This webinar presentation provides a foundational understanding of YSI Total Algae sensors, including how to calibrate them, which units to use, and how to interpret data gathered with the sensors. This webinar will be especially useful for new users and users transitioning from our legacy 6-series to our EXO and ProDSS platforms.
With the webinar presentation, and you’ll learn:
• Using algal pigments for early HAB detection
• 6-series chlorophyll and BGA vs. the new Total Algae (TAL) sensors
• Calibration with Rhadamine WT
• Choosing the right units
• The new cells/mL tool in KorEXO software
• Real-world data examples and challenges
Interested in total algae sampling? Check out the total algae sampling package at: https://www.ysi.com/prodss/tap-pc
Continuous Monitoring of Harmful Algal Blooms | YSIXylem Inc.
Harmful Algal Blooms (HABs) are a real problem for source water managers. Understanding what this problem is, being aware of when the problem exists and then being able to act on the problem before it is unmanageable is critical to the health of our water bodies.
DR. Stephanie Smith, YSI Product Manager, is an expert on the issue, and here she shares some of her extensive knowledge regarding HABs, and some tips for how to proactively manage them. This is her presentation given at American Water Works Association.
Monitoring for Harmful Algal Blooms | From Data to DecisionsXylem Inc.
Harmful algal blooms (HABs) have increased in both frequency and intensity in the last two decades, driven by climate change and activities that introduce growth-stimulating nutrients into waterways.
>>> Questions? Ask away: https://www.ysi.com/customer-support/ask-a-question
Concerns related to freshwater HABs range from the innocuous but unpleasant taste and odor compounds that find their way into drinking water, to fish kills and algal toxins that are harmful to human health. These consequences have increased the pressure upon researchers, watershed managers, water utility operators and public health professionals to closely monitor for the onset of HABs and prepare to respond.
Dr. Stephanie A. Smith provides a basic overview of bloom-forming algae, including a review of the toxins that are of human health concern.
You'll learn:
Which factors contribute to HABs and what makes HABs problematic
How biological, water quality and metrological parameters can be monitored by responders to minimize the impact of HABs and human exposure
How unique pigments of algae allow for monitoring using fluorescence-based tools, ranging from handheld meters to satellites
The importance of monitoring water quality parameters that either lead to or result from HABs
As the bioreporters we would like to use to detect environmental arsenic is a genetically modified bacteria, we proposed a mode of using this bacteria in the field and to discuss the safety issues with the Swiss governmental agency responsible for the environment.
Functionalised Nanoporous Materials with Direct Optical Transduction for Micr...Pierre R. Marcoux
March 7th 2011
HYBRID MATERIALS 2011: Second International Conference on Multifunctional, Hybrid and Nanomaterials; 6-10 March 2011 Strasbourg (France).
Sabine Crunaire (1), Thu-Hoa Tran-Thi (1), Khanh-Quyen Ngo (1), Pierre R. Marcoux (2), Jean-Pierre Moy (2) and Frédéric Mallard (3)
1 Laboratoire Francis Perrin, CEA/DSM/IRAMIS/SPAM – CNRS URA 2453, Gif-sur-Yvette, France.
2 Commissariat à l'Energie Atomique (CEA), LETI, MINATEC, Grenoble, France.
3 bioMérieux, Grenoble, France.
There is a well-established and growing interest in the detection and identification of microorganisms by measuring their release of volatile organic compounds (VOCs). Indeed, the measurement of the VOCs emitted by in vitro or in vivo bacterial culture could be used as a characteristic fingerprint for detection and identification. To be of greatest diagnostic value, real-time non-invasive measurements of breath or headspaces above urine, feces, blood, or sputum would replace time-consuming culture techniques.
Our study deals with the detection of microbial VOCs with functionalised nanoporous materials. These sol-gel materials include a probe molecule. This probe is chosen in order to react specifically with a target VOC, in liquid or gas phase, so as to produce an absorbent and/or fluorescent molecule within pores. This transduction pathway is called direct optical transduction.
We will focus on the detection of indole. This volatile metabolite comes out of the degradation of the amino acid tryptophan and its presence is tested in numerous identification schemes, especially to presumptively identify Escherichia coli, the gram-negative bacillus most encountered in diagnostic bacteriology.
We have prepared hybrid materials showing high-surface area (~600 m2/g), using the sol-gel chemistry. They are doped either with DMACA (dimethylaminocinnamaldehyde) or DMABA (dimethylaminobenzaldehyde), two probes reacting with indole in a complete and fast way. Therefore, we can measure indole concentration in a few minutes, by following the absorption kinetics at a chosen wavelength. Indole production of model strains of E. coli and H. alvei has also been monitored with our detectors, in liquid as well as in gas phase. We have proved the ability of these detectors to discriminate indole-producing bacteria from the others.
