This document discusses four methods for long-term preservation of microbes:
1) Mineral oil or liquid paraffin storage, which can preserve bacteria for 7-15 years by limiting oxygen exposure.
2) Lyophilization, which removes water from bacterial cells through sublimation under vacuum, allowing preservation for 30 years.
3) Cryopreservation in liquid nitrogen, which preserves cells for 10-30 years when frozen at -150°C with cryoprotectants.
4) Storage in sterile soil, which can maintain viability of sporulating microorganisms like penicillium for 70-80 years.
Pure culture preservation of microbes are described in detain. Different short and long term preservation are explained in detail. Methods like Agar slant cultures (Sub culturing) & Refrigeration , Mineral Oil or Liquid Paraffin Method,Saline suspension storage, Drying in Vacuum, Storage at low temperatures (Cryopreservation) and Lyophilization (Freeze drying) are included.
Preservation of industrially important microorganisms, methods of preservation, periodic transfer, storage in saline suspension, storage in sterile soil, cryopreservation
Pure culture preservation of microbes are described in detain. Different short and long term preservation are explained in detail. Methods like Agar slant cultures (Sub culturing) & Refrigeration , Mineral Oil or Liquid Paraffin Method,Saline suspension storage, Drying in Vacuum, Storage at low temperatures (Cryopreservation) and Lyophilization (Freeze drying) are included.
Preservation of industrially important microorganisms, methods of preservation, periodic transfer, storage in saline suspension, storage in sterile soil, cryopreservation
According to Lokesh, the goal of pure culture preservation is to preserve genetic stability and microbiological viability by use of several methods. Important techniques include the straightforward and inexpensive short-term storage of refrigeration at 4°C on agar slants; the long-term preservation of deep freezing at -70°C to -80°C with cryoprotectants like glycerol or DMSO, which guarantees little genetic changes over years; and the freeze-drying process, or lyophilization, in which cultures are frozen and dehydrated under a vacuum to preserve viability and stability over long periods of time. Research, clinical diagnostics, and industrial applications all depend on these techniques to guarantee the availability of clean microbial cultures when required.
Until two decades ago the genetic resources were getting depleted owing to the
It was imperative therefore that many of the elite, economically important and endangered species are preserved to make them available when needed.
The conventional methods of storage failed to prevent losses caused due to various reasons.
A new methodology had to be devised for long term preservation of material.
Cryopreservation Prepared by Md. Ali HaidarAli Haidar
I am Md. Ali Haidar student at faculty of Agriculture, EXIM Bank Agricultural University Bangladesh. I am a future Agriculturist. I published my Presentation for helping other student.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
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A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
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3. Long term preservation
1. Preservation by overlaying culture with mineral oil or liquid parafin
2. Lyophilization
3. Preservation by liquid Nitrogen (Cryopreservation)
4. Storage in sterile soil
4. Mineral oil or liquid Paraffin storage
Many bacteria can be successfully preserved by covering the growth on an
agar slant with sterile mineral oil.
The oil must cover the slant completely. The oil should be about 1/2 in above
the tip of the slanted surface.
This can be stored at room temperature or 0-5c.
It limits the oxygen and reduce metabolism.
6. Lyophilization
Most bacteria die if cultures are allowed to become dry, although spore- and cyst-
formers can remain viable for many years.
It is vacuum sublimation technique.
In this process a dense cell suspension is placed
in small vials and frozen at —60 to - 76°C.
7. The vials are then connected to a high-vacuum line. The ice present in the frozen
suspension sublimes under the vacuum.
8. This results in dehydration of the bacteria
with a minimum of damage to delicate cell
structure.
The vials are then sealed off under a vacuum
and stored in a refrigerator.
Many species can remain viable for 30 years.
9. Preservation by liquid Nitrogen (Cryopreservation)
In this procedure cells are prepared as a dense suspension in a medium
containing a cryoprotective agent such as glycerol or dimethyl sulfoxide
(DM50). Which prevents cell damage due to ice crystal formation.
The cell suspension is sealed into small ampoules or vials and then frozen at
a controlled rate to - 150°C.
Bacterial species can remind viable for 10-30 years.
10.
11. Storage in sterile soil
It is mainly applied to sporulating microorganism e.g Pencilium
Soil storage involves inoculation of 1ml of spore suspension into soil (autoclaved
twice) and incubated at room temperature for 5-10 days.
The initial growth periods allows fungus to use the available moisture and then to
become dormant than stored to refrigerator.
Viability of organisms found to be 70-80 years.
