST IMPORTANT SECONDARY METABOLITES OF STREPTOMYCES BACTERISIJSIT Editor
This document summarizes a study on chitinase produced by Streptomyces bacteria. 310 Streptomyces isolates were obtained from soil samples collected across East Azerbaijan, Iran. DNA was extracted from the isolates and screened using PCR for a gene encoding family 19 chitinase, which shows significant antifungal activity. One isolate with strong antifungal effects was identified as S. griseous based on 16S rDNA sequencing and presence of the chitinase gene. The results suggest certain Streptomyces isolates have potential as biological control agents against fungal plant pathogens due to production of antifungal family 19 chitinase.
MOLECULAR ANALYSIS OF BACTERIAL GENE CODING CHITINASE ENZYMES, FAMILY 19 STR...IJSIT Editor
The document summarizes a study on molecular analysis of bacterial genes coding for chitinase enzymes in Streptomyces bacteria. Streptomyces are known to produce chitinase enzymes that belong to families 18 and 19 and have antifungal activity. The study isolated 310 Streptomyces bacteria from soil samples in East Azerbaijan, Iran. DNA was extracted from the isolates and PCR was performed to identify isolates containing genes for family 19 chitinases. Five isolates tested positive and one showed strong antifungal activity. The gene from this isolate was cloned and sequencing identified it as a family 19 chitinase gene with 96% similarity to Streptomyces griseus. The isolate shows potential as a
THE EFFECTS OF HELPING BACTERIA (PSEUDOMONAS SPP.) IN NITROGEN GREEN BEANS F...IJSIT Editor
Some- bacteria settle in the rhizosphere of legume plants and enhance the performance of ribosome
bacteria to nitrogen fixation and nodulation. In this paper, we used four isolated from two species of
Pseudomonas containing P.putida, P.fluorescens Chao, P.Flouresence Tabriz, P.flouresence B119 and Rhizobium
leguminosarumbv.phaseoli. In a factorial experiment with complete randomized blocks were used 5 levels of
helping bacteria(Pseudomonas spp.) and two rhizobium levels, four replicates were employed. Jamaran418
green bean was utilized as host plant. At the end, nodulation, growth and plant’s nitrogen indexes were
measured. The results showed that all above mentioned helping bacteria enhance the growth and nodulation
performance of green bean. It should be said that P.putida had the highest effect on the green bean
nodulation increase along with rhizobium (130%) followed by P.fluorescens Tabriz, P. fluorescens Chao and
P.fluorescens B119, ( 83, 63 and 17%, respectively). Also, we observed 45, 33, 22 and 8% performance
increase under the effect of P.putida, P. fluorescens Chao, P. fluorescens Tabriz and P. fluorescens B119,
respectively.
ANTIMICROBIAL PROPERTY OF AQUEOUS AND PETROLEUM ETHER LEAF EXTRACTS OF JATRO...IJSIT Editor
The experiment was carried out to investigate the antimicrobial property of aqueous and Petroleum
ether leaf extracts of Jatrophacurcas against some gram positive micro-organisms: Staphylococcus aureus,
Bacillus subtilis and some gram negative micro-organisms: Escherichia coli, Salmonella typhi using
antibiotics; Gentamycin as control. The phytochemical screening of aqueous and petroleum ether extracts
showed the presences of cardiac glycosides, steroids and terpenes, tannins, phlobatannins, anthraguinones
and saponins. The disc diffusion techniques was used to test the sensitivity of the micro-organism to the
extracts of Jatrophacurcas the results obtained show mean zones of inhibition between (19 + 0.6mm) to (30 +
0.3mm) for aqueous extract and (24 + 0.5mm) to (35 + 0.8mm) for petroleum ether extract. Micro-organisms
showed sensitivity in the following order: E.coli;(17 + 0.3mm) and (25 + 0.8mm), S.aureus; (26 + 0.2mm) and
(28 + 0.6mm), B.subtilis; (16 + 0.1mm) and (20 + 0.7mm), and S.typhi (25 + 0.2mm) and (27 + 0.6mm) for
aqueous and petroleum ether extracts respectively. The minimum inhibition concentration (MIC) for both
extracts show that the extracts inhibited the growth of the entire test organism at concentration 0.6mg/ml.
