Plant tissue culture part 2
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This document discusses the key steps in plant tissue culture, including formulation of the culture medium, collection and surface sterilization of explant materials, inoculation of explants into the culture medium, incubation of explants to allow for cell division and callus formation, and hardening of plantlets before transferring them to outdoor conditions. The culture medium must contain carbon, macronutrients, micronutrients, and plant hormones. Explants are collected from plant parts like roots, stems, leaves, and embryos, then sterilized using detergents, alcohol, and mercuric chloride to remove microbes. Explants are inoculated aseptically into the culture medium and incubated to induce cell division and callus
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Plant tissue culture
Hello ever one i hope its useful for preparation of notes regarding plant tissue culture for Pharmacognosy .. B.pharm II yr IV sem.. plz give comments it may useful for me and i can rectify the things.
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The document discusses preparation of plant tissue culture media, types of media, and sterilization techniques. It covers:
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Plant tissue culture involves growing plant cells, tissues or organs under sterile conditions on a nutrient medium. It has a history dating back to the 1930s and was pioneered by scientists like Haberland and White. Techniques include using explants from plants, sterilizing them, and culturing them on nutrient media containing salts, sugars and growth regulators. This allows for dedifferentiation of cells and regeneration of whole plants. Micropropagation specifically refers to rapidly multiplying shoots in culture. It has five stages - selection of stock plants, initiation of culture, shoot multiplication, rooting, and hardening. It has advantages like producing many pathogen-free plants quickly from small explants.
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Plant tissue culture involves growing plant cells, tissues or organs in an artificial nutrient medium under sterile conditions. Some key points:
1. It allows for the rapid mass propagation of plants through micropropagation and the production of genetically uniform plants.
2. It facilitates the production of disease-free plants through culture of meristems and shoot tips.
3. It enables genetic modification of plants through techniques like protoplast fusion, anther culture and recombinant DNA technology.
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Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on nutrient media. Some key points:
- Callus culture involves growing an unorganized cell mass (callus) from explants on auxin-cytokinin media, which can then be used for micropropagation.
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This document discusses plant tissue culture, including:
- Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on nutrient media to produce clones.
- It requires specialized lab facilities and sterile techniques to prevent contamination. Media must contain macros/micronutrients, carbohydrates, growth regulators and solidifying agents.
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Preparation of plant tissue culture media,types and Sterilization
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Plant tissue culture involves growing plant cells, tissues or organs under sterile conditions on a nutrient medium. It has a history dating back to the 1930s and was pioneered by scientists like Haberland and White. Techniques include using explants from plants, sterilizing them, and culturing them on nutrient media containing salts, sugars and growth regulators. This allows for dedifferentiation of cells and regeneration of whole plants. Micropropagation specifically refers to rapidly multiplying shoots in culture. It has five stages - selection of stock plants, initiation of culture, shoot multiplication, rooting, and hardening. It has advantages like producing many pathogen-free plants quickly from small explants.
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The ability of an explant to regenerate into a whole plant under in vitro asceptic conditions by providing a proper artificial nutrient medium is called as Plant Tissue Culture.
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This document discusses plant tissue culture techniques. It defines plant tissue culture as the maintenance or growth of plant cells, tissues, or organs under sterile conditions on a nutrient medium. The key steps are explained as preparation of a sterile medium, selection and sterilization of an explant, inoculation, incubation, and regeneration. Different types of culture are described, including callus culture and the use of tissue culture in micropropagation, genetic modification, and production of secondary metabolites. Growth regulators like auxins, cytokinins, and gibberellins are also discussed.
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This document discusses different types of plant cell and tissue cultures. It defines tissue culture as growing cells artificially in the laboratory and plant tissue culture as a technique to produce genetically identical plantlets. The key requirements for tissue culture include aseptic conditions, aeration, appropriate nutrient medium and explants. Types of tissue culture include callus, suspension and organ cultures. Cell culture is defined as growing cells under controlled conditions using artificial media. Steps in cell culture include sample collection, isolating target cells, choosing media, preventing contamination and cell harvesting. Protoplast culture refers to developing whole plants from cells without cell walls, with major steps including sterilization, peeling leaves, enzyme treatment and isolating protoplasts. Applications
Plant Tissue Culture
Plant tissue culture is a technique of growing plant cells, tissues, organs, seeds, or other plant parts in a sterile
environment on a nutrient medium.
Tissue culture had its origins at the beginning of the 20th century with the work of Gottlieb Haberlandt
(plants).
WHY PLANT TISSUE CULTURES ARE DONE ??
The production of clones of plants that produce particularly good flowers, fruits, or have other desirable traits.
