This document provides an overview and details of new laboratory products from YSI including the TruLab line of analog benchtop instruments for pH, ORP, and ion-selective electrode (ISE) measurements, the MultiLab line of digital multiparameter benchtop instruments, and new TruLine ISE electrodes. The TruLab and MultiLab instruments cover a range of models from single to dual or triple channel with features like memory, data export, and printer options. The document also describes the technology, measurement characteristics, and applications of 16 newly available ISE electrodes from YSI's TruLine product line.

1. Overview of New YSI Laboratory Products
Trulab, MultiLab, Ion Selective Electrodes (ISEs)
August 12, 2014
Chris Cushman, Assistant Product Manager ccushman@ysi.com
Laura St. Pierre,
Senior Product Manager
lstpierre@ysi.com

2. Outline
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TruLab
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Analog pH/mV(ORP)/ISE benchtop instruments
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MultiLab
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Digital multiparameter benchtop instruments
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New TruLine ISEs

3. TruLab: Analog pH/mV(ORP)/ISE Benchtop Instruments with new suite of pH electrodes

4. YSI TruLab Product Line
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Analog line of pH, mV and ISE instruments – released March 2014
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Specialty line of pH and ISE probes for various applications
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Positioned to compete against Thermo and Hanna
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Complimentary to MultiLab products

5. YSI TruLab Instruments
TruLab pH 1110
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Single channel analog instrument
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pH, mV(ORP), Temp
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No memory
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Segmented display
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3 stored buffer sets
TruLab pH 1310
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Single channel analog instrument
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pH, mV(ORP), Temp
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Memory (500 manual, 5000 auto)
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USB output; data importer software
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22 stored pH buffer sets
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Backlit graphic display
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Printer option (1310P)

6. YSI TruLab Instruments
TruLab pH/ISE 1320
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Dual channel analog instrument
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pH, mV(ORP), Temp, ISEs
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Connect 2 ISEs simultaneously
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Direct ion concentration readout for:
Ag+, Br-, Ca2+, Cd2+, Cl-, Cu2+, CN-, F-, I-, K+, Na+, NO3-, Pb2+, S2-, NH3, NH4+, CO2
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Memory (500 manual, 5000 auto)
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USB output; data importer software
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22 stored pH buffer sets
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Backlit graphic display
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Printer option (1320P)

7. YSI TruLab Instruments
Features of all models:
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3 year warranty
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BNC connectors
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Calibration timer
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AutoRead for repeatability
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Electrode stand included
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Universal power supply
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USB cable and PC data importer included when applicable (1310/20)
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Wide range of pH electrode options for specialized applications
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Stored buffer sets (3 for 1110, 22 for 1310/20)
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Battery or AC operation; USB power for 1310 and 1320

8. TruLine, IoLine, and ScienceLine Electrodes
TruLine: 6 single junction pH electrodes and 2 ORP electrodes
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Glass or epoxy body, refillable, and temperature/non-temp
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1 spear tip pH electrode for penetrating solids; 1 flat tip for surfaces
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Cover about 80% of pH lab applications
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Can be used with variety of meters (BNC)
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Fast response
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Refillable electrodes offer long, usable life

9. TruLine, IoLine, and ScienceLine Electrodes
IoLine: 2 triple junction glass electrodes with iodine/iodide reference
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No contamination of sample with metal ions (Tris buffers)
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3-chamber reference system allows for stability in all media and at changing temps; used in variety of applications
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1 micro version for small sample size
ScienceLine: 3 double junction electrodes with high electrolyte outflow
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Unique applications requiring particular junction
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Ground joint: highest outflow, best for solutions with a lot of solids, useful for solutions with low ions
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Platinum: high outflow to prevent clogging, but not as high as ground joint; micro size available
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Fast response; double junction reduces the chances that the reference will touch the sample, avoiding possible contamination.

10. TruLine, IoLine, and ScienceLine Electrodes
Product line features:
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German engineered/manufactured
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Variety of electrode designs to meet a large range of applications
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1 meter cable
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Longevity and fast response
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Competitive pricing
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Temperature included with all IoLine and ScienceLine, optional for TruLine
Measurement reminder:
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Refilling opening must be OPEN during calibration
and measurement

11. MultiLab: Digital Multiparameter Benchtop Instruments

12. •
4 instruments:
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4010-1(P), single channel
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4010-2, dual channel
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4010-3, triple channel
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All instruments include:
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3-year warranty!
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MultiLab importer PC software
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universal power supply
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USB cable
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electrode stand
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All instruments work with BOD Analyst Pro
YSI MultiLab Product Line

13. YSI MultiLab Instruments
MultiLab 4010-1
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Single channel digital instrument
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pH, mV(ORP), Temp, DO/BOD, cond.
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Data storage
-500 data sets in manual mode
-5,000 data sets in automatic logging mode
-Send data to PC (ASCII and .CSV); import directly into Excel®
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Large, easy to read backlit graphic display

