This document discusses the use of chitosan for managing post-harvest diseases of fruits and vegetables. It provides background on chitosan, including its extraction from crustacean shells, properties, and commercial products. The document examines chitosan's effectiveness against bacterial and fungal diseases through mechanisms like disrupting microbial membranes. Factors like pH, concentration, and molecular weight are found to influence chitosan's antimicrobial activity. Studies demonstrate chitosan's ability to inhibit the growth of fungal pathogens like Colletotrichum and reduce decay in fruits. Overall, the document reviews the role of chitosan in post-harvest disease management.
Chitosan as a potential natural compound to controlPuja41124
The document discusses the potential of chitosan as a natural compound to manage post-harvest diseases of horticultural crops. It provides background on chitosan including its definition, production, and mechanism of action in activating plant defense systems. Studies show chitosan reduces post-harvest diseases in various crops like tomatoes and strawberries by inhibiting fungal growth, maintaining cell wall integrity, and eliciting defensive enzyme activities and phenolic compounds. Chitosan coating combined with calcium treatment helps preserve quality in strawberries during refrigerated storage.
Exploitation of endophytic fungi for plant disease management
Introduction
Plant- Endophytic fungi interaction
Diversity of endophytic fungi in plants
Colonization
Endophytic fungi : Mechanism
Case studies
Conclusion
Future aspects
Endophytic fungi in disease resistance (Latz et al., 2018)
Antibiotics produced by fungal endophytes
Plant immune defense system
Lytic enzyme secretion
Endophytic fungi in stress tolerance
Mechanism of disease control by endophytesPooja Bhatt
The document discusses alternative methods for pest management to address problems with chemical pesticides such as development of resistance and environmental contamination. It suggests that biological control using endophytic microorganisms is a promising alternative as endophytes have antagonistic properties against plant pathogens. Endophytes can inhibit pathogens through direct mechanisms such as hyperparasitism, competition, antibiosis, and lytic enzyme production or indirect induction of host plant resistance. Case studies provide examples of endophytes inhibiting fungal plant pathogens through siderophore production, parasitic growth, and antibiotic compounds.
phyllosphere is a dynamic rapidly changing area surrounding the germinating seed. there are two categories of microbes one is positively enhancing and negatively reducing the plant yield
This document discusses wheat blast, a disease of wheat caused by the fungus Magnaporthe oryzae Triticum pathotype. It was first observed in Brazil in 1985 and has since spread to other parts of South America, Africa, and Asia. In Bangladesh, it was first spotted in 2016 and spread to about 15,000 hectares. Symptoms include spots on leaves and blackened spikes. Control methods include using resistant varieties, crop rotation, removing debris, and applying fungicides. Ongoing monitoring is needed to prevent further spread.
This document discusses plant growth promoting rhizobacteria (PGPR). It begins by noting the growing global population and need to increase food production. It then defines PGPR as bacteria that colonize plant roots and promote growth through various mechanisms. The document goes on to describe characteristics, mechanisms, and examples of PGPR, including biological nitrogen fixation, phosphate solubilization, phytohormone production, siderophore production, induced systemic resistance, and stress tolerance functions. A history of PGPR research is also provided, along with commercial examples.
Endophytic microbes live within plant tissues without causing harm and can benefit plants through various mechanisms. This document discusses endophytic bacteria and fungi, their transmission within plants, and how they can promote plant growth, act as biocontrol agents, and increase stress tolerance in plants. Specifically, endophytes produce plant hormones, fix nitrogen, make nutrients more available, and induce systemic resistance to pathogens or tolerance to stresses like drought. Their interactions with plants demonstrate potential for agriculture and phytoremediation.
This document summarizes induced plant resistance against pathogens. It discusses the historical background of induced resistance being first observed over 100 years ago. It describes different types of induced resistance including systemic acquired resistance (SAR) and induced systemic resistance (ISR). SAR is mediated by salicylic acid and involves pathogenesis-related proteins, while ISR is mediated by jasmonic acid and ethylene. Biological agents like PGPR bacteria and plant extracts can also induce resistance. Signal transduction pathways underlying these responses are triggered upon pathogen recognition. While induced resistance offers opportunities for crop protection, practical applications are currently limited to some plants.
Chitosan as a potential natural compound to controlPuja41124
The document discusses the potential of chitosan as a natural compound to manage post-harvest diseases of horticultural crops. It provides background on chitosan including its definition, production, and mechanism of action in activating plant defense systems. Studies show chitosan reduces post-harvest diseases in various crops like tomatoes and strawberries by inhibiting fungal growth, maintaining cell wall integrity, and eliciting defensive enzyme activities and phenolic compounds. Chitosan coating combined with calcium treatment helps preserve quality in strawberries during refrigerated storage.
Exploitation of endophytic fungi for plant disease management
Introduction
Plant- Endophytic fungi interaction
Diversity of endophytic fungi in plants
Colonization
Endophytic fungi : Mechanism
Case studies
Conclusion
Future aspects
Endophytic fungi in disease resistance (Latz et al., 2018)
Antibiotics produced by fungal endophytes
Plant immune defense system
Lytic enzyme secretion
Endophytic fungi in stress tolerance
Mechanism of disease control by endophytesPooja Bhatt
The document discusses alternative methods for pest management to address problems with chemical pesticides such as development of resistance and environmental contamination. It suggests that biological control using endophytic microorganisms is a promising alternative as endophytes have antagonistic properties against plant pathogens. Endophytes can inhibit pathogens through direct mechanisms such as hyperparasitism, competition, antibiosis, and lytic enzyme production or indirect induction of host plant resistance. Case studies provide examples of endophytes inhibiting fungal plant pathogens through siderophore production, parasitic growth, and antibiotic compounds.
phyllosphere is a dynamic rapidly changing area surrounding the germinating seed. there are two categories of microbes one is positively enhancing and negatively reducing the plant yield
This document discusses wheat blast, a disease of wheat caused by the fungus Magnaporthe oryzae Triticum pathotype. It was first observed in Brazil in 1985 and has since spread to other parts of South America, Africa, and Asia. In Bangladesh, it was first spotted in 2016 and spread to about 15,000 hectares. Symptoms include spots on leaves and blackened spikes. Control methods include using resistant varieties, crop rotation, removing debris, and applying fungicides. Ongoing monitoring is needed to prevent further spread.
This document discusses plant growth promoting rhizobacteria (PGPR). It begins by noting the growing global population and need to increase food production. It then defines PGPR as bacteria that colonize plant roots and promote growth through various mechanisms. The document goes on to describe characteristics, mechanisms, and examples of PGPR, including biological nitrogen fixation, phosphate solubilization, phytohormone production, siderophore production, induced systemic resistance, and stress tolerance functions. A history of PGPR research is also provided, along with commercial examples.
Endophytic microbes live within plant tissues without causing harm and can benefit plants through various mechanisms. This document discusses endophytic bacteria and fungi, their transmission within plants, and how they can promote plant growth, act as biocontrol agents, and increase stress tolerance in plants. Specifically, endophytes produce plant hormones, fix nitrogen, make nutrients more available, and induce systemic resistance to pathogens or tolerance to stresses like drought. Their interactions with plants demonstrate potential for agriculture and phytoremediation.
