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Cowpea Product Delivery
Ousmane Boukar
IITA, Ibadan, Nigeria
Outline
Product Catalogue Summary
GCP Transition Checklist
• Not Finished by Project End
• To Be delivered by Project End
• Recently Finished, Ready for Catalog
Product Catalogue Summary – Cowpea
Informative markers available
• Fusarium resistance
• Drought tolerance (staygreen, yield, biomass)
• Maturity
• Striga resistance
• Root-knot nematode resistance
• Flower and foliar thrips resistance
• Bacterial blight resistance
• Macrophomina (ashy stem blight) resistance
Genomic resources
• Physical map (BACs)
• Microarray chip
• SSR marker kit
• Consensus genetic map (~1100 SNPs, 11 RILs)
• Genetic map (527 SNPs, 1 RIL)
Product Catalog Summary – Cowpea
Informatics/Application Tools
• HarvEST:Cowpea (Windows browser)
• BreedIt SNP Selector (input parents, cM, trait positions)
• ParentChecker (to infer RIL parent genotypes)
• KBio Converter (to change reference strand)
Germplasm
• Reference set (370 accessions)
• 20 drought tolerant reference lines for West Africa
GCP Transition Checklist – TL1
Activity 1: Develop MAGIC population
Activity 2: Develop genomic resources in support of
marker-assisted breeding
Activity 3: Employ MARS and MABC to develop
improved breeding lines
Activity 4: Capacity Building
Activity 5: Curate and Store Phase I and II Data
GCP Transition Checklist
Activity 1: Develop MAGIC population
• By December 2015, need funds to advance and bulk F8
Activity 3: Employ MARS and MABC to develop
improved breeding lines
• MARS, by December 2015, need funds for phenotyping
(two years, 4 populations, 1 per location)
• MABC, by December 2015, need funds for phenotyping
(two years, 8 populations, 2 per location)
Not Finished by Project End (2014)
GCP Transition Checklist
Activity 2: Develop genomic resources in support of
marker-assisted breeding
• RIL population seed and SNP data
Activity 3: Employ MARS and MABC to develop
improved breeding lines
• QTL for aphid resistance published
To be Delivered by Project End (2014)
GCP Transition Checklist
Activity 4: Capacity Building
• Training African students and breeders
Activity 5: Curate and Store Phase I and II Data
• Finish formatting and delivering phenotype and genotype
datasets
To be delivered by project end (2014)
GCP Transition Checklist
Activity 2: Develop genomic resources in support of
marker-assisted breeding
• ~1000 mapped KASP SNPs
• Updated consensus map (11 RILs)
• List of 100-400 customized SNP markers developed for 4
MARS and 8 MABC breeding populations
• SNP marker data used for MARS and MABC breeding
decisions
Recently finished, ready for catalog
GCP Transition Checklist
Activity 3: Employ MARS and MABC to develop
improved breeding lines
• Markers for 5 heat tolerance QTL
Recently finished, ready for catalog
Thank You

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GRM 2013: Cowpea product catalogue and project status -- Projects ongoing, completed, and to be continued post-2014 -- O Boukar

  • 1. Cowpea Product Delivery Ousmane Boukar IITA, Ibadan, Nigeria
  • 2. Outline Product Catalogue Summary GCP Transition Checklist • Not Finished by Project End • To Be delivered by Project End • Recently Finished, Ready for Catalog
  • 3. Product Catalogue Summary – Cowpea Informative markers available • Fusarium resistance • Drought tolerance (staygreen, yield, biomass) • Maturity • Striga resistance • Root-knot nematode resistance • Flower and foliar thrips resistance • Bacterial blight resistance • Macrophomina (ashy stem blight) resistance Genomic resources • Physical map (BACs) • Microarray chip • SSR marker kit • Consensus genetic map (~1100 SNPs, 11 RILs) • Genetic map (527 SNPs, 1 RIL)
  • 4. Product Catalog Summary – Cowpea Informatics/Application Tools • HarvEST:Cowpea (Windows browser) • BreedIt SNP Selector (input parents, cM, trait positions) • ParentChecker (to infer RIL parent genotypes) • KBio Converter (to change reference strand) Germplasm • Reference set (370 accessions) • 20 drought tolerant reference lines for West Africa
  • 5. GCP Transition Checklist – TL1 Activity 1: Develop MAGIC population Activity 2: Develop genomic resources in support of marker-assisted breeding Activity 3: Employ MARS and MABC to develop improved breeding lines Activity 4: Capacity Building Activity 5: Curate and Store Phase I and II Data
  • 6. GCP Transition Checklist Activity 1: Develop MAGIC population • By December 2015, need funds to advance and bulk F8 Activity 3: Employ MARS and MABC to develop improved breeding lines • MARS, by December 2015, need funds for phenotyping (two years, 4 populations, 1 per location) • MABC, by December 2015, need funds for phenotyping (two years, 8 populations, 2 per location) Not Finished by Project End (2014)
  • 7. GCP Transition Checklist Activity 2: Develop genomic resources in support of marker-assisted breeding • RIL population seed and SNP data Activity 3: Employ MARS and MABC to develop improved breeding lines • QTL for aphid resistance published To be Delivered by Project End (2014)
  • 8. GCP Transition Checklist Activity 4: Capacity Building • Training African students and breeders Activity 5: Curate and Store Phase I and II Data • Finish formatting and delivering phenotype and genotype datasets To be delivered by project end (2014)
  • 9. GCP Transition Checklist Activity 2: Develop genomic resources in support of marker-assisted breeding • ~1000 mapped KASP SNPs • Updated consensus map (11 RILs) • List of 100-400 customized SNP markers developed for 4 MARS and 8 MABC breeding populations • SNP marker data used for MARS and MABC breeding decisions Recently finished, ready for catalog
  • 10. GCP Transition Checklist Activity 3: Employ MARS and MABC to develop improved breeding lines • Markers for 5 heat tolerance QTL Recently finished, ready for catalog

Editor's Notes

  1. I’ll be reviewing Year 1 progress on the 4 key project activities, viz…
  2. I’ll be reviewing Year 1 progress on the 4 key project activities, viz…
  3. I’ll be reviewing Year 1 progress on the 4 key project activities, viz…
  4. I’ll be reviewing Year 1 progress on the 4 key project activities, viz…