The valorization of the medicinal plants of our country and determination of their impact on health due to their abundance of substances with various pharmacological effects are our principal objective. This study was evaluated the phytochemical screening and radical 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of different extracts of Sakersia africana Hook. f.. The results revealed that Sakersia africana Hook. f. is rich in phenols compounds, sterols, triterpenes, alkaloids and reducing compound. The values in total phenols and proanthocyanidines are ranging respectively from 391.58 ± 0.04 to 777 ± 0.03 mg/100 g of drugs and 113.5 ± 3.17 to 653.5 ± 36.83 mg/100 g of drugs. Results also show that different extracts tested present antiradical activity with values of IC50 ranging from 164.21± 0.014 to 195.54± 0.012 % and abundance in bioactive compounds. This study could justify the use of Sakersia africana of some chronic diseases.
GC-MS Analysis and Evaluation of In-vitro Antioxidant Potential and Total Phe...Innspub Net
Wild hops (Flemingia strobilifera Linn.) is a shrub belonging to Fabaceae family. The leaves of F. strobilifera are commonly used by the Subanen, the indigenous group in Lapuyan, Zamboanga del Sur, Philippines to treat inflammation. In this study, the hydromethanolic (80%) and aqueous leaf extracts of F. strobilifera were evaluated for their antioxidant activity and total phenolics content. The active semi-volatile components of 80% methanol leaf extract were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). DPPH radical scavenging activity was used to determine the potential of this plant as anti-oxidant. Total phenolics content was determined using Folin-Ciocalteu reagent and calculated as gallic acid equivalence. GC-MS analysis revealed the presence of eight compounds. Both the 80% methanol and aqueous extracts of F. strobilifera showed significant scavenging activity with low IC50 values of 0.299 mg/mL and <0.25 mg/mL, respectively. There was positive correlation between the scavenging activity percentage and the total phenolics content only in the aqueous extract of F. strobilifera while 80% methanol extract showed negative correlation between inhibition percentage and total phenolics content which can be attributed to the solvent used and method used in quantification of phenolics. Nevertheless, the results suggest that these leaf extracts are potent source of antioxidant compounds and may serve as natural anti-inflammatory agents.
GC-MS Analysis and Evaluation of In-vitro Antioxidant Potential and Total Phe...Innspub Net
Wild hops (Flemingia strobilifera Linn.) is a shrub belonging to Fabaceae family. The leaves of F. strobilifera are commonly used by the Subanen, the indigenous group in Lapuyan, Zamboanga del Sur, Philippines to treat inflammation. In this study, the hydromethanolic (80%) and aqueous leaf extracts of F. strobilifera were evaluated for their antioxidant activity and total phenolics content. The active semi-volatile components of 80% methanol leaf extract were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). DPPH radical scavenging activity was used to determine the potential of this plant as anti-oxidant. Total phenolics content was determined using Folin-Ciocalteu reagent and calculated as gallic acid equivalence. GC-MS analysis revealed the presence of eight compounds. Both the 80% methanol and aqueous extracts of F. strobilifera showed significant scavenging activity with low IC50 values of 0.299 mg/mL and <0.25 mg/mL, respectively. There was positive correlation between the scavenging activity percentage and the total phenolics content only in the aqueous extract of F. strobilifera while 80% methanol extract showed negative correlation between inhibition percentage and total phenolics content which can be attributed to the solvent used and method used in quantification of phenolics. Nevertheless, the results suggest that these leaf extracts are potent source of antioxidant compounds and may serve as natural anti-inflammatory agents. Get the full articles at: http://www.innspub.net/ijb/gc-ms-analysis-and-evaluation-of-in-vitro-antioxidant-potential-and-total-phenolics-content-of-wild-hops-flemingia-strobilifera-l-w-t-aiton/
Antioxidant activity against methanol extraction of eucheuma cotonii and e. s...Alexander Decker
The document summarizes a study that tested the antioxidant activity of Eucheuma cotonii and E. spinosum collected from North Sulawesi, Indonesia. Fresh and dry samples of both algae were extracted using 60%, 70%, and 80% methanol solvent. Tests measured total phenol content, DPPH radical scavenging activity, FRAP antioxidant power, and total carotene. The highest values were found in fresh E. spinosum extracted with 60% methanol, indicating it had the strongest antioxidant activity among the samples tested.
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EX...bioejjournal
This study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant
activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet
- mansourah at Tlemcen city in the West Northern of Algeria.
In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary
metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 μg/ml against several strains of bacteria and MIC=45.16 μg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed
good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220μg/mL for
hydroethanol extract.
Antioxidant and Antimicrobial Activities Of Algerian Populus Nigra L. Buds Ex...bioejjournal
This study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet - mansourah at Tlemcen city in the West Northern of Algeria. In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for hydroethanol extract.
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EX...bioejjournal
his study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet - mansourah at Tlemcen city in the West Northern of Algeria. In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC= 90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for hydroethanol extract.
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EX...bioejjournal
In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary
metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed
good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for
hydroethanol extract.
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EX...bioejjournal
This study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant
activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet
- mansourah at Tlemcen city in the West Northern of Algeria.
In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary
metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed
good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for
hydroethanol extract.
GC-MS Analysis and Evaluation of In-vitro Antioxidant Potential and Total Phe...Innspub Net
Wild hops (Flemingia strobilifera Linn.) is a shrub belonging to Fabaceae family. The leaves of F. strobilifera are commonly used by the Subanen, the indigenous group in Lapuyan, Zamboanga del Sur, Philippines to treat inflammation. In this study, the hydromethanolic (80%) and aqueous leaf extracts of F. strobilifera were evaluated for their antioxidant activity and total phenolics content. The active semi-volatile components of 80% methanol leaf extract were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). DPPH radical scavenging activity was used to determine the potential of this plant as anti-oxidant. Total phenolics content was determined using Folin-Ciocalteu reagent and calculated as gallic acid equivalence. GC-MS analysis revealed the presence of eight compounds. Both the 80% methanol and aqueous extracts of F. strobilifera showed significant scavenging activity with low IC50 values of 0.299 mg/mL and <0.25 mg/mL, respectively. There was positive correlation between the scavenging activity percentage and the total phenolics content only in the aqueous extract of F. strobilifera while 80% methanol extract showed negative correlation between inhibition percentage and total phenolics content which can be attributed to the solvent used and method used in quantification of phenolics. Nevertheless, the results suggest that these leaf extracts are potent source of antioxidant compounds and may serve as natural anti-inflammatory agents.
