3. 3
CHOLERA OUTBREAK - Karaikal
People have been advised to follow hygiene
practices like consuming boiled water for drinking,
washing hands regularly, taking safe drinking water
in public places and hotels, using a public toilet and
avoiding open-air defecation, reporting any local
government Primary Health Centres
(PHC)/Community Health Centres (CHC) in case if a
person comes across any symptoms.
July 2022
5. Water Purification
5
Small Scale Methods Large Scale Methods
2. Well Disinfection
3. Storage
1. Filtration ( Rapid and slow
sand filters)
1. Household purification of water
(Boiling , Chemical disinfection)
2. Disinfection of water
6. Disinfection Vs Sterilisation
Methods of disinfection
Definitions and Sources of Chlorine
Chlorination of water and its uses
Instruments and tests for chlorination
Swimming pool and OHT sanitation
Related Health programmes
8. • 1. Boiling - Best method – kills organisms and spores
• 2. Chlorination ( 2nd best) ----- Cl2 gas , Bleaching powder , Chlorine dioxide, Chlorine
tablets, Chloramines ( slow acting) – used in disinfection of water and surgical instruments
• 3. Ozonation
• 4. UV rays treatment
• 5. Membrane Process – RO filters
Methods of Disinfection of Water:
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9. 2nd best because ( high doses needed on certain pathogens)
Not effective in small doses on
• Poliomyelitis
• Hepatitis A , Cryptosporidium , Helminth cysts and ova
• Bacterial spores and cyclops
9
CHLORINATION:
Disinfection property is mainly
due to HOCL ( 90%) > Ocl (10%)
10. PRINCIPLES & PROPERTIES :
10
DRINKING WATER CHLORINE ( CHLORINATED WATER)
Colorless Bactericidal , moderate virucidal and not sporicidal
Odorless with mild taste Best method in rural areas
Not turbid Very high ( <2ppm) – GI issues, bladder cancer , throat
irritation)
Free from pathogens, Floating
matter and suspended particles
Free/ Residual action – remains effective for next 2days
(safety during storage / distribution) ( unique feature)
PH -8 ( Slightly alkaline) Acts best at PH of 7 – most WBD diseases also the same
PH >8.5 – 90% of HOCL gets ionized to hydrogen ions
and hypochlorite ions
TDS <500 ppm 33% chlorine available in 100 g bleaching powder
11. • 1. Chlorine demand: Amount of Chlorine needed to destroy bacteria and oxidize all
organic matter and ammoniacal substances in water
• 2. Breakpoint chlorination : The point at which the chlorine demand is met ---on further
adding Cl beyond– it settles down as Free residual chlorine
• 3. Super chlorination : Addition of large doses of chlorine to heavily contaminated water
and removal of excess of chlorine after disinfection
• 4. Stabilised bleach : Bleaching powder mixed with excess of lime – retains strength
( bleaching powder exposed to sunlight /moistures --- loses it Cl- content)
SOME BASIC DEFINITIONS………..
11
13. 13
FREE RESIDUAL CHLORINE LEVELS
Ppm ( mg/dl)
RECOMMENDED AREAS FOR CONTACT PERIOD
( 1 hr )
>0.5 ppm In Drinking water at home
>0.7 ppm Post disaster and water bodies ( outbreaks)
>1 ppm Swimming pools
>2 ppm To kill Cyclops
14. HORROCK’S APPARATUS
14
Used for estimating the dose of bleaching powder needed to disinfect 455 Liters of
water --- Chlorine demand ( Mostly in wells)
15. • Contents of ‘Horrock’s Apparatus’
• 6 white cups, each of 200 ml capacity
• One black cup with a circular mark on the inside
• 2 metal spoons (each holds 2g of bleaching powder when filled up to the brim)
• 7 glass stirring rods
• One special pipette
• Two droppers
• Starch - iodide indicator solution
• Instruction folder
• Steps in Estimating the Required Dosage of Bleaching Powder for disinfecting water
1. One level spoonful (2g) of bleaching powder is placed in the black cup and made into a thin paste with a little water
The solution thus prepared is the ‘Chlorine Stock Solution’
2. The six white cups are filled with the water to be tested
3. Using the pipette provided with the apparatus, add one drop of the stock solution to the first cup, two drops to the
second cup, three drops to the third cup, and so on till adding six drops to the sixth cup.
• 4. Stir the water in the cups; use a separate rod for each cup
5. Wait for half an hour
6. Add three drops of starch-iodide indicator to each of the white cups
7. Development of blue color indicates the presence of free residual chlorine.
15
16. 16
INTERPRETATION :
If first cup develops blue colour: 1 spoonful (i.e. 2gm) of bleaching powder would suffice for
455 L of water
If the second cup develops blue colour: 2 spoonfuls (4 gm) of bleaching powder for 455 L of
water etc…..
If none of the cups develop a blue colour, this means that the chlorine demand of the water is
very high and it is better to use some method of clearing the water prior to chlorinating it
• If nth cup shows blue colour = n x 2g of bleaching powder is required for disinfection
Required Bleaching powder (gm) = Volume of water in litres x n x 2
455
17. 17
How many grams of bleaching powder is required to disinfect 3000 Litres of
water where 4th cup shows blue color change in Horrocks apparatus ?
