2. Introduction
Haberlandt Gottlieb first but
unsuccessful attempt of tissue culture
was made using monocots in 1902
It defined as a collection of experimental
methods of growing the plant cells,
tissues and organs in an artificially
prepared nutrient medium static or
liquid, under aseptic conditions.
3. Discovered
Growth and development in
suspension cultures in 1958 F. C.
Steward and co-workers.
Suspension culture of higher plant
cells in synthetic media were
demonstrated by Torrey in 1965, Early
and Torrey suggested the defined
media for the cells isolated from
convolvulus. Well colonies were
formed on his suggested media.
4. Definition
Suspension culture is a type
of culture in which single cells
or small aggregates of cells
multiply while suspended in
agitated liquid medium.
It is referred to as cell culture
or cell suspension culture.
6. Cell
Suspension
Culture
Cell suspension culture requires
optimization of the cell line, the
cultivation media and the
bioreactor system.
The culture of tissue and cells
cultured in liquid nutrient
medium, producing a suspension
of single cells and cells and cell
clumps.
7. Bath
culture
Bath culture is a type of cell
suspension where the cell
material grows in finite volume
of agitated liquid medium.
the biomass growth in batch
culture follows a fixed pattern.
Cultures are maintained
continuously by sub-culture
8. Continuous Culture
• In continuous culture system, the old
liquid medium is replaced continuously
by the fresh liquid medium to stabilize
the physiological states of the growing
cells.
• There are two types of continuous
cultures as given below;
Closed continuous cultures:
Open continuous cultures:
9. Closed continuous suspension
cultures
• In closed type continuous
suspension cultures, the used
medium is replaced with fresh
medium; therefore, the cells from
used medium are mechanically
taken out and added back to the
culture.
• This results in increase in the
biomass.
10. Open
continuous
suspension
cultures
• In open type continuous cultures both
cells and used medium are replaced
with fresh medium resulting in
maintenance of culture at constant and
sub maximal growth rate.
11. Advantages
The nutrients can be continually
adjusted.
This systems can be scaled for
large scale production of the cells.
A whole plant can be regenerated
from a single plant cell.
12. Disadvantages
• The productivity of suspension cultures
decreases over extended subculture
periods.
• Slow growth and low productivity of plant
cells.
• Cells may get damaged by shear conditions.
