Silica gel column chromatography is a technique used for separating and purifying liquid and solid compounds by adsorbing unwanted substances with silica gel and activated aluminum oxide. It offers high separation efficiency, reproducible performance, and various applications including pharmaceuticals, environmental testing, and biotechnology. Different types of silica gel, characterized by their mesh sizes and properties, optimize this method for various analytical purposes.

1. SILICA GEL COLUMN CHROMATOGRAPHY

2. SILICA GEL COLUMN CHROMATOGRAPHY
Column chromatography is used for
separating and purifying the liquids
and solid compounds. It is an
effective chromatography technique
in which stationary phase adsorbs
the unwanted compounds and other
impurities using silica gel and
activated aluminum oxide powder as
powerful adsorbents

3. Benefits of Silica Gel Column Chromatography
• Optimize separation efficiency
• High adsorption capacity with large surface
area
• Reproducible performance
• Suitable for large scale applications
• Excellent flow rates
• Direct scalability

4. General uses of chromatography in our real
life are
• Pharmaceutical Company –determine amount
of each chemical found in new product.
• Hospital –detect blood or alcohol levels in a
patient’s blood stream.
• Law Enforcement – to compare a sample found
at a crime scene to samples from suspects.
• Environmental Agency – determine the level of
pollutants in the water supply.
• Manufacturing Plant – to purify a chemical
needed to make a product.
• Biotechnology industry – establishing the purity
or concentration of compounds in
biotechnological research.
• Biological application – Chromatography has
many applications in biology. It is used to
separate and identify amino acids,
carbohydrates, fatty acids, and other natural
substances.
• Environmental testing laboratories use
chromatography to identify trace quantities of
contaminants such as PCBs in waste oil and
pesticides such as DDT in groundwater.

5. Application of Column Chromatography
• Analytical uses: For analytical purposes,
column chromatography finds limited
applications. Vestergaard and Sayegh could
separate seven urinary steroids within 5
hours which requires 36 hours on a normal
column. They have used narrow Teflon
tubing packed with aluminum oxide or
silica gel.
• Separation of geometrical isomers: The
separation of cis/trans isomer is based on
the steric factors. Isomers whose functional
groups can approach the surface of the
adsorbent more easily are more strongly
adsorbed.
• Separation of Diastereomers
• Separation of tautomeric mixtures.
• Separation of racemates.

6. Types of silica gel for column chromatography mesh size.
SILICA GEL 60-120 MESH
Related Categories: Flash Chromatography,
TLC
Grade: Laboratory Grade
Particle Size: 60-120 Mesh (250-
125μm)
Boiling Point: 2230 0C
Melting Point: >1600 0C
Bulk Density KG/LIT 500-650
Ph 10% aq solution 7 ± 0.5
Fe (Iron) <75 ppm
Chloride(NaCl) Max 300ppm
Pore Diameter –A 40-60A
Surface Area m2/gm 350-450
Moisture Content % <6.00

7. SILICA GEL 60-200 MESH
Related Categories: Flash
Chromatography, TLC
Grade: Laboratory Grade
Particle Size: 60-200 Mesh (250-
74μm)
Boiling Point: 2230 0C
Melting Point: >1600 0C
Bulk Density KG/LIT 545-620
Ph 10% aq solution 7 ± 0.5
Fe (Iron) <75 ppm
Chloride(NaCl) Max 300ppm
Pore Diameter –A 40-60A
Surface Area m2/gm 350-450
Moisture Content % <5.00

8. SILICA GEL 70-230 MESH
Related Categories: Flash
Chromatography, TLC
Grade: Laboratory Grade
Particle Size: 70-230 Mesh (210-
63μm)
Boiling Point: 2230 0C
Melting Point: >1600 0C
Bulk Density KG/LIT 500-650
Ph 10% aq solution 7 ± 0.5
Fe (Iron) <75 ppm
Chloride(NaCl) Max 300ppm
Pore Diameter –A 40-60A
Surface Area m2/gm 350-550
Moisture Content % <5.00

9. SILICA GEL 100-200 MESH
Related Categories: Flash
Chromatography, TLC
Grade: Laboratory Grade
Particle Size: 100-200 Mesh (149-
74μm))
Boiling Point: 2230 0C
Melting Point: >1600 0C
Bulk Density KG/LIT 500-650
Ph 10% aq solution 7 ± 0.5
Fe (Iron) <75 ppm
Chloride(NaCl) Max 300ppm
Pore Diameter –A 40-60A
Surface Area m2/gm 350-550
Moisture Content % <6.00

10. SILICA GEL 200-400 MESH
Related Categories: Flash
Chromatography,
TLC
Grade: Laboratory Grade
Particle Size: 200-400 Mesh (74-
37μm)
Boiling Point: 2230 0C
Melting Point: >1600 0C
Bulk Density KG/LIT 500-650
Ph 10% aq solution 7 ± 0.5
Fe (Iron) <65 ppm
Chloride(NaCl) Max 300ppm
Pore Diameter –A 40-60A
Surface Area m2/gm 450-650
Moisture Content % <5.00

11. SILICA GEL 230-400 MESH
Related Categories: Flash Chromatography,
TLC
Grade: Laboratory Grade
Particle Size: 230-400 Mesh (63-
37μm)
Boiling Point: 2230 0C
Melting Point: >1600 0C
Bulk Density KG/LIT 400-650
Ph 10% aq solution 7 ± 0.5
Fe (Iron) <60 ppm
Chloride(NaCl) Max 300ppm
Pore Diameter –A 40-60A
Surface Area m2/gm 450-650
Moisture Content % <5.00

12. SILICA GEL 400-800 MESH
Related Categories: Flash Chromatography,
TLC
Grade: Laboratory Grade
Particle Size: 400-800 Mesh (37-
18μm)
Boiling Point: 2230 0C
Melting Point: >1600 0C
Bulk Density KG/LIT 400-650
Ph 10% aq solution 7 ± 0.5
Fe (Iron) <60 ppm
Chloride(NaCl) Max 300ppm
Pore Diameter –A 55-60A
Surface Area m2/gm 450-650
Moisture Content % <5.00

13. Reference Link
• http://column-chromatography.com/chromatography-adsorbents/
• http://column-chromatography.com/thin-layer-chromatography-2/
• http://column-chromatography.com/silica-gel-column-chromatography/
• http://column-chromatography.com/silica-gel-100-200-mesh/
• http://column-chromatography.com/silica-gel-230-400-mesh/
• http://column-chromatography.com/aluminium-oxide-neutral/
• http://column-chromatography.com/aluminium-oxide-basic/
• http://column-chromatography.com/flash-chromatography-3/
• http://column-chromatography.com/blog/
• http://column-chromatography.com/silica-gel-gf/
• http://column-chromatography.com/silica-gel-60-120-mesh/
• http://column-chromatography.com/silica-gel-powder/
• http://column-chromatography.com/aluminium-oxide-chromatography/
• http://column-chromatography.com/extraction-of-bio-active-compounds-from-medicinal-plants/

14. For More Information
Contact Us
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Phone : +91-265-2761041
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Website: http://column-chromatography.com/
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