This result thus suggests the potency of Jatrophacurcas as an antimicrobial agent especially at the
concentration employed.
BIO CHEMICAL EFFECT OF 1, 5-BIS (3, 5-DIMETHYLPYRAZOL-1-YL)-3- OXAPENTANE-DIA...IJSIT Editor
The document summarizes a study that investigated the biochemical effects of 1,5-Bis(3,5-Dimethylpyrazol-1-yl)-3-oxapentane-diacetatocopper in albino rats. The study found that the compound had antidiabetic effects by lowering blood glucose levels but also caused abnormalities. Rats treated with the compound showed decreases in serum glucose and albumin levels but increases in ALT and AST levels. Long-term treatment for 6 weeks also significantly decreased body weight in treated rats. The compound affected both liver and blood biochemistry in rats.
THE EFFECT OF ALSTONEA BOONEI STEM BARK PLUS CISPLATININDUCED RENAL INSUFFIC...IJSIT Editor
The bark of Alstoniaboonei stem was analysed for the medicinal and the effect of extracts on induced
renal insufficiency. The plant material was collected in August-September 2012 and Rats 100-150g body
weights were subjected to the study. Normal saline as control, Cisplatin, and cisplatin plus Alstoneiboonei
stem bark extract were administered and the result summary for serum creatinine in cisplatin treated Rats
(2.69±0.32mg/dl) and in Rats administrates cisplastin plus Alstoniaboonei stem bark extract
(2.5±0.01mg/dl) were elevated compared to saline control (1.89±0.89mg/dl). Serum urea in cisplatin treated
Rats was (38.4 ±2.98mg/dl) compared to Rats administrates with cisplatin plus the extract (38.4±2.98mg/dl)
and saline control (24.94±3.76mg/dl). The study indicates Alstoniaboonei stem bark extract reduced the
renal insufficiency in rats.
The study was carried out to investigate the effect of the aqueous extracts of
Myristicafragrans(Nutmeg), Murrayakoenigi(curry leaf) and Aframomummelegueta(Guinea pepper) on Some
Biochemical and haematologicalParameters. Sixteen (16) wister strain rats weighing between 130 – 180g
were divided into four (4) groups of four (4) rats each and for 21 days fed the following diets: Group A –
normal diet + myristicafragrans (Nutmeg) aqueous extract, Group B – normal diet + murrayakoenigi (curry
leaf) aqueous extract, Group C – normal diet + aframomummelegueta (Guinea pepper) aqueous extract, Group
D – normal diet (control). After a period of 21 days the rats were sacrificed and the serum was taken for the
following estimations: total protein, albumin, total bilirubin, direct bilirubin, aspartate transaminase, alanine
transaminase, alkaline phosphatase, total cholesterol, triglyceride, HDL cholesterol, LDL cholesterol and
glucose. The whole blood was taken for packed cell volume and white blood cell count. The results indicated
that oral administration of myristicafragrans, murrayakoenigi and aframomummelegueta to rat’s exhibit
remarkable hypolipidaemic activity and lowering glucose concentration. The oral administration of these
three spices exhibit protein increasing activities compared with the control rats. The packed cell volume and
white cell values of all the rats decreased after feeding with experimental diet (aqueous extract) compare
with the control rats. It is clear from this study thatMyristicafragrans(Nutmeg), Murrayakoenigi(curry leaf)
andAframomummelegueta (Guinea pepper) contain significant amounts of phytochemicals and exhibit
hypolipidaemic activity when consumed.
THE INFLUENCE OF SILICONE ANTIFOAM FROM LEATHER AND DYING WASTE WATER EFFLUE...IJSIT Editor
This study investigates the influence of silicone antifoam agent on waste water from Gashash leather
and Nigerian Spinning and Dying industries (NSD). Waste water from the outlet of the industries were
collected and analyzed for physicochemical parameters. Silicone antifoam was added to the wastewater to
determine the impact of the silicone antifoam on turbidity and chemical oxygen demand (COD)
concentrations. The result shows that both turbidity and COD values significantly increased even when small
concentration of the silicone antifoam was added. Further, independent t-test was used to identify the
variance between the mean value of the wastewater from leather, spinning and dying industries, the results
indicated that there are no significant differences (observed t 0.544, critical t 2.015, and p value 0.589)
between the waste water in leather and dying industries.
