Transmission electron microscopy allows the study of inner structures at a very fine scale. It has powerful magnification and resolution, providing high-quality, detailed images. However, TEMs are large, expensive machines that require specialized training to operate and maintain. They also require laborious sample preparation and can only image electron-transparent samples. In medicine, TEM is used as a diagnostic tool in renal biopsies and cancer research to study tumor cell ultrastructure. It also helps toxicology research on the impacts of pollution.

1. BY,
VELLAICHAMY P
MSC BIOINFORMATICS
MSFBI1724

2. TRANSMISSION &
SCANNING ELECTRON
MICROSCOPE

4. Transmission Electron microscopy
INTRODUCTION
 In 1931, while conducting research for his
masters at the technical college of Berlin, Ernst
Ruska and Max Knoll designed first TEM.
 Electron Microscopes are scientific instruments that
use a beam of highly energetic electrons to examine
objects on a very fine scale.
Mainly 2 types:
Transmission Electron Microscope (TEM) -allows
one the study of the inner structures

6. PARTS OF TEM

7. ADVANTAGES &
DISADVANTAGES OF TEM
Advantages
TEMs offer very powerful magnification and
resolution.
TEMs have a wide-range of applications and can be
utilized in a variety of different scientific, educational
and industrial fields
TEMs provide information on element and compound
structure .
Images are high-quality and detailed

8. Disadvantages
 TEMs are large and very expensive.
 Laborious sample preparation.
 Operation and analysis requires special training.
 Samples are limited to those that are electron
transparent.
 TEMs require special housing and maintenance.
 Images are black and white

9. In medicine as a diagnostic tool –important in
renal biopsies.
Cancer research -studies of tumor cell ultra
structure .
Toxicology–to study the impacts of environmental
pollution on the different levels of biological
organization.
BIOLOGICAL APPLICATIONS
TEM