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Rifka Aisyah 1 Kuei-Ling Yeh 1 , Chun-Yen Chen 1,2  and Jo-Shu Chang 1,2,3 *   1 Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan 2 Sustainable Environment Research Center, National Cheng Kung University, Tainan, Taiwan 3 Center for Biosciences and Biotechnology, National Cheng Kung University, Tainan, Taiwan NSC 98-2221-E-006-240-MY3 Harvesting  Chlorella vulgaris  ESP-31 for Biofuels Production by Flocculation with Coagulant Addition Date 2010.06.26
BACKGROUND ,[object Object],[object Object],[object Object]
BACKGROUND (2) ,[object Object],[object Object],[object Object],[object Object]
Microalgae as The Third Generation of Biofuels ,[object Object],[object Object],[object Object]
Challenges in Microalgal Harvesting ,[object Object],[object Object],[object Object],20-30%   of the total cost
PURPOSE OF STUDY ,[object Object],[object Object],[object Object],[object Object]
MATERIALS AND METHODS ,[object Object],[object Object],[object Object],Criteria Basal medium Working volume 4 L Agitation rate (rpm) 150 rpm Carbon source CO 2 Carbon source concentration 2% CO 2 ; 0.2 vvm
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],MATERIALS AND METHODS (2)
VARIATION Effect of AlCl 3  concentration Effect of initial biomass concentration Basal medium ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],40 ml 3 cm from surface water 2/3 from bottom of tube 1/2 from bottom of  tube
40 ml [Initial biomass] = 0.81 g/l [Initial biomass] = 1.60 g/l [Initial biomass] = 2.40 g/l [Initial biomass] = 3.22 g/l [Initial biomass] = 4.00 g/l AlCl 3  stock solution Blank 0.1 g/l 0.2 g/l 0.3g/l 0.5 g/l pH = 7.00 3 cm from surface water 2/3 from bottom of tube 1/2 from bottom of tube Cell density Measurement using UV (Wavelength = 688 nm) 0.7 g/l 0.9 g/l 0.7 g/l 1.1 g/l 0.7 g/l 0.9 g/l 0.7 g/l 0.9 g/l 1.1 g/l 1.3 g/l EXPERIMENTAL PROCEDURES 3 min 6 min 10 min . . . 120 min
EXPERIMENTAL RESULTS Microalgae Growth on Basal Medium ,[object Object],increase 12.10% 15.80% 16.67% 21.89% Strain Chlorella vulgaris  ESP-31 Medium Basal CO 2  concentration 2% 0.2 vvm Working volume 4L
[object Object],EXPERIMENTAL RESULTS Effect of AlCl 3  dosage on flocculation performance at OD 688, initial  = 4.07 ) ( Initial biomass concentration = 0.81 g/l) Experimental condition OD 688 ,  initial   (Biomass concentration) 4.07  (0.81 g/l) Cultivation day (days) 32 Lipid content (%) 21.89 AlCl 3  concentration (g/l) Highest flocculation efficiency (%) Specific flocculation rate (%/min) 0 23.83 0.53 0.2 97.79 10.67 0.3 97.54 10.65 0.5 96.07 11.18
[object Object],EXPERIMENTAL RESULTS   Effect of AlCl 3  dosage on flocculation performance at OD 688, initial  = 8.00   (Initial biomass concentration = 1.60 g/l) Need more coagulant to coagulate AlCl 3  concentration (g/l) Highest flocculation efficiency (%) Specific flocculation rate (%/min) 0 32.25 0.10 0.2 22.00 0.23 0.3 63.75 1.53 0.5 98.50 27.79 0.7 98.25 29.29 Experimental condition OD 688 ,  initial   (Biomass concentration) 8.00  (1.60 g/l) Cultivation day (days) 32 Lipid content (%) 21.89
[object Object],EXPERIMENTAL RESULTS Effect of AlCl 3  dosage on flocculation performance at OD 688, initial  = 12.00   (Initial biomass concentration = 2.40 g/l) AlCl 3  concentration (g/l) Highest flocculation efficiency (%) Specific flocculation rate (%/min) 0 26.50 0.94 0.1 40.67 1.14 0.2 27.50 1.33 0.3 26.00 1.22 0.5 98.58 14.90 0.7 99.42 15.53 0.9 98.83 30.32 Experimental condition OD 688 ,  initial   (Biomass concentration) 12.00  (2.40 g/l) Cultivation day (days) 32 Lipid content (%) 21.89
[object Object],EXPERIMENTAL RESULTS Effect of AlCl 3  dosage on flocculation performance at OD 688, initial  = 16.10   (Initial biomass concentration = 3.22 g/l) AlCl 3  concentration (g/l) Highest flocculation efficiency (%) Specific flocculation rate (%/min) 0 18.88 0.89 0.1 38.14 1.20 0.2 44.60 1.45 0.3 37.27 1.12 0.5 54.41 0.61 0.7 99.38 8.90 0.9 99.53 13.37 1.1 99.41 11.57 Experimental condition OD 688 ,  initial   (Biomass concentration) 16.10  (3.22 g/l) Cultivation day (days) 32 Lipid content (%) 21.89
[object Object],EXPERIMENTAL RESULTS  Effect of AlCl 3  dosage on flocculation performance at OD 688, initial  = 20.00   (Initial biomass concentration = 4.00 g/l) AlCl 3  concentration (g/l) Highest flocculation efficiency (%) Specific flocculation rate (%/min) 0 52.36 1.84 0.1 48.43 2.85 0.2 55.71 3.08 0.3 50.39 5.18 0.5 76.38 5.51 0.7 99.66 10.38 0.9 99.78 22.39 1.1 99.63 22.30 1.3 99.56 26.90 Experimental condition OD 688 ,  initial   (Biomass concentration) 20.00  (4.00 g/l) Cultivation day (days) 32 Lipid content (%) 21.89
COMPARISON OF FLOCCULATION PERFORMANCE AT DIFFERENT INITIAL BIOMASS CONCENTRATION Initial OD 688 Initial biomass concentration (g/l) Dose of AlCl 3  needed to obtain at least 90% flocculation efficiency (g/l) Specific flocculation rate needed to obtain at least 90% flocculation efficiency (%/min) 4.07  0.81 0.2 10.67 8.00 1.60 0.5 27.79 12.00  2.40 0.5 14.90 16.10 3.22 0.7 8.90 20.00 4.00 0.7 10.38
CONCLUSIONS ,[object Object],[object Object],[object Object]
Thank you for your attention!!

