This document discusses micropropagation of banana (G-9) for commercial cultivation. It begins by outlining the nutritional value and various products of bananas. Next, it describes the limitations of traditional propagation methods using root suckers and advantages of micropropagation using tissue culture, which allows for large-scale, disease-free propagation. The document then details the specific micropropagation method used, including establishment of primary culture, shoot multiplication, rooting, and hardening/acclimatization. Finally, it proposes cost-effective solutions for some materials used, such as LED lights, table sugar, agar-agar type-II, RO water, and cocopeat. The overall aim is sustainable development of a banana industry through efficient

1. Cost effective
micropropagation of Banana
Industrial Internship
Case study
GSFC University
Vadodara, Gujarat, India

2. Tree of Heaven
2

3. 3
Introduction

4. Vitamins
Vitamin E (α-Tocopherol), Vitamin
C, Vitamin B6, thiamin riboflavin,
niacin
Rich source of energy
Rich source of minerals and carbs
Increase happiness
Increase serotonin
Amino acids
About 17 essential amino acids
01
02
03
04
Banana:
Nutritional
value

5. 5

6. Various Banana Products
6

7. Source: https://qz.com/164029/tropical-race-4-global-banana-industry-is-killing-the-worlds-favorite-fruit/ 7

8. 8

9. 9

10. Demand
10

11. 11

12. 12

13. 13

14. 14

15. 15

16. Market
Demand
₹
USD $ 150 mn
Others
USD $ 310 mn
Food industry
USD $ 277.2 mn
Organic banana
US$ 68.22 mn
Flour
USD $ 117.97 mn
Paper
Banana
demand:
Worldwide

17. 17

18. Top ten banana producing countries in the world, in terms of
volume of production, CY 2002 (FAO).
18

19. 19
Banana Cultivation In India

20. 20
Agro-climatic conditions

21. 21
SOIL

22. 22
Why Grand Nain (G9)?

23. Selection of
Elite Banana
sp.
Product
Cultivation Marketing
Identification and
selection of most
suitable species as
per requirement
Value added Products
like Jam, Biscuits,
confectionary items,
papers etc
Application of
micropropagation
techniques for
quality planting
material
Customer
identification,
distribution,
advertisements
Sustainable development of a Banana Industry

24. Image Source: Shree Bharat Rajput,Prem Sarita Agri Biotech- Rajpipla
24

25. Root sucker, however plantlet produced via vegetative propagation
carries diseased from mother plant 01
Limited number of suckers are produced per plant (5-7 per
year)
02
Required more space and bulky to transport
03
Risck of variety mix-up
04
Natural
method
of
Cultivation

26. 26
What Is Plant Tissue Culture?

27. Tissue culture raised plants are more suitable for long
distance transport
Starting with 50 suckers; able to produce 70,000
annual clones. Start with 200 tissue culture vials;
produce 2 million annual clones.
Uses 1/10 the space of traditional cloning. Two million
annual clones could be produced in less than 3000
square feet.
1000 mother cultivars could be stored inside a
refrigerator with no care or maintenance for months,
sometimes over a year
Propagation is significantly more efficient
27

28. Method: Micropropagation of Banana (G-9)
Method
• Selection of elite stock (Mother plant): High
yielding and disease free
• Collection of suckers from mother plant
• Surface sterilization of sucker with the help of
antibiotics or mercuric chloride.
• Establishment of primary culture from sucker
on MS medium with suitable plant growth
regulators and appropriate laboratory
conditions.
• Shoot multiplication and maintenance
• In vitro rooting of shoots.
• Primary hardening (acclimatization) in mist
chamber
• Secondary hardening (acclimatization) in net
house
• Genetic fidelity and pathogen indexing.
• Marketing and distribution to farmers 28

29. A
C
B C
D E F
Micropropagation of Banana (G-9)
Figure 1 A-C: Establishment of
primary culture using root
suckers.
Figure 1 D-E: Multiplication stage
Figure 1 F: Multiplication stage
Image Source: Shree Bharat Rajput,Prem Sarita Agri Biotech- Rajpipla
29

30. Figure 2 A: Ex agar plants.
Figure 2 B: Primary hardening in
low tunnel with mist chamber
Figure 2 C-F: Secondary
hardening in net house.
Image Source: Shree Bharat Rajput,Prem Sarita Agri Biotech- Rajpipla
A B C
D E F
30

31. 31
COST EFFECTIVE SOLUTION
Item Options
available
Present work Cost
Light source While
fluorescent
tubes/ Halogen
bulb
LEDs used (Low energy
consumption )
LEDs use 25%–30% of the energy and last 8 to
25 times longer than halogen (Energy.gov
2021)
Carbohydrate
source
Sucrose Table sugar cubes from
local market
Labogen AR Grade sucrose price is 15.16 US$
per kg while, Table sugar cube cost is 1 US$
per kg
Gelling agent Agar or Gelrite
or Phytagel
Agar-agar type-II low
cost
50% less price
Water Distilled water Reverse osmosis (RO)
water
Distilled water systems are more expensive to
operate and take longer to filter water than RO
system. Applequist (2018)
Grade of
Chemicals
Analytical Laboratory grade Laboratory grade chemicals are having low
cost
Acclimatization
matrix
Soilrite/Rockwoo
l/Perlite
cocopeat/Moss
Only cocopeat Cocopeat is less expensive as compare with
perlite

32. 32
Conclusion

33. 33
References