This document summarizes work done by CIAT to improve common bean productivity in marginal environments in sub-Saharan Africa. It describes the use of markers for biotic stress resistance, including for diseases like BCMV, bruchid resistance, and common bacterial blight. It also discusses ongoing work on drought tolerance using MARS and MAGIC populations. Data management practices are being improved and capacity building activities with African partners are highlighted. The overall aim is to develop improved germplasm with biotic and abiotic stress resistance to increase yields for smallholder farmers in Africa.
CRISPR Is On The Move: Genome Editing From Rice To WheatFabio Caligaris
Presented at Plant Genomics and Gene Editing Congress: Europe. For more information visit: www.global-engage.com
Success in rice is not equalled in wheat: CRISPR/Cas9 efficiency is around 5% and requires a time-consuming downstream identification of the genome edited plants.
Targeted Breeding Applications of CRISPR-CasKate Barlow
Doane Chilcoat, Director, Applied Technology Systems, DuPont Pioneer
CRISPR-Cas as an advanced plant breeding tool is a more efficient way to improve plants and help farmers produce more and better food, with fewer resources. The superior properties of CRISPR-Cas allows DuPont Pioneer scientists to develop innovative and sustainable seed products for growers similar to those realized through conventional plant breeding, but with even greater efficiency, accuracy and quality. Pioneer is leading the application of this tool to develop customized agriculture solutions. In this talk, potential product targets of this promising technology will be discussed. Approaches to fostering social license and developing an open innovation model for CRISPR-Cas will also be reviewed.
Editing rice-genome with CRISPR/Cas9: To improve agronomic traits for increa...apaari
Editing rice-genome with CRISPR/Cas9: To improve agronomic traits for increased crop productivity by MK Reddy during the Regional Expert Consultation on Gene Editing in Agriculture and its Regulations Technical Session III
CRISPR Is On The Move: Genome Editing From Rice To WheatFabio Caligaris
Presented at Plant Genomics and Gene Editing Congress: Europe. For more information visit: www.global-engage.com
Success in rice is not equalled in wheat: CRISPR/Cas9 efficiency is around 5% and requires a time-consuming downstream identification of the genome edited plants.
Targeted Breeding Applications of CRISPR-CasKate Barlow
Doane Chilcoat, Director, Applied Technology Systems, DuPont Pioneer
CRISPR-Cas as an advanced plant breeding tool is a more efficient way to improve plants and help farmers produce more and better food, with fewer resources. The superior properties of CRISPR-Cas allows DuPont Pioneer scientists to develop innovative and sustainable seed products for growers similar to those realized through conventional plant breeding, but with even greater efficiency, accuracy and quality. Pioneer is leading the application of this tool to develop customized agriculture solutions. In this talk, potential product targets of this promising technology will be discussed. Approaches to fostering social license and developing an open innovation model for CRISPR-Cas will also be reviewed.
Editing rice-genome with CRISPR/Cas9: To improve agronomic traits for increa...apaari
Editing rice-genome with CRISPR/Cas9: To improve agronomic traits for increased crop productivity by MK Reddy during the Regional Expert Consultation on Gene Editing in Agriculture and its Regulations Technical Session III
Gene editing with CRISPR/Cas9: sorghum as a case studyapaari
Gene editing with CRISPR/Cas9: sorghum as a case study by Ian Godwin during the Regional Expert Consultation on Gene Editing in Agriculture and its Regulations Technical Session I
Research Program Genetic Gains (RPGG) Review Meeting 2021: From Discovery to ...ICRISAT
Chickpea (Cicer arietinum) is the second most widely grown legume crop after soybean, accounting for a substantial proportion of human dietary nitrogen intake and playing a crucial role in food security in developing countries. We report the∼ 738-Mb draft whole genome shotgun sequence of CDC Frontier, a kabuli chickpea variety, which contains an estimated 28,269 genes. Resequencing and analysis of 90 cultivated and wild genotypes from ten countries identifies targets of both breeding-associated genetic sweeps and breeding-associated balancing selection. Candidate genes for disease resistance and agronomic traits are highlighted, including traits that distinguish the two main market classes of cultivated chickpea—desi and kabuli.
CRISPR Agbio San Diego April 2017 AgendaDiane McKenna
CRISPR AgBio Congress is the first and only end-to-end meeting dedicated to helping agricultural biotech ad agrochemical companies leverage the power of CRISPR/Cas9 advanced trait breeding technology and precision genome editing, to overcome productivity challenges, increase yield and pioneer sustainable agriculture in plants breeding, crop protection and livestock. Commercialize the next generation of sustainable and superior agricultural products and help meet the world’s growing food demands.
