The document discusses synthetic biology and the International Genetically Engineered Machines (iGEM) competition. It summarizes that synthetic biology designs biological components for useful purposes. iGEM is an annual synthetic biology competition where student teams design and build complex biological systems using standard DNA parts called "BioBricks". The document then provides details on Cornell iGEM's 2014 project to engineer E. coli bacteria to uptake heavy metals from water to purify contaminated water sources.
genetic engineering: Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. Many organism are manipulated with the help genetic engineering useful for mankind.
Are you interested in the pGLO™ experiment? Learn more about this hands-on laboratory activity with a FREE download of BioRad’s pGLO™ experiment power point.
genetic engineering: Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. Many organism are manipulated with the help genetic engineering useful for mankind.
Are you interested in the pGLO™ experiment? Learn more about this hands-on laboratory activity with a FREE download of BioRad’s pGLO™ experiment power point.
Basic principles of genetic engineeringSteffi Thomas
Basic principles of genetic engineering, Recombinant DNA, Genetically Modified organism (GMO), Tools used in genetic engineering, restriction endonuclease, DNA ligase, cloning vector, process of genetic engineering, applications of genetic engineering (in animals, plants, human), production of insulin by rDNA technology, gene therapy, possible hazards of genetic engineering
Genetic Engineering By Ghawas khan from AWKUM PharmacyGhawas
Genetic Enginnearing is on one of the emerging branch of life sciences... Tried my best to create a best presentation for you. Remember me in your prayers.
Overview of patenting activity in Syn Bio, patentable subject matter, requirements for patentability and infringement issues for innovations in that field.
The following powerpoint presentation consist of all the essential knowledge about recombinant DNA technology, its techniues and different uses, advantages, disadvantages,
Sample Prep Solutions for Microbiome ResearchQIAGEN
An accurate molecular analysis of the microbial constituents of a particular community is contingent upon high-yielding and non-biased nucleic acid extraction methodologies. Only by ensuring that all species and classes of microorganisms present in a sample are effectively lysed during extraction will one be able to reliably assess the composition of that sample. An additional challenge faced in nucleic acid extraction is the presence of persistent, co-purifying polymerase inhibitors endogenous to one’s sample. This presentation will focus on nucleic acid extraction tools developed by MO BIO Laboratories that facilitate accurate non-biased community analysis and eliminate common amplification problems via the depletion of endogenous polymerase inhibitors using our patented Inhibitor Removal Technology.
Basic principles of genetic engineeringSteffi Thomas
Basic principles of genetic engineering, Recombinant DNA, Genetically Modified organism (GMO), Tools used in genetic engineering, restriction endonuclease, DNA ligase, cloning vector, process of genetic engineering, applications of genetic engineering (in animals, plants, human), production of insulin by rDNA technology, gene therapy, possible hazards of genetic engineering
Genetic Engineering By Ghawas khan from AWKUM PharmacyGhawas
Genetic Enginnearing is on one of the emerging branch of life sciences... Tried my best to create a best presentation for you. Remember me in your prayers.
Overview of patenting activity in Syn Bio, patentable subject matter, requirements for patentability and infringement issues for innovations in that field.
The following powerpoint presentation consist of all the essential knowledge about recombinant DNA technology, its techniues and different uses, advantages, disadvantages,
Sample Prep Solutions for Microbiome ResearchQIAGEN
An accurate molecular analysis of the microbial constituents of a particular community is contingent upon high-yielding and non-biased nucleic acid extraction methodologies. Only by ensuring that all species and classes of microorganisms present in a sample are effectively lysed during extraction will one be able to reliably assess the composition of that sample. An additional challenge faced in nucleic acid extraction is the presence of persistent, co-purifying polymerase inhibitors endogenous to one’s sample. This presentation will focus on nucleic acid extraction tools developed by MO BIO Laboratories that facilitate accurate non-biased community analysis and eliminate common amplification problems via the depletion of endogenous polymerase inhibitors using our patented Inhibitor Removal Technology.
Microbiome Identification to Characterization: Pathogen Detection Webinar Ser...QIAGEN
The research community has begun correlating the makeup of individual microbiomes with disorders and diseases such as autism, atherosclerosis, obesity and cancer. To accomplish this, researchers must first identify and characterize these microbial communities. This slidedeck will begin with a general introduction of metagenomics and an overview of experimental strategies. Following this, a comprehensive microbiome assay pipeline will be introduced. We conclude with application-based examples that demonstrate how to identify and characterize microbiome profiles.