Continuous Monitoring of Harmful Algal Blooms | YSIXylem Inc.
Harmful Algal Blooms (HABs) are a real problem for source water managers. Understanding what this problem is, being aware of when the problem exists and then being able to act on the problem before it is unmanageable is critical to the health of our water bodies.
DR. Stephanie Smith, YSI Product Manager, is an expert on the issue, and here she shares some of her extensive knowledge regarding HABs, and some tips for how to proactively manage them. This is her presentation given at American Water Works Association.
Monitoring for Harmful Algal Blooms | From Data to DecisionsXylem Inc.
Harmful algal blooms (HABs) have increased in both frequency and intensity in the last two decades, driven by climate change and activities that introduce growth-stimulating nutrients into waterways.
>>> Questions? Ask away: https://www.ysi.com/customer-support/ask-a-question
Concerns related to freshwater HABs range from the innocuous but unpleasant taste and odor compounds that find their way into drinking water, to fish kills and algal toxins that are harmful to human health. These consequences have increased the pressure upon researchers, watershed managers, water utility operators and public health professionals to closely monitor for the onset of HABs and prepare to respond.
Dr. Stephanie A. Smith provides a basic overview of bloom-forming algae, including a review of the toxins that are of human health concern.
You'll learn:
Which factors contribute to HABs and what makes HABs problematic
How biological, water quality and metrological parameters can be monitored by responders to minimize the impact of HABs and human exposure
How unique pigments of algae allow for monitoring using fluorescence-based tools, ranging from handheld meters to satellites
The importance of monitoring water quality parameters that either lead to or result from HABs
As the bioreporters we would like to use to detect environmental arsenic is a genetically modified bacteria, we proposed a mode of using this bacteria in the field and to discuss the safety issues with the Swiss governmental agency responsible for the environment.
Functionalised Nanoporous Materials with Direct Optical Transduction for Micr...Pierre R. Marcoux
March 7th 2011
HYBRID MATERIALS 2011: Second International Conference on Multifunctional, Hybrid and Nanomaterials; 6-10 March 2011 Strasbourg (France).
Sabine Crunaire (1), Thu-Hoa Tran-Thi (1), Khanh-Quyen Ngo (1), Pierre R. Marcoux (2), Jean-Pierre Moy (2) and Frédéric Mallard (3)
1 Laboratoire Francis Perrin, CEA/DSM/IRAMIS/SPAM – CNRS URA 2453, Gif-sur-Yvette, France.
2 Commissariat à l'Energie Atomique (CEA), LETI, MINATEC, Grenoble, France.
3 bioMérieux, Grenoble, France.
There is a well-established and growing interest in the detection and identification of microorganisms by measuring their release of volatile organic compounds (VOCs). Indeed, the measurement of the VOCs emitted by in vitro or in vivo bacterial culture could be used as a characteristic fingerprint for detection and identification. To be of greatest diagnostic value, real-time non-invasive measurements of breath or headspaces above urine, feces, blood, or sputum would replace time-consuming culture techniques.
Our study deals with the detection of microbial VOCs with functionalised nanoporous materials. These sol-gel materials include a probe molecule. This probe is chosen in order to react specifically with a target VOC, in liquid or gas phase, so as to produce an absorbent and/or fluorescent molecule within pores. This transduction pathway is called direct optical transduction.
We will focus on the detection of indole. This volatile metabolite comes out of the degradation of the amino acid tryptophan and its presence is tested in numerous identification schemes, especially to presumptively identify Escherichia coli, the gram-negative bacillus most encountered in diagnostic bacteriology.
We have prepared hybrid materials showing high-surface area (~600 m2/g), using the sol-gel chemistry. They are doped either with DMACA (dimethylaminocinnamaldehyde) or DMABA (dimethylaminobenzaldehyde), two probes reacting with indole in a complete and fast way. Therefore, we can measure indole concentration in a few minutes, by following the absorption kinetics at a chosen wavelength. Indole production of model strains of E. coli and H. alvei has also been monitored with our detectors, in liquid as well as in gas phase. We have proved the ability of these detectors to discriminate indole-producing bacteria from the others.
No single liquid chromatography (LC) detector delivers ideal results. Often with LC detectors one analyte responds more strongly than another, or may not respond at all. What is most desired is the ability to accurately measure a wide range of analytes with consistent response simultaneously.
