Urine is the body's liquid waste composed of water, salt urea and uric acid. Urine can be evaluated by its physical appearance (color, cloudiness, odor, clarity), also referred to as a macroscopic analysis. It can be also analyzed based on its chemical and molecular properties, including microscopic assessment.
Urine is the body's liquid waste composed of water, salt urea and uric acid. Urine can be evaluated by its physical appearance (color, cloudiness, odor, clarity), also referred to as a macroscopic analysis. It can be also analyzed based on its chemical and molecular properties, including microscopic assessment.
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Automation in clinical chemistry: Principle, Instrumentation and benefits. 7. ... Collection and transportation of urine sample.
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WHAT IS URINE ANALYSIS?
Urine analysis, also called Urinalysis – one of the oldest laboratory procedures in the practice of medicine.
Also knows as Urine- R&M (routine & microscopy)
Is an array of tests performed on urine
WHY URINALYSIS?
General evaluation of health
Diagnosis of disease or disorders of the kidneys or urinary tract
Diagnosis of other systemic disease that affect kidney function
Monitoring of patients with diabetes
Screening for drug abuse (eg. Sulfonamide or aminoglycosides)
COLLECTION OF URINE SPECIMENS
Improper collection---- may invalidate the results
Containers for collection of urine should be wide mouthed, clean and dry.
Analyzed within 2 hours of collection else requires refrigeration.
URINE CULTURE
Culture within 1 hour after collection or stored in a refrigerator at 4oC for no more than 18 hours.
Culture is performed when Polynephritis or Cystitis is suspected.
UTI is most frequent caused by E.Coli.
Other common agents are Enterobacter, Proteus, and Enterococcus faecalis.
URINALYSIS; WHAT TO LOOK FOR?
• Urinalysis consists of the following measurements:
Macroscopic or physical examination
Chemical examination
Microscopic examination of the sediment
Urine culture
PHYSICAL EXAMINATION OF URINE
Examination of physical characteristics:
Volume
Color
Odor
pH
Specific gravity
The refractometer or a reagent strip is used to measure specific gravity
PHYSICAL EXAMINATION
Normal- 1-2.5 L/day
Oliguria- Urine Output < 400ml/day
Dehydration
Shock
Acute glomerulonephritis
Renal Failure
Polyuria- Urine Output > 2.5 L/day
Increased water ingestion
Diabetes mellitus and insipidus.
Anuria- Urine output < 100ml/day
Seen in renal shut down Volume
PHYSICAL EXAMINATION
Normal
pale yellow in color due to pigments urochrome (different colour pigments in urine), urobilin (When urobilinogen- degraded product of bilirubin, is exposed to air, it is oxidized to urobilin, giving urine its yellow color) and uroerythrin (red pigment in urine).
Cloudiness
may be caused by excessive cellular material or protein, crystallization or precipitation of non pathological salts upon standing at room temperature or in the refrigerator.
Color
Colour of urine depending upon it’s constituents.
PHYSICAL EXAMINATION
Abnormal Colors:
Colorless – diabetes, diuretics.
Deep Yellow – concentrated urine, excess bile pigments, jaundice Color
Blue-Green – Methylene Blue, Pseudomonas (Bactrium), Riboflavin (Vitamin B2, in FAD give Yellow Orange Color)
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2. INDICATIONS FOR URINALYSIS:
1. Suspected renal diseases like glomerulonephritis, nephrotic
syndrome, pyelonephritis, and renal failure
2. Detection of urinary tract infection
3. Detection and management of metabolic disorders like diabetes
mellitus
4. Differential diagnosis of jaundice
5. Detection and management of plasma cell dyscrasias
6. Diagnosis of pregnancy
3. TIME OF COLLECTION OF URINE:
• First morning, midstream: Preferred for routine urine
examination.
• Random, midstream: Routine urine examination.
• First morning, midstream, clean catch: Bacteriological
examination.
• Postprandial: Estimation of glucose, urobilinogen
• 24-hour: Quantitative estimation of proteins or hormones.
• Catheterised: Bacteriological examination in infants,
bedridden patients, and in obstruction of urinary tract.
• Plastic bag tied around genitals : infants, incontinent adults .
4. Collection for routine urinalysis:
• For routine examination of urine, a wide-mouthed glass bottle of 20-
30 ml capacity, which is dry, chemically clean, leakproof, and with a
tight fitting stopper is used. About 15 ml of midstream sample is
cleanly collected.