Research Program Genetic Gains (RPGG) Review Meeting 2021: Groundnut genomic ...ICRISAT
These high quality genomes are global resource and are being used by all the genomics and breeding researchers across the world including ICRISAT. High density genotyping assays developed and currently been deployed for generating high throughput and high density genotyping data on germplasm and breeding lines.
GENESIS™: Comprehensive genome editing - Translating genetic information into personalised medicines.
Horizon is the only source of rAAV expertise and is uniquely capable of exploiting multiple platforms: CRISPR, ZFNs and rAAV singularly or combined. Horizon’s scientists are experts at all forms of gene editing and so have the experience to help guide customers towards the approach that best suits their project
Genome Editing Comes of Age; CRISPR, rAAV and the new landscape of molecular ...Candy Smellie
Information is no longer a bottleneck, emphasis is shifting to the ‘what does it all mean’
In a translational context we hope that by answering that question we will be able to is to characterise the genetics that drive disease, and indeed develop drugs and diagnostics that are personalised to patients.
Genome editing provides the link between the information here, and this outcome here, by allowing scientists to recapitulate specific genetic alterations in any gene in any living tissue to probe function, develop disease models and identify therapeutic strategies. So, not only do we now have unparalleled access to genetic information, but we now have the tools to most accuartely understand what this genetic information – with genome editing allowing us to explore the genetic drivers of disease in physiological models.
AAV is a single-stranded, linear DNA virus with a a 4.7 kb genome which for the purpose of genome editing is replaced almost in entirety with the targeting vector sequence (except for the iTRs)
It is in effect a highly effective DNA delivery mechanism
After entry of the vector into the cell, target-specific homologous DNA is believed to activate and recruit HR-dependent repair factors can induce HR at rates approximately 1,000 times greater than plasmid based double stranded DNA vectors, but the mechanism by which it achieves this is still largely unknown
By including a selection cassette can select for cells that have integrated the targeting vector, and then screen for clones which have undergone targeted insetion rather than random integration, which will generally be around 1%.
Research Program Genetic Gains (RPGG) Review Meeting 2021: From Discovery to ...ICRISAT
A number of advances in genetics and genomics research of pigeonpea. These advances have enhanced our understanding of structural and functional aspects of genome and also provided us opportunities to deal with constraints impeding production of pigeonpea in precise and faster manner. Availability of the draft genome sequence and large-scale molecular markers has made it possible to map traits of interest in speedy manner. Although germplasm re-sequencing has already been started in pigeonpea, large-scale germplasm including elite breeding line, landraces and wild species is expected to be fully sequenced very soon.
Next generation genomics for chickpea (Cicer arietinum L.) improvementICRISAT
Large scale genomic resources including draft genome sequence, re-sequencing of 90 lines, comprehensive transcriptome assembly and high density genetic maps have been developed for chickpea. Linkage mapping and genome wide association studies (GWAS) are being used for trait
mapping.
26 Feb 2014
Gene editing with CRISPR/Cas9: sorghum as a case studyapaari
Gene editing with CRISPR/Cas9: sorghum as a case study by Ian Godwin during the Regional Expert Consultation on Gene Editing in Agriculture and its Regulations Technical Session I
Research Program Genetic Gains (RPGG) Review Meeting 2021: From Discovery to ...ICRISAT
Chickpea (Cicer arietinum) is the second most widely grown legume crop after soybean, accounting for a substantial proportion of human dietary nitrogen intake and playing a crucial role in food security in developing countries. We report the∼ 738-Mb draft whole genome shotgun sequence of CDC Frontier, a kabuli chickpea variety, which contains an estimated 28,269 genes. Resequencing and analysis of 90 cultivated and wild genotypes from ten countries identifies targets of both breeding-associated genetic sweeps and breeding-associated balancing selection. Candidate genes for disease resistance and agronomic traits are highlighted, including traits that distinguish the two main market classes of cultivated chickpea—desi and kabuli.
CRISPR Agbio San Diego April 2017 AgendaDiane McKenna
CRISPR AgBio Congress is the first and only end-to-end meeting dedicated to helping agricultural biotech ad agrochemical companies leverage the power of CRISPR/Cas9 advanced trait breeding technology and precision genome editing, to overcome productivity challenges, increase yield and pioneer sustainable agriculture in plants breeding, crop protection and livestock. Commercialize the next generation of sustainable and superior agricultural products and help meet the world’s growing food demands.