Charged Aerosol detection (CAD) is a mass sensitive technique for determining levels of any non-volatile and many semi-volatile analytes after separation by liquid chromatography. This technique provides consistent analyte response independent of chemical characteristics and gives greater sensitivity over a wider dynamic range. An analytes response does not depend on optical properties, like with UV-vis absorbance, or the ability to ionize, as with mass spectrometry (MS). The presence of chromophoric groups, radiolabels, ionizable moieties, or chemical derivatization is needed for detection.
Presentation Outline for Expanding Your High Performance Liquid Chromatography and Ultra High Performance Liquid Chromatography Capabilities with Universal Detection-Shedding Light on Non-Chromophore Compounds:
• Introduction to Charged Aerosol Detection
• How Charged Aerosol Technology Works
• Comparison with Evaporative Light Scattering Detectors
(ELSD)
• Examples of Applications
• Inverse Gradient Solution for Uniform Response
Cholesterol Bio Sensors: getter better fastJeffrey Funk
These slides use concepts from my (Jeff Funk) course entitled analyzing hi-tech opportunities to analyze the increasing economic feasibility of bio-sensors for measuring cholesterol in humans. Bio-sensors detect the level of cholesterol (and other biological materials) using enzymes, matrices, and transducers. The enzymes, which are held in a matrix, react with the cholesterol and an electric signal is produced from an amperometric transducer. Improvements in sensitivity, response time, shelf life, detection limit, and reusability have been achieved through creating more appropriate biological materials for the enzymes, matrices, and transducers.
The RA802 Pharmaceutical Analyser combines Renishaw's proprietary LiveTrackTM and StreamLineTM technologies to generate chemical images up to 150 times faster than conventional methods, whilst maintaining focus ? ensuring high quality pharmaceutical tablet imaging.
MicroPRO, A Rapid Microbiology Method Based on Flow Cytometryguest32bcc5
The MicroPRO is a rapid microbiology method based on flow cytometry to detect presence/absence of bacteria, yeast and molds in pharmaceutical and cosmetic products in 24 hours. It can also detect these micro-organisms quantitatively in 5 minutes in water and swabs.
HPLC stands for “High-performance liquid chromatography”(sometimes referred to as High-pressure liquid chromatography).
High performance liquid chromatography is a powerful tool in analysis, it yields high performance and high speed compared to traditional columns chromatography because of the forcibly pumped mobile phase.
It is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of a mixture.
The Future of Metabolic Phenotyping Using data bandwidth to maximize N, analy...InsideScientific
Methods matter. In metabolic measurement, confidence in reproducible results relies heavily on the design of the system used to acquire data. In the field of translational metabolic and behavioral phenotyping there is critical demand for more – throughput, standardization, synchronization of diverse data streams, temporal resolution, efficiency of workflow, and verification of results. We compare continuous and switched metabolic measurement methodologies and explore applications that benefit most from continuous measurement.
In this exclusive webinar sponsored by Sable Systems International, experts contrast methodologies and discuss how to improve best practices in metabolic phenotyping. We show how advances in high-bandwidth metabolic measurement, as implemented in Promethion metabolic phenotyping systems, leverage a 60- to 1200-fold increase in temporal resolution and achieve synchrony with intake and other behavioral data.
Key Topics:
* Time-saving methodologies for increasing throughput in multiplexed or continuous metabolic phenotyping
* Evaluation criteria for selecting a metabolic measurement system
* How the home-cage advantage of a pull-mode system reliably increases animal safety while dramatically reducing stress on both the animal and the researcher
* How to improve the resolution, accuracy and versatility of metabolic data using water vapor measurement
* The importance of raw data retention in metabolic phenotyping
* How deep data field format leads to greater traceability, improved reliability and far greater data extraction versatility to address research objectives
* How exact metabolic costs can be assigned to transient activities, with important implications for studies of energy balance, obesity, drug kinetics and metabolic diseases
An overview of Pine Lake Laboratories capabilities involving oligonucleotides. Includes challenges, examples, method development, validation, and stability!
20160219 - M. Agostini - Nuove tecnologie per lo studio del DNA tumorale libe...Roberto Scarafia
Nano Inspired Biomedicine Laboratory
1 Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Italy.
2 Istituto di Ricerca Pediatrica- Città della Speranza, Padova, Italy.
This presentation will explain to you the types of nanosensors in different fields and application of nanosensors in detail which will enhance your knowledge in the field of nanotechnology
Nitrogen Determination Made Easy with DUMATHERM Combustion AnalyzerXylem Inc.