14. YSI MultiLab Instruments
MultiLab 4010-2/3
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Two or three channel digital instrument
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pH, mV(ORP), Temp, DO/BOD, cond.
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ISE option with analog adapter
-Covers one digital input
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Data storage
-500 data sets in manual mode
-10,000 data sets in automatic logging mode
-Send data to PC, USB stick, or USB printer
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Large, easy to read color display
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Antibacterial keypad

15. •
Digital sensor recognition, processing and data transfer
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Plug-and-play connectivity; probe auto- recognized
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Sensors store unique ID with serial number and calibration status
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Monitors sensor performance and calibration to notify when it is time to change sensor cap or refill/replace pH sensor
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Includes pH, ORP, conductivity and optical based self-stirring BOD
YSI MultiLab Intelligent Digital Sensors (IDS)

16. Ion Selective Electrodes: YSI TruLine ISEs

17. Measurement Basics: Nernst Equation
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ISEs measure ion activity (i.e. “effective concentration”)
-Ion activity < actual ion concentration due to interaction of ions in solution, reduces ions available to react
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ISEs are designed to measure specific ions, but other ions can interfere
-Adding ionic strength adjustor acts to minimize/mask effect of interfering ions
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pH electrode is a Hydrogen ISE
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ISE output is in mV; follows Nernst Equation
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Slope of electrode: 2.303∗ 푅푅푅푅 푛푛푛푛
-Divalent (e.g. Ca2+) ~ 29 mV/decade (typical 26-29)
-Must have decade (i.e. order of magnitude) difference in calibration standards; can “fill-in” between standards if many used
푆푆푆푆푆푆푆푆푆푆 Ca2+=2.303∗ 8.314 퐽퐽 퐾퐾 푚푚푚∗298.15 퐾퐾∗ 12∗ 푚푚푚9.649∗104 퐶퐶 =2.958∗10−2 퐽퐽 퐶퐶−1=29.58 푚푚푚푚

18. ISE Electrode Types
Half cell ISEs:
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Contain only the ion sensor (half cell); separate reference must be used
-Both have ion exchange with the sample
-mV output determined by difference in potential b/w the sensing half cell and the reference
Combination ISEs:
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Contain the sensing electrode and reference electrode in the same body
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Much easier to use; preferred by most customers
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YSI TruLine electrodes

19. Measurement Methods
Direct measurement:
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Simple and most commonly used ISE method
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Measure mV output in samples and determine concentration based on calibration (i.e. just as is done with pH)
Incremental methods:
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Standard addition, standard subtraction, sample addition, sample subtraction
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Methods of spiking the sample
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Sample ion concentration calculated from change in potential
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YSI TruLab 1320 and MultiLab 4010-2/3 have all incremental methods preprogrammed

20. Currently Available ISEs: YSI and Competition
YSI
Brand A
Brand B
Brand C
NH3
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NH4+
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Br-
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Cd2+
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Ca2+
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Cl-
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Cu2+
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CN-
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F-
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I-
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Pb2+
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NO3-
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K+
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Ag+/S2-
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Na+
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21. •
16 parameters
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All electrodes are refillable and include double-junction reference
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4 sensor technologies
-Gas sensing, polymer/PVC membrane, solid state, glass sensor
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Included with electrode purchase:
-Unfilled electrode
-1 replacement sensor module for polymer/PVC type electrodes due to shorter shelf life
-Reference fill solution, ionic strength adjustor (ISA), and small bottle of standard
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9-12 months warranty depending on ISE
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Made in the U.S.A.
YSI TruLine ISE Electrodes

22. •
Technology: Gas-sensing
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Features: Replaceable Membrane Modules, pH sensor inside
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Range: 0.02-17,000 mg/L
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Slope: 54-59 mv/decade
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pH range: Must be 11 or higher to make NH3 gaseous. ISA has blue color at this pH
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Interferences: few, volatile amines
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Applications/Notes: Popular for wastewater, aquaculture, agriculture, industrial. pH shift caused by ammonia gas is correlated to concentration
Ammonia (NH3)

23. •Technology: Polymer/PVC Membrane
•Features: Refillable, Replaceable Sensor Modules
•Range: 0.02-1,800 mg/L
•Slope: 54-59 mv/decade
•pH range: 4-10
•Interferences: few, Na+, K+
•Applications/Notes: Popular for wastewater, environmental, industrial, food/beverage
Ammonium (NH4+)

24. •Technology: Solid State
•Features: Refillable, non-replaceable sensor
•Range: 0.4-79,900 mg/L
•Slope: 54-59 mv/decade
•pH range: <12
•Interferences: I-, Cl-, CN-, NH3-
•Applications/Notes: Pool & spa, industrial
Bromide (Br-)

25. •
Technology: Solid State
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Features: Refillable, non-replaceable sensor
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Range: 0.1-11,200 mg/L
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Slope: 26-29 mv/decade
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pH range: 2-8
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Interferences: Hg2+, Ag+, Cu2+, Pb2+
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Applications/Notes: Industrial, environmental
Cadmium (Cd2+)