WATER INTAKE CHARACTERISTICS OF DIFFERENT SOIL TYPES IN SOUTHERN BORNO NIGERIA IJSIT Editor
The water intake characteristics of soils under arable crop practice were studied with a view to
obtaining useful information for the design of irrigation and drainage system and for effective soil
management techniques. Parameters determined; infiltration, hydraulic conductivity, permeability, bulk
density, particle density, porosity and moisture content. The textural class of the soils from the three sites
was found to be clay. The result obtained indicates that infiltration was high initially but decreases later. This
may be due to the soil reaching a saturation point. On the average the infiltration rate was observed to
decrease with time. The coefficient of permeability was found to be 9.26 x 10 , 7.66 x 10 and 2.15 x 10 cm/s
for site A, B and C respectively. Information on infiltration and permeability are useful tools in irrigation and
other engineering design.
DETERMINATION OF ENGINEERING PROPERTIES OF POMEGRANATE FRUIT TO CALCULATION ...IJSIT Editor
In avoiding damage to fruit species the permissible falling height and permissible static pressure are
of great importance. The former is important in planning harvesting and handling operations, the latter in
selecting the height of transport containers. Fruits are generally transported in containers. The static and
dynamic forces which then act on the fruit will cause damage if they exceed given value. The static force may
be calculated from the weight of the fruit column being transported while the dynamic load is a consequence
of vibration caused by transport. The permitted static load for a given fruit may be determined
experimentally. In this study, physical properties of interest were determined for fresh pomegranate fruit
then calculations for the design of a suitable height were conducted based on the measured properties using
Ross and Isaacs’s theory. Maximum height for packing and storing of fresh pomegranate fruit in the box was
determined to be less than 123 cm based on a rupture force of 40.7 N.
COMPARSION OF ANTIOXIDANT POTENTIAL OF DIMOCARPUS LONGAN LOUR. EXTRACTS AND ...IJSIT Editor
The present study was carried out to evaluate antioxidant activity of Dimocarpus longan stems
extracts and also to investigate the main phytoconstituents in the bio-active extract. N-hexane,
dichloromethane, ethyl acetate and methanol 80% extract were tested for free radical scavenging activity on
model reaction with stable 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). The results showed that ethyl
acetate was the most active one as antioxidant agent and phytochemical analysis of that extract revealed the
presence of triterpenes, flavonoids, tannins and carbohydrates. The results may help to discover new
chemical classes of natural antioxidant substances that could serve as selective agents for infectious diseases.
DIRECT EXPANSION GROUND SOURCE HEAT PUMPS FOR HEATING AND COOLINGIJSIT Editor
This article is an introduction to the energy problem and the possible saving that can be achieved
through improving building performance and the use of ground energy sources. The relevance and
importance of the study is discussed in the paper, which, also, highlights the objectives of the study, and the
scope of the theme. This study discusses some of the current activity in the GSHPs field. The basic system and
several variations for buildings are presented along with examples of systems in operation. Finally, the GCHP
is presented as an alternative that is able to counter much of the criticism leveled by the natural gas industry
toward conventional heat pumps. Several advantages and disadvantages are listed. Operating and installation
costs are briefly discussed.
BIOMINERALISED SILICA-NANOPARTICLES DETECTION FROM MARINE DIATOM CULTURE MEDIAIJSIT Editor
Diatoms are unicellular algae the most spectacular among the microorganisms assemble into a
micro-shell with a distinct 3-D shape and pattern of fine nanoscale features. In this investigation, we present
results; Field Emission Scanning Electron Microscopy images show the presence of ordered arrays of silica
nanoparticles. A number of diatoms with partially opened valves were observed on the surface of the diatom,
which indicates that cell contents inside of diatoms could release the nanoparticles into the culture solution.
We believe that the film forming silica nanoparticles are either released by the diatoms during reproduction
or after cell death due to bacterial action. Further research will investigate whether the silica nanoparticles
are produced intracellular and then released or whether synthesis occurs in cell culture medium. This
approach provides an environmentally friendly means for fabricating silica nanoparticles for drug delivery,
disease diagnostics, artificial opal films, decorative coatings and novel optical materials.