To quickly produce mature plants.
The production of multiples of plants in the absence of seeds or necessary pollinators to produce seeds.
The regeneration of whole plants from plant cells that have been genetically modified.
The production of plants in sterile containers reduces disease transmission
Allows production of plants from seeds that otherwise have very low chances of germinating and growing, i.e.: orchids and Nepenthes.
To clean particular plants of viral and other infections and to quickly multiply these plants as 'cleaned stock' for horticulture and agriculture.
***For PTC, the laboratory must have the following facilities:
Washing facility for glassware and ovens for drying glassware.
Medium preparation room with autoclave, electronic balance and pH meter.
Transfer area sterile room with laminar air-flow bench and a positive pressure ventilation unit called High Efficiency Particulate Air (HEPA) filter to maintain aseptic condition.
Culture facility: Growing the explant inoculated into culture tubes at 22-28° C with illumination of light 2400 lux, with a photoperiod of 8-16 hours and a relative humidity of about 60%.
*****Based on the explants some other plant tissue culture types are:
1. Organ culture
2. Meristem culture
3. Protoplast culture
4. Cell culture.
Tissue culture
Plant tissue culture is a technique where plant cells, tissues, or organs are grown in an artificial nutrient medium under sterile conditions. Haberlandt in 1902 first attempted to culture plant tissues in vitro and is considered the father of plant tissue culture. Important milestones in the history of plant tissue culture include the development of MS medium by Murashige and Skoog in 1962 and the first transgenic plant created in 1983. Common types of plant tissue culture include callus culture, suspension culture, organ culture, and meristem culture. Tissue culture has many applications in crop improvement including micropropagation, breeding, and production of secondary metabolites.
Plant tissue culture
Plant tissue culture is a collection of techniques used to grow plant cells, tissues or organs under sterile conditions. It allows for the mass production of clones of plants with desirable traits. The key aspects of plant tissue culture are maintaining sterile conditions on a nutrient medium, and providing proper aeration. Common types of plant tissue culture include callus culture, single cell culture, root tip culture, shoot tip culture, and anther culture. Plant tissue culture has many applications for plant conservation, breeding, and production of secondary metabolites.
MODULE 03.pdf
The document discusses Module 3 which covers plant tissue culture and edible vaccines. It aims to teach students about plant tissue culture techniques including the basic requirements and general procedure. It also aims to teach students about the concept of edible vaccines including their advantages and disadvantages. The key learning outcomes are for students to understand plant tissue culture methodology, how to maintain a tissue culture lab, applications of plant tissue culture, and the concept, methods and applications of edible vaccines.
Plant tissue culture
Plant tissue culture involves growing plant cells, tissues, or organs in sterile conditions on a nutrient culture medium. It allows for rapid multiplication of plant materials and production of genetically identical clones. Various types of explants can be used, including shoot tips, roots, leaves, and embryos. The explants are first sterilized and induced to form callus tissue on auxin-supplemented medium. Shoots and roots can be regenerated from callus using different hormone combinations. Somatic embryos can also be produced through tissue culture.
Tissue culture and biotechnological aspects in plantation crops by lokesh
1. Tissue culture techniques allow for the propagation of plant cells and tissues on nutrient media and can be used for micropropagation and biotechnology applications in plantation crops like coconut and rubber.
2. Coconut micropropagation faces challenges like heterogeneous explant response, undefined culture conditions due to activated charcoal, and slow growth. Developing charcoal-free media and selecting the most responsive explants can help overcome these limitations.
3. Rubber tissue culture uses explants like shoot tips, anthers, and integuments and involves sterilization, culture on hormone-supplemented media, and transfer to soil. Somatic embryogenesis from anthers and inflorescences also allows mass propagation of rubber clones
PLANT TISSUE CULTURE.pptx
It gives the general knowledge about plant tissue culture. As this topic is an important aspects of plant biotechnology, it will remind a brief idea about why it is necessary.
Biotechnological production of natural products by Dr. Refaat Hamed
Plant cell and organ cultures can be used to produce valuable secondary metabolites. Three main approaches were discussed: 1) using plant cell and organ cultures, 2) using microbial cell factories, and 3) using molecular biopharming. Plant cell and organ cultures involve growing plant cells, tissues, or organs in vitro on nutrient media. This allows mass production of metabolites and can help address issues with traditional extraction. Microbial cell factories and molecular biopharming use genetically engineered microbes or organisms to produce metabolites. Taxol is an important anticancer drug produced through plant cell cultures due to supply issues with traditional extraction from yew trees.
