Vitamins A and E are fat-soluble vitamins that play important roles in vision, antioxidant activity, and other metabolic functions. Vitamin A is found in foods like carrots, green vegetables and fruits, and helps with night vision. Vitamin E sources include nuts, seeds, and vegetable oils, and it acts as an antioxidant to repair tissues. Both vitamins can be quantified using chromatographic methods by measuring absorption peaks - vitamin A is measured using UV spectrophotometry while vitamin E uses gas chromatography to separate and measure peaks from standards.
Carbohydrates || Food Analysis || Pharmaceutical Analysis Department || M.Pha...saimuniswetha1
Hello everyone,
Today's topic Carbohydrates in Food Analysis subject in M.pharmacy(Pharmaceutical Analysis Department) ..Don't forget to see.. please watch it... If you need explanation about Carbohydrates please click below link : https://youtu.be/aI5UnNYgufY
In this slide I have given brief knowledge about types of preservatives. This slide is recommended to students who are new to this particular topic or those who want notes for examination. I hope you will get benefit from this slide. Do comment for any improvement or want slides that i should prepare for you.
Determination of vitamin a in the supplied sample.Atai Rabby
Vitamin A is assayed from the biological samples by high performance liquid chromatography(HPLC) method after processing and separation of samples, as the method is specific, accurate and sensitive but it is also widely measured by calorimetrically for the last many years using the maximum intensity of color developed at 620 nm by the method of Carr and price.
method for crude protein anylsis (Kjeldahl).pptxAmita Beniwal
This method was specifically developed by Danish
chemist, Johan G.C.T. Kjeldahl, in 1883 to determine
the nitrogen contents in organic and inorganic
substances (like foodstuffs, fertilizers, wastewater,
soil, feed, grain, and other substances).
⦿ This method is also used for estimating the crude
protein content in food.
As per the syllabus prescribed by Rajiv Gandhi University of Health Sciences, Karnataka, for M. Pharm (Pharmaceutical Analysis), 1st semester.
*not all topics have been covered in this file.
Lipids : Classification & general analysisVinit Gohel
brief knowledge about lipid, its classification and method of analysis are covered in this slide. Students searching for notes can refer this slide. Hope it will help you.
Carbohydrates || Food Analysis || Pharmaceutical Analysis Department || M.Pha...saimuniswetha1
Hello everyone,
Today's topic Carbohydrates in Food Analysis subject in M.pharmacy(Pharmaceutical Analysis Department) ..Don't forget to see.. please watch it... If you need explanation about Carbohydrates please click below link : https://youtu.be/aI5UnNYgufY
In this slide I have given brief knowledge about types of preservatives. This slide is recommended to students who are new to this particular topic or those who want notes for examination. I hope you will get benefit from this slide. Do comment for any improvement or want slides that i should prepare for you.
Determination of vitamin a in the supplied sample.Atai Rabby
Vitamin A is assayed from the biological samples by high performance liquid chromatography(HPLC) method after processing and separation of samples, as the method is specific, accurate and sensitive but it is also widely measured by calorimetrically for the last many years using the maximum intensity of color developed at 620 nm by the method of Carr and price.
method for crude protein anylsis (Kjeldahl).pptxAmita Beniwal
This method was specifically developed by Danish
chemist, Johan G.C.T. Kjeldahl, in 1883 to determine
the nitrogen contents in organic and inorganic
substances (like foodstuffs, fertilizers, wastewater,
soil, feed, grain, and other substances).
⦿ This method is also used for estimating the crude
protein content in food.
As per the syllabus prescribed by Rajiv Gandhi University of Health Sciences, Karnataka, for M. Pharm (Pharmaceutical Analysis), 1st semester.
*not all topics have been covered in this file.
Lipids : Classification & general analysisVinit Gohel
brief knowledge about lipid, its classification and method of analysis are covered in this slide. Students searching for notes can refer this slide. Hope it will help you.
Aging is a natural part of human life. However, recent discoveries indicate that pharmacological approaches used for the improvement and possibly, for the delay of the aging process, might shed a new light on this topic. This might obviously contribute to the extension of the active life of older people and maintenance of their quality of life, which could consequently reduce both social and economic burden of each country, especially the developed ones.
Vitamin A, Digestion, absorption, transport, Functions and requirement and deficiency ad eye relate problems.
Vitamin C, Functions, requiremnts, deficiency
Vitamin E, defciency and eye
It is a slide to teach students in universities about the basics of vitamin A, its benefits, metabolism, clinical indication, and also general information.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Palestine last event orientationfvgnh .pptxRaedMohamed3
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Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
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Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
2. VITAMINS
Vitamins are naturally occurring organic compounds
that are essential to metabolic or other functions in the
body.
Most vitamins cannot be synthesized by the body.
They must be supplied in the diet.
Vitamins are usually classified as water soluble or fat
soluble
3. CLASSIFICATION OF VITAMINS
Vitamins can be classified as either
• Water soluble
• Fat soluble.