This document summarizes induced plant resistance against pathogens. It discusses the historical background of induced resistance being first observed over 100 years ago. It describes different types of induced resistance including systemic acquired resistance (SAR) and induced systemic resistance (ISR). SAR is mediated by salicylic acid and involves pathogenesis-related proteins, while ISR is mediated by jasmonic acid and ethylene. Biological agents like PGPR bacteria and plant extracts can also induce resistance. Signal transduction pathways underlying these responses are triggered upon pathogen recognition. While induced resistance offers opportunities for crop protection, practical applications are currently limited to some plants.
Wheat blast is an emerging fungal disease caused by Magnaporthe oryzae that poses a serious threat to global wheat production. It was first discovered in Brazil in 1985 and has since spread to other parts of South America. In 2016, it was observed for the first time in Bangladesh. Wheat is a highly important food crop worldwide, providing 20% of global calorie intake. Effective management of wheat blast is challenging due to limited resistance genes identified so far and the unreliable control by fungicides. Host resistance and surveillance are currently the most effective control strategies to prevent further global spread of this disease.
Eco friendly management of fungal seed borne pathogens through bio-agentsAnkit Chaudhari
Seed borne diseases causes heavy losses in the crops at all stages of growth like seed germination, seedling and maturity of plants.
Bio-control technologies have gained momentum in disease control of crop plants, in recent times as these technologies not only minimize or replace the usage of harmful chemical pesticides, but also found to be ecofriendly, environmentally safe, cheaper and efficient in certain disease control programmes.
Fungal bio-control agents like Trichoderma spp. successfully used for the control of many seed borne diseases caused by Aspergillus spp., Alternaria spp., Curvularia spp., Colletotrichum spp., Fusarium spp., Pyricularia spp., Helminthosporium spp. etc. in several crops.
Abiotic Stress in Mushroom Production
Besides the biotic agents, which adversely affect the mushrooms, there are a large number of abiotic agents which create unfavourable environment for the proper growth of 170 Mushrooms: Cultivation, Marketing and Consumption mushrooms resulting in the quantitative as well as qualitative losses. These abiotic agents include temperature, relative humidity, low or high moisture in the substrate, pH, CO2 concentration in the room, wind velocity and fumes. Some of the most common abnormalities generally encountered during button mushroom cultivation are as under.
1. The document summarizes a seminar presentation on recent advances in biological management of rice diseases.
2. It describes several major fungal, bacterial, viral and nematode diseases that affect rice, including blast, brown spot, bacterial blight, false smut, sheath rot, sheath blight, tungro disease, and nematode diseases.
3. It then discusses biological control as an alternative to chemical pesticides for managing rice diseases, highlighting various fungi like Trichoderma species and bacteria like Pseudomonas and Bacillus as potential biological control agents.
This document summarizes herbicide resistance in weeds. It discusses the history and mechanisms of herbicide resistance, including target-site mutations and metabolism-based resistance. The modes and sites of action of herbicides are explained. Herbicide resistance develops through repeated herbicide use selecting for naturally resistant weed populations. Integrated weed management is important to manage resistance, including herbicide mixtures and rotations, proper application, and preventing weed seed production.
Use of Pseudomonas fluoroscens as biocontrol agentSandeep Kumar
This document discusses the use of Pseudomonas fluorescens as a biocontrol agent. P. fluorescens is a common soil bacterium that acts as a biological control agent against various plant pathogens through multiple modes of action, including antibiotic production, siderophore production, induced systemic resistance, competition, and hydrogen cyanide production. It can also promote plant growth. The document provides details on the isolation, multiplication, and use of P. fluorescens as a biocontrol agent.
Role of Silicon in Alleviating Biotic and Abiotic Stresses in PlantsBHU,Varanasi, INDIA
1) Silicon plays an important role in alleviating biotic and abiotic stresses in plants. It provides strength to cell walls and improves growth, health, and productivity.
2) Silicon is taken up by plants through monosilicic acid and polysilicic acid in the soil. It accumulates in leaves and other organs. Gene research has identified silicon transporters that allow for uptake in rice.
3) Applying silicon enhances plant resistance to diseases and pests. It acts as a physical barrier on plant surfaces and boosts the plant's defenses. Silicon application increases crop yields and quality.
Microbial endophytes are microorganisms that live within plant tissues without causing disease or harm. They have been isolated from many plant species worldwide and show diversity across environments. Endophytes are isolated from surface-sterilized plant tissues and identified based on genetic sequencing. Bacterial endophytes provide benefits like nitrogen fixation, plant growth promotion, biocontrol of pathogens, and abiotic stress resistance. Fungal endophytes also produce secondary metabolites and biocontrol pathogens. Future work aims to develop endophytes as biofertilizers and biopesticides through improved isolation techniques and delivery methods.
This document provides information on several entomopathogenic fungi used for microbial control of insect pests. It discusses the fungi Beauveria bassiana, Metarhizium anisopliae, Verticillium lecanii, Paecilomyces fumosoroseus, and Hirsutella thomsoni. For each fungus, it describes the target pests, mass production methods, and field application procedures. The document emphasizes that these entomopathogenic fungi are effective alternatives to chemical pesticides for controlling agricultural insect pests while causing less damage to the environment.
Potentialities and Constraints of Liquid Biofertilizers.pptxUAS, Dharwad
This document discusses the potential and constraints of liquid biofertilizers. It begins with an introduction on the importance of exploring biofertilizers as a more sustainable alternative to chemical fertilizers. It then discusses different types of biofertilizers, highlighting the advantages of liquid biofertilizers over carrier-based formulations in terms of longer shelf life and higher viability. The document reviews research showing positive effects of liquid biofertilizers on crop germination, growth, and yield. It also discusses constraints of liquid biofertilizers and concludes with potential future research directions.
Biochar and its use in plant disease managementBISWAJITJENA64
This document discusses biochar and its potential use in plant disease management. It provides background on biochar production through pyrolysis and discusses several proposed mechanisms by which biochar may suppress plant diseases. These include inducing systemic resistance in plants, enhancing beneficial microbes, modifying soil quality, direct fungitoxic effects, and sorption of allelopathic compounds. The document reviews experimental evidence supporting these mechanisms from studies on various plant pathogens. It also summarizes research on biochar sorption of agrochemicals and phytotoxic compounds. Overall, the document examines the promising role of biochar as a tool for controlling plant diseases through multiple proposed mechanisms.
This document summarizes a seminar on breeding crops for resistance to biotic and abiotic stresses. It discusses how abiotic stresses like high/low temperatures, drought, salinity, and toxicity reduce crop yields worldwide by 65-87% on average. It describes how stresses cause oxidative stress in plants by producing reactive oxygen species. It then outlines various abiotic stress factors and how they impact plants, as well as stress tolerance mechanisms employed by plants, such as osmoprotectants and heat avoidance through transpirational cooling or leaf rolling. Finally, it provides examples of crop varieties that are tolerant to high temperatures, humidity, drought, and other stresses.
The document discusses the development of Phytophthora and Pythium databases to support the identification and monitoring of these major plant pathogen groups. It describes the objectives of building a cyberinfrastructure to archive genotype, phenotype and distribution data on Phytophthora species/isolates. The Phytophthora Database provides tools for sequence analysis, phylogenetic analysis and molecular identification. Future directions include expanding to other plant pathogen databases and integrating genomic and geospatial data.
major as well as minor viral disease of cereals, legumes, oil-crops, vegetables has been listed. The major research works done these diseases has been also included along with first report.