GC-MS Analysis and Evaluation of In-vitro Antioxidant Potential and Total Phe...Innspub Net
Wild hops (Flemingia strobilifera Linn.) is a shrub belonging to Fabaceae family. The leaves of F. strobilifera are commonly used by the Subanen, the indigenous group in Lapuyan, Zamboanga del Sur, Philippines to treat inflammation. In this study, the hydromethanolic (80%) and aqueous leaf extracts of F. strobilifera were evaluated for their antioxidant activity and total phenolics content. The active semi-volatile components of 80% methanol leaf extract were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). DPPH radical scavenging activity was used to determine the potential of this plant as anti-oxidant. Total phenolics content was determined using Folin-Ciocalteu reagent and calculated as gallic acid equivalence. GC-MS analysis revealed the presence of eight compounds. Both the 80% methanol and aqueous extracts of F. strobilifera showed significant scavenging activity with low IC50 values of 0.299 mg/mL and <0.25 mg/mL, respectively. There was positive correlation between the scavenging activity percentage and the total phenolics content only in the aqueous extract of F. strobilifera while 80% methanol extract showed negative correlation between inhibition percentage and total phenolics content which can be attributed to the solvent used and method used in quantification of phenolics. Nevertheless, the results suggest that these leaf extracts are potent source of antioxidant compounds and may serve as natural anti-inflammatory agents. Get the full articles at: http://www.innspub.net/ijb/gc-ms-analysis-and-evaluation-of-in-vitro-antioxidant-potential-and-total-phenolics-content-of-wild-hops-flemingia-strobilifera-l-w-t-aiton/
Antioxidant activity against methanol extraction of eucheuma cotonii and e. s...Alexander Decker
The document summarizes a study that tested the antioxidant activity of Eucheuma cotonii and E. spinosum collected from North Sulawesi, Indonesia. Fresh and dry samples of both algae were extracted using 60%, 70%, and 80% methanol solvent. Tests measured total phenol content, DPPH radical scavenging activity, FRAP antioxidant power, and total carotene. The highest values were found in fresh E. spinosum extracted with 60% methanol, indicating it had the strongest antioxidant activity among the samples tested.
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EX...bioejjournal
This study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant
activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet
- mansourah at Tlemcen city in the West Northern of Algeria.
In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary
metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 μg/ml against several strains of bacteria and MIC=45.16 μg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed
good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220μg/mL for
hydroethanol extract.
Antioxidant and Antimicrobial Activities Of Algerian Populus Nigra L. Buds Ex...bioejjournal
This study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet - mansourah at Tlemcen city in the West Northern of Algeria. In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for hydroethanol extract.
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EX...bioejjournal
his study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet - mansourah at Tlemcen city in the West Northern of Algeria. In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC= 90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for hydroethanol extract.
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EX...bioejjournal
In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary
metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed
good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for
hydroethanol extract.
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EX...bioejjournal
This study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant
activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet
- mansourah at Tlemcen city in the West Northern of Algeria.
In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary
metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed
good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for
hydroethanol extract.
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EX...bioejjournal
This study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant
activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet
- mansourah at Tlemcen city in the West Northern of Algeria.
In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary
metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed
good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for
hydroethanol extract.
Antioxidant properties of phenolic extracts of african mistletoes (loranthus ...Alexander Decker
This document summarizes a study that investigated the antioxidant properties of phenolic extracts from the African mistletoe (Loranthus begwensis L.) harvested from kolanut and breadfruit trees. The study found that mistletoe from the kolanut tree had higher total phenol content and stronger antioxidant effects, including higher DPPH radical scavenging ability and ferric reducing ability, compared to mistletoe from the breadfruit tree. However, mistletoe from the breadfruit tree showed higher ability to chelate Fe2+ ions. Overall, both mistletoe extracts displayed promising antioxidant properties, but the capacity varied depending on the host tree.
Antioxidant properties of phenolic extracts of african mistletoes (loranthus ...Alexander Decker
1) The study investigated the antioxidant properties of phenolic extracts from the African mistletoe Loranthus begwensis L. harvested from kolanut and breadfruit trees.
2) The results showed that mistletoe from the kolanut tree had higher total phenol and ferric reducing content than mistletoe from the breadfruit tree.
3) Both mistletoe extracts demonstrated antioxidant effects, including scavenging of free radicals and chelating of iron ions. However, the antioxidant capacity was dependent on the host tree, with mistletoe from the kolanut tree generally showing higher antioxidant activity.
This study investigated the antioxidant, anticancer, and chemopreventive properties of Moringa oleifera leaf extracts. Methanol and dichloromethane extracts were tested for their antioxidant activity using DPPH and ABTS radical scavenging assays. The extracts were also evaluated for their antiproliferative effects on liver, colon, and breast cancer cell lines, as well as their ability to induce quinone reductase activity in liver cells as a measure of cancer chemoprevention. The results showed that the methanol extract had higher radical scavenging activity than the dichloromethane extract. Both extracts inhibited the growth of cancer cells, but the dichloromethane extract more strongly induced
The word “banana” is a general term comprising a number of species or hybrids in the genus Musa of family Musaceae. Many studies reported that banana is a good source of phenolic compounds, which are having potential health benefits in human beings. The phytochemical composition of eight banana varieties was investigated. Quantitative estimation of phytochemicals revealed that highest alkaloid and tannin content was noticed in variety Nendran (3.76% and 4.40 mg/100g respectively). Whereas, variety Rasakadali exhibited highest flavonoid content (9.49 mg/100). Total phenol and saponin content was found higher in variety Kadali (0.82% and 11.6 mg/100g respectively).
Antioxidant and-anticancer-activities-of-moringa-leavesSilentdisco Berlin
Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
Phytochemical analyses of aqueous extracts of two medicinal plants from gabon...Alexander Decker
This article summarizes a study that analyzed the phytochemical composition of aqueous extracts of two medicinal plants used in Gabon, Pseudospondias longifolia and Antrocaryon klaineanum. Phytochemical screening found alkaloids, saponins, tannins, and reductor compounds in extracts of both plants, while flavonoids were only present in P. longifolia extracts. Sterols and terpenoids were not detected in either plant. The results provide insight into the biochemical basis for the traditional medicinal uses of the plants.
