Addgene is a global nonprot plasmid repository. Over 2,000 labs have deposited plasmids to Addgene and we distribute over 100,000
plasmids per year. Thus, we are in a unique position to observe and quantify how new technologies spread through the scientic community.
We will compare global distribution data for Zinc Finger, TALE, and CRISPR plasmids over time. We’ll analyze CRISPR plasmids in more detail
by summarizing the latest plasmid developments and by examing trends in genome engineering technologies. Finally, we’ll present data
describing the research tools shared by synthetic biology labs.
An Introduction to Crispr Genome EditingChris Thorne
In this short presentation, I make a case for doing genome editing vs some of the approaches that have gone before, describe some of the tools available, and the focus on CRISPR-Cas9, what it is, where it's come from and how it works.
Edward Perello, CBO of Desktop Genetics, joins us at the Science: Disrupt London Session on Disruptor Stories to talk machine learning, CRSIPR, pivoting and his startup story.
An Introduction to Crispr Genome EditingChris Thorne
In this short presentation, I make a case for doing genome editing vs some of the approaches that have gone before, describe some of the tools available, and the focus on CRISPR-Cas9, what it is, where it's come from and how it works.
Edward Perello, CBO of Desktop Genetics, joins us at the Science: Disrupt London Session on Disruptor Stories to talk machine learning, CRSIPR, pivoting and his startup story.
Have you considered that protein over-expression or inefficient mRNA knockdown may be masking physiological effects in your assays? Increasingly scientists are moving to endogenous gene-editing to characterise the function of their genes of interest.
Dr Chris Thorne from Cambridge Biotech Horizon Discovery discusses the ground breaking gene-editing technology CRISPR. The simplicity of experimental design has led to rapid adoption of the technology across the scientific community. However, challenges remain.
This Slidedeck focuses specifically on implementing CRISPR experiments, and explore a number of key considerations crucial to maximising chances of targeting success, whether your goal is to generate a knock-out or a knock-in. Chris also takes a look at some of the alternative uses of CRISPR, including sgRNA genome wide synthetic lethality screens.
The slides aim to support those researchers either planning to or already using CRISPR gene-editing in their lab. Horizon Discovery have also recently launched a program aimed specifically at academic cell biologists to promote the adoption of CRISPR by offering FREE CRISPR Reagents for knock-out cell line generation - more information available here. http://www.horizondiscovery.com/what-we-do/discovery-toolbox/genassist-crispr--raav-genome-editing-tools
CRISPR has become an increasingly popular tool for genome editing, in part because it is highly flexible and relatively easy to implement compared to other technologies. However, for scientists beginning to work with this method, the wide range of products and variety of editing approaches can be overwhelming. In this presentation, Justin Barr provides a simple explanation of the steps for planning your experiment, including guide RNA design, an overview of delivery methods, and options for measuring editing results. He also discusses how to generate specific mutations in the genome using homology-directed repair (HDR).
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Have you considered that protein over-expression or inefficient mRNA knockdown may be masking physiological effects in your assays? Increasingly scientists are moving to endogenous gene-editing to characterise the function of their genes of interest.
Dr Chris Thorne from Cambridge Biotech Horizon Discovery discusses the ground breaking gene-editing technology CRISPR. The simplicity of experimental design has led to rapid adoption of the technology across the scientific community. However, challenges remain.
This Slidedeck focuses specifically on implementing CRISPR experiments, and explore a number of key considerations crucial to maximising chances of targeting success, whether your goal is to generate a knock-out or a knock-in. Chris also takes a look at some of the alternative uses of CRISPR, including sgRNA genome wide synthetic lethality screens.
