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RAMAN
SPECTROSCOPY
Presented by​
Md Ariful Islam
M. Pharm 2nd Semester
Department of Pharmaceutical Sciences
Dibrugarh University
Advanced Spectral Analysis(MPC 201T)
Principle, instrumentation and applications
CONTENT
Introduction​
Principle of Raman Spectroscopy
​Instrumentation
Applications
​Summary
References​
INTRODUCTION
Milestones of Raman spectroscopy
• 1923 : Inelastic light scattering predicted by A. Smekal
• 1928 : C.V. Raman and K.S. Krishnan see "secondary radiation"
from neat solvents (Nobel Prize 1930)
• 1960 : Invention of laser - Breakthrough for Raman experiments
3
4
PRINCIPLE
Raman Spectroscopy
6
Interaction of light with a sample
Laser light (monochromatic) interacts with sample
Laser light can be:
• Absorbed
• Transmitted
• Scattered
• →Elastic scattering / Rayleigh (green)
• →Inelastic scattering / Raman (red and blue
7
8
9
10
11
INSTRUMENTATION
Raman Spectroscopy
13
14
APPLICATION
Raman Spectroscopy
APPLICATION
Raman Spectroscopy
APPLICATION
Raman Spectroscopy
APPLICATION
Raman Spectroscopy
APPLICATION
Raman Spectroscopy
20
APPLICATION
Raman Spectroscopy
SUMMARY 22
• Raman spectroscopy analyzes the interaction of light with matter to gather
information about molecular structure and composition.
• It is based on the Raman scattering phenomenon discovered by Sir C.V. Raman.
• Monochromatic light is directed onto a sample, and the scattered light is analyzed.
• The majority of scattered light undergoes elastic scattering (Rayleigh scattering),
while a small fraction undergoes inelastic scattering (Raman effect).
• Inelastic scattering occurs due to interactions with molecular vibrations and
rotations.
• The energy shifts in the scattered light provide insights into the chemical bonds,
molecular structure, and composition of the sample.
• A Raman spectrum is obtained by analyzing the energy shifts in the scattered light.
• Raman spectroscopy is widely used in chemistry, materials science,
pharmaceuticals, forensics, and biology for qualitative and quantitative analysis.
23
"The Raman effect has opened up new vistas
in the field of molecular spectroscopy,
providing an invaluable tool for the study of
molecular structure and dynamics."
Quotes
REFERENCES
24
1. C.V. Raman & K. S. Krishnan, A New Type of Secondary Radiation, Nature 121, 501- 502 (1928)
2. Smith & G. Dent, Modern Raman Spectroscopy: A Practical Approach 1st ed. , Wiley (2005
THANK YOU

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Raman spectroscopy.pptx

  • 1. RAMAN SPECTROSCOPY Presented by​ Md Ariful Islam M. Pharm 2nd Semester Department of Pharmaceutical Sciences Dibrugarh University Advanced Spectral Analysis(MPC 201T) Principle, instrumentation and applications
  • 2. CONTENT Introduction​ Principle of Raman Spectroscopy ​Instrumentation Applications ​Summary References​
  • 3. INTRODUCTION Milestones of Raman spectroscopy • 1923 : Inelastic light scattering predicted by A. Smekal • 1928 : C.V. Raman and K.S. Krishnan see "secondary radiation" from neat solvents (Nobel Prize 1930) • 1960 : Invention of laser - Breakthrough for Raman experiments 3
  • 4. 4
  • 6. 6 Interaction of light with a sample Laser light (monochromatic) interacts with sample Laser light can be: • Absorbed • Transmitted • Scattered • →Elastic scattering / Rayleigh (green) • →Inelastic scattering / Raman (red and blue
  • 7. 7
  • 8. 8
  • 9. 9
  • 10. 10
  • 11. 11
  • 13. 13
  • 14. 14
  • 20. 20
  • 22. SUMMARY 22 • Raman spectroscopy analyzes the interaction of light with matter to gather information about molecular structure and composition. • It is based on the Raman scattering phenomenon discovered by Sir C.V. Raman. • Monochromatic light is directed onto a sample, and the scattered light is analyzed. • The majority of scattered light undergoes elastic scattering (Rayleigh scattering), while a small fraction undergoes inelastic scattering (Raman effect). • Inelastic scattering occurs due to interactions with molecular vibrations and rotations. • The energy shifts in the scattered light provide insights into the chemical bonds, molecular structure, and composition of the sample. • A Raman spectrum is obtained by analyzing the energy shifts in the scattered light. • Raman spectroscopy is widely used in chemistry, materials science, pharmaceuticals, forensics, and biology for qualitative and quantitative analysis.
  • 23. 23 "The Raman effect has opened up new vistas in the field of molecular spectroscopy, providing an invaluable tool for the study of molecular structure and dynamics." Quotes
  • 24. REFERENCES 24 1. C.V. Raman & K. S. Krishnan, A New Type of Secondary Radiation, Nature 121, 501- 502 (1928) 2. Smith & G. Dent, Modern Raman Spectroscopy: A Practical Approach 1st ed. , Wiley (2005