This study investigated using chemical flocculation with aluminum chloride (AlCl3) to harvest the microalgae Chlorella vulgaris ESP-31. The effects of initial biomass concentration and AlCl3 dosage on flocculation efficiency were examined. Results showed that higher AlCl3 dosages and initial biomass concentrations improved flocculation efficiency. At least 90% flocculation required higher AlCl3 dosages and flocculation rates for higher initial biomass concentrations. AlCl3 was found to be an efficient coagulant for recovering oil-rich C. vulgaris biomass.