Research Program Genetic Gains (RPGG) Review Meeting 2021: Groundnut genomic ...ICRISAT
These high quality genomes are global resource and are being used by all the genomics and breeding researchers across the world including ICRISAT. High density genotyping assays developed and currently been deployed for generating high throughput and high density genotyping data on germplasm and breeding lines.
GENESIS™: Comprehensive genome editing - Translating genetic information into personalised medicines.
Horizon is the only source of rAAV expertise and is uniquely capable of exploiting multiple platforms: CRISPR, ZFNs and rAAV singularly or combined. Horizon’s scientists are experts at all forms of gene editing and so have the experience to help guide customers towards the approach that best suits their project
Genome Editing Comes of Age; CRISPR, rAAV and the new landscape of molecular ...Candy Smellie
Information is no longer a bottleneck, emphasis is shifting to the ‘what does it all mean’
In a translational context we hope that by answering that question we will be able to is to characterise the genetics that drive disease, and indeed develop drugs and diagnostics that are personalised to patients.
Genome editing provides the link between the information here, and this outcome here, by allowing scientists to recapitulate specific genetic alterations in any gene in any living tissue to probe function, develop disease models and identify therapeutic strategies. So, not only do we now have unparalleled access to genetic information, but we now have the tools to most accuartely understand what this genetic information – with genome editing allowing us to explore the genetic drivers of disease in physiological models.
AAV is a single-stranded, linear DNA virus with a a 4.7 kb genome which for the purpose of genome editing is replaced almost in entirety with the targeting vector sequence (except for the iTRs)
It is in effect a highly effective DNA delivery mechanism
After entry of the vector into the cell, target-specific homologous DNA is believed to activate and recruit HR-dependent repair factors can induce HR at rates approximately 1,000 times greater than plasmid based double stranded DNA vectors, but the mechanism by which it achieves this is still largely unknown
By including a selection cassette can select for cells that have integrated the targeting vector, and then screen for clones which have undergone targeted insetion rather than random integration, which will generally be around 1%.
Research Program Genetic Gains (RPGG) Review Meeting 2021: From Discovery to ...ICRISAT
A number of advances in genetics and genomics research of pigeonpea. These advances have enhanced our understanding of structural and functional aspects of genome and also provided us opportunities to deal with constraints impeding production of pigeonpea in precise and faster manner. Availability of the draft genome sequence and large-scale molecular markers has made it possible to map traits of interest in speedy manner. Although germplasm re-sequencing has already been started in pigeonpea, large-scale germplasm including elite breeding line, landraces and wild species is expected to be fully sequenced very soon.
Next generation genomics for chickpea (Cicer arietinum L.) improvementICRISAT
Large scale genomic resources including draft genome sequence, re-sequencing of 90 lines, comprehensive transcriptome assembly and high density genetic maps have been developed for chickpea. Linkage mapping and genome wide association studies (GWAS) are being used for trait
mapping.
26 Feb 2014
M. Bonierbale, E. Mihovilovich, W. Amoros, J. Landeo and M. Orrillo
Sustaining and Projecting Genetic Diversity for Potatoes Adapted To Changing Environments
14th Australasian Plant Breeding and 11th SABRAO Conference, Cairns, Queensland, Australia, 10-14 August 2009
ICRISAT Global Planning Meeting 2019:Research Program - Genetic Gains by Dr R...ICRISAT
The Global Planning Meeting 2019 focused on implementation plans for modernisation of ICRISAT crop improvement and to review and enhance the existing crop breeding programs, discuss modernization of crop improvement, and strategize how to harness new tools to maximize genetic gains. Innovation systems research was also discussed in detail to ascertain how all the different disciplines in crop improvement, innovation systems and other global and regional programs can work together to contribute to ICRISAT’s mission.
Science-based approaches for efficient conservation and use of genetic resourcesICARDA
11-14 February 2019. Jodhpur, India. The 13th International Conference on Dryland Development
13 February: Satellite Symposium Dryland Agrobiodiversity for Adaptation to Climate Change
Conservation and use of agrobiodiversity in CWANA drylands, Ahmed Amri (see picture), ICARDA, Morocco Head of ICARDA's Genetic Resources Unit, which runs the GRU seed bank in Rabat.
Towards fine mapping of drought tolerance related QTL region in chickpea usin...ICRISAT
Terminal drought is one of the major limitations to chickpea production, which results up to 50% of yield losses. Based on extensive genotyping and phenotyping data on ICC 4958 × ICC 1882 recombinant inbred line (RIL) population, a genomic region (~35cM) harbouring several QTLs for drought tolerance related traits was identified on linkage group 04 (LG 04). This region contained only four markers (TAA170, ICCM0249, STMS11 and GA24).