The document discusses plant growth promoting rhizobacteria (PGPR) and their mechanisms and functions in promoting plant growth. It describes how PGPR can directly promote plant growth through mechanisms like nitrogen fixation, phosphate solubilization, siderophore production and phytohormone production. PGPR also indirectly promote growth by inhibiting pathogens through producing antibiotics, lytic enzymes and inducing systemic resistance in plants. Future research areas discussed include developing PGPR consortium, improving stress tolerance and making PGPR products more cost effective and environmentally friendly.
This document discusses methods for managing plant diseases, including fungicides. It covers cultural practices like removing debris, improving growing conditions and using crop rotation. It describes chemical controls like fungicides and their classification. It also discusses breeding resistant varieties, biological controls using beneficial microbes, and quarantine. The last method is doing nothing and accepting some disease impact.
The concept of gene for gene hypothesis was first developed by Flor in 1956 based on his studies of host pathogen interaction in flax, for rust caused by Melampsora lini. The gene for gene hypothesis states that for each gene controlling resistance in the host, there is corresponding gene controlling pathogenicity in the pathogen. The resistance of host is governed by dominant genes and virulence of pathogen by recessive genes. The genotype of host and pathogen determine the disease reaction. When genes in host and pathogen match for all loci, then only the host will show susceptible reaction. If some gene loci remain unmatched, the host will show resistant reaction. Now gene – for –gene relationship has been reported in several other crops like potato, sorghum, wheat, etc. The gene for gene hypothesis is also known as “Flor Hypothesis.”
Silica nanoparticles show potential as a new insecticide for pest control. Researchers found the first record of using nanotechnology in agriculture by testing silica nanoparticles on insects. The nanoparticles proved effective at killing insects, demonstrating their potential as an environmentally-friendly alternative to traditional chemical pesticides.
This document discusses methods for identifying plant pathogens. Traditional visual examination can only identify damage after it has already occurred. More sensitive early diagnosis methods are needed to treat pathogens before irreparable damage. Modern methods like polymerase chain reaction (PCR) and serological techniques can identify pathogens before visible symptoms appear, allowing treatment before significant yield losses. These methods help identify the causal agent through DNA analysis and other laboratory techniques.
This slide will help in understanding the symptoms, effects of bacterial wilt on solencous crops like Potato, Brinjil and Pepper.I hope all of you will understand my points.
The study was carried out with the aim of sourcing for bacteria from the natural environment having antifungal capabilities to control and inhibit postharvest fungal spoilage of fruits and vegetables caused by Botrytis cinerea. Soil and water samples were collected from Heriot Watt University environment and Dr Ruth Fowler’s garden and inoculated using the spread plate technique; identification was carried out using Microbact Identification kits; and isolates assayed for antifungal activities against Botrytis cinerea. Forty eight bacteria species were isolated out of which sixteen (16) belonging to genera Pseudomonas, Bacillus, Escherichia, Burkholderia, Staphylococcus, Streptococcus, and Proteus showed antifungal activities. Bacteria species Pseudomonas stutzeri and Burkholderia cepacia had the highest zones of inhibition with average radii of 3.06 and 3.20 cm respectively. The bacteria had the potential to inhibit mycelial and spore growth at varying levels thus making them possible candidates for further tests and studies. Considering the aim of the study, further research into identifying these antifungal isolates inhibitory compounds and metabolites is highly recommended.
Engineering pathogen resistance in crop plants current trends and future pros...UdayenduL10
This document summarizes engineering pathogen resistance in crop plants through genetic engineering techniques. It discusses current trends in developing disease resistance including directly interfering with pathogen virulence factors, regulating natural host defense responses, and pathogen mimicry. Transgenic crops with resistance to fungi, viruses and bacteria are still a small proportion of commercialized GM crops which are dominated by herbicide and insect resistance traits. Future work needs a better understanding of plant-pathogen interactions and developing durable resistance strategies. While transgenic resistance has been successful for viruses in papaya, socioeconomic acceptance of GM crops remains a challenge.
Wheat blast is an emerging fungal disease caused by Magnaporthe oryzae that poses a serious threat to global wheat production. It was first discovered in Brazil in 1985 and has since spread to other parts of South America. In 2016, it was observed for the first time in Bangladesh. Wheat is a highly important food crop worldwide, providing 20% of global calorie intake. Effective management of wheat blast is challenging due to limited resistance genes identified so far and the unreliable control by fungicides. Host resistance and surveillance are currently the most effective control strategies to prevent further global spread of this disease.
Eco friendly management of fungal seed borne pathogens through bio-agentsAnkit Chaudhari
Seed borne diseases causes heavy losses in the crops at all stages of growth like seed germination, seedling and maturity of plants.
Bio-control technologies have gained momentum in disease control of crop plants, in recent times as these technologies not only minimize or replace the usage of harmful chemical pesticides, but also found to be ecofriendly, environmentally safe, cheaper and efficient in certain disease control programmes.
Fungal bio-control agents like Trichoderma spp. successfully used for the control of many seed borne diseases caused by Aspergillus spp., Alternaria spp., Curvularia spp., Colletotrichum spp., Fusarium spp., Pyricularia spp., Helminthosporium spp. etc. in several crops.
Abiotic Stress in Mushroom Production
Besides the biotic agents, which adversely affect the mushrooms, there are a large number of abiotic agents which create unfavourable environment for the proper growth of 170 Mushrooms: Cultivation, Marketing and Consumption mushrooms resulting in the quantitative as well as qualitative losses. These abiotic agents include temperature, relative humidity, low or high moisture in the substrate, pH, CO2 concentration in the room, wind velocity and fumes. Some of the most common abnormalities generally encountered during button mushroom cultivation are as under.
1. The document summarizes a seminar presentation on recent advances in biological management of rice diseases.
2. It describes several major fungal, bacterial, viral and nematode diseases that affect rice, including blast, brown spot, bacterial blight, false smut, sheath rot, sheath blight, tungro disease, and nematode diseases.
3. It then discusses biological control as an alternative to chemical pesticides for managing rice diseases, highlighting various fungi like Trichoderma species and bacteria like Pseudomonas and Bacillus as potential biological control agents.
This document summarizes herbicide resistance in weeds. It discusses the history and mechanisms of herbicide resistance, including target-site mutations and metabolism-based resistance. The modes and sites of action of herbicides are explained. Herbicide resistance develops through repeated herbicide use selecting for naturally resistant weed populations. Integrated weed management is important to manage resistance, including herbicide mixtures and rotations, proper application, and preventing weed seed production.
Use of Pseudomonas fluoroscens as biocontrol agentSandeep Kumar
This document discusses the use of Pseudomonas fluorescens as a biocontrol agent. P. fluorescens is a common soil bacterium that acts as a biological control agent against various plant pathogens through multiple modes of action, including antibiotic production, siderophore production, induced systemic resistance, competition, and hydrogen cyanide production. It can also promote plant growth. The document provides details on the isolation, multiplication, and use of P. fluorescens as a biocontrol agent.
Role of Silicon in Alleviating Biotic and Abiotic Stresses in PlantsBHU,Varanasi, INDIA
1) Silicon plays an important role in alleviating biotic and abiotic stresses in plants. It provides strength to cell walls and improves growth, health, and productivity.
