Downloaded 29 times
More Related Content
Similar to Artificial skin
Artificial skin
- 1. ARTIFICIALSKIN Maierdanjiang Wufuer Department ofPlastic and Reconstructive Surgery, Institute of Human-Environment Interface Biology, College of Medicine, Seoul Nat’l University, Seoul, Republic of Korea Biomedical Research Institute, Seoul Nat’l Univ. Hospital, Seoul, Republic of Korea
- 2. Apligraf® Country Objective EpidermisDermis/Matrix graft? FDA&Indication storag USA, Organoge nesis, Inc. Epidermal/ Dermal replacement Keratinocytes from human neonatal foreskin tissue Bovine collage n I gel with all ogeneic NO FDA1998, approved for use in the treatment of diabetic foot ulcers and venous leg ulcers. 37' incubator , viable for 2-3 days differentiati on Application Advantages Disadvantages Price More info Reference 10 days 1.Prepare wound 2.Apply apligraf 3.Dress wound Wound dressing with two different cell types , 3 times faster healin g in difficult wound s/ Most expensive, short shelf-life $1,100. /42 cm 2 a bilaminar structure form ed by type I bovine collag en gel populated by live neonatal fibroblasts coate d with a layer of epithelial cells Wilkins LM, Watson SR, Prosky SJ, et al. Develop ment of a bilayered living skin construct for clinical applications. Biotechnol B ioeng. 1994;43747-756. 2. Parenteau NL, Nolte C M, Bilbo P, et al. Epiderm is generated in vitro: practi cal considerations and app lications. J Cell Biochem. 1991;45:245-251
- 3. OrCel™ Country Objective EpidermisDermis/Matrix STG? FDA&Indication storag USA, Forticell Bioscience Inc., NY Epidermal/ dermal replacement keratinocytes deriv ed from neonatal foreskin, Bovine collage n sponge with allogeneic fibro blasts and kerat inocytes） no 2001, FDA approve , partial-thickness wounds 37' incubator differentiati on Application Advantages Disadvantages Price More info Reference 37' incubato r 1.Prepare wound 2.Apply orcel 3.Dress wound where it provides a f avourable matrix for host cell migration. expensive $1,200/ 44 cm2 OrCel® is a bilayer dress ing resembling normal skin and was developed as a tissue-engineered bi ological dressing. https://www.i-ho rus.com/orcel-te chnology/
- 4. Integra® Country Objective EpidermisDermis/Matrix SG? FDA&Indication storag USA, I ntegra Lif e Sciences Corp., NJ, Dermal replac ement silicone/ Synthetic polysiloxanepolym er/ that acts skin epidermis. It protects the wound from in fection and controls both hea t and moisture loss. The dermal layer is mad e of a 3D porous matrix of cross-linked collagen (pure collagen from co ws) and glycosaminogly can. (glycosaminoglyca n made from shark cartil age). yes FDA In 1996,treatment of life thre atening bur injuries and 2014 the r econstruction of scar contractures. Each sheet of Integ ra template is store d in phosphate buff er within a foil pou ch. Each sterile foil pouch is packaged i n a sealed outer che vron-style pouch. flat at 2°– 30°C. Pr otect from freezing. differenti ation Application Advantages Disadvantages Price More info Reference NO 1.Day 21+: Sil icone Removal 2.Day 21+: epi dermal autogra ft Has the some of the advanta ges of an autograft without a donor site. Once the silicone sheet begins to separate with vascularization of the collage n matrix, it is removed and e ngineered tissue placed over this bed or STSG used In the absence of living cells, it is very difficult to see how one could create a permanent artifi cial dermal barrier. is a bi-layered extracellular matrix of fibers of cross-linked bovine co llagen and chondroitin-6-sulfate (a component of cartilage) with sil icone backing. Once an organized regeneration of dermal tissue is fo rmed(neodermis), the disposable s ilicone sheet is removed and an ul trathin autograft is placed over the neodermis. 1.- I.V. Yannas;"De sign of an artificial skin II: Control of c hemical compositio n."
