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PREPARATION-OF-MICROBIOLOGICAL-MEDIA.pdf
- 1. CREDITS: This presentation template was created by Slidesgo, including icons by Flaticon, and infographics & images by Freepik Preparation of Microbiological Media BIOTECHNOLOGY
- 2. What is Microbiological Media? Culture media is a gel or liquid that contains nutrients and is used to grow bacteria or microorganisms. It contains everything bacteria need to grow outside the body and under laboratory conditions. When a particular bacterium needs to be cultivated in order to verify the presence of an infection or further investigate a particular bacterium, bacterial culture media are used.
- 3. MEDIA CLASSIFIED ACCORDING TO: MEDIA COMPOSITION SELECTIVITY CONSISTENCY
- 4. • Media classification according to Composition can either be empirical or natural and synthetic. • Consistency- Media are classified as liquid, solid, and semi-solid. • Selectivity- media are categorized as General, nonselective or selective and Differential.
- 5. • Liquid Media- such as nutrient broths can be used to propagate large number of microorganisms in fermentation studies and various biochemical tests. • Solid Media- created by adding gelatin and/or agar (such as nutritional agar) to the liquid media to act as hardening agents. Classification of culture media based on consistency
- 6. Semi-solid Media- can be used in determining bacterial motility and promoting anaerobic growth.
- 7. Materials/Tools ELECTRONIC WEIGHING BALANCE Erlenmeyer Flask Agar Teste DNase Aluminum Foil Petri-dish AUTOCLAVE Laboratory Incubator Graduated Cylinder
- 8. Glass syringe Cotton plug Beaker Autoclave plastic bag Test tube
- 10. STEP 1: CALCULATE THE PRECISE AMOUNT OF MEDIUM NEEDED. MEDIA IN PLATES
- 11. STEP 2 WEIGH THE TOTAL AMOUNT OF POWDERED MEDIUM CALCULATED USING FOIL AT ITS APPROPRIATE SIZE TO HOLD THE MEDIUM.
- 12. STEP 3 PUT THE USED POWDER(agar) AND TRANSFER ON THE ALUMINUM FOIL USING DRIED SPATULA AND ENOUGH WEIGHT TO ONE YOU COMPUTED.
- 13. STEP 4 CAREFULLY TRANSFER POWDER INTO A FLASK OR BEAKER AND WASH OFF ANY POWDER ON THE FLASK AND MIX WITH EVERY ADDITION AFTER IT.
- 14. STEP 5 ADD DISTILLED WATER ON THE FLASK AND MIX WITH EVERY ADDITION AFTER IT.
- 15. STEP 6 TRANSFER INTO A FLASK, THEN SEAL WTH COTTON PLUG AND SOME FOIL PLACE IN A AUTOCLAVABLE PLASTIC BAG AND STERILIZE USING AN AUTOCLAVE DO NOT FORGET FOIL.
- 16. What is the purpose of autoclaving the culture media? Autoclaves use high pressure and heat to kill spores and germs. They are used to sterilize lab equipment, sterilize media, and decontaminate specific biological waste.
- 17. STEP 7 MAKE SURE TO ALLOW THE MEDIUM TO COOL INSIDE A BIOSAFETY CABINET AT 48 TO 50 DEGREES CELCIUS.
- 18. STEP 8 Aseptically pour a medium into the plates and fill only 1/3 of the height of the plate and gently swirl the plate side to side.
- 19. STEP 9 Invert the plates and store them in the incubate for 24 hours before using it.
- 21. S1- Calculate the total amount of medium needed. S2- Prepare a weighing box or a piece of foil. large enough to hold the powdered medium S3- Transfer powdered media into the weighing container until desired weight is obtained S4- Transfer powder into a flask or beaker, or immediately cover the weighing container and then label for later use. S5- Add the required amount of Distilled water S6- Melt the agar in an microwave oven until liquid is clear and homogeneous
- 22. S7- Dispense about 8ml of broth into screwcap tubes S8- Place the tubes in a beaker or test tube rack and cover with aluminum foil. Place the beaker with the tubes in an Autoclavable plastic bag. S9- Sterilize in an autoclaves immediately S10- To make slant, lay the tubes containing the agar on a slanting board. Allowed to solidify and cool. Let the broth tube cool completely. S11- Label and Refrigerate both slants and broth for future use.
- 23. video