The slides aim to support those researchers either planning to or already using CRISPR gene-editing in their lab. Horizon Discovery have also recently launched a program aimed specifically at academic cell biologists to promote the adoption of CRISPR by offering FREE CRISPR Reagents for knock-out cell line generation - more information available here. http://www.horizondiscovery.com/what-we-do/discovery-toolbox/genassist-crispr--raav-genome-editing-tools
CRISPR has become an increasingly popular tool for genome editing, in part because it is highly flexible and relatively easy to implement compared to other technologies. However, for scientists beginning to work with this method, the wide range of products and variety of editing approaches can be overwhelming. In this presentation, Justin Barr provides a simple explanation of the steps for planning your experiment, including guide RNA design, an overview of delivery methods, and options for measuring editing results. He also discusses how to generate specific mutations in the genome using homology-directed repair (HDR).
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
DERIVATION OF MODIFIED BERNOULLI EQUATION WITH VISCOUS EFFECTS AND TERMINAL V...Wasswaderrick3
In this book, we use conservation of energy techniques on a fluid element to derive the Modified Bernoulli equation of flow with viscous or friction effects. We derive the general equation of flow/ velocity and then from this we derive the Pouiselle flow equation, the transition flow equation and the turbulent flow equation. In the situations where there are no viscous effects , the equation reduces to the Bernoulli equation. From experimental results, we are able to include other terms in the Bernoulli equation. We also look at cases where pressure gradients exist. We use the Modified Bernoulli equation to derive equations of flow rate for pipes of different cross sectional areas connected together. We also extend our techniques of energy conservation to a sphere falling in a viscous medium under the effect of gravity. We demonstrate Stokes equation of terminal velocity and turbulent flow equation. We look at a way of calculating the time taken for a body to fall in a viscous medium. We also look at the general equation of terminal velocity.
Salas, V. (2024) "John of St. Thomas (Poinsot) on the Science of Sacred Theol...Studia Poinsotiana
I Introduction
II Subalternation and Theology
III Theology and Dogmatic Declarations
IV The Mixed Principles of Theology
V Virtual Revelation: The Unity of Theology
VI Theology as a Natural Science
VII Theology’s Certitude
VIII Conclusion
Notes
Bibliography
All the contents are fully attributable to the author, Doctor Victor Salas. Should you wish to get this text republished, get in touch with the author or the editorial committee of the Studia Poinsotiana. Insofar as possible, we will be happy to broker your contact.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Toxic effects of heavy metals : Lead and Arsenicsanjana502982
Heavy metals are naturally occuring metallic chemical elements that have relatively high density, and are toxic at even low concentrations. All toxic metals are termed as heavy metals irrespective of their atomic mass and density, eg. arsenic, lead, mercury, cadmium, thallium, chromium, etc.
Trends in CRISPR and Synthetic Biology Technologies at Addgene
1. Trends in CRISPR and
SynBio Technologies:
Data from Addgene’s
Plasmid Repository
Eric J. Perkins, Matthew Ferenc,
Julian Taylor-Parker, Larissa Haliw,
Zachary Tribbett, Elisha Fielding,
Pamela McA’Nulty, Joanne Kamens,
Melina Fan
Addgene, Cambridge, MA 02139
www.addgene.org help@addgene.org
2. Addgene: A Global, Nonprofit Plasmid
Repository
Addgene is in a unique position to observe and
quantify how new technologies spread through
the scientic community.
• Over 2,000 labs have deposited plasmids to Addgene
• Addgene distributes over 100,000 plasmids per year.
We will compare global distribution data for Zinc
Finger, TALE, and CRISPR plasmids over time.
• We’ll analyze CRISPR plasmids in more detail by
summarizing the latest plasmid developments and by
examining trends in genome engineering technologies.
Finally, we’ll present data describing the research
tools shared by synthetic biology labs.
3. Genome Engineering and Synthetic Biology
Plasmids Deposited with Addgene
The data reveal the ebb and flow of genome
engineering technology trends.
4. Genome Engineering and Syn Bio Plasmids in
the Collection
Addgene’s
collection includes
35,000 plasmids
from 2,100 labs
worldwide.
Genome
engineering and
synthetic biology
plasmids make up
approximately 10%
of the total
collection.
5. CRISPR Plasmid Requests Outpace Other
Technologies
The power and broad applicability of CRISPR technology
is evident as the number of requested CRISPR plasmids
outpaces other engineering technologies.