- 5. Dermagraft® Country Objective EpidermisDermis/Matrix graft? FDA&Indication storag USA, Organo genesis Inc., M A Dermal replaceme nt NO living allogeneic fibro blasts on a polyglactin mesh. YES FDA-approved for diabetic ulcers, treat deeper wounds. In a box /pouch . Preserved at -70° C with 6-month shelf-life different iation Application Advantages Disadvantages Price More info Reference no 1.Prepare wound 2.Apply 3.Dress wound. The polyglactin m esh is biodegradab le and is reabsorbe d after 3 to 4 weeks. include a lack of host immune resp onse, ease of application and resistance to teari ng As Dermagraft has no epidermal component, clinicians have used it with a subsequent appl ication of a split thickn ess skin graft. 2*3CM, $1500 per app lication The fibroblasts come from neonatal foreskin and rem ain viable after implantati on, continuing to secrete matrix proteins and growt h factors. www.dermagraf t.com/home/
- 6. Epicel™ Country Objective EpidermisDermis/Matrix graft? FDA&Indication storag USA , Genzyme Corporation, Cambridge MA, Epidermal cover Cultured substitute: autologous keratinoc ytes with murine fibr oblasts N0 NO superficial wounds. 37' incubator differentiati on Application Advantages Disadvantages Price More info Reference The product is made from a biopsy of t he patient’s o wn skin, whi ch is subsequ ently culture d for three w eeks. Epicel™ consists o f sheets of autolog ous keratinocytes a ttached to a petrola tum gauze support, which is removed a pproximately 1 we ek after grafting The ability to expand epidermal cells in vitr o and produce autolog ous cultured epithelium w as an important breakt hrough in burn therap y include cost and d elay of application due to the three-w eek graft cultivati on. It has no derm al component and is accordingly suit ed for more superf icial wounds. The patient’s own skin cells are grown or cultured from a postage‐stamp sized sampl e of the patient’s own health y skin. The skin cells are gr own on a layer of irradiated mouse cells, making Epicel ® a xenotransplantation pro duct.
- 7. TransCyte™ Country Objective EpidermisDermis/Matrix graft? FDA&Indication storag USA Dermal replacem ent Outer epidermal anal og is a thin nonporou s silicone mesh-like material with barrier functions Inner dermal ana log is layered wit h human fibrobla st products main ly collagen type 1, fibronectin an d Glycosaminogl ycan YES It is typically used to treat second degree burn frozen differentiati on Application Advantages Disadvantages Price More info Reference Human Fibroblast Derived Tempora ry Skin Substitute Temporary woun d covering for sur gically excised fu ll thickness and p artial thickness bu rns. This cryopreserved matrix contains high levels of proteins an d growth factors wit h no viable cells. Need to store fro zen till use Relat ively expensive r equirement of m ultiple applicatio ns Human Fibroblast Derive d Temporary Skin Substit ute Temporary wound coverin g for surgically excised fu ll thickness and partial thi ckness burns. an outer silicon e layer (with no pores)
- 8. Alloderm® Country Objective EpidermisDermis/Matrix graft? FDA&Indication storag USA, LifeC ell Corporat ion, Bridge water, NJ Dermal replacement NO Non-living dermal r eplacement compos ed of human cadave ric skin in which th e epidermis has be en removed by salt processing. yes both acute and chronic wo unds. freeze-dried differentiati on Application Advantages Disadvantages Price More info Reference NO 1.Prepare wou nd 2.Apply 3.Dress wound. of Alloderm include a long shelf life beca use it is freeze drie d, immediate availa bility and minimal risk for host immunologic re sponse. of this product inclu de the risk of diseas e transmission. T here is no epidermal component so clinic ians commonly use the product with a split-thickness skin graft. AlloDerm supports tissue regeneration by allowing r apid revascularization, wh ite cell migration and cell population - ultimately bei ng transformed into host ti ssue for a strong, natural r epair. http://www.bioh orizons.com/allo derm.aspx
- 9. Oasis® Country Objective EpidermisDermis/Matrix graft? FDA&Indication storag USA, Cook Biot ech, Inc. Dermal replac ement NO intact matrix nat urally derived fr om porcine smal l intestinal subm ucosa (SIS), YES partial- and full-thickness wounds, pressure ulcers, v enous ulcers, chronic vasc ular ulcers, Room temperatu re and on-the-sh elf storage/Long 2-year shelf life differentiati on Application Advantages Disadvantages Price More info Reference 1. Prepare 2. Apply 3. Anchor 4. Rehydrate 5. Dress 6. Cover 7. Reassess 8. Reapply every 7 days Reapply OASIS® Wou nd Matrix every 7 days or as needed, If a gel fo rms on the wound surfa ce, do not attempt to for cibly remove. Gently ri nse the wound surface, l eaving the extracellular matrix (ECM) gel intact. Long 2-year sh elf life Minimally processed an d sterilized using a propr ietary method that allow s long-term storage of th e structure without comp romising its ability to su pport the body’s own tis sue repair mechanisms2 http://www.oasis woundmatrix.co m/application-w ound-matrix
- 10. Matriderm® Country Objective EpidermisDermis/Matrix graft? FDA&Indication storag Germany, MedSkin So lutions Dr. Suwelack A G, Billerbec k Dermal replac ement NO matrix containing I, II, and V-type colla gen extracted from b ovine ligament and dermis, and alph a-elastin hydrolysate s. I YES acute burn wounds and sc ar reconstruction procedur es. Room temperatu re differentiati on Application Advantages Disadvantages Price More info Reference It can be appli ed concurrentl y with the split-t hickness skin graft It can be applied co ncurrently with the split-thickn ess skin graft applied together with a split skin graf,, secondary surgery is not required The matrix serves as a sup port structure for the ingro wth of cells and vessels.