2) Silicon is taken up by plants through monosilicic acid and polysilicic acid in the soil. It accumulates in leaves and other organs. Gene research has identified silicon transporters that allow for uptake in rice.
3) Applying silicon enhances plant resistance to diseases and pests. It acts as a physical barrier on plant surfaces and boosts the plant's defenses. Silicon application increases crop yields and quality.
Microbial endophytes are microorganisms that live within plant tissues without causing disease or harm. They have been isolated from many plant species worldwide and show diversity across environments. Endophytes are isolated from surface-sterilized plant tissues and identified based on genetic sequencing. Bacterial endophytes provide benefits like nitrogen fixation, plant growth promotion, biocontrol of pathogens, and abiotic stress resistance. Fungal endophytes also produce secondary metabolites and biocontrol pathogens. Future work aims to develop endophytes as biofertilizers and biopesticides through improved isolation techniques and delivery methods.
This document provides information on several entomopathogenic fungi used for microbial control of insect pests. It discusses the fungi Beauveria bassiana, Metarhizium anisopliae, Verticillium lecanii, Paecilomyces fumosoroseus, and Hirsutella thomsoni. For each fungus, it describes the target pests, mass production methods, and field application procedures. The document emphasizes that these entomopathogenic fungi are effective alternatives to chemical pesticides for controlling agricultural insect pests while causing less damage to the environment.
Potentialities and Constraints of Liquid Biofertilizers.pptxUAS, Dharwad
This document discusses the potential and constraints of liquid biofertilizers. It begins with an introduction on the importance of exploring biofertilizers as a more sustainable alternative to chemical fertilizers. It then discusses different types of biofertilizers, highlighting the advantages of liquid biofertilizers over carrier-based formulations in terms of longer shelf life and higher viability. The document reviews research showing positive effects of liquid biofertilizers on crop germination, growth, and yield. It also discusses constraints of liquid biofertilizers and concludes with potential future research directions.
Biochar and its use in plant disease managementBISWAJITJENA64
This document discusses biochar and its potential use in plant disease management. It provides background on biochar production through pyrolysis and discusses several proposed mechanisms by which biochar may suppress plant diseases. These include inducing systemic resistance in plants, enhancing beneficial microbes, modifying soil quality, direct fungitoxic effects, and sorption of allelopathic compounds. The document reviews experimental evidence supporting these mechanisms from studies on various plant pathogens. It also summarizes research on biochar sorption of agrochemicals and phytotoxic compounds. Overall, the document examines the promising role of biochar as a tool for controlling plant diseases through multiple proposed mechanisms.
This document summarizes a seminar on breeding crops for resistance to biotic and abiotic stresses. It discusses how abiotic stresses like high/low temperatures, drought, salinity, and toxicity reduce crop yields worldwide by 65-87% on average. It describes how stresses cause oxidative stress in plants by producing reactive oxygen species. It then outlines various abiotic stress factors and how they impact plants, as well as stress tolerance mechanisms employed by plants, such as osmoprotectants and heat avoidance through transpirational cooling or leaf rolling. Finally, it provides examples of crop varieties that are tolerant to high temperatures, humidity, drought, and other stresses.
The document discusses the development of Phytophthora and Pythium databases to support the identification and monitoring of these major plant pathogen groups. It describes the objectives of building a cyberinfrastructure to archive genotype, phenotype and distribution data on Phytophthora species/isolates. The Phytophthora Database provides tools for sequence analysis, phylogenetic analysis and molecular identification. Future directions include expanding to other plant pathogen databases and integrating genomic and geospatial data.
major as well as minor viral disease of cereals, legumes, oil-crops, vegetables has been listed. The major research works done these diseases has been also included along with first report.
The document discusses plant growth promoting rhizobacteria (PGPR) and their mechanisms and functions in promoting plant growth. It describes how PGPR can directly promote plant growth through mechanisms like nitrogen fixation, phosphate solubilization, siderophore production and phytohormone production. PGPR also indirectly promote growth by inhibiting pathogens through producing antibiotics, lytic enzymes and inducing systemic resistance in plants. Future research areas discussed include developing PGPR consortium, improving stress tolerance and making PGPR products more cost effective and environmentally friendly.
This document discusses methods for managing plant diseases, including fungicides. It covers cultural practices like removing debris, improving growing conditions and using crop rotation. It describes chemical controls like fungicides and their classification. It also discusses breeding resistant varieties, biological controls using beneficial microbes, and quarantine. The last method is doing nothing and accepting some disease impact.
The concept of gene for gene hypothesis was first developed by Flor in 1956 based on his studies of host pathogen interaction in flax, for rust caused by Melampsora lini. The gene for gene hypothesis states that for each gene controlling resistance in the host, there is corresponding gene controlling pathogenicity in the pathogen. The resistance of host is governed by dominant genes and virulence of pathogen by recessive genes. The genotype of host and pathogen determine the disease reaction. When genes in host and pathogen match for all loci, then only the host will show susceptible reaction. If some gene loci remain unmatched, the host will show resistant reaction. Now gene – for –gene relationship has been reported in several other crops like potato, sorghum, wheat, etc. The gene for gene hypothesis is also known as “Flor Hypothesis.”
Silica nanoparticles show potential as a new insecticide for pest control. Researchers found the first record of using nanotechnology in agriculture by testing silica nanoparticles on insects. The nanoparticles proved effective at killing insects, demonstrating their potential as an environmentally-friendly alternative to traditional chemical pesticides.
This document discusses methods for identifying plant pathogens. Traditional visual examination can only identify damage after it has already occurred. More sensitive early diagnosis methods are needed to treat pathogens before irreparable damage. Modern methods like polymerase chain reaction (PCR) and serological techniques can identify pathogens before visible symptoms appear, allowing treatment before significant yield losses. These methods help identify the causal agent through DNA analysis and other laboratory techniques.
This slide will help in understanding the symptoms, effects of bacterial wilt on solencous crops like Potato, Brinjil and Pepper.I hope all of you will understand my points.
The study was carried out with the aim of sourcing for bacteria from the natural environment having antifungal capabilities to control and inhibit postharvest fungal spoilage of fruits and vegetables caused by Botrytis cinerea. Soil and water samples were collected from Heriot Watt University environment and Dr Ruth Fowler’s garden and inoculated using the spread plate technique; identification was carried out using Microbact Identification kits; and isolates assayed for antifungal activities against Botrytis cinerea. Forty eight bacteria species were isolated out of which sixteen (16) belonging to genera Pseudomonas, Bacillus, Escherichia, Burkholderia, Staphylococcus, Streptococcus, and Proteus showed antifungal activities. Bacteria species Pseudomonas stutzeri and Burkholderia cepacia had the highest zones of inhibition with average radii of 3.06 and 3.20 cm respectively. The bacteria had the potential to inhibit mycelial and spore growth at varying levels thus making them possible candidates for further tests and studies. Considering the aim of the study, further research into identifying these antifungal isolates inhibitory compounds and metabolites is highly recommended.
Engineering pathogen resistance in crop plants current trends and future pros...UdayenduL10
This document summarizes engineering pathogen resistance in crop plants through genetic engineering techniques. It discusses current trends in developing disease resistance including directly interfering with pathogen virulence factors, regulating natural host defense responses, and pathogen mimicry. Transgenic crops with resistance to fungi, viruses and bacteria are still a small proportion of commercialized GM crops which are dominated by herbicide and insect resistance traits. Future work needs a better understanding of plant-pathogen interactions and developing durable resistance strategies. While transgenic resistance has been successful for viruses in papaya, socioeconomic acceptance of GM crops remains a challenge.