Influence of phosphorous acid application on the accumulation of total phenol...Innspub Net
One mechanism used by coconut plant to protect itself against Phytophthorakatsurae is linked to total
polyphenols production. This study aimed to investigate the impact of phosphorous acid plant treatment on the
production of total polyphenols in coconuthusk, as part of chemical control.The study was conducted on two
coconuts cultivars (EGD and PB 121+) with four doses of phosphorous acid [Control, 2.8 g (TA), 5.6 g (TB), 11.2 g
(TC)]. At each sampling, the husks were processed and extracts were prepared for total polyphenols assays. There
was significant difference between EGD and PB 121+ total polyphenols production (p<0.001).The interaction
between coconut variety and phosphorous acid doses was also significant.The interaction EGD and TC had the
highest total polyphenols accumulation of 4838.5 µg/g of fresh weight (FW). For PB121+, the highest total polyphenols accumulation of 6433.71 µg/g FW was obtained from the interaction between PB121+ and T0. From this observation, it could be statedthat phosphorous acid only triggers the treated plantdefense mechanisms to produce total phenolic compounds when attacked by a pathogen. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
Phytochemical Analysis of some Macrophytes of Lake Kondakarla, Visakhapatnam ...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Pharmacognostic, antioxidant and antimicrobial activity of fractions of the l...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
This document summarizes a study that investigated the pharmacognostic, antioxidant, and antimicrobial properties of Kigelia africana Lam. Benth leaves. Pharmacognostic evaluation of the leaf's physical characteristics and microscopic anatomy was performed. Phytochemical analysis revealed the presence of alkaloids, flavonoids, tannins, and saponins. The crude methanol extract and fractions showed significant antioxidant activity in DPPH radical scavenging assays. Testing against bacterial and fungal strains found that the crude extract and fractions inhibited the growth of most pathogens, except the aqueous fraction. The results support traditional use of K. africana for treating infectious diseases.
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Phenolic compounds from artichoke (cynara scolymus l.) by Alexander Decker
The document discusses phenolic compounds extracted from artichoke (Cynara scolymus L.) byproducts and their antimicrobial activities. It finds that methanolic extracts of bound phenols from artichoke bracts and hearts showed the highest antimicrobial activity against gram-negative bacteria. The minimum inhibitory concentration values for bound phenols from hearts were 63μg/ml. Free phenols from hearts also demonstrated antimicrobial properties, with minimum inhibitory concentration values ranging from 204 to 206 μg/mL against both gram-positive and gram-negative microorganisms. The study suggests artichoke byproducts could be used to develop nutritional supplements with antimicrobial properties.
Phenolic compounds from artichoke (cynara scolymus l.) by Alexander Decker
This document summarizes a study that evaluated the antimicrobial activity of phenolic compounds extracted from different parts of artichoke plants. The researchers found that methanolic extracts of bound phenols from artichoke bracts and hearts showed the highest antimicrobial activity against gram-negative bacteria. Specifically, bound phenols from hearts had MIC values of 63μg/ml, while bound phenols from bracts ranged from 312-486μg/ml against gram-negative bacteria and 486μg/ml against gram-positive bacteria. Free phenols from hearts also demonstrated strong antimicrobial activity with MIC values of 204-206μg/ml against both gram-positive and gram-negative bacteria. Therefore, the
Effects of organic and mineral fertilizers on total antioxidant, polyphenolic...Alexander Decker
This study examined the effects of organic and mineral fertilizers on antioxidant, polyphenol, and carotenoid content in orange-fleshed sweet potato tubers. Organic fertilizer significantly increased all three phytochemicals, with annual applications having the highest levels. Mineral fertilizers also significantly affected levels, with combinations of nitrogen, phosphorus, and potassium influencing different compounds. The combination of minimal mineral doses with annual or biennial organic fertilizer produced the highest antioxidant, polyphenol, and carotenoid concentrations in the tubers.
This study analyzed 31 Thai medicinal plant species commonly used in traditional medicine in northeast Thailand. The plants were grouped into digestive tonic, diarrheal relief, anti-tussive, and anti-inflammation categories based on their traditional uses. The study determined total antioxidant activity, free radical scavenging activity, total phenolic content, ascorbic acid content, and levels of some phytochemicals for the plants. The results showed variation in these biochemical parameters among the different plant groups. Anti-tussive plants generally had higher total antioxidant activity and phenolic/ascorbic acid levels than diarrheal relief and anti-inflammation plants. Digestive tonic plants exhibited strong free radical scavenging activity.
Influence of gongronema latifolium leaf extracts treatment on some hepatic...Alexander Decker
This document summarizes a study on the effects of extracts from the leaves of Gongronema latifolium on hepatic enzyme activity in rats. Rats were treated with various doses of ethanolic and water extracts for 7 days. Blood samples were then analyzed for liver enzyme levels. The results showed that the extracts increased the activity of AST and ALT liver enzymes at doses of 25mg/kg, indicating potential liver cell damage. The extracts also increased serum amylase levels in a dose-dependent manner. However, the extracts did not significantly affect blood urea levels. The conclusion is that while G. latifolium extracts may have some medical benefits, chronic high dose use could adversely impact liver function.
Antioxidant and antiproliferative effects on human liver hepg2epithelial cell...Alexander Decker
This document summarizes a study that evaluated the antioxidant and antiproliferative effects of artichoke (Cynara scolymus L.) and its byproducts on human liver (HePG2) epithelial cells. The study found that the free phenolic extract of artichoke bracts showed significant antioxidant activity in DPPH radical scavenging and iron chelating assays. This extract also exhibited the highest antiproliferative effects against HePG2 cells, reducing viability by 78.3%, higher than the heart extract's effect of 36.7%. Therefore, artichoke byproducts contain compounds with antioxidant and anticancer properties and may provide nutritional supplements.
Water sources and management practices among the household residents of Baran...Open Access Research Paper
The purpose of this study is to determine the water sources and management practices among the household residents of Barangay Labuyo, Tangub City, Misamis Occidental. A total of 204 household respondents were randomly interviewed using a survey questionnaire. Data on water management practices were measured using a 5-point Likert scale. One-way ANOVA and Pearson correlation were further used to determine the differences and relationships between demographic profiles and management practices. Results show that pipe water supply from the main source (68%) was the primary water source used among household residents. In terms of sex, females often practice water management on the water sources with a weighted mean of 3.42. While respondents with age 68 years and older exhibit a higher degree (weighted mean=3.63) of involvement in water conservation measures. And respondents with college degree have applied their in-depth comprehension and knowledge on water conservation with a weighted mean of 3.48. The study also revealed a significant difference in the management practices between two sexes, among all ages and levels of educational attainment with p values <0.05. The association between demographic profiles with management practices further presents a significant relationship. Generally, the demographic profile (sex, age, and educational attainment) has a weak positive relationship towards management practices with values, r = 0.26, p = 0.00021; r = 0.34, p = <0.05; and r = 0.26, p = 0.00014, respectively. Thus, results suggest the need for enhancing community awareness on sustainable water source management and putting it into practice consequently reducing inadequacy of water supply in the area.