COMPARATIVE STUDIES ON NUTRITIONAL VALUE OF NORMAL AND TUMOR TISSUE, SARDINE...IJSIT Editor
This document summarizes a study on the nutritional value of normal and tumor tissue in Sardinella longiceps fish from India. The study found that protein levels were higher in normal tissue (29.15%) compared to tumor tissue (18.93%). Fatty acid analysis revealed higher levels of polyunsaturated fatty acids like linolenic acid in normal tissue. Vitamin A levels were also higher in normal tissue. The study analyzed minerals, amino acids, fatty acids, vitamins and other biochemical components to determine the nutritional composition and value of S. longiceps fish tissue. The results show that S. longiceps is a valuable food source for humans due to its high quality protein and balanced nutritional profile.
ANTIFUNGAL ACTIVITY OF SELECTED MEDICINAL PLANT EXTRACS AGAINST PLANT PATHOG...IJSIT Editor
The aim of this work was to find an alternative to chemical fungicides currently used in the control
plant pathogenic fungi Rhizoctoniasolani ,ColletotrichummusaeandFusariumoxysporum,. The antifungal
activity of the methanol extracts of six medicinal plants used in native medicine in Sri Lanka is reported.All
plant extracts were screened for their fungistatic, fungicidal activities and minimum inhibitory dilution (MID)
against above fungi. The media amended with methanol and recommended fungicide for respective fungal
strain were consider as negative and positive control respectively.Results showed that radial growth in all the
three tested organisms was significantly impaired (p<0.05) by the addition of the extracts in the culture
medium used. The test fungi differed in their reaction to the different extracts but on the whole, growth
inhibition increased with the concentration of each extract. The most active extracts, shows a marked effect of
the 20% methanol extracts from sweet flag with inhibition values of 91%, 86% and 84 % for F. oxysporum,R.
solani and C.muceawhereas those from wild basil inhibited the growth of the same pathogens by 89%, 84%
and 74%.The results showed minimal inhibitory concentrations (MIC) were 5 % (v/v) for sweet flag and wild
basil and 20% (v/v) for all other plant crude extracts. Out of six plants extract screened, wild basil and sweet
flag showed more than 80% fungal inhibition after 6 hour immersion and other extracts could not exceed
60% inhibition after any exposure time. The study revealed that methanol crude extract of sweet flag and
wild basil exhibit strong fungistatic and fungicidal activities against tested fungi. These results support the
potential use of these plant extracts in the management of diseases caused by tested plant pathogenic fungi.
OUTCOME OF TUNNELED CATHETERS IN HEMODIALYSIS PATIENTS: FIVE YEARS SINGLE CE...IJSIT Editor
Introduction: The tunneled hemodialysis catheters(THCs) are preferred for the patients who are expected to
poor survival and the attempts to arteriovenous fistulas (AVF) are failure. In our study,in hemodialysis
patients who are implemented tunneled catheter it is evaluated the mean duration for the catheters , their
complications and the factors which affect the period of the catheters.
Methods: At the Antalya Research and Education Center Hemodialysis Unit it is retrospectively evaluated the
data of 297 hemodialysis patients who are implemented tunneled catheter during 5 years .
Results: The mean duration time of the tunneled catheters has been 224.9+162.9 days. The duration time of
right internal jugular vein(RIJV) is considerably higher than left internal jugular vein(LIJV) and subclavian
veins (235.8+96.6 days). In diabetic hemodialysis patients, the duration time of the catheter is rather lower
than the other end stage renal disease reasons(184.4±72.1 days).
Conclusions: THCs must be considered as an alternative but not a permanent vascular access in hemodialysis
patients. Because of relatively short duration times than AVF, high infection risks and thrombosis , it must be
used only in patients who have problems with the creating permanent vascular access or patients with
expected low survival time. Moreover, it must be taken into consideration the duration time of the catheter is
low in diabetic hemodialysis patients. According to our results, catheter duration time was longer in RIJV than
in other insertion sites and RIJV must be preferred as first place to placement of THCs.