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Jsc revised rifka-presentation for-990626_(revised[1]

  • 1. Rifka Aisyah 1 Kuei-Ling Yeh 1 , Chun-Yen Chen 1,2 and Jo-Shu Chang 1,2,3 *   1 Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan 2 Sustainable Environment Research Center, National Cheng Kung University, Tainan, Taiwan 3 Center for Biosciences and Biotechnology, National Cheng Kung University, Tainan, Taiwan NSC 98-2221-E-006-240-MY3 Harvesting Chlorella vulgaris ESP-31 for Biofuels Production by Flocculation with Coagulant Addition Date 2010.06.26
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  • 10. 40 ml [Initial biomass] = 0.81 g/l [Initial biomass] = 1.60 g/l [Initial biomass] = 2.40 g/l [Initial biomass] = 3.22 g/l [Initial biomass] = 4.00 g/l AlCl 3 stock solution Blank 0.1 g/l 0.2 g/l 0.3g/l 0.5 g/l pH = 7.00 3 cm from surface water 2/3 from bottom of tube 1/2 from bottom of tube Cell density Measurement using UV (Wavelength = 688 nm) 0.7 g/l 0.9 g/l 0.7 g/l 1.1 g/l 0.7 g/l 0.9 g/l 0.7 g/l 0.9 g/l 1.1 g/l 1.3 g/l EXPERIMENTAL PROCEDURES 3 min 6 min 10 min . . . 120 min
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  • 17. COMPARISON OF FLOCCULATION PERFORMANCE AT DIFFERENT INITIAL BIOMASS CONCENTRATION Initial OD 688 Initial biomass concentration (g/l) Dose of AlCl 3 needed to obtain at least 90% flocculation efficiency (g/l) Specific flocculation rate needed to obtain at least 90% flocculation efficiency (%/min) 4.07 0.81 0.2 10.67 8.00 1.60 0.5 27.79 12.00 2.40 0.5 14.90 16.10 3.22 0.7 8.90 20.00 4.00 0.7 10.38
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  • 19. Thank you for your attention!!

Editor's Notes

  1. Good morning everyone, my name is Rifka. I am from National Cheng Kung University. My advisor is Professor Jo-Shu Chang. Now, I would like to present our experiment about…
  2. The, we increased the initial biomass concentration to … … … .. Because we think that algal suspension need more coagulant when its concentration was increased, then we add more coagulant to the suspension.
  3. This page showed us the effect of AlCl3 dosage on flocculation performance when initial biomass concentration was controlled at 0.81 g/l. We can see that both flocculation rate and highest flocculation rate increased as AlCl3 concentration was increased. However, by simply adding 0.2 g/l AlCl3 we can obtained more than 90% flocculation efficiency.
  4. This page showed us the flocculation performance when initial OD was 1.60 g/l. The trend of both specific flocculation rate and highest flocculation eff increased when the AlCl3 concentration increased. However, to get 90% flocc eff we need to add 0.5 g/l AlCl3, which means we need more coagulant to coagulate