The disruption to trade caused by quarantine pests and diseases is a significant issue for Australian and New Zealand horticulture. There is an urgent need to review and improve the current disinfestation methods to develop new export protocols that are effective, economic and safe to use.
The project team consisting of researchers from across Australia and New Zealand, has reviewed all the published and unpublished phytosanitary research conducted against these pests.
Research presented in this session will explore some of our innovative research to improve pest management and help maintain and build market access for our grains industries.
This multifaceted project is seeking to protect the grains industry by improving the robustness and efficiency of the pest management and plant biosecurity system.
Planning and decision making to manage plant biosecurity risks is inherently complex, often contentious, involves unknowns and uncertainty, and needs to be adaptable to rapidly changing situations. The aim of this project is to develop a collaborative planning and shared decision making
framework that will result in better and faster decisions to respond more quickly to plant biosecurity risks, resulting in reduced impacts and costs, and more equitable and favourable outcomes for stakeholders and affected parties.
The disruption to trade caused by quarantine pests and diseases is a significant issue for Australian and New Zealand horticulture. There is an urgent need to review and improve the current disinfestation methods to develop new export protocols that are effective, economic and safe to use.
The project team consisting of researchers from across Australia and New Zealand, has reviewed all the published and unpublished phytosanitary research conducted against these pests.
Research presented in this session will explore some of our innovative research to improve pest management and help maintain and build market access for our grains industries.
This multifaceted project is seeking to protect the grains industry by improving the robustness and efficiency of the pest management and plant biosecurity system.
Planning and decision making to manage plant biosecurity risks is inherently complex, often contentious, involves unknowns and uncertainty, and needs to be adaptable to rapidly changing situations. The aim of this project is to develop a collaborative planning and shared decision making
framework that will result in better and faster decisions to respond more quickly to plant biosecurity risks, resulting in reduced impacts and costs, and more equitable and favourable outcomes for stakeholders and affected parties.
Here we update on fundamental systematics research and the development of new potential molecular markers to improve on current diagnostic tools. We also link these molecular tools with physical specimens, documenting the range of morphological variation so as to greatly improve on available resources used to diagnose fruit flies in the field as part of surveillance programmes or at border interceptions.
Invasive pests and pathogens can have devastating and unpredicted impacts on native ecosystems. The threat that Puccinia psidii (myrtle/eucalyptus/guava rust) posed to Australian industries was well recognised, but until its introduction in 2010, there was scant consideration of the impacts this disease may have on endemic Myrtaceous plant species and associated communities in native environments. Since its detection in Australia, the distribution and host range of P. psidii has rapidly expanded and entire species and plant communities are now under threat.
The research being undertaken into myrtle rust has enabled improved species selection for production and retail nurseries, and for urban tree planting.
Three years of research to date have produced a robust, accurate, sensitive detection tool and sampling strategy for the damaging apid-like insect phylloxera (Daktulosphaira vitifoliae), which feeds on grapevine roots
The Australia-Africa Plant Biosecurity Partnership has brought together plant biosecurity professionals in ten African countries and established linkages with Australian researchers, helping
to reduce pest and disease impacts in sub-Saharan Africa. At the outset of this initiative, diagnostic skills were identified as a priority area in connecting Australian expertise with Africa and improving surveillance capability, post-entry quarantine, early warning and phytosanitary certification. This presentation will briefly examine the application of improved diagnostic skills in African Plant Protection Organisations and the longer term relationships that have been established with Australian mentors.
Long distance natural (wind-assisted) dispersal of exotic plant pests and pathogens into Australia, is a very real and underestimated, biosecurity risk.
This research project is collecting data on past pest invasions in both Australia and New Zealand, in order to identify common patterns in plant biosecurity pests.
Here we update on fundamental systematics research and the development of new potential molecular markers to improve on current diagnostic tools. We also link these molecular tools with physical specimens, documenting the range of morphological variation so as to greatly improve on available resources used to diagnose fruit flies in the field as part of surveillance programmes or at border interceptions.
Invasive pests and pathogens can have devastating and unpredicted impacts on native ecosystems. The threat that Puccinia psidii (myrtle/eucalyptus/guava rust) posed to Australian industries was well recognised, but until its introduction in 2010, there was scant consideration of the impacts this disease may have on endemic Myrtaceous plant species and associated communities in native environments. Since its detection in Australia, the distribution and host range of P. psidii has rapidly expanded and entire species and plant communities are now under threat.
The research being undertaken into myrtle rust has enabled improved species selection for production and retail nurseries, and for urban tree planting.
Three years of research to date have produced a robust, accurate, sensitive detection tool and sampling strategy for the damaging apid-like insect phylloxera (Daktulosphaira vitifoliae), which feeds on grapevine roots
The Australia-Africa Plant Biosecurity Partnership has brought together plant biosecurity professionals in ten African countries and established linkages with Australian researchers, helping
to reduce pest and disease impacts in sub-Saharan Africa. At the outset of this initiative, diagnostic skills were identified as a priority area in connecting Australian expertise with Africa and improving surveillance capability, post-entry quarantine, early warning and phytosanitary certification. This presentation will briefly examine the application of improved diagnostic skills in African Plant Protection Organisations and the longer term relationships that have been established with Australian mentors.