This review article summarizes biocontrol strategies for managing Colletotrichum species that cause anthracnose disease in postharvest fruits. It discusses strategies using bacteria, filamentous fungi, and yeasts to control three main Colletotrichum clades: C. acutatum, C. gloeosporioides, and C. truncatum. The most effective bacterial biocontrol agents for C. acutatum were Bacillus subtilis, Paenibacillus polymyxa, and Bacillus amyloliquefaciens. Filamentous fungi like Cryptococcus laurentii and Aureobasidium pullulans showed promise for controlling multiple Colletotrichum species. Several
Bio stimulation & Bio remediation Through Rhizosphere TechnologyVIVEK YADAV
This document discusses bio-stimulation and bioremediation through rhizosphere technology. It defines plant growth-promoting rhizobacteria (PGPR) as bacteria that live in soil and around plant roots that promote plant growth. PGPR can increase plant growth directly through nutrient fixation, solubilization, and phytohormone production or indirectly through stimulating the plant's immune system and reducing stress. The document explores the various modes of action PGPR use to promote plant growth including altering plant hormone levels, increasing nutrient availability, and protecting against pathogens. It also examines using PGPR to remediate contaminated soils and provides examples of field studies demonstrating the effects of different PGPR on increasing the yield of crops like fenugreek
Ajayasree T. S. Seminar ppt (Microbiome engineering)Ajayasree TS
Microbiome engineering aims to manipulate plant and soil microbiomes to optimize agricultural functions like stress tolerance, growth promotion, and phytoremediation. Techniques include transferring native or synthetic microbiomes to seeds, seedlings, or soil. This can improve drought tolerance, disease resistance, and heavy metal accumulation. Challenges include understanding complex microbiome dynamics and interactions under field conditions. Microbiome engineering shows potential to develop sustainable agriculture through balanced, beneficial microbiome compositions.
CRISPR Cas technology in Plant Disease Management.pptxARUNAT18
This document discusses the use of CRISPR Cas technology in plant disease management. It begins with an introduction to CRISPR Cas technology, describing how it was originally discovered as a prokaryotic antiviral defense system. It then discusses how CRISPR Cas technology works and its various components. The majority of the document focuses on examples of how CRISPR Cas technology has been used to manage bacterial, fungal, and viral diseases in various crop plants by targeting genes involved in disease susceptibility or pathogenesis. It concludes by discussing some advantages and challenges of CRISPR Cas technology and its future applications in advanced crop breeding and plant pathology.
Fungal biocontrol agents like Trichoderma spp. can induce systemic resistance in plants against pathogens through several mechanisms. They compete with pathogens for space and nutrients, produce antibiotics, or act as mycoparasites against other fungi. Trichoderma elicits plant defenses by inducing the expression of pathogenesis-related proteins and antioxidant enzymes. Case studies showed Trichoderma reduced disease severity in cotton, barley, and maize by triggering the plants' terpenoid defenses or increasing their tolerance to salt and drought stress. Fungal biocontrol is an environmentally sustainable alternative to chemicals for managing plant diseases.
Biological Control of Post-harvest Disease of Blue Mould (Penicillium expansu...AymenIsmaelAhmed
Biological Control of Post-harvest Disease of Blue Mould (Penicillium expansum) of Pear Fruit by using Antagonist Microorganisms under Laboratory and Cold Storage Conditions
This document discusses bacteriophages and their applications in food. It begins with an introduction to bacteriophages, their classification, life cycle and genomic structure. It then discusses how bacteriophages can be used as biocontrol agents for foodborne pathogens in various industries and products. It also reviews some studies on bacteriophages for controlling foodborne pathogens. Large-scale production and purification strategies are described. Some companies involved in bacteriophage products are listed, along with advantages and challenges of using bacteriophages. Applications of bacteriophages in detection of pathogens are also summarized.
Role of abiotic stress and improved varieties on fruit productionSamsonAbiola1
This document discusses abiotic and biotic stresses that impact fruit production. It defines plant stress and describes different types of stresses such as water stress, temperature stress, light stress, and interactions with microbes. The document reviews literature on how these stresses affect various fruits and crops. It also discusses management practices and biotechnological tools that can be used to alleviate abiotic stress and improve traits such as drought tolerance and disease resistance in fruit crops to enhance food security. The conclusion states that plant biotechnology has potential to improve long-term production of fruit crops through genetic manipulation of vegetatively propagated plants.
Evaluate the Efficiency of Gamma Irradiation and Chitosan on Shelf-Life of St...IJEABJ
Chitosan play an important role as an antifungal against Botrytis cinerea and the effect was a concentration dependent. The obtained results of in vitro experiment demonstrated that chitosan (4%) decreased radial growth of B. cinereato 2 %. Invivo the severity of infection reduced from 59.8 and 100.0 to 9.7, 33.8 and 40.1 in first, second and third week’s storage periods at 13C, respectively. Also, chitosan coating (4%) significantly caused an increase in fruit firmness whereas TSS was decreased with an increase by increasing in storage time. However,Vitamin C gave fluctuated results by increasing storage time. Gamma irradiation at 2.5 KGy reduced severity (%) of infected fruits from 55.5, 100 and 100 to 31.7, 45.9 and 49.9 and in healthy fruits severity (%) reduced from 48.9, 100 and 100 to 23.3, 25.1 and 29.1 in different storage periods 1, 2 and 3 weeks, respectively. Similarly, chitosan as well as gamma irradiation combination induced a significant increase of peroxidase enzyme (POD) activity. Induced changes in surface morphology and damage of cell structure caused by using chitosan shown by scanning electron microscopy. Also, gamma irradiation causes changes in hyphea structure and in surface morphology but combination of gamma irradiation with chitosan was more effective in altering fungus morphology and cell structure damage and no spore forming. This providing the efficiency of combination on reducing disease severity (%) of strawberry.
Autophagy and its role in plants - By Tilak I S, Dept. of Biotechnology, UASD.Tilak I S
Autophagy (Macroautophagy) a term from the Greek ‘auto’ (self) and ‘phagein’ (to eat), is a highly regulated cellular degradation and recycling process, conserved from yeast to more complex eukaryotes. The process involves sequestration of the cytoplasm into double-membrane vesicles called autophagosomes, which subsequently fuse with lysosomes or vacuoles. The products of autophagic degradation of intracellular material are exported from lysosomes into the cytoplasm where they are recycled (Tang et al., 2018).
Autophagy is activated during various extracellular or intracellular factors such as nutrients deprivation, drought, stresses, and pathogenic invasion to degrade damaged, denatured, and aggregated proteins (Floyd et al., 2015). The mechanism of autophagy induction and regulation is carried out by TOR (Target of Rapamycin) complex and a number of autophagy related genes (ATGs) and proteins which have been identified in higher eukaryotes including yeasts, mammals, and plants (arabidopsis, rice, wheat, tomato and maize etc.) (Ryabovol and Minibayeva., 2016). In plants autophagy is essential for various physiological processes like growth and development, elimination of toxic compounds from the plants Eg: ROS (reactive oxygen species), involved in programmed cell death, nutrients recycling under detrimental environmental factors. Li et al. (2015) transferred an autophagy-related gene, SiATG8a, from foxtail millet to arabidopsis. Through expression profile analyses demonstrated that SiATG8a expression was induced by both drought and nitrogen starvation and over-expression of SiATG8a improved tolerance to nitrogen starvation and drought stress in transgenic Arabidopsis.