ANTIBACTERIAL ACTIVITY OF Citrus limonON Acnevulgaris (PIMPLES) IJSIT Editor
This document summarizes research on the antibacterial activity of lemon juice (Citrus limon) on Propionibacterium acnes, the bacteria that causes acne vulgaris (pimples). Samples were collected from patients and tested against lemon juice and a conventional cleanser at various concentrations. Both lemon juice and the cleanser were found to inhibit the bacteria, with lemon juice showing stronger antibacterial effects at lower concentrations. The minimum inhibitory concentration of lemon juice was found to be 60%, compared to 60% for the cleanser. At concentrations of 80% or higher, both lemon juice and the cleanser showed bactericidal effects, completely killing the bacteria. The study concludes that lemon juice is more effective than conventional clean
COMPARATIVE STUDY ON HEAVY METAL CHARACTERISTICS OF LEACHATE FROM MUNICIPAL ...IJSIT Editor
Rapid urbanization and population growth are largely responsible for very high increasing rate of
solid waste in the urban areas, its proper management and recycling is major problems of Municipal
Corporation. The analytical analysis revealed that the leachate show high concentration of heavy metals viz.,
Pb, Zn, Fe, Mn and Cu. However, their high concentration in municipal solid waste leachate may cause
contaminants for environmental pollution. Therefore, present investigation deals with analyze the heavy
metals concentration in municipal solid waste leachate.
PHARMACOGNOSTICAL AND PHYTO–CHEMICAL EVALUATION OF RAKTADUSHTIHAR YOGAIJSIT Editor
The Rakta has vital role in the maintenance of health. If Rakta is in proper quantity and having desirable
qualities too, it promotes health, improves complexion, strength and vigor. Raktadushtihara Yoga was
formulated to assess its role in the management of Raktadushti. The present study deals with the
standardization of Raktadushtihara Yoga through the Pharmacognostical and pharmaceutical standards.
Organoleptic features of coarse powder were within normal range. The pH value was 6.5, water soluble
extract 46.9% w/w, methanol soluble extract 25.9%, ash value 8.73%, loss on drying 9.63% and average
weight was 512 mg. HPTLC was carried out after organizing appropriate solvent system in which maximum 2
spots were distinguished at 254 nm.
Chapter wise All Notes of First year Basic Civil Engineering.pptxDenish Jangid
Chapter wise All Notes of First year Basic Civil Engineering
Syllabus
Chapter-1
Introduction to objective, scope and outcome the subject
Chapter 2
Introduction: Scope and Specialization of Civil Engineering, Role of civil Engineer in Society, Impact of infrastructural development on economy of country.
Chapter 3
Surveying: Object Principles & Types of Surveying; Site Plans, Plans & Maps; Scales & Unit of different Measurements.
Linear Measurements: Instruments used. Linear Measurement by Tape, Ranging out Survey Lines and overcoming Obstructions; Measurements on sloping ground; Tape corrections, conventional symbols. Angular Measurements: Instruments used; Introduction to Compass Surveying, Bearings and Longitude & Latitude of a Line, Introduction to total station.
Levelling: Instrument used Object of levelling, Methods of levelling in brief, and Contour maps.
Chapter 4
Buildings: Selection of site for Buildings, Layout of Building Plan, Types of buildings, Plinth area, carpet area, floor space index, Introduction to building byelaws, concept of sun light & ventilation. Components of Buildings & their functions, Basic concept of R.C.C., Introduction to types of foundation
Chapter 5
Transportation: Introduction to Transportation Engineering; Traffic and Road Safety: Types and Characteristics of Various Modes of Transportation; Various Road Traffic Signs, Causes of Accidents and Road Safety Measures.
Chapter 6
Environmental Engineering: Environmental Pollution, Environmental Acts and Regulations, Functional Concepts of Ecology, Basics of Species, Biodiversity, Ecosystem, Hydrological Cycle; Chemical Cycles: Carbon, Nitrogen & Phosphorus; Energy Flow in Ecosystems.
Water Pollution: Water Quality standards, Introduction to Treatment & Disposal of Waste Water. Reuse and Saving of Water, Rain Water Harvesting. Solid Waste Management: Classification of Solid Waste, Collection, Transportation and Disposal of Solid. Recycling of Solid Waste: Energy Recovery, Sanitary Landfill, On-Site Sanitation. Air & Noise Pollution: Primary and Secondary air pollutants, Harmful effects of Air Pollution, Control of Air Pollution. . Noise Pollution Harmful Effects of noise pollution, control of noise pollution, Global warming & Climate Change, Ozone depletion, Greenhouse effect
Text Books:
1. Palancharmy, Basic Civil Engineering, McGraw Hill publishers.
2. Satheesh Gopi, Basic Civil Engineering, Pearson Publishers.
3. Ketki Rangwala Dalal, Essentials of Civil Engineering, Charotar Publishing House.
4. BCP, Surveying volume 1
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VALIDATED RP - HPLC METHOD FOR THE ESTIMATION OF LIRAGLUTIDE IN TABLET DOSAGE
1. Alavala Siva Madhavi et al., IJSIT, 2012, 1(1), 17-26
VALIDATED RP - HPLC METHOD FOR THE ESTIMATION OF LIRAGLUTIDE
IN TABLET DOSAGE
P.V.V. Satyanarayana*, Alavala Siva Madhavi
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
ABSTRACT
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for
rapid assay of Liraglutide in tablet dosage form. Isocratic elution at a flow rate of 1.0ml/min was employed on
a symmetry C18 (250x4.6mm, 5µm in particle size) at ambient temperature. The mobile phase consisted of
0.1% Ortho Phosphoric Acid: Acetonitrile: Methanol 5:60:35 (V/V). The UV detection wavelength was 245nm
and 20µl sample was injected. The retention time for Liraglutide was 4.447 min. The percentage RSD for
precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH
guidelines. The method was successfully applied for routine analysis of Liraglutide in tablet dosage form.
Key Words: Liraglutide, RP-HPLC, UV detection, recovery, precise.
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2. Alavala Siva Madhavi et al., IJSIT, 2012, 1(1), 17-26
INTRODUCTION
Liraglutide is a long-acting glucagon-like peptide-1 (GLP-1) analog that has been developed by Novo
Nordisk for the treatment of type2 diabetes. The product was approved by the European Medicines
Agency (EMA) on July 3, 2009, and by the U.S. Food and Drug Administration (FDA) on January 25, 2010.
Figure 1: Stricture of Liraglutide
Liraglutide intended to help lower blood sugar levels along with diet, exercise, and selected other
diabetes medicines. It is not recommended as initial therapy in patients who have not achieved adequate
diabetes control on diet and exercise alone.
Liraglutide is an incretin mimetic. This means that it mimics the actions of incretin hormones in the
body. As an incretin mimetic, liraglutide increases insulin production in response to meals and decreases the
amount of glucose (sugar) that the liver produces. The medicine also slows the emptying of food from the
stomach and decreases the amount of food that people eat.
EXPERIMENTAL
Chemicals and Reagents:
HPLC grade Actonitrile, Methanol and Ortho Phosphoric Acid (OPA)were purchased from Merck
Specalities Pvt. Ltd.
Instrumentation and Analytical Conditions:
The analysis of drug was carried out on a PEAK HPLC system equipped with a reverse phase C18
column (250x4.6mm, 5µm in particle size), a LC-P7000 isocratic pump, a 20µl injection loop and a LC-UV7000
absorbance detector and running on PEAK Chromatographic Software version 1.06. Isocratic elution with
0.1% OPA: Acetonitrile: Methanol 30:40:30 (V/V) (PH-5.5) was used at a flow rate of 1.0ml/min. The mobile
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IJSIT (www.ijsit.com), Volume 1, Issue 1, September-October 2012
3. Alavala Siva Madhavi et al., IJSIT, 2012, 1(1), 17-26
phase was prepared freshly and degassed by sonicating for 5 min before use.
Stock and Working Standard Solutions:
10mg of Liraglutide working standard was accurately weighed and transferred into a 10ml
volumetric flask containing diluent. It was sonicated, dissolved completely and made volume up to the mark
with the same solvent. 1ml of the above stock solution was pipetted into a 10ml volumetric flask and diluted
up to the mark with diluent. The contents were mixed well and filtered through 0.45µm nylon filter paper and
60ppm was prepared finally. The calibration curve was plotted with the six concentrations of the 30 ppm-90
ppm working standard solutions. Calibration solutions were prepared daily and analyzed immediately after
preparation.
Assay of Liraglutide Tablets:
20 Liraglutide tablets were weighed and the average weight was calculated. The sample equivalent to
10mg of Liraglutide was accurately weighed and transferred in to a 10ml volumetric flask. The diluent was
added, sonicated for complete dissolution. The mixture wasmade volume up to the mark with diluents. They
were mixed well and filterd through 0.45um filter. Further, 1ml of the above stock solution pipetted into a
10ml volumetric flask and diluted up to mark with diluents and finally 60 ppm were prepared. They were
mixed well and filterd through 0.45um filter. An aliquot of this solution was injected into HPLC system. Peak
area of Liraglutide was measured for the determination.