8 Jan 2013
Similar to TLM III: : Improve common bean productivity for marginal environments in sub-Saharan Africa - C Mukankusi (20)
Presentation made by the GCP Director during the CGIAR Fund Council (FC) visit to CIMMYT (GCP's host), on the sidelines of the FC meeting in Mexico in May 2014.
Presentation by the GCP Director at an international workshop on genomics and integrated breeding, February 2014. More on the workshop: http://bit.ly/MwpliD You can also view the presentation on video here: http://bit.ly/1mVmVdS
A quick introduction to the CGIAR Generation Challenge Programme (GCP) -- its history, network, research organisation, outputs and challenges. GCP is a virtual network of partnerships working on modern crop breeding for food security
Encryption in Microsoft 365 - ExpertsLive Netherlands 2024Albert Hoitingh
In this session I delve into the encryption technology used in Microsoft 365 and Microsoft Purview. Including the concepts of Customer Key and Double Key Encryption.
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
Neuro-symbolic is not enough, we need neuro-*semantic*Frank van Harmelen
Neuro-symbolic (NeSy) AI is on the rise. However, simply machine learning on just any symbolic structure is not sufficient to really harvest the gains of NeSy. These will only be gained when the symbolic structures have an actual semantics. I give an operational definition of semantics as “predictable inference”.
All of this illustrated with link prediction over knowledge graphs, but the argument is general.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Generating a custom Ruby SDK for your web service or Rails API using Smithyg2nightmarescribd
Have you ever wanted a Ruby client API to communicate with your web service? Smithy is a protocol-agnostic language for defining services and SDKs. Smithy Ruby is an implementation of Smithy that generates a Ruby SDK using a Smithy model. In this talk, we will explore Smithy and Smithy Ruby to learn how to generate custom feature-rich SDKs that can communicate with any web service, such as a Rails JSON API.
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
The Art of the Pitch: WordPress Relationships and SalesLaura Byrne
Clients don’t know what they don’t know. What web solutions are right for them? How does WordPress come into the picture? How do you make sure you understand scope and timeline? What do you do if sometime changes?
All these questions and more will be explored as we talk about matching clients’ needs with what your agency offers without pulling teeth or pulling your hair out. Practical tips, and strategies for successful relationship building that leads to closing the deal.
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
3. SNP genotpying:
Tm shift assay
A
G
from Wang et al. 2005°C
resistant
susceptible• Based on SNP-specific
primers
• Genotyping by melting
point analysis
• No fluorescent
primers required
4. C-A
BCMV: bc-3 candidate gene: eIF4E
NADERPOUR et al. MOLECULAR PLANT PATHOLOGY (2010)
primer 1
C
primer 2
A
primer reverse
9. Marker conversions
Chr Position G35346 AFR298 AND696 G10474 G40001 G5686 MD23-24 SEA5 VAX1
Chr01 219,278.00 T A A A A A A A A
Chr01 219,650.00 T C C C C C C C C
Chr01 219,722.00 T C C C C C C C C
Chr01 219,749.00 G A A A A A A A A
Chr01 219,950.00 C T T T T T T T T
Chr01 219,952.00 T A A A A A A A A
Chr01 220,668.00 T A A A A A A A A
Chr01 221,020.00 G C C C C C C C C
Chr01 252,386.00 C G G G G G G G G
Chr01 252,420.00 T A A A A A A A A
Chr01 253,519.00 A T T T T T T T T
Chr01 253,526.00 T G G G G G G G G
Chr01 253,542.00 C T T T T T T T T
Chr01 258,082.00 C G G G G G G G G
Chr01 264,339.00 C T T T T T T T T
Chr01 265,119.00 T G G G G G G G G
Chr01 265,168.00 T G G G G G G G G
Chr01 265,336.00 A G G G G G G G G
Chr01 265,418.00 C G G G G G G G G
Chr01 265,713.00 C T T T T T T T T
Chr01 265,722.00 C T T T T T T T T