Long distance natural (wind-assisted) dispersal of exotic plant pests and pathogens into Australia, is a very real and underestimated, biosecurity risk.
This research project is collecting data on past pest invasions in both Australia and New Zealand, in order to identify common patterns in plant biosecurity pests.
Food Safety – Mycotoxins, including aflatoxin – challenges for research and i...Francois Stepman
EU – African Collaboration on Food Systems for Nutrition:
24th January 2017
Food Safety – Mycotoxins, including aflatoxin – challenges for research and innovation - Approaches to Innovation
By
Andrew Emmott
PAEPARD,
Aflatoxin Expert Group
This is an assignment on the topic of "General Environment of Nestle & Unilever Pakistan" on the subject of Principles Of Management. Hope you guys will find it informative and helpful.
-Thank you
Invenire lead a project to establish a business ecosystem with edible insects in Finland, funded by Tekes, the Finnish Funding Agency for Innovation through its BioNets programme. The project ran from April to October 2016.
This Report summates our key findings on the state of the market, how an edible insects business ecosystem could be structured and function, and strategies to overcome existing roadblocks. This report also outlines key activities & outputs created through the project.
Unacceptable practises in industrial food production systems: Close confinement of pigs and poultry in cages; Barren environments with no enrichment; Routine mutilations; and Use of antibiotics for growth promotion and cover-up cruelty
A presentation on the Uppsala biotech cluster and its cluster organization - Uppsala BIO. In this presentation, Uppsala BIO is seen as an agent of change in terms of its strategy and efforts to improve the competitiveness of the Uppsala biotech cluster.
Attend a Free Public Evening Lecture at SCI to hear why we there is a need for analysis based on good science that balances both the needs of UK farmers and the needs of the wildlife that uses their crops as habitat.
Research investigating the use a genome-informed approach to develop diagnostic tools, for the detection of exotic phytopathogenic bacteria that pose a threat to Australian agriculture.
The aim of this research project is to establish Australian developed seed testing protocols as an international standard for the detection of viroids and cucumber green mottle mosaic virus (CGMMV) in seed, and to reduce the risks of contaminated traded seed.
The diagnosis of viral pathogens is a crucial component of plant biosecurity surveillance and preventing the introduction of exotic plant viruses and viroids at the border. Existing quarantine procedures can be time-consuming and require detailed knowledge of potential infecting viral pathogens. Currently, imported plants can spend as long as two years in quarantine, with associated costs.
To simplify the post-entry quarantine process researchers have developed a plant diagnostic toolkit for plant viruses and viroids. The toolkit takes advantage of the natural antiviral system of plants, using small RNA next generation sequencing (sRNA-seq) technology to detect nearly all known viruses and viroids in a single test. The new test, and associated toolkit, will reduce the time imported plant material spends in Australia’s quarantine system while improving accuracy of detection in a single sRNA-seq experiment.
This research has developed recommendations for stakeholders involved in area-wide management of fruit fly, including social and institutional requirements.
This project aims to build the ability of indigenous communities (Maori and Aboriginal), regulatory authorities and industries to better manage the impact of biosecurity threats. Models have been developed for Indigenous engagement.
This social biosecurity project, aims to improve plant biosecurity management by developing the capacity of regional and remote communities to engage in biosecurity surveillance activities.
Surveillance systems are an essential component of biosecurity. Design of biosecurity surveillance systems may include designs of grids of static traps, plans for field sampling, or deployment of potentially "game-changing" mobile trap technology. The aim of these systems is to achieve defined detection objectives, (e.g. early detection, supporting area-freedom status) at minimum cost. This project will develop and apply statistically-based surveillance systems that account for organism biology, trap behaviour and landscape characteristics.
Ships arriving in Australia may have visited multiple ports along the way. These complex pathways present opportunities for pest species, such as the Asian Gypsy Moth, to arrive into Australia from indirect routes. Understanding those pathways that link Australia directly or indirectly to countries in which a pest or disease occurs is necessary to identify arriving ships with the highest likelihood of carrying hitchhiker species. This project proposes to address three important questions:
1. What general shipping pathways pose the greatest risk?
2. How to make decisions regarding what ships to search?
3. How much inspection to conduct?
This research will investigate technologies to enable the development of spore traps capable of in-field detection, and identification, of specific biosecurity threats.
The spread of invasive species continues to provide significant challenges to those government biosecurity agencies charged with protecting a country’s borders. In an increasingly connected world, these invasive species are potentially able to spread further and more rapidly. Human mediated pathways such as ships and airlines are the most obvious ways in which invasive species can be spread. Direct routes from one port to another are currently monitored, but indirect pathways,
in which a ship picks up an invasive species and then travels to a number of different locations before arriving at the final destination, present more challenging scenarios. For the Australian Government Department of Agriculture, one particular concern is for ships arriving into Australia carrying viable eggs of the Asian gypsy moth (Lymantria dispar). We are developing a real time tool that will analyse the pathways for incoming ships and determine the likelihood the ship could be carrying viable eggs.