Validation Procedure:
The objective of the method validation is to demonstrate that the method is suitable for its intended
purpose as it is stated in ICH guidelines. The method was validated for linearity, precision (repeatability and
intermediate precision), accuracy, specificity, stability and system suitability. Standard plots were
constructed with five concentrations in the range of 30 ppm to 90 ppm prepared in triplicates to test
linearity. The peak area of Liraglutide was plotted against the concentration to obtain the calibration graph.
The linearity was evaluated by linear regression analysis that was calculated by the least square regression
method. The precision of the assay was studied with respect to both repeatability and intermediate precision.
Repeatability was calculated from five replicate injections of freshly prepared Liraglutide test solution in the
same equipment at a concentration value of 100% (60 ppm) of the intended test concentration value on the
same day. The experiment was repeated by assaying freshly prepared solution at the same concentration
additionally on two consecutive days to determine intermediate precision. Peak area of the Liraglutide was
determined and precision was reported as %RSD.
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4. Alavala Siva Madhavi et al., IJSIT, 2012, 1(1), 17-26
Method accuracy was tested (% recovery and %RSD of individual measurements) by analyzing
sample of Liraglutide at three different levels in pure solutions using three preparations for each level. The
results were expressed as the percentage of Liraglutide recovered in the samples. Sample solution short term
stability was tested at ambient temperature (20±100C) for three days.
RESULT AND DISCUSSION
Optimization of the Chromatographic Conditions:
Proper selection of the stationary phase depends up on the nature of the sample, molecular weight
and solubility. Among C8 and C18, C18 column was selected. Non-polar compound is very attractive with
reverse phase columns. So the elution of the compound from the column was influenced by polar mobile
phase. Mixture of 0.1% OPA, Methanol and Acetonitrile was selected as mobile phase and the effect of
composition of mobile phase on the retention time of Liraglutide was thoroughly investigated. The
concentration of the 0.1% OPA, Methanol and Acetonitrile were optimized to give symmetric peak with short
run time (Fig.2).
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IJSIT (www.ijsit.com), Volume 1, Issue 1, September-October 2012
5. Alavala Siva Madhavi et al., IJSIT, 2012, 1(1), 17-26
Fig 2: Typical chromatogram of Liraglutide standard
Validation of Method
Linearity:
Five points graphs was constructed covering a concentration range 3-18ppm (Three independent
determinations were performed at each concentration). Linear relationships between the peak area signal of
Liraglutide the corresponding drug concentraton was observed. The standard deviation of the slope and
intercept were low. The statistical analysis of calibration is shown in Table 1.
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IJSIT (www.ijsit.com), Volume 1, Issue 1, September-October 2012
6. Alavala Siva Madhavi et al., IJSIT, 2012, 1(1), 17-26
S.NO Concentration in ppm Area
1 30 125697
2 40 167238
3 50 213028
4 60 250034
5 70 298632
6 80 342413
7 90 377098
Range 30 – 60 ppm Slope 4226
Intercept -653
Correlation coefficient 0.999
Table 1: Linearity of Liraglutide
400000
350000
300000
250000
200000
Area
150000
100000
50000
0
0 20 40 60 80 100
-50000
Concentration
Fig 3: Calibration curve of Liraglutide
Precision:
The validated method was applied for the assay of commercial tablets containing Liraglutide. Sample
was analyzed for five times after extracting the drug as mentioned in assay sample preparation of the
experimental section. The results presented good agreement with the labeled content. Low values of standard
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7. Alavala Siva Madhavi et al., IJSIT, 2012, 1(1), 17-26
deviation denoted very good repeatability of the measurement. Thus it was showing that the equipment used
for the study was correctly and hence the developed analytical method is highly repetitive. For the
intermediate precision a study carried out by the same analyst working on the same day on two consecutive
days indicated a RSD of 0.3135. This indicates good method precision.
