Subculturing or passage is the method of preserving or continuing the culture for longer periods Subculturing can be done in three procedures based on the cells we need to passage or subculture. 1. Bacterial subculture. 2.Monolayer sub culture. 3. Suspension sub culture. Requirements :- For any of the sub culture it needs the following criteria The adequate conditions like subculture should be done periodically, need of adding nutrients or shifting of the innoculum both are considered as subculture. 1. MONOLAYER CULTURES when the continous cell lines or other adhered cells which are grown on the roux flask or other surface which are cultured by removing by trypsinization procedures and grown in the fresh medium eg DMEM medium Dulbeco modified Eagles medium. So that the cells will grow vigorously and differentiate. 2. Suspension cultures when cells of the well grown medium is transferred to the fresh medium components so that cells grow in high nutrient concentration, 3. Bacterial sub culturing : Transfer of innoculum(colony) from solid agar surface to broth medium, solid surface to solid surface, liquid broth to liquid broth, liquid broth to solid agar medium surface, sub culturing can be done by streaking, pouring, spreading onto fresh medium components. Solution Subculturing or passage is the method of preserving or continuing the culture for longer periods Subculturing can be done in three procedures based on the cells we need to passage or subculture. 1. Bacterial subculture. 2.Monolayer sub culture. 3. Suspension sub culture. Requirements :- For any of the sub culture it needs the following criteria The adequate conditions like subculture should be done periodically, need of adding nutrients or shifting of the innoculum both are considered as subculture. 1. MONOLAYER CULTURES when the continous cell lines or other adhered cells which are grown on the roux flask or other surface which are cultured by removing by trypsinization procedures and grown in the fresh medium eg DMEM medium Dulbeco modified Eagles medium. So that the cells will grow vigorously and differentiate. 2. Suspension cultures when cells of the well grown medium is transferred to the fresh medium components so that cells grow in high nutrient concentration, 3. Bacterial sub culturing : Transfer of innoculum(colony) from solid agar surface to broth medium, solid surface to solid surface, liquid broth to liquid broth, liquid broth to solid agar medium surface, sub culturing can be done by streaking, pouring, spreading onto fresh medium components..

1. Subculturing or passage is the method of preserving or continuing the culture for longer periods
Subculturing can be done in three procedures based on the cells we need to passage or
subculture.
1. Bacterial subculture.
2.Monolayer sub culture.
3. Suspension sub culture.
Requirements :-
For any of the sub culture it needs the following criteria
The adequate conditions like subculture should be done periodically, need of adding nutrients or
shifting of the innoculum both are considered as subculture.
1. MONOLAYER CULTURES when the continous cell lines or other adhered cells which are
grown on the roux flask or other surface which are cultured by removing by trypsinization
procedures and grown in the fresh medium eg DMEM medium Dulbeco modified Eagles
medium. So that the cells will grow vigorously and differentiate.
2. Suspension cultures when cells of the well grown medium is transferred to the fresh medium
components so that cells grow in high nutrient concentration,
3. Bacterial sub culturing : Transfer of innoculum(colony) from solid agar surface to broth
medium, solid surface to solid surface, liquid broth to liquid broth, liquid broth to solid agar
medium surface, sub culturing can be done by streaking, pouring, spreading onto fresh medium
components.
Solution
Subculturing or passage is the method of preserving or continuing the culture for longer periods
Subculturing can be done in three procedures based on the cells we need to passage or
subculture.
1. Bacterial subculture.
2.Monolayer sub culture.
3. Suspension sub culture.
Requirements :-
For any of the sub culture it needs the following criteria
The adequate conditions like subculture should be done periodically, need of adding nutrients or
shifting of the innoculum both are considered as subculture.
1. MONOLAYER CULTURES when the continous cell lines or other adhered cells which are
grown on the roux flask or other surface which are cultured by removing by trypsinization

2. procedures and grown in the fresh medium eg DMEM medium Dulbeco modified Eagles
medium. So that the cells will grow vigorously and differentiate.
2. Suspension cultures when cells of the well grown medium is transferred to the fresh medium
components so that cells grow in high nutrient concentration,
3. Bacterial sub culturing : Transfer of innoculum(colony) from solid agar surface to broth
medium, solid surface to solid surface, liquid broth to liquid broth, liquid broth to solid agar
medium surface, sub culturing can be done by streaking, pouring, spreading onto fresh medium
components.