Rice research intitiative
perspectives
GCP General Research Meeting
September 27 – 30, 2013
Lisbon, Portugal
Rice group parallel discussions
• Session 1 (September 27)
– Review of product catalogue
– Identification of output products to come (before
and after the end of 2014)
• Session 2 (September 28)
– Data Management strategy and crop DB
– Classification of projects into finished (currently
and by end of 2014) and unfinished activities
Rice product catalogue
Genomic Resources (13)
- Large marker (SNP, SSR) dataset for diversity analysis 10
- Candidate genes 3
Germplasm (27)
- Improved variety 5
- Improved donor 9
- Genetic stock with high resolution power for genetic analysis 13
Marker for MAS (13)
- Salinity tolerance QTL 4
- Aluminum tolerance QTL 1
- Phosphorus uptake QTL 1
- Drought tolerance QTL 3
- Blast resistance gene 2
- Bacterial Blight resistance gene 2
Protocol (detection of deleted regions in rice mutants) 1
Training material (Marker Assisted Selection) 1
Informatics tool (APSIM module, OryGenesDB/reverse genetics, …) 4
Total 59
Genomic resources
• Marker data set/data base
– Data base of datasets for 420,000 SNPs
– Data set SNP for rice
– SNPs for identifying haplotype structure
– Arrays for SNP discovery in rice
– SSR marker kit for diversity analysis
– Anchor markers for inter-specific crosses
• Candidate genes
– Dataset of non-host genes for IR64 & Nipponbare varieties
– Transcriptome dataset for drought stress
– Drought responsive genes.
Germplasm
• Improved varieties
– Liaoyou 5224, new Chinese hybrid rice with drought
tolerance
– Manawthukha, improved cooking quality, Myanmar, released
2011
– Sinthu Latt, salt tolerance for Myanmar
– BRRI dhan28-Saltol and BR11-Saltol for India
– Vandana_DT and IR64_DT for India
• Genetic stock with high resolution power
– Chromosome segment substitution lines (CSSL) O. sativa x O.
glaberrima
– CSSL populations of O. sativa x Oryza species with A genome
– Nested Association Mapping (NAM) population
– Multiparent advanced generation intercrosses (MAGIC)
populations, indica, japonica, …
Additional work on product catalogue
• Completeness /missing products
– Achieved projects:
• I bridge lines developed from the crosses of 30 O. representative accessions of O.
glaberrima and 3 recipient O. sativa varieties, Curinga, Wab165 and IR64.
• ………….
– On-going projects
• Rice challenge initiative
– Map of drought prevalence and severity in the rain fed lowland ecosystem of, Burkina, Mali , an
Nigeria
– Germplasm
• Breeding DT / lowland rice/ Mekong region
– Map of drought prevalence and severity in the rain fed lowland ecosystem of, Cambodia, Laos,
Myanmar
– Germplasm
• ……………
• Products documentation
– More detailed description : 200-250 words (format of summary of scientific
publication), including IP issues
– More sustainable access pathway
• Germplasm : gene-bank accession number (5-10 years availability)
• Dataset : actually accessible database
• All product : double entry points, individual scientist + permanent institutional body
Prospects for on-going projects
a: need for financial support for the final step of product delivery ; b: potential home/support after 2014
Projects
2007
2008
2009
2010
2011
2012
2013
2014
2015
2016
a b
G4008.45 NAM population GRiSP
G7009.07 Cloning AltSB/Al tolerance ?
G3008.06 Root traits / productivity /water-limited GRiSP
GRiSP
GRiSP
GRiSP
GRiSP
GRiSP,
M&BG
, …,
?
Mapping DT_QTL major effectG3007.05
Dissemination DT_QTLs pyramided linesG4011.04
Breeding DT/GIS_lowland/Mekong regionG3008.09
MARS / DT & yield potential / West AfricaG7010.04
MAGIC populations
G4010.04
G4010.01
G4011.07
Community/Pyramiding QTL/Mekong regionG4009.09
Novel QTLs / QTL Pyramiding / salinity & sub.
Data management
Projects
Data
Pedigree Genotype Phenotype Other
G3007.05 Mapping DT_QTL major effect Puplication
G4011.04 Dissemination DT_QTLs pyramided lines IRIS IRIS IRIS
G3008.09 Breeding DT / lowland rice/ Mekong region GCP GCP GCP
G7010.04 MARS / DT & yield potential / West Africa IBP IBP IBP AgTria ?
G4009.09 Community /QTL pyrmiding/ Mekong region GCP GCP GCP AgTria ?
G4011.07 MAGIC populations (~ 6 000 lines) IRIS IRIS IRIS
G4008.45 NAM population (~ 4 000 lines). IRIS IRIS IRIS
G3008.06 Root traits / productivity /water-limited IRIS
G7009.07 Cloning AltSB/Al tolerance
G4010.04
Novel QTLs / QTL pyrmiding / salinity & sub IRIS IRIS IRIS
Homework
• Product catalogue
– Completing the list with missing products
• From achieved projects
• From on-going projects
– 250 words description of major products
– Sustainable access pathway to products
• Data management
– Description of the forthcoming data sets
– Time table for delivery
• Prospects for on-going projects
– Short request-note for additional financial support from
GCP to ensure delivery of forthcoming product (if needed)
– Short concept-note for GCP’s support / recommendation
to potential home institution / donor for new phase of the
project (if needed)

GRM 2013: Rice product catalogue and project status -- Projects ongoing, completed, and to be continued post-2014 -- N Ahmadi

  • 1.