6. CRISPR Technologies at Addgene
Visualization of the major CRISPR technologies by function in Addgene’s
collection ordered from left to right by frequency of requests.
The CRISPR collection contains >550 plasmids contributed by 68 depositing labs.
7. Synthetic Biology Technologies at Addgene
Visualization of SynBio tools in Addgene’s collection grouped from left to right
by frequency of requests.
The entire Addgene SynBio collection includes > 1,250 plasmids contributed by
46 depositing labs.
8. Syn Bio Depositing Laboratories
Hal Alper
Christopher Anderson
Adam Arkin
Gabor Balazsi
Matthew Bennett
Carolyn Bertozzi
Nicolas Buchler
Cynthia Collins
James Collins
VÃctor de Lorenzo
Colin Dolphin
Tom Ellis
Drew Endy
Susan Golden
John Gray
Je Hasty
Joshua Heazlewood
Gabor Igloi
Farren Isaacs
Dieter Jahn
Alfonso Jaramillo
Roman Jerala
Jay Keasling
Brian Kuhlman
Taek Soon Lee
Joshua Leonard
Han Lim
Wendell Lim
Michela Lizier
Michel Maharbiz
Thorsten Mascher
Ichiro Matsumura
Anastasios Melis
Bradley Moore
Aindrila
Mukhopadhyay
Richard Murray
Erin OShea
Antonio Richart
Herbert Sauro
Claudia Schmidt-
Dannert
Julian Schroeder
Pamela Silver
Jerey Tabor
Christopher Voigt
Cliord Wang
Philip Weyman
9. CRISPR Depositing Laboratories
Mario de Bono
Mike Boxem
Simon Bullock
John Calarco
Wenbiao Chen
Qi-Jun Chen
Yonglong Chen
Lionel Christiaen
George Church
Jennifer Doudna
Peter Duchek
Benjamin Ebert
Andrew Fire
Klaus Foerstemann
Hodaka Fujii
Wataru Fujii
Caixia Gao
Charles Gersbach
Bob Goldstein
Manuel Goncalves
Izuho Hatada
Akitsu Hotta
Shenglin Huang
Xingxu Huang
Danwei Huangfu
Masahito Ikawa
Tyler Jacks
Rudolf Jaenisch
Renjie Jiao
Keith Joung
Sophien Kamoun
Iskra Katic
Hyongbum Kim
Jin-Soo Kim
Masato Kinoshita
David Liu
Ji-Long Liu
Sebastian Lourido
Timothy Lu
Luciano Marrani
Craig Mello
Kiran Musunuru
Kate O'Connor-
Giles
Wayne Parrott
Stanley Qi
David Root
David Sabatini
Alex Schier
Jen Sheen
David Sibley
William Simonds
Paul Sternberg
Robert Stupar
James Thomson
Ron Vale
Wensheng Wei
Jonathan
Weissman
Susan Wente
Jill Wildonger
Scot Wolfe
Takashi Yamamoto
Yinong Yang
Kosuke Yusa
Donald Zack
Mikel Zaratiegui
Feng Zhang
Huimin Zhao
Yunde Zhao
10. Addgene Statistics
>35,000 plasmids stored
>470,000 plasmids shipped to >5,000 institutions in 78 countries
From >2,100 contributing labs, >500 institutions worldwide
>2,000 plasmids shipped each week
51% shipped to scientists outside the United States
“I do science differently because
I can use the Addgene library to
find reagents”
11. Benefits of a Central Plasmid Repository
Save time on request mailing
Access all plasmids in one request
No loss due to turnover of lab members
New labs hear about your work, more citations
Archive historical clones and standards, rigorous QC
Get a list of who has your plasmids any time (for grants or
Tech Transfer offices)
Collaborate! Participate in our Community!
12. Learn More About Addgene
Addgene accelerates research and discovery by
helping researchers easily share information,
access useful research materials, and increase
collaborations. Contributing plasmids to
Addgene’s collection is free and easy.
To learn more about the benefits of depositing to
and requesting from Addgene’s collection, visit
www.addgene.org