Introduction about micropropagation
This document outlines the steps involved in micropropagation of plants through tissue culture:
1. Selection and sterilization of explant tissue
2. Establishment of explant in culture media
3. Multiplication of explants through cell division and formation of callus or shoots
4. Root formation through manipulation of growth regulators in culture media
5. Hardening and acclimatization of plantlets before transferring to soil.
Plant tissue culture
This document discusses plant tissue culture, including its definition, history, advantages, disadvantages, and procedures. Some key points:
- Plant tissue culture involves growing plant cells, tissues, or organs in a sterile nutrient medium under controlled conditions. It allows for the production of metabolites and regeneration of whole plants.
- The history of plant tissue culture dates back to the 19th century with early work on cell theory. Significant developments occurred throughout the 20th century, including the discovery of plant growth hormones and establishment of callus culture.
- Advantages include availability of raw materials, disease-free propagation, biosynthetic pathway studies, while disadvantages include requiring expertise, instability, and slow growth.
- Proced
Plant Tissue Culture - Medium preparation, Techniques, Types and Benefits.
Brief discussion about plant tissue culture, medium components and medium preparation, sterilization, techniques and types of plant tissue culture. Simplified discussion but well elaborated.
plant tissue culture- all notes
Essay on Plant Tissue Culture Contents:
the Definition of Plant Tissue Culture.
the History of Plant Tissue Culture.
the Basic Requirements of Plant Tissue Culture.
the General Techniques of Plant Tissue Culture.
the Basic Aspects of Plant Tissue Culture.
the Cellular Totipotency.
the Differentiation.
the Methods in Plant Tissue Culture.
the Applications of Plant Tissue Culture.
the Morphogenesis.
the Subculture or Secondary Cell Culture.
the Soma-Clonal Variation.
the Somatic Hybrids and Cybrids.
the Micro-Propagation.
the Artificial Seed.
the Cryopreservation.
Tissue culture
Describes tissue and it's types. Animal tissue culture their procedure and applications. Plant tissue culture their procedure and applications.
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Plant tissue culture part 2
- 1. Plant tissue culture part-2 By: Jenifermatheew B.tech biotechnology
- 2. Methods of tissue culture : 1. Formulation of culture medium: Plant cell and tissue require a proper nutrient medium for their growth and development .the medium must contain the following components ; 1. A carbon source - sucrose . 2. Macronutrients - nitrogen, phosphorous, potassium,magnesium . 3. Micronutrients - iron, zinc, copper and chlorine. 4. Organic supplements - coconut milk, tomato, potato extract or yeast extract . 5. Hormones - IAA, NAA, 2,4-D and kinetin . pH 5.5 and 5.8 suitable for cell growth .
- 3. Collection of explant materials : An excised portion of plant body from which explants are taken from callus culture is called explant material . The following portions may be used as explant materials : 1. tap root ( eg ; carrot) 2. Stem (teak) 3. Leaf ( tobacco) 4. Meristem ( potato) 5. Embryo ( coconut)
- 4. Surface sterilization of explant materials : The process of killing microbial contaminants on the surface of explants materials is known as surface sterilization . 1. The explants materials is cleaned well with running water . 2. It is then transferred to a vial containing detergents like tween- 80 and the vial is shaken two or three times 3. Thereafter, it is kept dipped in 70% ethyl alcohol for 5 minutes . 4. It is again washed with distilled water to remove the ethonal . 5. It is then submerged in 1% of mercurine chloride solution for 10 minutes . 6. After this the plant materials is washed well distilled water.
- 5. continue... 7. Now the explant materials is free from microbial contaminants and growth inhibiting chemicals . Inoculation of explants : ● The process of transferring ex-plant to culture medium under aseptic conditions, is called inoculation . ● The sterile cabinet is properly sterilized by using ethanol and uv lamp . ● All glassware are sterilized by autoclaving and brought to the cabinet .
- 6. Incubation of explants : The explant inoculated flasks are kept in a constant temperature room or incubator for a considerable time. The process is called incubation . The temperature inside the incubator asdjusted to 25~ 2 `c which provides a constant temperature around 25`c . Cells of the explants divide repeatedly and grow into mass of parenchyma cells .the mass of activity dividing parenchymatous cells is called callus . Callus is irregular in shape due to irregular proliferation of explants cells . Its cell are closely arranged without intercellular spaces . in most cases, explant produce callus within 3-8 days.
- 8. Hardening : Gradual exposure of plantlets to outdoor environment for acclimatization is called hardening . It enables the platelets to tolerate the natural environment in which they have to be grown . There are three steps of hardening are : 1. First stage hardening . 2. Second stage hardening . 3. Third stage hardening . After hardening , plants are transplanted in the main field usual.