26. •
Technology: Polymer/PVC Membrane
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Features: Refillable, Replaceable Sensor Modules
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Range: 0.02-40,000 mg/L
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Slope: 25-29 mv/decade
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pH range: 2.5-11
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Interferences: Pb2+, Hg2+, Si2+, Mg2+, Cu2+, K+, Tris+, Na+, others
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Applications/Notes: Popular for Aquarium, industrial, food/beverage
Calcium (Ca2+)

27. •
Technology: Solid State
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Features: Refillable, non-replaceable sensor
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Range: 1.8-35,500 mg/L
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Slope: 54-59 mv/decade
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pH range: 2-12
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Interferences: CN-, Br-, I-, OH-, S2-, NH3
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Applications/Notes: Very popular for industrial, wastewater, drinking water, food/beverage, environmental. This sensor is slightly photosensitive – store in dark place when not in use.
Chloride (Cl-)

28. •
Technology: Solid State
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Features: Refillable, non-replaceable sensor
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Range: 0.6-6,400 mg/L
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Slope: 26-29 mv/decade
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pH range: 2-6
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Interferences: Hg2+, Ag+, Fe2+, Br-, Cl-
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Applications/Notes: Industrial, environmental. Also known as Cupric ISE.
Copper (Cu2+)

29. •
Technology: Solid State
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Features: Refillable, non-replaceable sensor
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Range: 0.2-260 mg/L
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Slope: 54-59 mv/decade
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pH range: 10-14
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Interferences: I-, Br-, Cl-, S2-
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Applications/Notes: Industrial, environmental. Standard solutions not included in electrode package (safety issue)
Cyanide (CN-)

30. •
Technology: Solid State Crystal
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Features: Refillable, non-replaceable sensor
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Range: 0.02 mg/L - saturation
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Slope: 54-59 mv/decade
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pH range: 5-7 at low range, 11 at higher range
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Interferences: OH-
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Applications/Notes: Very popular for drinking water, industrial. Lanthanum fluoride crystal is the sensor.
Fluoride (F-)

31. •
Technology: Solid State
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Features: Refillable, non-replaceable sensor
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Range: 0.006-127,000 mg/L
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Slope: 54-59 mv/decade
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pH range: <14
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Interferences: CN-, Cl-, S2O32-, S2-, NH3
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Applications/Notes: Drinking water, industrial, food/beverage
Iodide (I-)

32. •
Technology: Solid State
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Features: Refillable, non-replaceable sensor
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Range: 0.02-20,700 mg/L
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Slope: 26-30 mv/decade
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pH range: 4-7
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Interferences: Hg2+, Ag+, Cu2+, Fe2+, Cd2+
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Applications/Notes: Industrial, environmental
Lead (Pb2+)

33. •
Technology: Polymer/PVC Membrane
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Features: Refillable, Replaceable Sensor Modules
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Range: 0.4-62,000 mg/L
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Slope: 54-59 mv/decade
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pH range: 2.5-11
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Interferences: few, ClO4-, ClO3-, I-, F-
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Applications/Notes: Very popular for environmental, industrial, agriculture, food/beverage
Nitrate (NO3-)

34. •
Technology: Polymer/PVC Membrane
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Features: Refillable, Replaceable Sensor Modules
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Range: 0.04-39,000 mg/L
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Slope: 54-59 mv/decade
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pH range: 2-12
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Interferences: Cs+, NH4+, Tl+, H+, Ag+,Tris+, Na+, Li+
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Applications/Notes: Popular for agriculture, food/beverage, environmental, industrial
Potassium (K+)

35. •
Technology: Solid State
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Features: Refillable, non-replaceable sensor
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Range: 0.01-107,900 mg/L (Silver) 0.003-32,100 mg/L (Sulfide)
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Slope: 54-59 mv/decade (Silver) 26-29 mv/decade (Sulfide)
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pH range: 2-12
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Interferences: Hg2+
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Applications/Notes: Industrial, environmental, food/beverage. Sulfide does not come with standard solution (stability)
Silver/Sulfide (Ag+/S2-)

36. •
Technology: Glass Sensor
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Features: Refillable, non-replaceable sensor
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Range: 0.1-23,000 mg/L
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Slope: 54-59 mv/decade
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pH range: >9
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Interferences: H+, K+
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Applications/Notes: Industrial, agriculture, power, food/beverage. Looks like a pH electrode!
Sodium (Na+)

37. TruLine ISE Tips
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Takes ~1-2 mL to fill electrode
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One order of magnitude (i.e. decade) difference between two standards; can include standards between
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Add ISA to both samples and standards to ensure similar ionic strength
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Short term (1-3 day) storage: place in low standard and fill the reference
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Long term: store dry (i.e. drain reference) and avoid sunlight
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Fill hole should be open during calibration and measurement, closed during storage

38. Thank you for attending 1-937-767-7241 ccushman@ysi.com lstpierre@ysi.com