A reverse phase HPLC method was developed and validated for the quantification of lamivudine in bulk and tablet formulations. The method used an ODS column with a mobile phase of acetate buffer and acetonitrile (50:50) at a flow rate of 1.5 mL/min. Lamivudine had a retention time of 1.85 minutes when detected at 272 nm. The method was linear over a concentration range of 10-50 μg/mL with a correlation coefficient of 0.999. Accuracy and precision studies demonstrated recoveries between 98-102% and %RSD below 2%, respectively. The method was found to be robust, specific, and suitable for the routine analysis of lamivudine in