The DUMATHERM® N-Pro Combustion Analyzer uses all of the advantages of the Dumas combustion method and enables labs to analyze a wide range of samples. DUMATHERM® reduces analysis time to 3 minutes or less and 98% time savings compared to Kjeldahl.
Learn more about our fully-automated combustion analyzer, that can deliver results within 3 minutes!
Wastewater strategies for Biological Nutrient Removal of NitrogenXylem Inc.
Biological nutrient removal (BNR) is the new standard for wastewater secondary treatment strategies. BNR involves the recruitment and growth of specific microorganisms that either convert or remove nutrients like nitrogen and phosphorus. Nitrogen removal, specifically, can take many forms and requires precise control of the environment using sensors, aeration, and chemicals for success.
In this educational webinar, our experts discuss:
- How nitrogen behaves in wastewater and why we want to remove it
- Identify the optimal conditions required for nitrogen removal in each stage of the activated sludge process
- Applications for online monitoring instrumentation to help improve the biological nutrient removal strategy
Watch the recording and get CEUs here >>> https://video.ysi.com/webinar-biological-nutrient
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Similar to Are You Ready for Harmful Algal Bloom Season? | Preparing for HAB Monitoring
No single liquid chromatography (LC) detector delivers ideal results. Often with LC detectors one analyte responds more strongly than another, or may not respond at all. What is most desired is the ability to accurately measure a wide range of analytes with consistent response simultaneously.
Charged Aerosol detection (CAD) is a mass sensitive technique for determining levels of any non-volatile and many semi-volatile analytes after separation by liquid chromatography. This technique provides consistent analyte response independent of chemical characteristics and gives greater sensitivity over a wider dynamic range. An analytes response does not depend on optical properties, like with UV-vis absorbance, or the ability to ionize, as with mass spectrometry (MS). The presence of chromophoric groups, radiolabels, ionizable moieties, or chemical derivatization is needed for detection.
Presentation Outline for Expanding Your High Performance Liquid Chromatography and Ultra High Performance Liquid Chromatography Capabilities with Universal Detection-Shedding Light on Non-Chromophore Compounds:
• Introduction to Charged Aerosol Detection
• How Charged Aerosol Technology Works
• Comparison with Evaporative Light Scattering Detectors
(ELSD)
• Examples of Applications
• Inverse Gradient Solution for Uniform Response
Cholesterol Bio Sensors: getter better fastJeffrey Funk
These slides use concepts from my (Jeff Funk) course entitled analyzing hi-tech opportunities to analyze the increasing economic feasibility of bio-sensors for measuring cholesterol in humans. Bio-sensors detect the level of cholesterol (and other biological materials) using enzymes, matrices, and transducers. The enzymes, which are held in a matrix, react with the cholesterol and an electric signal is produced from an amperometric transducer. Improvements in sensitivity, response time, shelf life, detection limit, and reusability have been achieved through creating more appropriate biological materials for the enzymes, matrices, and transducers.
The RA802 Pharmaceutical Analyser combines Renishaw's proprietary LiveTrackTM and StreamLineTM technologies to generate chemical images up to 150 times faster than conventional methods, whilst maintaining focus ? ensuring high quality pharmaceutical tablet imaging.
MicroPRO, A Rapid Microbiology Method Based on Flow Cytometryguest32bcc5
The MicroPRO is a rapid microbiology method based on flow cytometry to detect presence/absence of bacteria, yeast and molds in pharmaceutical and cosmetic products in 24 hours. It can also detect these micro-organisms quantitatively in 5 minutes in water and swabs.
HPLC stands for “High-performance liquid chromatography”(sometimes referred to as High-pressure liquid chromatography).
High performance liquid chromatography is a powerful tool in analysis, it yields high performance and high speed compared to traditional columns chromatography because of the forcibly pumped mobile phase.
It is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of a mixture.
The Future of Metabolic Phenotyping Using data bandwidth to maximize N, analy...InsideScientific
Methods matter. In metabolic measurement, confidence in reproducible results relies heavily on the design of the system used to acquire data. In the field of translational metabolic and behavioral phenotyping there is critical demand for more – throughput, standardization, synchronization of diverse data streams, temporal resolution, efficiency of workflow, and verification of results. We compare continuous and switched metabolic measurement methodologies and explore applications that benefit most from continuous measurement.
In this exclusive webinar sponsored by Sable Systems International, experts contrast methodologies and discuss how to improve best practices in metabolic phenotyping. We show how advances in high-bandwidth metabolic measurement, as implemented in Promethion metabolic phenotyping systems, leverage a 60- to 1200-fold increase in temporal resolution and achieve synchrony with intake and other behavioral data.