The modification of an existing product or the formulation of a new product to fill a newly identified market niche or customer need are both examples of product development. This study generally developed and conducted the formulation of aramang baked products enriched with malunggay conducted by the researchers. Specifically, it answered the acceptability level in terms of taste, texture, flavor, odor, and color also the overall acceptability of enriched aramang baked products. The study used the frequency distribution for evaluators to determine the acceptability of enriched aramang baked products enriched with malunggay. As per sensory evaluation conducted by the researchers, it was proven that aramang baked products enriched with malunggay was acceptable in terms of Odor, Taste, Flavor, Color, and Texture. Based on the results of sensory evaluation of enriched aramang baked products proven that three (3) treatments were all highly acceptable in terms of variable Odor, Taste, Flavor, Color and Textures conducted by the researchers.
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Antioxidant properties of phenolic extracts of african mistletoes (loranthus ...Alexander Decker
This document summarizes a study that investigated the antioxidant properties of phenolic extracts from the African mistletoe (Loranthus begwensis L.) harvested from kolanut and breadfruit trees. The study found that mistletoe from the kolanut tree had higher total phenol content and stronger antioxidant effects, including higher DPPH radical scavenging ability and ferric reducing ability, compared to mistletoe from the breadfruit tree. However, mistletoe from the breadfruit tree showed higher ability to chelate Fe2+ ions. Overall, both mistletoe extracts displayed promising antioxidant properties, but the capacity varied depending on the host tree.
Antioxidant properties of phenolic extracts of african mistletoes (loranthus ...Alexander Decker
1) The study investigated the antioxidant properties of phenolic extracts from the African mistletoe Loranthus begwensis L. harvested from kolanut and breadfruit trees.
2) The results showed that mistletoe from the kolanut tree had higher total phenol and ferric reducing content than mistletoe from the breadfruit tree.
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IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
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The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
This document summarizes a study that investigated the pharmacognostic, antioxidant, and antimicrobial properties of Kigelia africana Lam. Benth leaves. Pharmacognostic evaluation of the leaf's physical characteristics and microscopic anatomy was performed. Phytochemical analysis revealed the presence of alkaloids, flavonoids, tannins, and saponins. The crude methanol extract and fractions showed significant antioxidant activity in DPPH radical scavenging assays. Testing against bacterial and fungal strains found that the crude extract and fractions inhibited the growth of most pathogens, except the aqueous fraction. The results support traditional use of K. africana for treating infectious diseases.
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Phenolic compounds from artichoke (cynara scolymus l.) by Alexander Decker
The document discusses phenolic compounds extracted from artichoke (Cynara scolymus L.) byproducts and their antimicrobial activities. It finds that methanolic extracts of bound phenols from artichoke bracts and hearts showed the highest antimicrobial activity against gram-negative bacteria. The minimum inhibitory concentration values for bound phenols from hearts were 63μg/ml. Free phenols from hearts also demonstrated antimicrobial properties, with minimum inhibitory concentration values ranging from 204 to 206 μg/mL against both gram-positive and gram-negative microorganisms. The study suggests artichoke byproducts could be used to develop nutritional supplements with antimicrobial properties.
Phenolic compounds from artichoke (cynara scolymus l.) by Alexander Decker
This document summarizes a study that evaluated the antimicrobial activity of phenolic compounds extracted from different parts of artichoke plants. The researchers found that methanolic extracts of bound phenols from artichoke bracts and hearts showed the highest antimicrobial activity against gram-negative bacteria. Specifically, bound phenols from hearts had MIC values of 63μg/ml, while bound phenols from bracts ranged from 312-486μg/ml against gram-negative bacteria and 486μg/ml against gram-positive bacteria. Free phenols from hearts also demonstrated strong antimicrobial activity with MIC values of 204-206μg/ml against both gram-positive and gram-negative bacteria. Therefore, the
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This study examined the effects of organic and mineral fertilizers on antioxidant, polyphenol, and carotenoid content in orange-fleshed sweet potato tubers. Organic fertilizer significantly increased all three phytochemicals, with annual applications having the highest levels. Mineral fertilizers also significantly affected levels, with combinations of nitrogen, phosphorus, and potassium influencing different compounds. The combination of minimal mineral doses with annual or biennial organic fertilizer produced the highest antioxidant, polyphenol, and carotenoid concentrations in the tubers.
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Influence of gongronema latifolium leaf extracts treatment on some hepatic...Alexander Decker
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Antioxidant and antiproliferative effects on human liver hepg2epithelial cell...Alexander Decker
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Water sources and management practices among the household residents of Baran...Open Access Research Paper
The purpose of this study is to determine the water sources and management practices among the household residents of Barangay Labuyo, Tangub City, Misamis Occidental. A total of 204 household respondents were randomly interviewed using a survey questionnaire. Data on water management practices were measured using a 5-point Likert scale. One-way ANOVA and Pearson correlation were further used to determine the differences and relationships between demographic profiles and management practices. Results show that pipe water supply from the main source (68%) was the primary water source used among household residents. In terms of sex, females often practice water management on the water sources with a weighted mean of 3.42. While respondents with age 68 years and older exhibit a higher degree (weighted mean=3.63) of involvement in water conservation measures. And respondents with college degree have applied their in-depth comprehension and knowledge on water conservation with a weighted mean of 3.48. The study also revealed a significant difference in the management practices between two sexes, among all ages and levels of educational attainment with p values <0.05. The association between demographic profiles with management practices further presents a significant relationship. Generally, the demographic profile (sex, age, and educational attainment) has a weak positive relationship towards management practices with values, r = 0.26, p = 0.00021; r = 0.34, p = <0.05; and r = 0.26, p = 0.00014, respectively. Thus, results suggest the need for enhancing community awareness on sustainable water source management and putting it into practice consequently reducing inadequacy of water supply in the area.
The modification of an existing product or the formulation of a new product to fill a newly identified market niche or customer need are both examples of product development. This study generally developed and conducted the formulation of aramang baked products enriched with malunggay conducted by the researchers. Specifically, it answered the acceptability level in terms of taste, texture, flavor, odor, and color also the overall acceptability of enriched aramang baked products. The study used the frequency distribution for evaluators to determine the acceptability of enriched aramang baked products enriched with malunggay. As per sensory evaluation conducted by the researchers, it was proven that aramang baked products enriched with malunggay was acceptable in terms of Odor, Taste, Flavor, Color, and Texture. Based on the results of sensory evaluation of enriched aramang baked products proven that three (3) treatments were all highly acceptable in terms of variable Odor, Taste, Flavor, Color and Textures conducted by the researchers.