The study of autophagy in crop species has been expanding rapidly. Functions of autophagy in development, abiotic stress responses and plant–microbe interactions have been deciphered in various species (Kabbage et al., 2013). New findings such as the involvement of autophagy in reproductive development are increasing our understanding of autophagy but much work is still needed. One interesting topic that warrants more attention is the role of autophagy in organs or tissues that are specifically present in certain crops, for example fruits and nodules.
Considering its importance in development and stress responses, autophagy is a promising target to manipulate for agricultural benefits like higher yield. Increased expression of ATG genes may be valuable in agricultural applications, as this can confer a number of benefits to plants, including enhanced growth, higher yield and increased stress tolerance.
This document discusses plant biopharming, which involves producing recombinant proteins in transgenic plants. It provides an overview of the concept, strategies used, production systems, downstream processing, applications including monoclonal antibodies and edible vaccines, case studies, and biosafety issues. Specifically, it summarizes that plant biopharming is a promising approach for the large-scale, low-cost production of pharmaceuticals due to plants' high protein yields and stability. However, further work is still needed to maximize protein expression, improve purification techniques, evaluate optimal dosages, and enhance biosafety systems to ensure human and environmental safety.
Efficacy of Microbial Biopesticide Formulations in the control of Xanthomonas...Open Access Research Paper
The cashew tree (Anacardium occidentale L.) occupies an important place in the world because of its cashew nut. However, its cultivation is confronted with bacteriosis, a bacterial disease caused by Xanthomonas citri pv. Mangiferaeindicae. This disease is one of the main causes of the low yield per hectare of cashew nuts, which fluctuates between 350 and 500 kg/ha. In view of this, it is wise to find ways of controlling this disease. It is in this context the objective of this work was to produce bio-formulations based on bacteria isolated from the rhizosphere of cashew trees, in order to evaluate their effectiveness on the growth of the agent responsible for cashew bacteriosis (Xanthomonas citri pv. Mangiferaeindicae). Thus, two liquid formulations were made from Pseudomonas fluorescens and Bacillus subtilis isolated from the rhizosphere of cashew. Stability, in vitro antagonism and biocontrol tests against Xanthomonas citri pv. Mangiferaeindicae were performed. The results obtained showed an inhibition of the Xanthomonas citri pv. Mangiferaeindicae bacterium with inhibition zones of 8.13 ± 2.1 and 25.20 ± 3.9 mm in diameter respectively for the products formulated with Bacillus subtilis and Pseudomonas fluorescens. In biocontrol tests, both formulated products showed their ability to protect cashew plants against bacterial blight with reduction rates of 80.95 ± 2.3 % and 73.80 ± 5.2% for the Pseudomonas fluorescens and Bacillus subtilis formulations, respectively. These two formulations of bacterial, once tested in cashew plantations, could be used in the biological control of cashew bacterial blight in Côte d’Ivoire.
This document summarizes a study that evaluated the antifungal activity of fractions isolated from the hexane extract of Acacia nilotica bark. Crude hexane extract and 11 fractions were tested against three fungal pathogens (Alternaria brassicae, Fusarium oxysporum, Rhizoctonia solani). Fraction 2 showed the highest inhibition of mycelial growth for A. brassicae and R. solani, while Fraction 1 was most effective against F. oxysporum. Fraction 2 demonstrated the strongest overall antifungal activity. The antifungal properties may be due to compounds such as carbohydrates, sterols, triterpenoids and anthraquinone present in the
Pharmaceutical Biotechnology on Modern Technological PlatformAshikur Rahman
This document discusses how modern biotechnology influences technological platforms. It explains that biotechnology uses living organisms to develop useful products through techniques like recombinant DNA and genetic engineering. These allow genes to be moved between organisms, influencing their traits. The document provides examples of applications in healthcare, agriculture, industry, and the environment. It also describes different branches of biotechnology like bioinformatics, green biotechnology, red biotechnology, and white biotechnology. Finally, it discusses how genetic engineering can help crops gain resistance to diseases and insects, reducing the need for pesticides and helping crops withstand harsh conditions.
This document discusses plant biopharming, which involves producing recombinant proteins in transgenic plants. It provides an overview of the concept, strategies, production systems, applications and case studies of plant biopharming. Specifically:
1. Plant biopharming is more cost effective than traditional systems, with transgenic plants able to produce proteins on a large scale. Common plants used include tobacco, cereals and potatoes.
2. Stable nuclear transformation is the most common method to generate transgenic plants. Applications include producing monoclonal antibodies, industrial enzymes, and edible vaccines in plants.
3. A case study demonstrates the production of highly concentrated and heat-stable hepatitis B surface antigen in transgenic maize, with the
This document outlines a presentation on plant biopharming. It discusses the use of transgenic plants to produce therapeutic proteins and some key points:
- Biopharming involves using transgenic plants to produce proteins of therapeutic value. It started about 20 years ago with the promise to produce expensive molecules cheaper.
- Different production systems are discussed, including stable nuclear transformation, plastid transformation, transient transformation, and stable hydroponic transformation. Tobacco, lettuce, alfalfa, rice and maize are common plant species used.
- Applications include pharmaceuticals, industrial enzymes, monoclonal antibodies, edible vaccines. Successful reports demonstrate production of measles virus protein in transgenic carrot and human papillomavirus protein
Similar to Role of chitosan in Post Harvest Disease Management (20)
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...AbdullaAlAsif1
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
BREEDING METHODS FOR DISEASE RESISTANCE.pptxRASHMI M G
Plant breeding for disease resistance is a strategy to reduce crop losses caused by disease. Plants have an innate immune system that allows them to recognize pathogens and provide resistance. However, breeding for long-lasting resistance often involves combining multiple resistance genes
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
ANAMOLOUS SECONDARY GROWTH IN DICOT ROOTS.pptxRASHMI M G
Abnormal or anomalous secondary growth in plants. It defines secondary growth as an increase in plant girth due to vascular cambium or cork cambium. Anomalous secondary growth does not follow the normal pattern of a single vascular cambium producing xylem internally and phloem externally.
The debris of the ‘last major merger’ is dynamically youngSérgio Sacani
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
2. Dept. of Plant Pathology 2
Importance of fruits and vegetables
(Jideani et al., 2021)
Antioxidants are important ingredients present in fruits
and vegetables
3. Dept. of Plant Pathology 3
What is post harvest diseases
The diseases which develop on harvested parts of the plants
like seeds, fruits and also on vegetables are known as post-
harvested diseases
(Wan et al., 2021)
14. 14
Dept. of Plant Pathology
Role of Chitosan in Post harvest Disease
Management
Arun A.T.