Key Topics:
* Time-saving methodologies for increasing throughput in multiplexed or continuous metabolic phenotyping
* Evaluation criteria for selecting a metabolic measurement system
* How the home-cage advantage of a pull-mode system reliably increases animal safety while dramatically reducing stress on both the animal and the researcher
* How to improve the resolution, accuracy and versatility of metabolic data using water vapor measurement
* The importance of raw data retention in metabolic phenotyping
* How deep data field format leads to greater traceability, improved reliability and far greater data extraction versatility to address research objectives
* How exact metabolic costs can be assigned to transient activities, with important implications for studies of energy balance, obesity, drug kinetics and metabolic diseases
An overview of Pine Lake Laboratories capabilities involving oligonucleotides. Includes challenges, examples, method development, validation, and stability!
20160219 - M. Agostini - Nuove tecnologie per lo studio del DNA tumorale libe...Roberto Scarafia
Nano Inspired Biomedicine Laboratory
1 Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Italy.
2 Istituto di Ricerca Pediatrica- Città della Speranza, Padova, Italy.
This presentation will explain to you the types of nanosensors in different fields and application of nanosensors in detail which will enhance your knowledge in the field of nanotechnology
Nitrogen Determination Made Easy with DUMATHERM Combustion AnalyzerXylem Inc.
The DUMATHERM® N-Pro Combustion Analyzer uses all of the advantages of the Dumas combustion method and enables labs to analyze a wide range of samples. DUMATHERM® reduces analysis time to 3 minutes or less and 98% time savings compared to Kjeldahl.
Learn more about our fully-automated combustion analyzer, that can deliver results within 3 minutes!
Wastewater strategies for Biological Nutrient Removal of NitrogenXylem Inc.
Biological nutrient removal (BNR) is the new standard for wastewater secondary treatment strategies. BNR involves the recruitment and growth of specific microorganisms that either convert or remove nutrients like nitrogen and phosphorus. Nitrogen removal, specifically, can take many forms and requires precise control of the environment using sensors, aeration, and chemicals for success.
In this educational webinar, our experts discuss:
- How nitrogen behaves in wastewater and why we want to remove it
- Identify the optimal conditions required for nitrogen removal in each stage of the activated sludge process
- Applications for online monitoring instrumentation to help improve the biological nutrient removal strategy
Watch the recording and get CEUs here >>> https://video.ysi.com/webinar-biological-nutrient
Mission: Magazine, Issue #6 - The Magazine that Addresses Critical Water IssuesXylem Inc.
Mission: Water is a complimentary magazine featuring the world's most current water issues and how people, like you, are tackling these ever-important challenges. Our mission is to share inspirational stories of determination, curiosity and discovery – and how great advancements are being made to better understand and protect our vital water resources.
This issue includes flood protection, harmful algal blooms, and red tide monitoring.
https://www.ysi.com/mission-water
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Mission: Water is a complimentary magazine featuring the world's most current water issues and how people, like you, are tackling these ever-important challenges. Our mission is to share inspirational stories of determination, curiosity and discovery – and how great advancements are being made to better understand and protect our vital water resources.
This issue includes climate modelling, legendary rivers, and decades of scientific discovery.
https://www.ysi.com/mission-water
Phosphorus Removal Essentials in wastewater | YSI WebinarXylem Inc.
Are you facing challenges with lower effluent phosphorus limits at your WRRF? YSI experts review phosphorus removal strategies in municipal wastewater applications.
Phosphorus, primarily existing as phosphate, is a nutrient of concern for many wastewater operators. Effluent phosphorus limits continue to be lowered to protect our lakes and rivers from eutrophication. To meet these limits, operators need to improve treatment processes to remove phosphorus as efficiently as possible.
Orthophosphate Analyzer for Wastewater Monitoring | YSI IQ SensorNet AlyzaXylem Inc.
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Applications include:
Monitoring and control of chemical phosphorus removal, reducing dosing chemicals and sludge production
Effluent monitoring to ensure compliance
Bringing GOES Transmitters Back to the Future | YSI WebinarXylem Inc.
Our goal for this webinar is to give you a better understanding of the GOES transmitter interruptions that occurred on October 21, 2018 and caused data loggers to think it was 1963.
We provide an in-depth technical description of what caused the anomaly, how it was resolved, and the tools and people that were deployed to support our customers. This includes a full view of the timeline of events, a talk from YSI's R&D Director Steve Parmley as he describes GPS clock rollover, and a look at what the future holds for ongoing customer support and the GOES transmitters themselves.
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Automation is a disrupting force throughout the world, impacting our lives and creating a new normal for generations to come.