Water soluble vitamins are generally involved in
the cellular metabolism of energy supplying
nutrients.
Fat soluble vitamins often have very specialized
functions
6. Vitamin A Sources
Commonly found in cod liver oil, green vegetables,
and fruits.
Carrots indirectly serve as a source of vitamin A
since they contain β carotene which the body
readily converts to vitamin A
7. Vitamin A Functions
Vitamin A is fat soluble.
It is not readily broken down by cooking.
Role in aiding in night vision.
Vitamin A Deficiencies
A deficiency in vitamin A results in night blindness.
The most serious deficiency results in a condition
known as Xeropthalmia, a severe form of
conjunctivitis or blindness.
8. Vitamin E sources
vitamin E can be found most abundantly in wheat
germ oil, sunflower, and safflower oils and also in nuts,
cereals, olive, tomato, spinach, blue crab.
olive nuts cereals
9. Vitamin E functions
Vitamin E is used to refer to a group of fat-soluble
compounds that include both tocopherols and
tocotrienols.
α-Tocopherol is an important lipid-soluble
antioxidant.
α-Tocopherol has a regulatory effect on enzymatic
activities
responsible for the repair of the wounds and
regeneration of the extracellular tissue, vitamin E also
plays a role in neurological functions, and inhibition of
platelet aggregation
10. Quantitative determination of
vitamin A
Category: antixerophthalmic vitamin
Structure:
Standards: not less than 95% and not more than 110%
of standard number of units of vitamin A per g.
11. Identification: exhibits a maximum at about 325-
327nm.
QUANTITATIVE ESTIMATION METHOD:
UV-Spectrophotometric method
principle:
it absorbs the UV-radiation ranges
from 200nm to 400nm.
Valence electrons absorb the energy, there by molecule
undergo transition from ground state to excited state.
By the absorption peaks the nature of electrons and
molecular structure can be elucidated.
12. METHOD:
Dissolve an accurately weighed quantity of the
substance in cyclohexane to give a solution containing
9 to 15 units of vit A per ml.
Determine the wave length of maximum absorption.
Measure the absorbance of the solution against cyclo
hexane at 328 nm.
If the wave length of maximum absorption lies
between 326 and 329 calculate the vit A potency of the
sample from the expression
A328(A1%
1cm) × 1900 = vit A potency in units per g.
13. If the relative absorbances are not within the limits
then calculate a corrected absorbance at about 328nm
by applying the observed values to the equation
A328(corr.) = 3.52 (2A328 –A316 – A 340)
If the wavelength of maximum absorbance lies out
side the range of 323 to 327 then the unsaponifiable
fraction of the sample must be further purified by
chromatography.
14. Quantitative determination of
vitamin E
Category: anti oxidant
Structure:
Standards: it contains not less than 96% and not more
than 102% of C31H52O3.
15. QUANTITATIVE ESTIMATION METHOD:
Chromatographic method:
Principle:
The principle of separation in GAS
CHROMATOGRAPHY is partition.
They travel according to their partition coefficients
towards the stationary phase.
More soluble travels slower
Less soluble travels faster
No two components have same partition coefficients.
The components are separated according to their
partition coefficients.
16. METHOD:
Internal standard solution: dissolve an accurately
weighed quantity of hexa decyl hexa decanoate in n-
hexane to obtain a solution having a known
concentration of about 1mg per ml.
Standard preparation: dissolve in internal standard
solution a suitable quantity of USP alpha tocopherol
RS to obtain a solution having known concentration of
1mg per ml.
Assay preparation: transfer 50mg of vitamin E to a
50ml volumetric flask, dissolve in internal standard
solution, dilute with internal standard solution to
volume and mix.
17. Chromatographic system
The GC instrument is
equipped with a flame
ionization detector.
It contains 2m-4mm
borosilicate glass column
packed with 2% to 5%
liquid phase on 80 to 100
mesh support utilizing a
column injection.
The column is
maintained at a temp.
between 245° to 265°
18. The injection port and detector block are maintained at
about 10° higher than the column temperature.
The flow rate of dry carrier gas is adjusted to obtain a peak
approximately 18 to 20 min after sample introduction.
Procedure:
Inject a suitable portion (2-5µl) of the assay preparation
into a suitable gas chromatograph.
Record the chromatogram .
Measure the areas under the 1st(alpha tocopherol) and 2nd
major(hexadecyl hexa decanoate)peaks, record the values
as aU and aD respectively.
Calculate the quantity in mg, of vit E taken by the formula
(50CD/F)(aU/ aD)
Where CD is conc.in mg/ml
F is the relative response factor
19. REFERENCES
INDIAN PHARMACOPOEIA
BRITISH PHARMACOPOEIA
UNITED STATES PHARMACOPOEIA
www.googleimages.com
www.wikipedia.com
www.cyberlipid.org