Microbiological assessment of air quality of selected locations within Verita...Open Access Research Paper
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Water polluted by dyestuffs compounds is a global threat to health and the environment; accordingly, we prepared a green novel sorbent chemical and Physical system from an algae, chitosan and chitosan nanoparticle and impregnated with algae with chitosan nanocomposite for the sorption of Malachite green dye from water. The algae with chitosan nanocomposite by a simple method and used as a recyclable and effective adsorbent for the removal of malachite green dye from aqueous solutions. Algae, chitosan, chitosan nanoparticle and algae with chitosan nanocomposite were characterized using different physicochemical methods. The functional groups and chemical compounds found in algae, chitosan, chitosan algae, chitosan nanoparticle, and chitosan nanoparticle with algae were identified using FTIR, SEM, and TGADTA/DTG techniques. The optimal adsorption conditions, different dosages, pH and Temperature the amount of algae with chitosan nanocomposite were determined. At optimized conditions and the batch equilibrium studies more than 99% of the dye was removed. The adsorption process data matched well kinetics showed that the reaction order for dye varied with pseudo-first order and pseudo-second order. Furthermore, the maximum adsorption capacity of the algae with chitosan nanocomposite toward malachite green dye reached as high as 15.5mg/g, respectively. Finally, multiple times reusing of algae with chitosan nanocomposite and removing dye from a real wastewater has made it a promising and attractive option for further practical applications.
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The evolving uncertainty of the emergence of the COVID-19 pandemic has adversely impacted some commodity prices, the welfare of farming households, and the economic growth and development of the country. The study is aimed at assessing the effects of Covid -19 pandemic on commodity price volatility and the welfare of farming households in Nigeria. The study was based on secondary data from (2010- 2022, and forecasts from 2023 -2026), collected from the Central Bank of Nigeria publication, IMF World economic outlook, and the World Bank report. Time- response graph and Autoregressive Moving Average (ARMA) was used to analyze the trend of the inflation rate, and T-test statistics, were used to test the relationship between the real income of farming household before and during the pandemic. The result showed that prices of some commodities doubled after the pandemic. There is a significant difference between the real income of farming households before and during the pandemic. The study recommends that a price control mechanism should be put in place to manage the affordability of goods and services in the market because this has a direct effect on the growth and development of the country.
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The tomato is the one of the most famous crops in Pakistan. It is used and consumed as fresh as well as in processed form. Its botanical name is Solanum lycopersicon Mill. The proposed study had been conducted in the year 2017-2018 in order to find the best combination of water soluble NPK fertilizers as compared to control. Five different types of water soluble fertilizers were collected from different sources and were applied at 5% concentration during the entire growing period of the tomato crop. The experiment was designed using Randomized Complete Block Design (RCBD) with five treatments and three replications of each. Different vegetative, reproductive and bio-chemical parameters were recorded and analyzed statistically at 5% level of significance. The treatments were compared using LSD test. It was concluded that different NPK water soluble fertilizers showed variation in physical and bio chemical parameters in tomato plants as compared to control. The plants showed variation in plant height, No. of fruit per plant, fruit yield per hectare, Individual fruit weight, fruit weight per plant, No. of diseased fruit per plant, date of first harvest, Fruit color Fruit length (cm), fruit size (cm), fruit firmness, total NPK contents, vitamincmg Chlorophyll concentration, pH, (TSS), Electricity conductivity (EC) and Titratable acidity Among all the water soluble treatments, the T3 treatment (WSF 20:20:20) yielded the comparatively better results as compared to other WSF treatments. So it was concluded that T3 was the recommended water soluble fertilizer for tomato.
Yield response of aman rice to transplanting geometry and seedlings per hill ...Open Access Research Paper
In a condition of limited scope of horizontal yield expansion, rice yield can be increased by efficient utilization of land through proper transplanting arrangement with maximum number of plant population unit-1 area. Thus, the present experiment was conducted in the farmer’s field at Batiaghata upazila of Khulna district, Bangladesh during T. aman season (Jul-Nov) to evaluate the effect of transplanting geometry and number of seedlings hill-1 on growth and yield of aman rice (Binadhan-7, a short duration variety of its’ early harvest can create opportunity to cultivate winter crops in this region). The experiment had four types of transplanting geometry (single row rectangular system, single row triangular system, double row rectangular system and double row triangular system) and three levels of seedling hill-1 (3, 4 and 5 seedlings) with three replications. The results showed that individually transplanting geometry or number of seedlings hill-1 had substantial influence on yield attributes and yield but their interaction effect had non-significant influence on almost all measured parameters except number of tillers and effective tillers hill-1. The maximum grain yield (5.6 t ha-1) was achieved from double row triangular system yet the highest plant height, effective tillers hill-1, grain panicle-1, 1000 grain weight and straw yield were obtained from single row triangular system. In case of seeding hill-1, 3 seedlings hill-1 produced the highest grain yield (5.33 t ha-1) plant height, effective tillers hill-1, grain panicle-1, 1000 grain weight and straw yield. It can be concluded that double row transplanting geometry with 3 seedlings hill-1 can utilize the land efficiently for grain yield and be recommended for cultivation of Binadhan-7 in the coastal region of south-western Bangladesh.
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Water contamination is one of the major causes of water borne diseases worldwide. In Kenya, approximately 43% of people lack access to potable water due to human contamination. River Kuywa water is currently experiencing contamination due to human activities. Its water is widely used for domestic, agricultural, industrial and recreational purposes. This study aimed at characterizing bacteria and fungi in river Kuywa water. Water samples were randomly collected from four sites of the river: site A (Matisi), site B (Ngwelo), site C (Nzoia water pump) and site D (Chalicha), during the dry season (January-March 2018) and wet season (April-July 2018) and were transported to Maseno University Microbiology and plant pathology laboratory for analysis. The characterization and identification of bacteria and fungi were carried out using standard microbiological techniques. Nine bacterial genera and three fungi were identified from Kuywa river water. Clostridium spp., Staphylococcus spp., Enterobacter spp., Streptococcus spp., E. coli, Klebsiella spp., Shigella spp., Proteus spp. and Salmonella spp. Fungi were Fusarium oxysporum, Aspergillus flavus complex and Penicillium species. Wet season recorded highest bacterial and fungal counts (6.61-7.66 and 3.83-6.75cfu/ml) respectively. The results indicated that the river Kuywa water is polluted and therefore unsafe for human consumption before treatment. It is therefore recommended that the communities to ensure that they boil water especially for drinking.