2020-21-011
Dept. of Plant Pathology
15. Dept. of Plant Pathology
1
5
1. Introduction
2. What is chitosan
3. Importance of chitosan in post harvest disease management
4. Management of bacterial diseases by chitosan
5. Mechanism of action of bacterial disease management
6. Management of fungal diseases by using chitosan
7. Mechanism of action of fungal disease management
8. Effect of chitosan coating on physiological quality parameters
9. Problems associated with the usage of chitosan
10. Conclusion
11. Future perspective
Contents
16. Dept. of Plant Pathology 16
What is chitosan
Chitosan is a high molecular weight cationic
polysaccharide consisting of β-(1-4)-linked D-glucosamine
(deacetylated unit) and N-acetyl-D-glucosamine and usually
refers to a family of chitin derivatives obtained after partial
deacetylation
(Haghighi et al., 2020)
17. Dept. of Plant Pathology 17
NHCOCH3
NH2
Difference between chitin and chitosan
Chitin Chitosan
(Bibi et al., 2021)
18. Dept. of Plant Pathology 18
Prof. C. Rouget
(1859)
Discovery of chitosan
(Torres-Rodriguez et al., 2021)
19. 19
Dept. of Plant Pathology
Sources of chitosan
Fungal cell walls
Insect exoskeletons
Crustacean shells
Crab
shell
Shrimp
shell
(Yang et al., 2021)
20. Dept. of Plant Pathology 20
Extraction of chitosan
1.Biological method
2.Chemical method
Different Methods
(Varun et al., 2017)
21. Dept. of Plant Pathology 21
Major steps
1.Demineralization
2.Deproteination
3.Deacetylation
Extraction of chitosan
(Varun et al., 2017)
22. Dept. of Plant Pathology (Schmitz et al., 2019)
Biological method
Shrimp
shell waste
Crude shell
material
Demineralized
shell material
Chitin
Deproteinated
shell waste
Chitosan
Washing and drying
Lactic acid bacteria
Deacetylases
Proteolytic bacteria
Deminaralization
Deproteination
Deacetylation
22
23. 23
Dept. of Plant Pathology
Chemical method
Shrimp
shell waste
Deminaralized
powder
Chitin
Chitosan
Washing and drying
2N HCl (1:15), 2h,
150 rpm
2N NaoH (1:20), 2h, 150
rpm at 50°C
50%
NaOH, 1h
at 121°C,
15 psi
Demineralization
Deproteination
Deacetylation
Shrimp shell
powder
(Varun et al., 2017)
24. Dept. of Plant Pathology 24
Various applications of chitosan
Agriculture
Food industry
Pharmaceutical industry
Cosmetic industry
Water treatment
Paper industry
(Sigroha and Khatkar, 2017)
25. Dept. of Plant Pathology 25
Application of chitosan in agriculture
Biopesticide
Growth promoter
Post harvest disease management
(Bandara et al., 2020)
26. 26
Dept. of Plant Pathology
Importanant charecteristics of chitosan
Antioxidant
Biodegradability
Chemical stability
Antimicrobial
Non-toxicity
Film-forming
(Haghighi et al., 2020)
27. Dept. of Plant Pathology 27
Chitosan treatment -fresh products - safe -consumer &
environment
Chitosan - approved
United State Food and Drug Administration (USFDA)
Generally Reconized as Safe (GRAS) food additives
(Bibi et al., 2021)
28. Dept. of Plant Pathology 28
Preparation of chitosan solution
1. Chitosan solutions were prepared by dissolving Chitosan (1%
(w/v)) in 0.25N HCl.
2. The solution was centrifuged to remove undissolved particles
and the pH was adjusted to 5.6 with 1N NaOH.
At this pH, Chitosan is positively charged and exhibits
maximal biological activity.
(Prabha and Sivakumar, 2017)
29. Dept. of Plant Pathology 29
Dipping method of chitosan application
(Romanazzi, 2010)
30. Dept. of Plant Pathology 30
Product trade name Company (Country) Formulation Active
ingredient (%)
Chito plant ChiPro GmbH (Bremen, Germany Powder 99.9
Chito plant ChiPro GmbH (Bremen,
Germany)
Liquid 2.5
OII-YS Venture Innovations (Lafayette,
LA, United States)
Liquid 5.8
KaitoSo Advanced Green
Nanotechnologies Sdn Bhd
(Cambridge, United Kingdom)
Liquid 12.5
Armour-Zen Botry-Zen Limited (Dunedin,
New Zealand)
Liquid 14.4
Biorend Bioagro S.A. (Chile) Liquid
Kiforce Alba Milagro (Milan, Italy) Liquid 6
(Romanazzi et al., 2018)
Commercial products of chitosan
1.25
31. Dept. of Plant Pathology 31
Product trade name Company (Country) Formulation Active
ingredient (%)
FreshSeal BASF Corporation (Mount Olive,
NJ, United States)
Liquid 2.5
ChitoClear Primex ehf (Siglufjordur,
Iceland)
Powder
Bioshield Seafresh (Bangkok, Thailand) Powder
Biochikol 020 PC Gumitex (Lowics, Poland) Liquid 2
Kadozan Lytone Enterprise, Inc. (Shanghai
Branch, China)
Liquid 2
Kendal cops Valagro (Atessa, Italy) Liquid 4
Chitosan 87% Korea Chengcheng Chemical
Company (China)
TC (Technical
material)
87
Commercial products of chitosan
(Romanazzi et al., 2018)
100
100
32. 32
Dept. of Plant Pathology
pH
pH
Concentration
Molecular weight
Degree of deacetylation
Derivatives of chitosan
Type of organisms
Source of chitosan
Chitosan complexes
Factors affecting microbial activity of chitosan
(Ke et al., 2021)
33. Dept. of Plant Pathology 33
pH
Higher antimicrobial activity at low pH; Ideal pH ≤ 6
Anti
microbial
activity
(Kravanja et al., 2019)
pH
34. Dept. of Plant Pathology 34
Concentration
Effects of chitosan concentration on spore germination (A) and germ tube
elongation (B) of Botrytis cinerea and Penicillium expansum 12 h after
incubation at 25 °C.
(Liu et al., 2007)
35. Dept. of Plant Pathology 35
Molecular weight
Effect of low molecular weight chitosan (LMWC) and high molecular weight
chitosan (HMWC) on decay of citrus fruits caused by Penicillium digitatum,
Penicillium italicum, Botrydiplodia lecanidion and Botrytis cinerea
(Zhang et al., 2011)
HMWC
LMWC
36. Dept. of Plant Pathology 36
Degree of deacetylation (DD)
Degree of deacetylation Class Property
70–85% Middle Partly dissolved in water
85–95% High Good solubility in water
95–100% Ultrahigh Excellent solubility in
water
(Zhuang et al., 2019)
37. Dept. of Plant Pathology 37
Derivatives of chitosan
Carboxymethyl chitosan
Quaternized carboxymethyl chitosan
(Sun et al., 2006)
38. Dept. of Plant Pathology 38
Effect of chitosan and oligochitosan with different concentrations on brown rot
diseases of peach fruit stored at 25 0C after 4 days
(Ma et al., 2013)
Role of chitosan and oligochitosan on peach fruit decay
39. Dept. of Plant Pathology 39
Type of organisms
Bacteria generally less sensitive to the antimicrobial action of
chitosan than fungi
(Kong et al., 2010)
40. Dept. of Plant Pathology 40
Source of chitosan
Fungal chitosan exhibited low antimicrobial activity as
compared to what crustacean shell chitosan
(Jeihanipour et al., 2007)
42. Dept. of Plant Pathology 42
Reduction in the linear growth and spore germination (%) of Penicillium
digitatum and P.italicum in citrus fruits as affected by different concentrations
of chitosan, lemongrass and citral essential oils on PDA medium
(El-Mohamedy et al., 2015)
43. Dept. of Plant Pathology 43
Chitosan
+Ve
-Ve
+Ve
-Ve
Electrostatic interaction
General antimicrobial action of chitosan
Pathogen
(Xing et al., 2015)
44. Chitosan-DNA/RNA interactions
• Chitosan able to pass through the microbial cell membrane
44
Dept. of Plant Pathology
mRNA
Proteins
Chitosan
(Xing et al., 2015)
45. Dept. of Plant Pathology 45
Management of bacterial diseases by using chitosan
The in vitro antibacterial activity of different molecular weights of chitosan products
against A. tumefaciens, C. fascians, E. carotovora, and P. solanacearum and in
combination with different concentrations of geraniol and thymol by nutrient agar (NA)
dilution technique.