Biosecurity issues impact on key crops and environmental values across NZ and Australia. A key outcome for the project team will be the ability of indigenous communities, and relevant regulatory authorities and industries, to better manage the social, environmental and economic impacts of biosecurity threats, and to participate in biosecurity strategies through improved bicultural engagement models that build empowerment and ownership in indigenous communities and their response to those threats. The teams have developed an engagement model adapted to the indigenous peoples and their communities of each country.
The results of a baseline study on motivation and incentives involved in the decisions to control fruit fly highlight the variability of motivations within demographic groups.
Myrtle rust (Puccinia psidii) is an invasive fungus native to South America that was first detected in Australia in 2010. It has spread rapidly along the east coast, and is currently recorded in sites as far north as the Northern Territory and south to Tasmania.
In order to better understand myrtle rust extent and impacts in Australian native and managed landscapes, a survey was sent to national parks, botanical gardens, councils, natural resource
managers, nurseries and forestry agencies in all states where the fungus is present (NT, QLD, NSW, VIC and TAS). The survey revealed that Myrtle rust is widespread in NSW and QLD gardens and streets as well as in native vegetation.
The risk myrtle rust poses to threatened Myrtaceae species in Australia is becoming more apparent with significant dieback and tree death recorded as a result of repeated infection.
Research presented in this session addresses the need to better understand the impacts of pests and diseases on the environment and the various ways that social science and the rules of community engagement can be applied for better biosecurity.
Chlorine dioxide was tested in three pilot scale structures including the mini silo, the column, and the flat box against five stored-product insect pests, including adults of Rhyzopertha dominica (F.), Tribolium castaneum (Herbst), Oryzaephilus surinamensis (L.), Sitophilus zeamais Motschulsky, and
Sitophilus oryzae (L.).
The Global Eradication and Response Database (GERDA) summarises incursion response and eradication programmes from around the world. The purpose of GERDA is to determine which characteristics lead to the success or failure of eradication programmes.
Bacterial leaf spot (BLS) is a disease of tomato, chilli and capsicum caused by four species of Xanthomonas. BLS can cause severe crop damage and is a limiting factor of production across the world.
More from Plant Biosecurity Cooperative Research Centre (20)
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Session 4: Amorphous silica – a commercial path to impact
1. Amorphous silica – a commercial path to impact
David Eagling -
on behalf of the 40+ contributors
Plant Biosecurity Cooperative Research Centre
2. biosecurity built on science
What would it mean if you could control insects without chemicals?
Food security - FAO: “given many smallholder
farmers live on the margins of food insecurity, a
reduction in food losses could have an immediate
and significant impact on their livelihoods”
Global consumers
- Strong market demand for non-
chemical options e.g. organics
- Non-regulatory supermarket drivers
e.g. EU is decreasing residue
tolerances for chemicals
Biosecurity - Insects affect grain quality and Australia trades grain on the concept of
“nil tolerance for live insects”
3. biosecurity built on science
Research – We have been “rediscovering” silica
Red flour beetle
Tribolium castaneum
Lesser grain borer
Rhyzopertha dominica
Rice weevil
Sitophilus oryzae
5 days
exposure
10 days
exposure
5 days
exposure
10 days
exposure
5 days
exposure
10 days
exposure
Silica No.1 100 100 99 100 100 100
Silica No. 2 99 100 99 100 100 100
Silica No. 3 100 100 100 100 100 100
Dryacide 0 5 40 75 49 79
Absorbacide 1 7 36 68 51 78
Diafil 610 3 7 42 74 53 70
Percentage mortality of various grain storage pests to both “new” amorphous silica
products and existing commercial silica products
4. biosecurity built on science
Invention – How to optimise and deliver an amorphous silica product
Image copyright: Fumed silica process.svg
5. biosecurity built on science
PBCRC / PBIP agree – impact requires a commercial path to market
Impact - Small business and start-ups are
important to the economy:
The majority of businesses in Australia are
small - accounting for 97 percent of all
businesses
More than 60 percent of small businesses
cease operating within the first three years
of starting
Success can be profound:
- Glaxo (worlds 6th largest pharmaceutical company) was founded in the 1850s in rural
New Zealand as a general trading company
Australia and innovation – The “top 5” countries for supporting business start-ups are
(1) Singapore (2) HK (3) China (4) NZ (5) US - But where is Australia?
6. biosecurity built on science
Innovation - We continue to move forward to market
1. Research and Invention - navigating both scientific and commercial literature
5. People and Structures – the PBCRC provides in-principle support for
a “spin-out”
3. Innovation - building a business plan and
explaining how everyone will make money
“The value of an idea lies in the using of it” —Thomas Edison
4. Markets – opportunities
everywhere but the business plan
provides the required focus
2. To get to market we require product registration