This document describes recto-vaginal fistula in female calves. It is a congenital condition where the rectum and vagina directly communicate, allowing for urofecal mixing. It is accompanied by atresia ani, where the rectal pouch ends before the anus. Treatment involves separating fused vulvular lips if present, identifying and closing the fistula, surgically correcting the atresia ani by creating an anus, and providing postoperative care including antibiotics and ointment.