47. Dept. of Plant Pathology
47
Photograph of the in vitro growth of A. tumefaciens, C. fascians, E.
carotovora, and P. solanacearum in NA plates incorporated with
chitosan film enriched with thymol (0.5%)
(Badawy et al., 2016)
48. Dept. of Plant Pathology 48
Mechanism of action of bacterial disease management
Differences in the cell surface structure G +ve and G -ve bacteria
-distinct susceptibilities to chitosan.
(Pasquina-Lemonche et al., 2020)
49. 49
Dept. of Plant Pathology
Antimicrobial activity against G +ve bacteria
(Ke et al., 2021)
50. 50
Dept. of Plant Pathology
Antimicrobial activity against G -ve bacteria
(Ke et al., 2021)
51. Dept. of Plant Pathology 51
Management of fungal diseases by using chitosan
52. Dept. of Plant Pathology 52
Effect of chitosan on mycelial growth of Colletotrichum capsici
(Akter et al., 2018)
Treatments Average mycelial growth
after 10 days (mm)
% of mycelial growth
inhibition over control
Control 90.00 a -
0.4% chitosan 64.30 b 28.56
0.6% chitosan 36.70 c 59.22
0.8% chitosan 11.00 d 87.78
1% chitosan 0.00 e 100.00
1.2% chitosan 0.00 d 100.00
53. Dept. of Plant Pathology 53
Mycelial growth inhibition of C. capsici by chitosan on PDA
Control 0.6% chitosan 0.8% chitosan 1% chitosan
(Akter et al., 2018)
54. Dept. of Plant Pathology 54
In vitro development of three chitosan-treated isolates of
Colletotrichum obtained from soursop, mango and banana held
at different concentrations and incubated at 20 ± 2ºC
(Gutierrez-Martinez et al., 2017)
55. Dept. of Plant Pathology 55
Mechanism of action of fungal disease management
56. 56
Dept. of Plant Pathology
Antimicrobial activity against fungi
Fungi
(Ke et al., 2021)
57. Dept. of Plant Pathology 57
Effect of chitosan on spore germination
Penicillium expansum
(Li et al., 2019)
58. Dept. of Plant Pathology 58
Effect of chitosan on fungal growth
Penicillium expansum
(Li et al., 2019)
59. Dept. of Plant Pathology 59
Morphological changes in response to chitosan
Light micrographs of P. expansum mycelia after 7 days of
cultivation with or without 0.05% chitosan treatment
(Li et al., 2019)
60. Dept. of Plant Pathology 60
Transmission electron micrographs of P. expansum conidia after
6 h of cultivation with or without 0.05% chitosan treatment
61. Dept. of Plant Pathology 61
Effect of chitosan coating on physiological quality
parameters
Total soluble solid
Fungal decay
Weight loss
Firmness
Respiration
(Aziz et al., 2021)
62. Dept. of Plant Pathology 62
Respiration
Changes in respiration rate of plum fruits coated with Chitosan during
cold storage.
(Bal, 2013)
63. Dept. of Plant Pathology 63
Weight loss
Change in weight loss of fresh-cut mangoes stored at 6°C
(Nongtaodum and Jangchud, 2009)
64. Dept. of Plant Pathology 64
Firmness
Effects of chitosan treatments on firmness of banana (cv. Sabri)
during 8 days after storage
(Aziz et al., 2021)
A Firmness scores:
1 = hard green
2 = sprung
3 = between sprung and
eating ripe
4 = eating ripe
5 = over ripe
6 = Blackened / rotten.
T0: Control
T1: 0.50% Chitosan
T2: 0.75% Chitosan
T3: 1.0% Chitosan
T4: 1.5% Chitosan
T5: 2.0% Chitosan
65. Dept. of Plant Pathology 65
Fungal decay
(Lin et al., 2011)
66. Dept. of Plant Pathology 66
Total soluble solid(TSS)
Effect of chitosan coating on TSS of banana fruit
(Hossain and Iqbal, 2016)
67. Dept. of Plant Pathology
67
(Prabha and Sivakumar, 2017)
Effect of chitosan treatments on shelf life of capsicum at
different concentration
4 Fourth
Day
Wrinkle were formed and size
began to shrink
No Change No Change
68. Dept. of Plant Pathology 68
(Prabha and Sivakumar, 2017)
69. Dept. of Plant Pathology 69
Day 1
Day 3
Day 2
Day 4
(Prabha and Sivakumar, 2017)
Effect of chitosan treatments on shelf life of capsicum at
different concentration
Control; 1% Chitosan; 3% Chitosan
70. Dept. of Plant Pathology 70
Day 5 Day 6
Day 7 Day 8
(Prabha and Sivakumar, 2017)
Control; 1% Chitosan; 3% Chitosan
Effect of chitosan treatments on shelf life of capsicum at
different concentration
71. Dept. of Plant Pathology 71
Effect of chitosan on tomato
(Prabha and Sivakumar, 2017)
72. Dept. of Plant Pathology 72
Day 1 Day 2
Day 3 Day 4
(Prabha and Sivakumar, 2017)
Control; 1% Chitosan; 3% Chitosan
Effect of chitosan treatments on shelf life of tomato at
different concentration
73. Dept. of Plant Pathology 73
(Prabha and Sivakumar, 2017)
Day 5 Day 6
Day 7 Day 8
Control; 1% Chitosan; 3% Chitosan
Effect of chitosan treatments on shelf life of capsicum at
different concentration
74. Dept. of Plant Pathology 74
Problems associated with the usage of chitosan
•Molecular weight
•Purity
•Solubility
•Its characteristics and biodiversity
• Not sufficient data on different effects on the fungi
affecting the fruits or vegetables
(Verlee et al., 2017)
75. Dept. of Plant Pathology 75
Conclusion
•Chitosan, naturally occurring compound, possessing broad-spectrum
antimicrobial effects potential in agriculture with regard to controlling
post harvest diseases
•Its application may counteract the wide use of chemical pesticides, in
part at least
•The polysaccharide chitosans represent a renewable source of natural
biodegradable polymers and meet with the emergence of more and
more food safe problem
76. Dept. of Plant Pathology 76
Future perspective
•The mechanisms of growth inhibition of pathogens and induced plant
immunity is unclear
• Chemical modification - enhance its antimicrobial activities, improve
the physical and chemical properties, and make it more suitable for
field applications
•In the case of antimicrobial mode of action, future work should aim at
clarifying the actual target molecule on the cell surface or other
intracellular targets