Chr01 266,882.00 C A A A A A A A A
Chr01 267,398.00 A G G G G G G G G
Chr01 267,562.00 C A A A A A A A A
Chr01 267,688.00 T C C C C C C C C
Chr01 267,727.00 T A A A A A A A A
Chr01 268,418.00 C G G G G G G G G
Chr01 268,648.00 A C C C C C C C C
Chr01 271,111.00 A G G G G G G G G
Chr01 271,132.00 T C C C C C C C C
Chr01 271,437.00 A G G G G G G G G
Chr01 271,644.00 T C C C C C C C C
Chr01 272,223.00 G C C C C C C C C
Chr01 272,579.00 G A A A A A A A A
Chr01 272,645.00 G C C C C C C C C
Jorge et al
SNP sources:
• 1500 SNP set
BeanCAP
• 9 genotypes
sequenced
10. Angular Leaf Spot
Marker: Sc267437
Chr 8
G10474
Marker: P50
Chr. 4
G5686
• SNP based on SSR markers
confirmed and converted
• Finemapping on Chr 4
– Resistance gene 43.5 – 43.9 Mbp
42.0 42.5 43.0 43.5 44.0 44.5 45
0
2
4
6
8
Chromosome 4 (Mbp)
LODscore
Beat Keller, 2013
11. Bean Stem Maggot
• QTL mapping underway
• 100 additional SNPs genotyped (Daniel Ambachew)
• BSM evaluations in Zimbabwe, Malawi and Ethiopia
tolerant susceptible
Maps for DOR x BAT
SER16 x G35346
13. MARS
• PhD thesis of Fitsum Alemayehu SARI,
Ethiopia
• Population of ~200 Lines
• Phenotyping drought & irrigated at 4
locations
– 2x Palmira, 2x Ethiopia
• Genotyping:
– Kbio/LGC ~150 SNP markers
– additional 14.000 fluidigmdata points
• Marker Analysis in progress
– DNAs sent, extracted from seed
14. (CAL 143xSAB 620) X SAB 626 (SAB 628xCAL 143) X SAB 659 (SAB 628xCAL 143) X SAB 686
G 12229 x AND 277
CAL143
(ABA 58x AFR298)F1xSAB258(COS 16 x AFR298)F1 x SAB258
SAB258 ABA58AFR298COS16
41 lines 54 lines 30 lines
26 lines 42 lines
F5
F5F1: CAL 143xSAB 620
F1: SAB 628xCAL 143
F1: ABA 58x AFR298
F1: COS 16 x AFR298F1F1F1
F1
F1
SAB620 SAB626 SAB628 SAB659 SAB686
MARS
15. MAGIC
• 8-parental population established
• F4:6 Phenotyping started in July
• Genotyping: DNA ready to send for GBS
16. SXB 412 INB 827 ALB 213 SEN 56 SCR 2 MIB 778 SCR 9 INB 841
79 F1 5 F1 38 F1 99 F1
323 F1
272 F1
728 F1
500 selected
499 F2
996 F3 families
926 F4.5 families
926 F4:6 families
MAGIC population in common bean
(mesoamerican gene pool)
Genotype individuals
Drought phenotyping
18. Breeding products
Relationship between irrigated and rainfed grain yield of 36 common bean
genotypes grown under field conditions at CIAT-Colombia (2013)
AFR298
CAL143
CAL96
DAB 231
DAB 233
DAB 236
DAB 244
DAB 251
DAB 252
DAB 256
DAB 258
DAB 267
DAB 277
DAB 295
DAB 344
DAB 353
DAB 366
DAB 374DAB 380
DAB 384
DAB 396
DAB 398
DAB 402
DAB 441DAB 489 DAB 494 DAB 514
DAB 520 DAB 525
DAB 528
DAB 534
DAB 541
DAB 545
DAB 549
DAB 555
KATB1
0
200
400
600
800
1000
1200
2000 2500 3000 3500 4000 4500
droughtYDHA
irrigated YPDA
2013 YDHA
19. Data management
• Adopting defined file formats
• Adopting barcoding
• Adopting use of barcoding for seed storage
organization
• Training of partners to adopt defined file
formats
Sheet 1 Sheet 2
Trial data
Controlled vocabulary
trait dictionary
Metadata
• Sowing date
• Location
• Person in charge
20. Capacity building
• Workshop with 30+ breeders in
Kawanda
• 2 workshops at CIAT (n ~20)
• Fitsum Alemayehu (phd student,
Ethiopia) visited CIAT for 3 months.
21. Conclusions
• MAS for biotic stress moving toward routine
• MAGIC and MARS moving forward
• Advanced lines show improved yield under
drought
• Data Management moving towards adoption
• Capacity building ongoing
Anticipated impact: improved germplasm …
22. View to the future
• Unite TL 1 and TL2 activities
• Technology changes
– GBS: for fingerprinting, population genotyping
– genomic selection
• Fingerprinting / Forensics
– Specific projects need to be defined
– Impact studies
– software
• Organizational changes
– Extend work with partners
– Data management -software
– Links to
• Soils work, agronomy
• Other downstream activities
23. Thank you for your attention!
SARI Ethiopia
SARI SELIAN Tanzania
DARS Malawi
DR&SS Zimbabwe
KARI Kenya
CIAT Africa