Collection for bacterial culture:
• Use sterile container Collect midstream, clean catch sample Must be
plated within 2 hours of collection If refrigerated, must be plated
within 24 hours of collection
5. VOLUME:
• Volume of only the 24-hr specimen of urine needs to be measured
and reported. The average 24-hr urinary output in adults is 600-2000
ml. The volume varies according to fluid intake, diet, and climate.
Abnormalities of urinary volume are as follows:
• Polyuria means urinary volume > 2000 ml/24 hours.
This is seen in diabetes mellitus (osmotic diuresis ,diabetes
insipidus (failure of secretion of antidiuretic hormone), chronic
renal failure (loss of concentrating ability of kidneys) or diuretic
therapy.
6. • Oliguria means urinary volume < 400 ml/24 hours
Causes include febrile states, acute glomerulonephritis
(decreased glomerular filtration), congestive cardiac failure or
dehydration (decreased renal blood flow).
• Anuria means urinary output < 100 ml/24 hours or complete
cessation of urine output. It occurs in acute
tubular necrosis (e.g. in shock, hemolytic transfusion reaction),
acute glomerulonephritis, and complete urinary tract
obstruction.
7. ODOUR:
• Freshly voided urine has a typical aromatic odour due to volatile
organic acids. After standing, urine develops ammoniacal odour
(formation of ammonia occurs when urea is decomposed by
bacteria).
Some abnormal odours with associated conditions are:
• Fruity: Ketoacidosis, starvation
• Mousy or musty: Phenylketonuria
• Fishy: Urinary tract infection with Proteus, tyrosinaemia.
• Ammoniacal: Urinary tract infection with Escherichia coli, old
standing urine.
• Foul: Urinary tract infection
• Sulfurous: Cystinuria.
8. COLOUR :
Normal urine color in a fresh state is pale yellow or
amber and is due to the presence of various pigments
collectively called urochrome. Depending on the state
of hydration urine may normally be colorless(over
hydration) or dark yellow (dehydration).
9. Some of the abnormal colors with associated
conditions are:
• Colourless Dilute urine: diabetes mellitus, diabetes insipidus, overhydration
• Red: Haematuria, Haemoglobinuria, Porphyria, Myoglobinuria
• Dark brown or black: Alkaptonuria, Melanoma
• Brown: Haemoglobinuria
• Yellow: Concentrated urine
• Yellow-green: Biliverdin
• Deep yellow with yellow foam: Bilirubin
• Orange or orange brown: Urobilinogen, Porphobilinogen
• Milky-white: Chyluria
• Red or orange fluorescence with UV light: porphyria
Note: Many drugs cause changes in urine colour; drug history should be obtained
10. SPECIFIC GRAVITY:
• This is also called as relative mass density. It depends on amount of
solutes in solution. It is basically a comparison of density of urine
against the density of distilled water at a particular temperature.
Specific gravity of distilled water is 1.000.
• Normal SG of urine is 1.003 to 1.030 .
• SG of normal urine is mainly related to urea and sodium. SG increases
as solute concentration increases and decreases when temperature
rises (since volume expands with rise in temperature).
• SG of urine is a measure of concentrating ability of kidneys and is
determined to get information about this tubular function.
11. • Causes of increase in SG of urine :
diabetes mellitus (glycosuria), nephrotic syndrome
(proteinuria), fever, and dehydration.
• Causes of decrease in SG of urine :
diabetes insipidus (SG consistently between 1.002-1.003),
chronic renal failure (low and fixed SG at 1.010 due to loss of
concentrating ability of tubules) and compulsive water drinking.
12. URINOMETER METHOD :
The method is as follows:
1. Fill a measuring cylinder with 50 ml of urine,3/4 th
2. Lower urinometer gently into the urine and let it float freely.
3. Let urinometer settle; it should not touch the sides or bottom of the
cylinder.
4. Take the reading of SG on the scale(lowest point of meniscus) at the
surface of the urine.
13. APPEARANCE:
Normal, freshly voided urine is clear in appearance.
Foamy urine occurs in the presence of excess proteins or bilirubin.
1. Amorphous phosphates: White and cloudy
2. Amorphous urates: Pink and cloudy
3. Pus cells: Varying grades of turbidity
4. Bacteria: Uniformly cloudy; do not settle at the bottom .
14. REACTION/pH:
• It reflects the ability of kidney to maintain H+ ion concentration in
extracellular fluid and plasma.
• It can be measured by electronic pH meter or by pH indicator paper.
• Freshly voided normal urine is slightly acidic and its pH ranges from
4.6 to 7.0 (average 6.0)
Acidic: High protein intake ,acidic fruits intake, metabolic acidosis, UTI
by E coli
Alkali : Citrus fruits, vegetables, metabolic alkalosis, UTI by proteus and
pseudomonas