Thin layer chromatography (TLC) is a technique used to separate and analyze mixtures. It works on the principles of adsorption and partition, separating compounds based on their relative affinities for the stationary and mobile phases. Key aspects of TLC include choosing an adsorbent like silica gel or alumina, preparing thin layers on a plate, activating the adsorbent, applying samples, developing the plate in a solvent, and detecting separated components using UV light or other methods. TLC can be used to check purity, identify unknown compounds, monitor reactions, purify samples, and evaluate other separation processes.
The slides are informative of HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY & its thorough components further its advantages and applications. The comparison of HPLC and HPTLC is explained.
It is instrumental analytical technique. it is one of the major type of chromatography technique. its basic principle is adsorption. it has many applications in various fields
Pilot plant scale-up is a branch of the pharma companies in which a lab-scale formula is converted into a commercially viable product by creating a reliable manufacturing technique. The same techniques employed in dosage form Research and Development are adapted to multiple output volumes, frequently larger than those obtained during Research and Development. There is always a requirement for an intermediate batch scale describing techniques and imitating those in commercial manufacturing in any new or established pharmaceutical sector. This is accomplished by testing the formulaâs ability to survive batch-scale and process changes.
The slides are informative of HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY & its thorough components further its advantages and applications. The comparison of HPLC and HPTLC is explained.
It is instrumental analytical technique. it is one of the major type of chromatography technique. its basic principle is adsorption. it has many applications in various fields
Pilot plant scale-up is a branch of the pharma companies in which a lab-scale formula is converted into a commercially viable product by creating a reliable manufacturing technique. The same techniques employed in dosage form Research and Development are adapted to multiple output volumes, frequently larger than those obtained during Research and Development. There is always a requirement for an intermediate batch scale describing techniques and imitating those in commercial manufacturing in any new or established pharmaceutical sector. This is accomplished by testing the formulaâs ability to survive batch-scale and process changes.
Instrumentation of Thin Layer ChromatographyTanmoy Sarkar
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Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a system on which a material called the stationary phase is fixed.
Thin-layer chromatography is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of an inert substrate such as glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose.
Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or cellulose.
Ion-Pair chromatography is an alternative to ion exchange chromatography.
ion pair reagent.
Mechanism of ion pair chromatography.
Factors influencing retention.
experimental conditions.
Instrumentation of Thin Layer ChromatographyTanmoy Sarkar
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Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a system on which a material called the stationary phase is fixed.
Thin-layer chromatography is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of an inert substrate such as glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose.
Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or cellulose.
Ion-Pair chromatography is an alternative to ion exchange chromatography.
ion pair reagent.
Mechanism of ion pair chromatography.
Factors influencing retention.
experimental conditions.
High performance liquid chromatography (HPTLC) presentation by using case study "Bioautographic method for selective description of the antioxidant and alpha amylase inhibitory in activity inplant extracts".
All the basic thing of hptlc is explained. The diffrences between tlc ,hplc and hptlc is expalin.All tools of hptlc with images is explained.
High Performance Thin Layer ChromatographyPUNEET NIRMAL
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HPTLC is a most versatile technique and is known for uniformity, purity profile, assay values and precision and accuracy of results. It can handle several samples of even different nature and composition. HPTLC is a modern analytical separation method with extensive versatility, although already much utilized, is still with great potential for future development in research and development.
chromatography, principle, adsorbent of TLC, mobile phase of TLC, techniques in TLC, preparation of TLC plate, standards for TLC, advantages, disadvantages of TLC, Application of TLC.
Thin layer chromatography technique - easier, cheaper.
Handling is easy. Used as an identification test also purity test. It comprises of stationary and mobile phase. There are various types of chromatography technique. TLC consists of three steps - spotting, development, and visualization. The Rf value is used to quantify the movement of the materials along the plate. Rf is equal to the
distance traveled by the substance divided by the distance traveled by the solvent. Its value is
always between zero and one. A TLC analysis might be summarized something like, "Using a silica
gel plate and ethyl acetate as the development solvent, unknown mixture X showed three spots
having Rf's of 0.12, 0.25, and 0.87". CThere are three components in TLC:
(1) the TLC plate (stationary phase), the development solvent (mobile phase), and the sample to be
analyzed (solute). In our experiment the TLC plate consists of a thin plastic sheet covered with a
thin layer of silica gel.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.SĂŠrgio Sacani
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The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Multi-source connectivity as the driver of solar wind variability in the heli...SĂŠrgio Sacani
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The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASAâs Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly AlfvĂŠnic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5âau
but are applicable to near-Earth observatories.