    Rice research intitiative perspectives GCPGeneral Research Meeting September 27 – 30, 2013 Lisbon, Portugal
  • 2.
    Rice group paralleldiscussions • Session 1 (September 27) – Review of product catalogue – Identification of output products to come (before and after the end of 2014) • Session 2 (September 28) – Data Management strategy and crop DB – Classification of projects into finished (currently and by end of 2014) and unfinished activities
  • 3.
    Rice product catalogue GenomicResources (13) - Large marker (SNP, SSR) dataset for diversity analysis 10 - Candidate genes 3 Germplasm (27) - Improved variety 5 - Improved donor 9 - Genetic stock with high resolution power for genetic analysis 13 Marker for MAS (13) - Salinity tolerance QTL 4 - Aluminum tolerance QTL 1 - Phosphorus uptake QTL 1 - Drought tolerance QTL 3 - Blast resistance gene 2 - Bacterial Blight resistance gene 2 Protocol (detection of deleted regions in rice mutants) 1 Training material (Marker Assisted Selection) 1 Informatics tool (APSIM module, OryGenesDB/reverse genetics, …) 4 Total 59
  • 4.
    Genomic resources • Markerdata set/data base – Data base of datasets for 420,000 SNPs – Data set SNP for rice – SNPs for identifying haplotype structure – Arrays for SNP discovery in rice – SSR marker kit for diversity analysis – Anchor markers for inter-specific crosses • Candidate genes – Dataset of non-host genes for IR64 & Nipponbare varieties – Transcriptome dataset for drought stress – Drought responsive genes.
  • 5.
    Germplasm • Improved varieties –Liaoyou 5224, new Chinese hybrid rice with drought tolerance – Manawthukha, improved cooking quality, Myanmar, released 2011 – Sinthu Latt, salt tolerance for Myanmar – BRRI dhan28-Saltol and BR11-Saltol for India – Vandana_DT and IR64_DT for India • Genetic stock with high resolution power – Chromosome segment substitution lines (CSSL) O. sativa x O. glaberrima – CSSL populations of O. sativa x Oryza species with A genome – Nested Association Mapping (NAM) population – Multiparent advanced generation intercrosses (MAGIC) populations, indica, japonica, …
  • 6.
    Additional work onproduct catalogue • Completeness /missing products – Achieved projects: • I bridge lines developed from the crosses of 30 O. representative accessions of O. glaberrima and 3 recipient O. sativa varieties, Curinga, Wab165 and IR64. • …………. – On-going projects • Rice challenge initiative – Map of drought prevalence and severity in the rain fed lowland ecosystem of, Burkina, Mali , an Nigeria – Germplasm • Breeding DT / lowland rice/ Mekong region – Map of drought prevalence and severity in the rain fed lowland ecosystem of, Cambodia, Laos, Myanmar – Germplasm • …………… • Products documentation – More detailed description : 200-250 words (format of summary of scientific publication), including IP issues – More sustainable access pathway • Germplasm : gene-bank accession number (5-10 years availability) • Dataset : actually accessible database • All product : double entry points, individual scientist + permanent institutional body
  • 7.
    Prospects for on-goingprojects a: need for financial support for the final step of product delivery ; b: potential home/support after 2014 Projects 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 a b G4008.45 NAM population GRiSP G7009.07 Cloning AltSB/Al tolerance ? G3008.06 Root traits / productivity /water-limited GRiSP GRiSP GRiSP GRiSP GRiSP GRiSP, M&BG , …, ? Mapping DT_QTL major effectG3007.05 Dissemination DT_QTLs pyramided linesG4011.04 Breeding DT/GIS_lowland/Mekong regionG3008.09 MARS / DT & yield potential / West AfricaG7010.04 MAGIC populations G4010.04 G4010.01 G4011.07 Community/Pyramiding QTL/Mekong regionG4009.09 Novel QTLs / QTL Pyramiding / salinity & sub.
  • 8.
    Data management Projects Data Pedigree GenotypePhenotype Other G3007.05 Mapping DT_QTL major effect Puplication G4011.04 Dissemination DT_QTLs pyramided lines IRIS IRIS IRIS G3008.09 Breeding DT / lowland rice/ Mekong region GCP GCP GCP G7010.04 MARS / DT & yield potential / West Africa IBP IBP IBP AgTria ? G4009.09 Community /QTL pyrmiding/ Mekong region GCP GCP GCP AgTria ? G4011.07 MAGIC populations (~ 6 000 lines) IRIS IRIS IRIS G4008.45 NAM population (~ 4 000 lines). IRIS IRIS IRIS G3008.06 Root traits / productivity /water-limited IRIS G7009.07 Cloning AltSB/Al tolerance G4010.04 Novel QTLs / QTL pyrmiding / salinity & sub IRIS IRIS IRIS
  • 9.
    Homework • Product catalogue –Completing the list with missing products • From achieved projects • From on-going projects – 250 words description of major products – Sustainable access pathway to products • Data management – Description of the forthcoming data sets – Time table for delivery • Prospects for on-going projects – Short request-note for additional financial support from GCP to ensure delivery of forthcoming product (if needed) – Short concept-note for GCP’s support / recommendation to potential home institution / donor for new phase of the project (if needed)