Join industry experts Kevin Simpson, Geoff Douglass, and Rob Porrett as they discuss automated vehicles for monitoring water quality and quantity – and the impact on the environmental community.
In this webinar, we provide a foundational understanding of Autonomous Surface Vehicles (ASV), share our top tips for better water monitoring, discuss the impact autonomous vehicles have on the environmental community, and introduce our latest and greatest water monitoring technology.
Watch this webinar to learn:
• Top tips to improve your water quality monitoring
• Best applications for an Autonomous Surface Vehicles (ASV)
• Real-world data examples and challenges
• A peek at our newest ASV technology—HYCAT
• And more!
Mission: Magazine, Issue #3 - The Magazine that Addresses Critical Water IssuesXylem Inc.
Mission: Water is a complimentary magazine featuring the world's most current water issues and how people, like you, are tackling these ever-important challenges. Our mission is to share inspirational stories of determination, curiosity and discovery – and how great advancements are being made to better understand and protect our vital water resources.
https://www.ysi.com/mission-water
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Mission: Water is a complimentary magazine featuring the world's most current water issues and how people, like you, are tackling these ever-important challenges. Our mission is to share inspirational stories of determination, curiosity and discovery – and how great advancements are being made to better understand and protect our vital water resources.
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Mission: Water is a complimentary magazine featuring the world's most current water issues and how people, like you, are tackling these ever-important challenges. Our mission is to share inspirational stories of determination, curiosity and discovery – and how great advancements are being made to better understand and protect our vital water resources.
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https://www.ysi.com/iqsn2020
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Precise aeration control with continuous monitoring of dissolved oxygen
Sludge wasting control to ensure an adequate biomass population
Chemical dosing control for accurate dosing amounts, every time
Find out more about how these facilities are using the IQ SensorNet system to monitor and control their wastewater process to meet their unique, individual plant needs and see how you can use these same controls at your facility.
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See how several aquaculture facilities are using continuous monitoring and control to improve operational efficiency
Specific details on the facility's design and operation
Implementation of the monitoring and control system including setpoints, sensor location, and other critical control parameters
Dissolved oxygen monitoring and feeder control
Installation and set-up overview
Followed by a brief Q&A session
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The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
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Exposé invité Journées Nationales du GDR GPL 2024
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Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
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Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Nucleophilic Addition of carbonyl compounds.pptxSSR02
Nucleophilic addition is the most important reaction of carbonyls. Not just aldehydes and ketones, but also carboxylic acid derivatives in general.
Carbonyls undergo addition reactions with a large range of nucleophiles.
Comparing the relative basicity of the nucleophile and the product is extremely helpful in determining how reversible the addition reaction is. Reactions with Grignards and hydrides are irreversible. Reactions with weak bases like halides and carboxylates generally don’t happen.
Electronic effects (inductive effects, electron donation) have a large impact on reactivity.
Large groups adjacent to the carbonyl will slow the rate of reaction.
Neutral nucleophiles can also add to carbonyls, although their additions are generally slower and more reversible. Acid catalysis is sometimes employed to increase the rate of addition.
BREEDING METHODS FOR DISEASE RESISTANCE.pptxRASHMI M G
Plant breeding for disease resistance is a strategy to reduce crop losses caused by disease. Plants have an innate immune system that allows them to recognize pathogens and provide resistance. However, breeding for long-lasting resistance often involves combining multiple resistance genes