Richard's aventures in two entangled wonderlandsRichard Gill
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Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leberâs hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendelâs laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four Oâclock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
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As consumer awareness of health and wellness rises, the nutraceutical marketâwhich includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutritionâis growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Observation of Ioâs Resurfacing via Plume Deposition Using Ground-based Adapt...SĂŠrgio Sacani
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Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Ioâs surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Ioâs trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Ioâs surface using adaptive
optics at visible wavelengths.
2. Learning objectives:
After this presentation learner should be
able to
ďś Explain principles, Experimental
Details and applications of TLC.
ďś Perform TLC Experiment for Mixture
separation and Purification.
3. Principle
⢠Based on the principle of separation.
⢠Separation is either by Adsorption or by
Partition.
⢠Different relative affinity of compounds
towards SP and MP.
4. Experimental Details
1) Choice of adsorbent
2) Preparation of thin layers in plates
3) Activation of adsorbent
4) Purification of silica Gel G Layer
5) Sample application
6) Development Tank
7) Solvent system (Mobile Phase)
8) Plate development
9) Detection of components
10)Evaluation of chromatogram
6. Choice of adsorbent
Adsorbent Nature Activity Separation
Mechanism
Components to
be separated
Silica Gel Acidic Active Adsorption Acidic and
Neutral
Alimina Basic Active Adsorption Basic and
neutral
Kiesleguhr Neutral Inactive Partition Strongly
hydrophilic
Cellulose
Powder
Neutral None Partition Water Soluble
7. Preparation of thin layers in plates
I. Pouring- Pour adsorbent slurry on plate, Then
Plate is tipped back and forth.
II. Dipping- Plates are dipping at a time back to
back in CHCl3 slurries of adsorbent.
III. Spraying- A small point sprayer for Spread of
the slurry on glass plate.
8. Preparation of thin layers in plates
IV. Spreading- The slurry with the help of
applicator.
V. Precoated plates- Ready to use thin layers
Plates are now available.
9. Activation of adsorbent
⢠Drying the thin layer plates, for 30 minutes in
air and then in an oven at 110 C for another
30 minutes.
⢠For very active layers, can be heated to 150 C
for about 4 hours.
10. Purification of silica Gel G Layer
ďźpreliminary development of plate with
methanol- conc.HCl ( 9:1 v/v)
ďźplates are again dried and activated at 110c
11. Sample application
ďAgla micro syringe is generally used for
quantitative work
ďcapillary tubes may be used for qualitative
work.
13. Solvent system (Mobile Phase)
The choice of the mobile phase is depends upon
the following factors:-
1. Nature of the substance to be separated
2. Nature of the stationary phase used
3. Mode of chromatography ( Normal phase
or reverse phase)
4. Separation to be achieved- Analytical
or preparative.
15. Plate development
⢠Generally Ascending method is used to greater
extent
⢠Descending TLC and Horizontal TLC is also
possible.
⢠These two techniques have no advantages
over the ascending techniques in terms of
efficiency of separation and speed of analysis.
16. Detection of components
A. Allow solvent toevaporate
from surface of TLCplate.
C. Mark spots with a pencil while
viewing under UV.
UV
B. View results under UV light. look
for grayish spots on the
fluorescent greenbackground
UV
17. Evaluation of chromatogram
A. Qualitative Evaluation
B. Quantitative Analysis
B(i) Direct Method: performed directly on the
adsorbent layer
B(ii) Indirect Method: the separated constituents
are quantitatively removed from the adsorbent
and subsequently estimated after elution.
18. Evaluation of chromatogram
B (1) Direct Method:
(i) Measurement of Spot-areas
(ii) Densitometry
(iii) Spectrophotometry
B(2) Indirect Method: These methods are based on
elution techniques, followed by micro-analysis of
the resultant eluate either by
Colorimetry Fluorimetry ; Radiometry ; Flame-
photometry ; UV-Spectrophotometry ;
Gravimetry ; Polarography
19. Applications of TLC
⢠To check the purity of the given samples.
⢠Identification of compounds like acids,
alcohols, proteins, alkaloids, amines,
antibiotics, and more.
⢠To evaluate the reaction process.
⢠To purify samples
⢠To keep a check on the performance of other
separation processes.