% % Recovery Mean
Concentration Recovery
50% 99.67
100% 99.29 99.31 %
150% 98.97
Table 2: Recovery studies of Liraglutide
System Suitability:
The system suitability parameter like capacity factor, asymmetry factor, tailing factor and number of
theoretical plates were also calculated. It was observed that all the values are within the limits (Table.3). The
statistical evaluation of the proposed method was revealed its good linearity, reproducibility and its
validation for different parameters and let us to the conclusion that it could be used for the rapid and reliable
determination of Liraglutide in tablet formulation. The results are furnished in Table 3.
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IJSIT (www.ijsit.com), Volume 1, Issue 1, September-October 2012
8. Alavala Siva Madhavi et al., IJSIT, 2012, 1(1), 17-26
Parameters Values
λ max (nm) 245nm
Beer’s law limit (ppm) 30 – 90 ppm
Correlation coefficient 0.999
Retention time 6.11
Theoretical plates 11453
Tailing factor 1.26
Limit of detection 0.06ppm
Limit of quantification 0.2 ppm
Slope 4226
Intercept -653
Accuracy 99.31
R.S.D. 1.13
% Estimation of Liraglutide in 98.97%
formulation
Table 3: System stability parameters
Formulation Label claim (mg) % Amount found
Victoza 1.8 mg 98.97%
Table 4: Assay
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IJSIT (www.ijsit.com), Volume 1, Issue 1, September-October 2012
9. Alavala Siva Madhavi et al., IJSIT, 2012, 1(1), 17-26
Mobile phase Methanol:Acetonitrile:0.1% OPA
(35:60:5)
PH 5.1
UV detection 245nm
Analytical column C18
Flow rate 1.0ml/min
Temperature ambient
Injection volume 20µl
Runtime 10 min
Retention time 6.11 min
Table 5: Chromatographic condition
CONCLUSION
A validated RP-HPLC method has been developed for the determination of Liraglutide in tablet
dosage form. The proposed method is simple, rapid, accurate, precise and specific. Its chromatographic run
time of 10 min allows the analysis of a large number of samples in short period of time. Therefore, it is
suitable for the routine analysis of Liraglutide in pharmaceutical dosage form. The limit of detection for
Liraglutide was found to be 0.06 ppm and the limit of quantification was found to be 0.2 ppm. It proves the
sensitivity of method.
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10. Alavala Siva Madhavi et al., IJSIT, 2012, 1(1), 17-26
REFERENCES
1. http://www.novonordisk.com/science/about_rd/quarterly_rd_update.asp Oct 2008 Inc results of
LEAD 6 extension
2. http://money.cnn.com/news/newsfeeds/articles/marketwire/0580389.htm January 2009
3. Hirschler, Ben (January 13, 2010). "UPDATE 1-Novo starts tests on pill version of Victoza drug".
Reuters.
4. "Sector Snap: New Diabetes Drugs under FDA Review". Forbes. Retrieved March 27, 2009.
5. http://www.fda.gov/downloads/AdvisoryCommittees/CommitteesMeetingMaterials/Drugs/Endocr
inologicandMetabolicDrugsAdvisoryCommittee/UCM148659.pdf
6. Victoza Package Insert Date of Issue: January 2010 Version: 1
7. N Engl J Med, 362:774
8. http://www.drugs.com/fda/victoza-liraglutide-rdna-origin-rems-risk-thyroid-c-cell-tumors-acute-
pancreatitis-12979.html
9. http://diabetes.webmd.com/news/20080924/new-diabetes-drug-liraglutide-works Sept 2008
10. http://care.diabetesjournals.org/cgi/content/abstract/32/1/84 "Efficacy and Safety Comparison of
Liraglutide, Glimepiride, and Placebo, All in Combination with Metformin, in Type 2 Diabetes"
Diabetes Care. Oct 2008
11. "Novo Nordisk Limited, Eli Lilly and Company Limited, Grünenthal Ltd and Napp Pharmaceuticals
Limited named in advertisements". Prescription Medicines Code of Practice Authority (PMCPA).
Retrieved 2011-02-07.
12. http://www.mmm-online.com/novo-reps-to-spotlight-weight-loss-for-victoza-
launch/article/163167/ | Medical Media & Marketing; Novo reps to spotlight weight loss for Victoza
launch; 2/4/2010; Ben Comer.
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IJSIT (www.ijsit.com), Volume 1, Issue 1, September-October 2012