2. Data to Decisions Webinar, Part I
2
http://video.ysi.com/ysi-webinar-monitoring-for-harmful-algal-blooms
3. Data to Decisions Webinar, Part II
3
http://video.ysi.com/ysi-webinar-drowning-in-data-monitoring-harmful
4. Dr. Stephanie A. Smith
4
BACKGROUND
Ph.D. in Microbiology
The Ohio State University
Assistant Professor
Senior Scientist
Entrepreneur
Product Manager
7. Why Pigments?
Why algae love them
• Harvest light for photosynthesis
• Can regulate pigment levels
7
8. Why Pigments?
Why algae love them
• Harvest light for photosynthesis
• Can regulate pigment levels
Why we love them
• Fluorescent molecules that we can detect in situ
8
Excitation
• Absorbs light energy of a specific
wavelength
Emission
• Releases light of a longer wavelength,
but lower energy
9. Why Pigments?
Why algae love them
• Harvest light for photosynthesis
• Can regulate pigment levels
Why we love them
• Fluorescent molecules that we can detect in situ
Why you should love them, too
• Early detection of HABs
9
Monitor Manage
10. Which Pigments?
Chlorophyll
• All algae
Phycocyanin
• Blue-green algae native to freshwater
Phycoerythrin
• Blue-green algae native to marine water
10
15. EXO and ProDSS Total Algae Sensors
15
• EXO
• TAL-PC (599102-01) and TAL-PE
(599103-01)
• ProDSS
• TAL-PC (626210) and TAL-PE
(626211)
Spot
Sampling
Continuous
Monitoring
16. EXO and ProDSS Sensors
16
Solid—excitation
Dotted--emission
Fluorescence Resonance Energy Transfer (FRET)
17. EXO and ProDSS Sensors
17
Parameter Chlorophyll TAL-PC TAL-PE
Ex ʎ 470 ± 15 nm 590 ± 15 nm 525 ± 15 nm
Em ʎ (meas.) 685 ± 20 nm 685 ± 20 nm 685 ± 20 nm
Range 0 to 100 RFU;
0 to 400 μg/L Chl
0 to 100 RFU;
0 to 100 μg/L PC
0 to 100 RFU;
0 to 280 μg/L PE
Resolution 0.01 RFU;
0.01 μg/L Chl
0.01 RFU;
0.01 μg/L PC
0.01 RFU;
0.01 μg/L PE
Detection Limit 0.01 μg/L Chl 0.01 μg/L PC 0.01 μg/L PE
18. EXO/ProDSS vs. 6-series Phycocyanin
18
Parameter 6-Series
BGA-PC
EXO
TAL-PC
So?
Ex LED Single Dual Only need one
sensor
Em ʎ
(meas.)
640 ± 40 nm 685 ± 20 nm Less
interference
Range 0-280,000 cells/mL
0 to 100 RFU
0 to 100 μg/L:
0 to 100 RFU
Build cfu/mL in
KorEXO
Resolution 0.1 RFU;
0.1 μg/L Chl
0.01 RFU;
0.01 μg/L PC
10X better
Detection
Limit
0.1 RFU 0.01 RFU 10X better
19. Fluorescence-Based Sensor Challenges
• Scale of power output varies slightly from sensor to sensor
• RAW units delivered by an individual sensor are unique to its construction
• Sensors must be “tuned” so that they are standardized for performance specifications
• All fluorescence sensors drift
19
calibrated line
Drift A
Drift B
Drift C
[RhoWT]
RFU
• A: slope is same
• What’s the temp?
• B: slope changed, especially
affects higher readings
• C: slope changes, especially
affects lower readings
20. Fluorescence-Based Sensor Challenges
• Scale of power output varies slightly from sensor to sensor
• RAW units delivered by an individual sensor are unique to its construction
• Sensors must be “tuned” so that they are standardized for performance specifications
• All fluorescence sensors drift
20
calibrated line
Drift A
Drift B
Drift C
[RhoWT]
RFU
These realities are why sensors
must be calibrated
21. OMG—this scares me! These sensors sound crazy!
21
• These are very low-drift sensors
• How often you should calibrate depends upon
1. Which end of the line you care most about
- Re-zeroing regularly may suffice, but pay attention to
how much it changes
2. Your environment
3. Your institution’s requirements
4. Age of the sensor
Some users re-cal every 90 days, some once a
year, some once a week, some…
24. Calibration Units
• Recommended: Raw Fluorescent Units (RFU)
• Default unit
• Enables monitoring of drift and normalization of fleet of sensors
• Calibrates the 0-100% scale of the sensor’s output
• µg/L of pigment equivalents (ppb)
• Estimated concentration of chl and either PC or PE (not RhoWT!)
• Developed with laboratory cultures and extractions
• Ideally, users should check how well their site lines up with our ppb
24
25. Congratulations on your new TAL Sensor!
Your TAL sensor was calibrated in the factory, and yes, it
can be used right away. However…
• Is it going to be incorporated into a larger sensor network?
• Do you want to compare data among sensors?
• How will you know if/when it has drifted?
Recommendation: Perform a two-point calibration!
25
28. 2-point calibration
Step 1: Prepare Rhodamine WT Calibration Solution(s)
28
Kingscote
Item 106023
2.5% RhoWT
5 mL
Bring to 1000 mL
With DI water
125 mg/L
RhoWT
Bring to 1000 mL
with DI water
0.625 mg/L
RhoWT
Bring to 1000 mL
with DI water
0.025 mg/L
RhoWT
Step 1:
Prep solutions
Step 2:
Tempco values
Step 3:
Calibrate
29. 2-point calibration
Step 2: Place sensors in solution and find your temperature-
compensated values in the manual
29
Step 1:
Prep solutions
Step 2:
Tempco values
Step 3:
Calibrate
Use the reading from
the CT sensor!