- 11. Biobrane® Country Objective EpidermisDermis/Matrix graft? FDA&Indication storag USA Dermal replacement Outer epidermal analog—ultrathin silicone film; inner dermal analog—3D nylon filament with type I collagen peptides YES The treatment is specific t o noninfected partial-thick ness burn wounds and don or site wounds, RT differentiati on Application Advantages Disadvantages Price More info Reference Biobrane is appliedand held in place with special glue . Biobrane will take between 10-14 days. Biobrane has been shown to reduce the pain and discomfort felt when the skin is scaled Temporary wound d ressing that is remo ved when wound is healed or when autograft skin is ava ilable Biobrane™ was first intro duced for the treatment of burn wounds and donor sit es in 1979 11.Smith DJ. Us e of Biobrane in wound managem ent. JBCR May/J une 1995:16(3) Supplement;317- 320.
- 12. Theraskin® Country Objective EpidermisDermis/Matrix graft? FDA&Indication storag USA Epidermal/ dermal replace ment human skin allogr aft, composed of living cells, kerat inocytes human skin allogra ft, fibroblasts and a fully developed ex tra cellular matrix (ECM) in its epider mis and dermis laye rs. NO non-healing chronic woun d TheraSkin is cry opreserved (-70º C) differentiati on Application Advantages Disadvantages Price More info Reference Landsman, A. (2010). A retrospect ive clinical study of con secutive pat ients to exa mine the eff ectiveness 1.Prepare wound 2.Apply 3.Dress wound. Provides a broad spectrum of the major growth fa ctors for wound healing a biologically active, cryo preserved human skin allo graft with both epidermis and dermis layers. Its cell ular and extracellular com position provides a supply of growth factors, cytokin es and collagen to promot e wound healing. https://www.inov a.org/upload/doc s/Healthcare%20 Services/Wound- Healing/retrospe ctive-study2.pdf
- 13. Epicel® (Genzyme TissueRepair). Epicel is a cultured autograft composed of living keratinocytes. The product is made from a biopsy of the patient’s own skin, which is subsequently cultured for three weeks. It has no dermal component and is accordingly suited for more superficial wounds. In addition to serving as a permanent skin replacement. It can also cover a large wound area. Disadvantages include cost and delay of application due to the three-week graft cultivation.
- 14. 14 Alloderm® (Life Cell) Alloderm is an acellular, Non-living dermal replacement composed of human cadaveric skin in which the epidermis has been removed by salt processing. The product is freeze-dried and has been used in both acute and chronic wounds. There is no epidermal component so clinicians commonly use the product with a split-thickness skin graft. •Advantages of Alloderm include a long shelf life because it is freeze dried, immediate availability and minimal risk for host immunologic response. •Disadvantages of this product include the risk of disease transmission.
- 15. 15 Matriderm® Is anacelular dermal substuitutes is a structurally intact matrix of bovine I, II, and V- type collagen with elastin. It is utilised for dermal regeneration. Its indications are full thickness or deep dermal burn wounds and chronic wounds. The matrix serves as a support structure for the ingrowth of cells and vessels. Its elastin component improves the stability and elasticity of the regenerating tissue. (Skin and Health Care AG, Billerbeck, Germany)