High histological grade breast cancer morphological evaluation on mammogram u...Open Access Research Paper
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Mortality rate of children under the age of five has reduced worldwide, but still the probability of a child dying before the age of five is greatest in underdeveloped countries. Pakistan reports child mortality rates in same bracket as other South Asian countries due to malnutrition and diarrhea. To determine the prevalence and factors associated in children less than five years of age a cross sectional study was conducted with mothers whose children were admitted in pediatric government Hospital, Lahore. A convenient sample of 101 children (6-59 months, 53 males and 48 females) suffering from malnutrition and diarrhea were selected from hospital. Data about socio demographic, anthropometric, clinical and dietary variables were collected and analyzed by using SPSS version 16. The results showed that mean age of patients was 19.36 ± 10.5 months. The illiteracy rate among mother and father of patients was 94.1% and 69.3% respectively. 94.1% of the patients were breastfed while 47.5% of the patients were on bottle feed with breast milk. 66.3% patients families has very low-income rate while 33.7% were satisfactory. 40.6% patients were not vaccinated against immunization. Out of 101 patients, 39.6% of the patients had diarrhea while 6.9% of the patients had chronic diarrhea. The prevalence rate of diarrhea (39%) was less among children who were younger than 18 months as compared to those who were above 18 months (40.5%). The most significant factors that caused the incidence of diarrhea in children was form of water storage system, complementary feeding practices, and hand wash cleaning materials. This study concluded that government, nongovernmental organizations and families living with children and mothers could cooperate on strategies to minimize the risks of the diarrhea among children less than five years of age.
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Phytochemical composition and antiradical activity of Sakersia africana Hook. f. medicinal plant from Gabon
1. Akoué et al.
1 Int. J. Biomol. Biomed.
RESEARCH PAPER OPEN ACCESS
Phytochemical composition and antiradical activity of Sakersia africana
Hook. f. medicinal plant from Gabon
Gontran Nsi Akoué1,2
, Louis-Clément Obame2*
, Joseph Privat Ondo2
, Ibrahim Brama1
,
Elvis Simplice Otogo N’Nang2
, Skitt Yvan Tapoyo2
, Alain Souza1
1
Laboratoire de Physiologie Animale: Electrophysiologie-Pharmacologie-URAB, Université des Sciences
et Techniques de Masuku, BP 913, Franceville Gabon
2
Laboratoire de Substances Naturelles et de Synthèses Organométalliques, URCHI-Université des
Sciences et Techniques de Masuku, BP 943, Franceville Gabon
Article Published: 17 May 2013
Key words: Sakersia africana Hook.f., phytochemical screening, total phenols,
proanthocyanidins, antiradical activity.
Abstract
The valorization of the medicinal plants of our country and determination of their impact on health due to their
abundance of substances with various pharmacological effects are our principal objective. This study was evaluated
the phytochemical screening and radical 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of different
extracts of Sakersia africana Hook. f.. The results revealed that Sakersia africana Hook. f. is rich in phenols
compounds, sterols, triterpenes, alkaloids and reducing compound. The values in total phenols and
proanthocyanidines are ranging respectively from 391.58 ± 0.04 to 777 ± 0.03 mg/100 g of drugs and 113.5 ± 3.17 to
653.5 ± 36.83 mg/100 g of drugs. Results also show that different extracts tested present antiradical activity with
values of IC50 ranging from 164.21± 0.014 to 195.54± 0.012 % and abundance in bioactive compounds. This study
could justify the use of Sakersia africana of some chronic diseases.
*Corresponding Author: Louis-Clément Obame obamengonga@gmail.com, yacth58@hotmail.com
International Journal of Biomolecules and Biomedicine (IJBB)
ISSN: 2221-1063 (Print) 2222-503X (Online)
Vol. 3, No. 3, p. 1-8, 2013
http://www.innspub.net
2. Akoué et al.
2 Int. J. Biomol. Biomed.
Introduction
The plants were used for prehistory by Human for
nutritional and therapeutic requirement. Medicinal
plants are the major source of drugs through their rich
secondary metabolites (Fouché et al., 2000; Nostro et
al., 2000). Thus, medicinal plants are the main means
to fight against diseases by including populations of
underdeveloped countries. This practice was
abandoned in favor of modern medicine. We are
witnessing a return to use of medicinal plants over the
past decade. World Health Organization (WHO), in
2007 estimated that about 80 % of the population of
developing countries can be cured from plants
(Obame, 2009). In 2001, the studies have revealed that
approximately 25 % of prescriptions in the world for
drugs herbal and 60 to 70 % of antibacterial and
anticancer drugs are substances of natural origin (Rate,
2001). Hence the useful achieve the phytochemical
studies of medicinal plants for discovery of news
bioactive compounds. As such the WHO is currently
supporting clinical validation of some traditional
medicines. It is a shrub of the Melastomataceae family
that grows on moist soils and in secondary forest
(Walker and Sillans 1961). The leaves and twigs in a
hairy very steep, and the protruding ribs of a green-
red, turn yellow or red before falling. Fruit globular
form berries are very small and contain many tiny
seeds. The decoction of the leaves has healing
properties crushed. It is used as healing by gabonese
populations in southern and the treatment of several
diseases as dysentery, diarrhea, stomach ulcers and
stomach aches (Walker and Sillans, 1961). The
objective of this study was to evaluate the
phytochemical characteristics and radical scavenging
activity of different extracts of Sakersia africana Hook.
f.
Material and methods
Plant material
The branches of S. africana were collected in
september 2012 in Mbomo village, at 28 km of Oyem
city (Northern Province of Gabon). The plant was
toxonomically authenticated at the Laboratory of Plant
Biology and Ecology of University of Science and
Technology of Masuku (Franceville, Gabon), where a
voucher specimen was deposited.
Preparation of plant extract
The branches of Sakersia africana was air-dried at
room temperature for a total period six weeks and
pulverized to powder using a clean electric blender
(Model Phillips 190). A 25 g sample of the pulverized
branches of Sakersia africana was soaked in 500 mL
of solvent (ethanol, ethanol-water (50/50 v/v) and
water) and allowed to stand for 72 h with intermittent
stirring. This was filtered through a whatman No. 1
filter paper and the filtrate obtained was evaporated to
a dry mass using a rotary rotavaporator at 40 °C. The
residues recovered were dried in an oven at a
temperature of 65 °C. The extract obtained is stored in
vials protected from light until the completion of
various tests. The yields of the extracts (%) were
calculated (Boulenouar et al., 2009; Salem, 2009).
