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Comparison of harvesting performances using three different flocculation methods Reporter  : Rifka Aisyah Advisor  : Professor Jo-Shu Chang Date  : 2011.05.18
Flocculation Experiment Condition Combined flocculation using AlCl 3  and DYU500 bioflocculant Condition Description Strain Chlorella vulgaris  ESP-31 Medium Basal Nitrate 0.25 N Carbon source 2% CO 2 ; 0.2 vvm Flocculation method AlCl 3  addition followed by DYU500 addition Initial biomass concentration 1.95 g/L – 2.46 g/L Cultivation day 18 days Working volume (mL) 1000 mL Initial pH 6.65 – 6.71 Lipid content (%) (to be tested)
Microalga growth on basal medium Criteria Description Species Chlorella vulgaris  ESP-31 Culture volume 5 L Agitation rate 150 rpm Cultivation day 18 days The highest biomass concentration 2.10 g/L
Comparison of large scale harvesting performance under different flocculation methods   Initial sedimentation rate AlCl 3  addition pH adjustment Combined flocculation Higher Twofold higher Threefold higher Microalga concentration (g/L) AlCl 3  concentration (g/L) Initial sedimentation rate (%/min) 1.92 – 2.11  0.0 0.21 0.5 3.81 0.7 9.08 Microalga concentration (g/L) pH value Initial sedimentation rate (%/min) 1.92 – 2.35 7.08 0.10 11.00 2.58 12.00 6.28 Microalga concentration (g/L) Condition Initial sedimentation rate (%/min) 1.95 – 2.46 Blank 0.85 0.50 g/L AlCl 3  + 0.20 g/L PGA 11.19 0.70 g/L AlCl 3  + 0.20 g/L PGA 18.30
Comparison of large scale harvesting performance under different flocculation methods The highest flocculation efficiency ,[object Object],AlCl 3  addition pH adjustment Combined flocculation Higher The lowest Microalga concentration (g/L) AlCl 3  concentration (g/L) The highest flocculation efficiency (%) b 1.92 – 2.11  0.0 (Blank) 8.52 0.5 64.49 0.7 88.32 Microalga concentration (g/L)  pH value The highest flocculation efficiency (%) 1.92 – 2.35 7.08 (Blank) 8.32 11.00 55.77 a  (54.01% when settling time = 2 h) 12.00 81.41 a  (73.19% when settling time = 2 h) Microalga concentration (g/L) Condition The highest flocculation efficiency (%) b 1.95 – 2.46 Blank 8.74 0.50 g/L AlCl 3  + 0.20 g/L PGA 42.18 0.70 g/L AlCl 3  + 0.20 g/L PGA 60.42
Comparison of large scale harvesting performance under different flocculation methods Biomass recovery ,[object Object],AlCl 3  addition pH adjustment Combined flocculation About the same, but longer time The lowest Microalga concentration (g/L) AlCl 3  concentration (g/L) Biomass recovery (%) b 1.92 – 2.11  0.0 0.00 0.5 52.48 0.7 86.92 Microalga concentration (g/L) pH value Biomass recovery (%) a 1.92 – 2.35 7.08 0.00 11.00 60.62 12.00 83.78 Microalga concentration (g/L) Condition Biomass recovery (%) b 1.95 – 2.46 Blank 0.00 0.50 g/L AlCl 3  + 0.20 g/L PGA 30.39 0.70 g/L AlCl 3  + 0.20 g/L PGA 57.83
Dose of chemicals and bioflocculant required for each flocculation method The price of NaOH was about one-thirds of the price of AlCl 3  per gram. It can be concluded that flocculation using AlCl 3  was more expensive than flocculation using pH adjustment pH adjustment method pH value NaOH concentration (g/L) 11.00 0.68 12.00 1.44 AlCl 3  addition method AlCl 3  concentration (g/L) 0.50 0.70 Combined method AlCl 3  concentration (g/L) DYU500 bioflocculant (g/L) 0.50 0.20 0.70 0.20
Overall performance assessment of different flocculation methods ,[object Object],[object Object],Parameter Ideal method pH adjustment method AlCl 3  addition method Consecutive treatment using AlCl 3  and bioflocculant Initial sedimentation rate High  Moderate High Very high The highest flocculation efficiency a High Moderate High Low Harvesting time b Low/short Moderate Low/short High Amount of chemicals required Low Moderate High High Cost Low Moderate High High
Time Flocculation efficiency (%) AlCl 3  addition pH adjustment AlCl 3  + DYU500  bioflocculant ,[object Object],[object Object],[object Object],Addition of AlCl 3  caused  to drop  in solution pH . On the other hand,  pH around 7  was shown to be  optimum  in achieving the largest flocculation efficiency by DYU500 from  Bacillus subtilis  DYU1.  Flocculation efficiency could be  restored by adjusting pH of microalga culture to 7 .
Future work ,[object Object],[object Object]

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Team meeting

  • 1. Comparison of harvesting performances using three different flocculation methods Reporter : Rifka Aisyah Advisor : Professor Jo-Shu Chang Date : 2011.05.18
  • 2. Flocculation Experiment Condition Combined flocculation using AlCl 3 and DYU500 bioflocculant Condition Description Strain Chlorella vulgaris ESP-31 Medium Basal Nitrate 0.25 N Carbon source 2% CO 2 ; 0.2 vvm Flocculation method AlCl 3 addition followed by DYU500 addition Initial biomass concentration 1.95 g/L – 2.46 g/L Cultivation day 18 days Working volume (mL) 1000 mL Initial pH 6.65 – 6.71 Lipid content (%) (to be tested)
  • 3. Microalga growth on basal medium Criteria Description Species Chlorella vulgaris ESP-31 Culture volume 5 L Agitation rate 150 rpm Cultivation day 18 days The highest biomass concentration 2.10 g/L
  • 4. Comparison of large scale harvesting performance under different flocculation methods Initial sedimentation rate AlCl 3 addition pH adjustment Combined flocculation Higher Twofold higher Threefold higher Microalga concentration (g/L) AlCl 3 concentration (g/L) Initial sedimentation rate (%/min) 1.92 – 2.11 0.0 0.21 0.5 3.81 0.7 9.08 Microalga concentration (g/L) pH value Initial sedimentation rate (%/min) 1.92 – 2.35 7.08 0.10 11.00 2.58 12.00 6.28 Microalga concentration (g/L) Condition Initial sedimentation rate (%/min) 1.95 – 2.46 Blank 0.85 0.50 g/L AlCl 3 + 0.20 g/L PGA 11.19 0.70 g/L AlCl 3 + 0.20 g/L PGA 18.30
  • 5.
  • 6.
  • 7. Dose of chemicals and bioflocculant required for each flocculation method The price of NaOH was about one-thirds of the price of AlCl 3 per gram. It can be concluded that flocculation using AlCl 3 was more expensive than flocculation using pH adjustment pH adjustment method pH value NaOH concentration (g/L) 11.00 0.68 12.00 1.44 AlCl 3 addition method AlCl 3 concentration (g/L) 0.50 0.70 Combined method AlCl 3 concentration (g/L) DYU500 bioflocculant (g/L) 0.50 0.20 0.70 0.20
  • 8.
  • 9.
  • 10.