This study developed a novel biodegradable porous scaffold for skin regeneration composed of collagen, hyaluronic acid, and gelatin. The scaffold was tested in vitro by culturing human keratinocytes, melanocytes, and fibroblasts, which all proliferated normally. In vivo tests on rats found the scaffold treated wounds healed faster than untreated wounds, with over 50% closure within 7 days versus only 60% for untreated wounds. Histological analysis further supported the scaffold's ability to facilitate more rapid wound healing and regeneration of skin tissue.