Предавање др Душана Мишевића о синтетичкој биологији.

1. Synthetic biology
www.dule.frdule@alife.org
Dušan Mišević
Petnica, 26. 3. 2016.

2. "an emerging discipline that uses engineering principles to
design and assemble biological components"
Wellhausen, Rachel, and Kenneth A. Oye. "Intellectual Property
and the Commons in Synthetic Biology: Strategies to Facilitate
an Emerging Technology." Science, Technology and Innovation
Policy, 2007 Atlanta Conference on. IEEE, 2007.

3. "an emerging discipline that uses engineering principles to
design and assemble biological components"
Wellhausen, Rachel, and Kenneth A. Oye. "Intellectual Property
and the Commons in Synthetic Biology: Strategies to Facilitate
an Emerging Technology." Science, Technology and Innovation
Policy, 2007 Atlanta Conference on. IEEE, 2007.
"designing and constructing biological modules, biological
systems, and biological machines for useful purposes."
Nakano, Tadashi (2013). Molecular Communication.
Cambridge.

4. "an emerging discipline that uses engineering principles to
design and assemble biological components"
Wellhausen, Rachel, and Kenneth A. Oye. "Intellectual Property
and the Commons in Synthetic Biology: Strategies to Facilitate
an Emerging Technology." Science, Technology and Innovation
Policy, 2007 Atlanta Conference on. IEEE, 2007.
"designing and constructing biological modules, biological
systems, and biological machines for useful purposes."
Nakano, Tadashi (2013). Molecular Communication.
Cambridge.
“design and construction of novel artificial biological pathways,
organisms or devices, or the redesign of existing natural
biological systems."
http://www.synbioproject.org/topics/synbio101/definition/

5. Synthetic biology
Genetic engineering

8. Common circuit diagram
symbols (US symbols)
Synthetic Biology Open Language
(SBOL) standard visual symbols for
use with BioBricks Standard

12. Gardner, Timothy S., Charles R.
Cantor, and James J. Collins.
"Construction of a genetic toggle
switch in Escherichia coli." Nature
403.6767 (2000): 339-342.

14. Number of Regions: 5
Number of teams: 280
Number of participants: 5018

15. The Edinburgh University
2009 iGEM Team
The Edinburgh University 2009 iGEM Team

22. The Edinburgh University
2009 iGEM Team
https://playingwithbacteria.wordpress.com/20
12/05/02/five-tips-for-coming-up-with-a-
winning-igem-project/
Five tips for coming up with a winning
iGEM project
1. Start from a problem
2. Start from an advance or discovery
3. Find something cool, then add ‘Bio’ in front of it, or ‘coli’
to the end of it
4. Start as broad as you can
5. Browse the registry & past teams’ work

26. Craig Venter

27. https://www.youtube.com/
watch?v=HLP6x4h1tOM

28. JCVI-syn3.0
(Image: Tom Deerinck and
Mark Ellisman of the
National Center for
Imaging and Microscopy
Research at the University
of California at San Diego)
Mycoplasma
mycoides
473 genes
149 genes
with unknown
function

30. https://www.youtube.com/
watch?v=SEh7Jh775iM

32. https://www.youtube.com/wat
ch?v=LSXZLs9OS8k

37. Kopi luwak

38. Kopi luwak

41. Unique sugar profile of Cultured Coffee vs control
(liquid and gas chromatography, HPAEC)
Kopi luwak

44. Simpson’s
Paradox

45. 1973 grad school admission UC Berkley

46. 1973 grad school admission UC Berkley

47. Chuang, John S., Olivier Rivoire, and Stanislas Leibler. "Simpson's paradox
in a synthetic microbial system." Science 323.5911 (2009): 272-275.

48. Chuang, John S., Olivier Rivoire, and Stanislas Leibler. "Cooperation and Hamilton's
rule in a simple synthetic microbial system." Molecular systems biology 6.1 (2010).

49. Dimitriu, T., Lotton, C., Bénard-Capelle, J., Misevic, D., Brown, S.P., Lindner, A.B. and
Taddei, F., 2014. Genetic information transfer promotes cooperation in bacteria.
Proceedings of the National Academy of Sciences, 111(30), pp.11103-11108.