Phytochemical screening
Phytochemical tests the dry extracts (ethanolic,
ethanolic-water and aqueous) bioactive compounds
was realized using standard procedures. Two
extractions methods (maceration and decoction) were
used (Bruneton, 1999; Bidié et al., 2008). The extracts
were tested qualitatively for the presence of chemical
constituents such as sterols, terpenoids, tannins,
polyphenols, flavonoids, quinones and saponins (Paris
and Moyses, 1969; Bouquet and Debray 1971). The
alkaloids were tested by the method of Dragendorff
and Meyer (Bruneton, 1999).
Phenols and proanthocyanidins content extracts
The Folin-Ciocalteu method was used to measure total
amount of total phenols content (Singleton et al.,
1999). Aliquots of 0.25 mL of leaf extracts (1 mg/mL)
were mixed with 1.25 mL Folin–Ciocalteu reagent (0.2
N diluted in Methanol). A reagent blank using
methanol instead of sample was prepared. After 5 min
3. Akoué et al.
3 Int. J. Biomol. Biomed.
incubation at room temperature, 1 mL sodium
carbonate solution (7.5 %) was added. Samples were
incubated at room temperature for 1 h and the
absorbance was measured at 765 nm versus the
prepared blank. All tests were carried out in triplicate
and total phenols content was expressed as mg of gallic
acid equivalents (GAE) per 100 g of drug.
Table 1. Composition phytochemical extracts of Sakersia africana Hook.f.
Chemical compounds Aqueous Ethanolic-water Ethanolic
Saponins +++ + -
Gallic tannins ++ +++ +++
Catechic tannins +++ +++ +++
Anthraquinones - - -
Total phenols +++ +++ +++
Total flavonoids - +++ +++
Flavanonols - +++ -
Flavones - - +
Flavanones - - -
Coumarins +++ +++ ++
Digitoxin +++ +++ ++
Digitoxigenin - - -
Gitoxin - - -
Gitoxigenin - - -
Alkaloids +++ +++ +++
Sterols and triterpenes +++ +++ +++
Reducters compounds +++ +++ +++
+++ = High, ++ = Moderate; + = Low; -: negative test.
Table 2. Comparison of total phenolic compound, proanthocyanidins and antiradical activity of extracts de Sakersia
africana Hook.f.
Extracts
Yields (%) Total phenols
(mg GAE/100 g
of drug)
PAs (mg
APE/100 g of
drug)
Quota of PAs in
Total phenols (%)
DPPH:
IC50 (µg/ml)
Aqueous 4.04 507.42 ± 0.17 113.5 ± 3.17 22.37± 3.17 195.54± 0.012
Ethanolic-water 4.36 391.58 ± 0.04 341.83 ± 36.5 87.30 ± 36.5 164.21± 0.014
Ethanolic 5.56 777 ± 0.03 653.5 ± 36.83 84.11 ± 36.83 177.02± 0.013
Proanthocyanidins (PAs) were quantified with the
hydrolysis test of proanthocyanidins in a hot acid-
alcohol medium into anthocyanidins. This method
allows taking into account all the units of flavans-3-ols
constituting the polymers (Prigent, 2005). The heating
step destroys the anthocyanidins pigments generated
by flavan-4-ols and eliminates part of the chlorophyll
pigments. The routine assay is performed by mixing
0.16 mL (1 mg/mL) of the extract with 2.33 mL of 30 %
HCl-butanol solution (v/v). The mixture was put in
tightly closed tube and vortexed for 1 min.
Subsequently, the tube was heated at 100°C for 2 h and
after cooling, the absorbance was read at 550 nm.
Apple procyanidins (DP ≈ 7.4) treated as
4. Akoué et al.
4 Int. J. Biomol. Biomed.
aforementioned were used as a standard. Results were
expressed as apple procyanidins equivalent (APE).
Antiradical activity
DPPH spectrophotometric (quantitative) assay was
performed with some modifications (Brand-Williams
et al., 1995). Method was widely used to test the ability
of antioxidant bioactive compounds to activity as free
radical scavengers or hydrogen donors. This test is
based on the capacity of stable free radical 2, 2-
diphenyl-1-picrylhydrazyl to react with hydrogen (H)
donors, including phenols. It is used for the
quantification of antioxidants in the complex of
biological systems (Miliauskas et al., 2004). Each
sample of extract was prepared at different
concentrations (100, 200, 300 and 400 μg/mL). The
reaction mixture contained 1 mL of DPPH prepared at
a concentration 20 mg/L in methanol, and 1 ml of test
samples. After a 30 min reaction, the absorbance was
read at 517 nm and converted into percentage of
antiradical activity (AA %), using the following the
formula (Abdoul-Latif et al., 201O).
%DPPH radical scavenging = ((Abscontrol - Abssample))/
Abscontrol) X 100
Where,
Abscontrol is the absorbance of the blank;
Abssample is the absorbance of the sample.
Control contained 1 mL of DPPH solution and 3 mL of
methanol. The measurements of DPPH radical
scavenging activity were carried out for three sample
replications, and values are an average of three
replicates. IC50 is defined as the concentration of the
test sample leading to a 50 % inhibition of the DPPH
free radicals. IC50 value was calculated from the
separate linear regression of plots of the mean
percentage of the antioxidant activity against
concentration of the test compounds (mg /mL)
obtained from three replicate assays.
Results and discussion
Phytochemical screening
The results show that all extracts are rich in
sterols,triterpenes, tannins (catechic and gallic),
polyphenols, alkaloids, and reducing compounds.
Coumarins and cardiac glycosides (digitoxins) are
abundant in aqueous and ethanolic-water extracts.
Only the aqueous extract contains saponins and no
anthraquinons free flavanones, digitoxigenin, and
gitoxin gitoxigenine. Also, some extracts (ethanolic and
ethanolic-water) are rich in flavonoids total (Table 1).
Phytochemical analysis is very useful in the evaluation
of some active biological components of Sakersia
africana. The qualitative and quantitative analyses
were carried out in both dry extract show that Sakersia
africana is rich in phenolic compounds (polyphenols,
tannins, flavonoids) in terpenic and nitrogen
compounds. This wealth of pharmacological
compounds conferred to said plant several
pharmacological properties. The abundance of tannins
explains the use of Sakersia africana ethnotherapy for
the treatment of pathology identified during this
investigation. These are diseases such as hypertension,
dysentery, diarrhea, stomach ulcers and stomach
aches. Indeed, it has been shown that the higher the
plant is rich in tannic compounds will be more effective
for the treatment of diseases of hypertension,
dysentery, diarrhea, hemorrhoids, stomach ulcer,
stomach ache and some various venereal diseases
(Kerharo and Adjanohoun 1989; Pousset, 1989). In
addition, the healing properties of plants are correlated
with the abundance of tannins. This is the case for
example Lannea acida, rich in tannins, is used in
traditional medicine for wound healing (Sereme et al.,
2008). This could also justify the healing properties
attributed to Sakersia africana (Walker and Sillans
1961).