53 g
18. 1. Orthotoluidine Test :
To check whether the water is chlorinated or not
Determines both Free and combined chlorine in water together
0.1 ml of reagent added to water with chlorine
Reading taken with 10 sec ( Free Cl-) and after 15-20 min ( Free and combined Cl)
Yellow color – matched with chloroscope
2. Orthotoluidine Arsenite Test :
Better test – since Free and Combined chlorine estimation separately
Unaffected by impurities ( Nitrites , iron , manganese)
TESTS FOR CHLORINATION :
18
20. • 400 ml of water mixed with 0.25g chlorine dioxide powder
• Dissolve and stir for few min
• Take 10ml of sample solution in test tube and add 2drops of OT reagent
• Turns yellow color measured against chloroscope - presence of level of chlorine
in water
Testing Chlorine content in water at Home level
20
21. • At field level – HI - once in 15days and daily in epidemics
• Bleaching powder kept tightly packed in dark room
• Sample of water taken and tested ( OT test along with chloroscope)
• Check the level of chlorination in water in tank
• Water added with Bleaching powder mixed like paste and diluted with water ( Chlorine solution)
• Chlorine water separated carefully by filtration
• Climb up and do chlorination for 1hr ---after that recheck with chloroscope again
• 1000 L water requires 4g bleaching powder ( even at syntex tank in home)
OVERHEAD TANK CHLORINATION
21
22. Swimming Pool Sanitation :
22
HOCl –penetrate cell walls of bacteria/viruses- kills it
Must be equipped with Rapid sand filter ( filtration <6hrs)
Area > 24 sqft / swimmer
IEC posters for regulations posted in appropriate places
>15% water to be changed every day
Free from communicable diseases – allow to swim
Before swimming –empty bladder / use toilet outside pool
Proper chlorination of the pool (> 1ppm free Cl-)
Maintain pH of 7.4-7.8
23. All these techniques have No residual action unlike chlorination
1. OZONATION
• Powerful oxidant ----- strong virucidal – O3 gas ( 0.2-1.5 mg/dl)
• Used for chemical contamination of pesticides
2. UV rays treatment – 254 nm UV light damages RNA and DNA of organisms
3.Membrane process : Done in RO Filters using reverse osmosis , micro/ nano filtration techniques
OTHER METHODS OF DISINFECTION OF WATER :
24. 1. > 1.6 km access to water supply in rural plain areas and > 100m in hilly areas
2. >15m depth of water source ( bore)
3. Excess Salanity ( physical/ chemical contamination)
4. Water exposed to cholera / Guinea worm
Problem Village - Criteria
24
27. 1. All the following statements are true about breakpoint chlorination EXCEPT?
A. Free chlorine is released into water after breakpoint
B. Chlorine demand is amount of chlorine needed to kill bacteria , oxidise organic
matter and neutralize ammonia
C. 1 ppm free chlorine should be present in water after breakpoint has reached
D. Most commonly used form of chlorine is bleaching powder
2. Method of choice for purification of highly polluted water on large scale ?
A. Boiling
B. Chlorination
C. Superchlorination followed by Dechlorination
D. UV light treatment
MCQs
27
28. 3. Minimum recommended dose of free residual chlorine in water for routine chlorination ?
A. 0.5 ppm for a contact period of 1hr
B. 0.5 ppm for a contact period of 30min
C. 1 ppm for a contact period of 1hr
D. 2 ppm for a contact period of 30min
4. The colour produced in OT test is due to action of
A. Dissolved solids
B. Free chlorine
C. Combined chlorine
D. Free and combined chlorine
MCQ
28
29. 5. Access to water supply in Problem village criteria for distance in plain areas ?
A. >1.2kms
B. >1.5kms
C. >1.6kms
D. >1.7kms
6. Chlorination in small doses is not an effective disinfectant in all EXCEPT?
A. Polio virus
B. Bacterial cysts
C. Salmonellosis
D. Cyclops
MCQ
29
30. 7. Chlorine acts best at pH of ?
A. 6
B. 8
C. 7
D. 9
8. Minimum level of Chlorination in swimming pools ?
A. 1ppm
B. 1.5ppm
C. 2ppm
D. 0.5ppm
MCQ
30
31. Days to Remember..
• World Water Day- 22nd March
( 2023 THEME ---- “Accelerating Change”)
• International Decade for “Water for Life”
(2005 – 2015)
• Water Conservation Day - 19th November
32. Water Saving Tips in Home
Check at home for leaks, hidden
water leaks
Fix any dripping tap
Turn off tap while cleaning your teeth, shaving
or washing your face
Only use the washing machine when you can
put on a full load.
Use a sponge and bucket of water to
clean your car
Water and health integration --------Fluoride content less or more ,,,,,,Nitrates , Chlorine , and diseases spread and hardness
Large scale ---cities /towns
STERILISATION ----COMPLETE killing of organism including spores
Disinfection ------ reduction in harmful disease causing pathogens ( not its spores)
Cl plus H20 ----HOCL ?( PH 5-7)
100 g bleaching powder mixed with water gives 33g Cl-
WBD ---Water borne disease – leptospirosis , hepatitis , cholera, diarrohea , polio
CHLORINE DEMAND ----how much chlorne needed for water disinfection
Bleaching powder so kept in dark room
Phases of chlorination
On increasing chlorine ---chloramines formation ---after chlorine added more--- destruvtion ----reaches breakpt after further adding chlorine ---after tht FRC
Ppm = mg/dl
DONE BY HI ( wears gloves along with tank operator ) --- chlorination IN tanks – prevents 90% of WBD
Real time video of HI in youtube – TN
Better to drink boil water though chlorination done
Health hazards----URI , fungal , ENT Infections and eye infections
The states of Goa, Telangana, Haryana & UTs of Andaman & Nicobar Islands and Puducherry, D&NH and D&D have ensured 100% household tap connection in rural areas