Pigment µg/L ≠
RhoWT µg/L
30. 2-point calibration
Step 3: Calibrate
1. In Kor or the handheld:
• Enter temp-corrected RFU or µg/L
• Stabilize the reading
• Apply the calibration to the sensor
2. Repeat for all channels,
all units of interest
30
Step 1:
Prep solutions
Step 2:
Tempco values
Step 3:
Calibrate
31. What happened to cells/mL?
• 6-series sensors had this unit
• Some regulatory agencies still require this
unit
• Why we abandoned it: one correlation
was not reliable for all algae
• “I don’t care—it’s required where I live…”
• Coming to KorEXO in May!
31
36. Inner Filter Effect (IFE)
36
Absorbent
molecules
Light emitted
from sensor
Light from
excited Chl
absorbed by
“quenchers”
RFU
Chlorophyll
With quenchers
No quenchers
37. Temperature Effects on Fluorescence
• There is an inverse relationship
between temperature and
fluorescence
• This example: profiling from 0-
12m depth in December
• The question: is this showing a
change in algae population, or a
change in fluorescence due to
temp?
• Thank you, Jamie Carr of MA
DCR!
37
38. The Algae Don’t Care About You
• Temperature effects on membranes
• Pigment quenching
• Photobleaching, non-photochemical
• Pigment turnover
• Movement in the water column
38
39. Temperature Effects on Membranes
• In vivo, pigments are membrane-
bound
• Temperature affects membrane
fluidity, and that affects
fluorescence
• This is a different effect than the
inverse relationship between
fluorescence and temp
39
40. Temperature Effects on Membranes
Temperature effects depended upon:
• Temp the algae were grown at
• Whether you were increasing or decreasing
temperature relative to growth temperature
40
41. Pigment Turnover
41
• Non-photochemical quenching
• Regulation of intracellular pigment
concentrations
• Diurnal turnover
• Multiparameter context can help you
understand how significant this may
be, if it is at all
Aquarist Magazine & Blog
42. Movement in the water column
42
http://www1.biologie.uni-hamburg.de/b-
online/library/webb/BOT311/Cyanobacteria/
Cyanobacteria.htm
43. In spite of these challenges…
43
• Limitations of sensor construction
• Calibration
• Interferences
• Algae are mean
The most important
algae monitoring in the
world uses TAL sensors
45. Case Study: Lake Erie Monitoring
45
• 3M+ people drink Lake Erie Water
• Microcystis aeruginosa
• 2014 Toledo water crisis
• Network of monitoring buoys with EXO
• Thanks to Ed Verhamme of LimnoTech
for the data to follow!
47. Normalize your fleet!
• Co-calibration sets the same
baseline for all the sensors
• Cal checks during visits/
maintenance
47
48. Oregon, OH Pump Station, 2015
48
• Raw water intake
(not finished water!)
• EXO2 TAL-PC
• Microcystin by ELISA
• Lake Erie has fairly
consistent blooms of
M. aerugionosa
49. Raw water, Toledo Pump Station
• Value is in observing year
over year trends…
• Learn your system
• TAL-PC alone doesn’t
define treatability
49
2017
2016
50. See more data and tools!
• Portal.glos.us for ALL GLOS data
• Habs.glos.us for post-2014 Toledo water crisis
• Glbuoys.glos.us
• http://glbuoys.glos.us/eire for LE stations
• Dev.glos.us/tools/export NEW tool you can beta test!
50
51. Case Study:
Ocean Research and Conservation Association
51
http://api.kilroydata.org/public/
TAL-PE sensor on EXO
Thank you to Michael Corbet and ORCA!
55. Are you Ready for HAB Season?
Sensor Construction
• EXO and ProDSS TAL sensors are fundamentally different from 6-series sensors
• More sensitive, highly specific for pigments
• All fluorescence-based sensors drift
55
56. Are you Ready for HAB Season?
Sensor Calibration
• Rhodamine WT is a secondary calibrator
• Two-point calibrations are recommended
• “One-point calibrations” re-zero the sensors
• Calibration synchronizes a network of sensors
• Use RFU, unless you’re going to check or build
your own correlations for pigment or cells/mL
56
calibrated line
Drift A
Drift B
Drift C
[RhoWT]
RFU
57. Are you Ready for HAB Season?
Monitoring in the Real World
• Environmental interferences are possible, and multiparameter monitoring can help
you understand your risks
• Monitor for changes from a baseline in your system, and look for sustained changes
57
Turbidity
RFU
RFU
Chlorophyll
With quenchers
No quenchers