Total polyphenols, proanthocyanidins contents
The yields of different extracts of Sakersia africana
were respectively 4.04 % (aqueous), 4.36 % (ethanolic-
water) and 5.56 % (ethanolic) (table 2). The
concentrations of total phenols in the different extracts
of the plant of study are more significant in
5. Akoué et al.
5 Int. J. Biomol. Biomed.
comparison with those of the cereals (0,481 à 0,896
mg/g of matter) appreciably equal with those of other
plants such as Broccolis (11.7 mg /g of matter), the
gallic ones (9.9 mg/g of matter) and the fruits (23.1
mg/g of matter for the blackberry) (Vinson et al., 1995;
Wang and Lin, 2000).
Fig. 1. Antiradical activity extracts of Sakersia
africana Hook.f.
Fig. 2. Correlation between antioxidant activity and
total phenols, (R2 = 0.8605).
Levels of phenolic content were expressed in terms of
gallic acid equivalent (GAE). The equation of the right
and side of the proportioning of total phenolic content
by the method of Folin-Ciocalteu gave Y = 0.0012 X –
0.0004 with R2 = 0.9902 (Abdoul-Latif et al., 2012).
The HCl/butanol assay used here for the determination
of proanthocyanidins is more specific than many other
tests such as the vanillin assay (Makkar, 2000; Santos-
Buelga and Scalbert, 2000). The interferences, which
might result from flavan-4-ols conversion into
proanthocyanidins or from chlorophylls, may have
been minimized during the heating step (Prigent,
2005; Santos-Buelga and Scalbert, 2000).
Levels of proanthocyanidins were expressed in terms
of apple proanthocyanidins equivalent (APE). The
equation of the right-hand side of the proportioning of
the proanthocyanidins by the HCl- Butanol method
gave Y = 0.0006 X + 0.0024 with R2 = 0.9869
(Abdoul-Latif et al., 2012) Among extracts,
proanthocyanidins contents ranged between 113.5 and
653.5 mg APE/100 g of drug (Table 2).
The total contents of phenols ranged between 391.58
and 777 mg GAE/100 g of drug (table 2). However,
87.30 and 84.11 % of total phenols in ethanolic-water
extracts and ethanolic respectively are
proanthocyanidins. Only the aqueous extract has a low
percentage of proanthocyanidins (22.37 %).
Correlation between antioxidant activity and total
phenols
Several comprehensive works have been done on the
effects of phenolic compounds on total antioxidants (Li
et al., 2008; Kim et al., 2009; Kouamé et al., 2009 and
Abdoul-Latif et al., 2010), and correlations between
phenolic compounds and total antioxidants
(Chanwitheesuk et al., 2005; Li et al., 2008). This
same trend was also obtained in our study. There was a
good linear correlation (R2 = 0.8605, p < 0.05)
between the total phenolic content and the scavenging
of DPPH radical in each extract (Figure 1). These
results are similar to those obtained by Njintang and
collaborators in their study on antiradical activity and
polyphenol content of ethanolic extracts of Propolis
deed. These authors showed that the antiradical
activity was correlated with the polyphenol content in
different extracts (Njintang et al. 2012).
Antiradical activity
Antioxidant activity using DPPH radical-scavenging
assay expressed as IC50 value is showed in table 2 lower
IC50 indicating the higher antioxidant activity of
extract. The results of DPPH antiradical activity were
differed significantly between different extracts. This
difference is explained by the fact that an antiradical
activity of phenolic compounds depends on their
molecular structure, on the availability of phenolic
6. Akoué et al.
6 Int. J. Biomol. Biomed.
hydrogens and on the possibility for stabilization of the
resulting phenoxyl radicals formed by hydrogen
donation (Catherine et al., 1996; Ramarathnam et al.,
1997; Sundaram et al., 2011). These results of
antiradical activity of Sakersia africana show that
extracts exhibit antiradical activity from a
concentration of 50 μg/mL (aqueous and ethanolic-
water) (figure 1 and table 2). The radical scavenging
activity of ethanolic extract starts at concentrations
that are higher than 50 μg/mL. The lowest
concentration IC50 that inhibits half of the DPPH
radical was obtained with ethanolic-water extract
(164.21 mg/mL) followed by ethanol extract (177.02
mg/mL). The aqueous extract show the highest
inhibitory concentration (IC50 = 195.54 mg/mL) (table
2). These results also show that Sakersia africana have
a significant antiradical activity. However, ethanolic-
water and ethanol extract were the higher antioxidant
activity with an IC50 respectively 164.21μg/mL and
177.02 mg/mL. Aqueous extract show lower activity
(IC50 = 195.54 mg/mL). In terms of concentrations of
phenolic compounds in different extracts, it appears
that ethanolic-water extract is less rich in total
polyphenols (391.58 mg EGA/g of drug) and more
active (IC50 = 164.21 mg/mL). It is observed probably
result from the fact that the determination by the
Folin-Ciocalteu reagent is not specific to polyphenols.
Other compounds may interact with said reagent and
provide a high level of polyphenols (Catherine et
al.,1996; Ramarathnam et al, 1997). Thus ethanolic
and aqueous extracts are apparently rich while the
actual content of total phenols is low.
Conclusion
The present study was aimed to evaluate the
phytochemical and antiradical allow us to infer that the
use of Sakersia africana traditherapy by people
against various diseases would depend on its relative
wealth in saponins, phenolic compounds (tannins,
Coumarins and flavonoids) and nitrogen (alkaloids)
endowed of pharmacological properties. This
abundance of active plant gives remarkable properties,
which could justify its multiple therapeutic indications
for which it is used traditherapy. These preliminary
results could provide a scientific basis for the research
of new therapeutic molecules. Further pharmacological
investigations allow us to determine precisely the
different biological activities and to evaluate the acute
and subacute Sakersia africana.
Acknowledgements
The authors thank anyone who contributed to the
completion of this work. Especially we thought Mrs
Daniel Ebozogho Metou, Benoit Ndong Mozogho and
Jean-Calixte Nguema Akoué for her contribution
related to the informations on traditional use of the
plant like this Dr Alain Ondo Azi and Pr Crépin Ella
Missang for the complete support throughout the work
with timely and valuable discussions.
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