Molecular pathology in microbiology and metagenomicsCharithRanatunga
INTRODUCTION
HISTORY
Steps
Analysis
Metagenomic Process
Sequence-based analysis
Function-based analysis
Application of metagenomics
Future Directions of metagenomics
Examples for metagenomics projects
1. Unveiling the mysteries of synthetic biology with
Presenters: Jonlin Chen, Grace Livermore, Tim Abbott, and Jeffrey Ly
2. WHAT IS SYNTHETIC
BIOLOGY?
Designing and constructing biological devices, networks and
pathways for useful purposes
Creating synthetic components or re-assembling pre-existing genes
Wiring biological circuitry to make:
Biological switches, oscillators, toggle switches
Logic gates, pulse generators
For: Biosensing, therapeutic treatment, biofuels…. And much more
3. WHAT IS IGEM?
• ‘International Genetically Engineered Machines”
• Global synthetic biology competition
• HS / Undergraduate / Graduate / Entrepreneurship
• Students design and build complex biological systems
made from standard, interchangeable DNA parts
“Biobricks”
4. How does one genetically modify
an organism?
Herbert Boyer Robert Swanson
5. • What is a restriction enzyme?
DNA
Restriction Enzyme
• Why do bacteria have these enzymes?
• Immunity against viruses!
• Will the enzymes cut up the bacteria’s DNA?
• Nope! The bacteria is able to protect its own DNA!
6. What Made These Two Interested
in Restriction Enzymes?
Herbert Boyer Robert Swanson
• What if you could cut open bacterial DNA, and insert DNA of
your choice in?
8. History of iGEM
• Started as a spin-off of an MIT
course in 2003
• Has grown from 5 teams in 2004 to
245 in 2013!
• Competition is run by co-founder:
Randy Rettberg
• Established the BioBrick, a
standardized biological part for
easier genetic manipulation
9. How do You Clone with BioBricks?
1. Get some DNA!
5 mL culture
Plasmid DNA
E. coli
10. How do You Clone with BioBricks?
2. Amplify target sequence through PCR
11. How do You Clone with BioBricks?
3. Double-digest backbone and PCR product
12. How do You Clone with BioBricks?
4. Gel purify your digests
13. How do You Clone with BioBricks?
5. De-phosphorylate backbone
14. How do You Clone with BioBricks?
6. Ligate your DNA
15. How do You Clone with BioBricks?
7. Transform into bacteria
16. The Team
● Award-winning
undergraduate project
team
● International Genetically
Engineered Machines
● “Biobricks” : Standardized
genetic parts
○ Ever-expanding registry
● Annual competition
19. “LEAD IT GO” 2014
HEAVY METAL SEQUESTRATION SYSTEM
• Engineering E. coli for the uptake of heavy metals
• Integrate cells into dry lab’s filtration system
• Test efficacy of filter with lab based and local water
supplies
• Building CAD portfolio of designs that allow the system to
scale to many applications
20. Water Contamination
● Heavy metals
o Mercury, lead, nickel
● Sources:
o Industrial manufacturing
o Hydraulic fracturing
o Coal mining
o Construction
● Harmful to humans, ecosystem, aquatic life
o Biomagnification
21. Project Goals
● Engineer E.coli to uptake heavy metals from
the environment
● Integrate cells into a continuous water
filtration system
● Test efficacy of biological filter with lab
based and local water samples
22. Heavy Metal Transport Proteins
● Proteins specific to heavy metals
o nixA - nickel
o merT and merP - mercury
o CBP4 - lead
● Behind the constitutive Anderson promoter
nixA
Anderson
Promoter
23. E.coli - Metal Transport
E.coli
Metal Ions
Transport
Protein
24. Metallothioneins
● Low molecular weight proteins
● High metal binding affinities
o GST-YMT, GST-PMT
● Expression causes cellular dormancy
● Arabinose inducible system
Arabinose
araBAD Promoter
Metallothionein
T7 Promoter
T7 Polymerase
27. Saturation Sensing System
● Insert mRFP gene behind metal-inducible
promoters
o Cells will fluoresce when metallothioneins are
saturated
● Metal sensitive promoters induced once MT
are saturated
29. Efficacy Analysis
● Continuous flow hollow fiber reactor
● Implementation
o Factory waste water outlet
o Contaminated bodies of water
● Testing
o Lab made waste water
o Local water samples
▪ Cayuga Lake
▪ Ithaca Gun factory
30. Fun Activity: Learn to Load Gels!
Purpose:
To determine relative lengths of pieces of DNA
Applications of DNA Electrophoresis
● murder mysteries
● paternity cases
● disease diagnosis