Success rates for IVF are only around 20% due to chromosomal abnormalities in embryos that often go undetected during conventional embryo selection methods. Chromosomal issues like aneuploidy, where there is an atypical number of chromosomes, can lead to embryo death, miscarriage, or health problems in babies. Preimplantation genetic diagnosis (PGD) involves testing embryos for chromosomal or genetic disorders before transferring to the womb, improving IVF success rates and preventing transmission of genetic disorders. PGD is recommended for advanced maternal age, repeated miscarriages or IVF failures, hereditary disorders, and translocation carriers.
PGD is a state-of-the-art procedure used in conjunction with In Vitro Fertilization (IVF) in which the embryo is tested for certain conditions prior to being placed in the womb of the woman.
PGD combines advances in Molecular genetics and in assisted reproductive technology and is conducted before the embryo is placed inside the womb of the woman.Pre implantation genetic diagnosis was introduced to prevent the inheritance of sex linked diseases
PGD is a state-of-the-art procedure used in conjunction with In Vitro Fertilization (IVF) in which the embryo is tested for certain conditions prior to being placed in the womb of the woman.
PGD combines advances in Molecular genetics and in assisted reproductive technology and is conducted before the embryo is placed inside the womb of the woman.Pre implantation genetic diagnosis was introduced to prevent the inheritance of sex linked diseases
Preimplantation Genetic Diagnosis (PGD)/Screening (PGS) With IVFKaberi Banerjee
Pre-implantation genetic Screening (PGS) or Pre-implantation genetic diagnosis (PGD) plays an important role in increasing the chances of pregnancy for infertile couples.
Preimplantation genetic diagnosis (PGD) is a procedure used to diagnose embryos for known genetic disorders that both the patients and partners.
Read more: https://www.advancefertility.in/preimplantation-genetic-diagnosis-pgd-pgs-with-ivf/
Lecture on prenatal genetic diagnostic techniques and their value in detection of prenatal genetic anomalies. This lecture details techniques employed in the common diagnostic interventions used in prenatal period and their usefulness.
interest in stem cells is raising in different field of medicine. The question is : is it successful in Gynecology or it is still too early to say that. The present talk may help to explore this .
EMBRYO QUALITY ASSESSMENT, WHICH TO SELECT? Rahul Sen
Traditional embryo evaluation systems are simple, non-invasive, cost-effective & mainstay in majority of IVF laboratories. Embryo selection based on combinations of morphology scores at different stages of embryonic development with time may be more effective
Preimplantation Genetic Diagnosis (PGD)/Screening (PGS) With IVFKaberi Banerjee
Pre-implantation genetic Screening (PGS) or Pre-implantation genetic diagnosis (PGD) plays an important role in increasing the chances of pregnancy for infertile couples.
Preimplantation genetic diagnosis (PGD) is a procedure used to diagnose embryos for known genetic disorders that both the patients and partners.
Read more: https://www.advancefertility.in/preimplantation-genetic-diagnosis-pgd-pgs-with-ivf/
Lecture on prenatal genetic diagnostic techniques and their value in detection of prenatal genetic anomalies. This lecture details techniques employed in the common diagnostic interventions used in prenatal period and their usefulness.
interest in stem cells is raising in different field of medicine. The question is : is it successful in Gynecology or it is still too early to say that. The present talk may help to explore this .
EMBRYO QUALITY ASSESSMENT, WHICH TO SELECT? Rahul Sen
Traditional embryo evaluation systems are simple, non-invasive, cost-effective & mainstay in majority of IVF laboratories. Embryo selection based on combinations of morphology scores at different stages of embryonic development with time may be more effective
“Difficulty encountered in the delivery of the fetal shoulders after delivery of the head.”
Shoulder dystocia is an unpredictable obstetric complication with the incidence of 0.15% to 2%.
An increase in the incidence of shoulder dystocia has been recorded over the last 20 years. Incidence appears to be increasing as birth weights increase.
Amniotic fluid maintain the perfect homeostasis between mother and fetus. It protect both mother and fetus from various complications. Details is enclosed in presentation.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
4. Chromosomal abnormalities often
neglected at the time of
embryo selection
Conventional methods of embryo selection
• Embryoscopy
• Physical identification
• Morphological characteristics
5. • Aneuploidy: atypical number of chromosomes results from
error in cell division
• Translocations: a piece of chromosome go and attached to a
wrong chromosome.
• Chromosome Deletions : loss of a chromosome segment
resulting in an imbalance in the number of genes present
• Chromosome inversions: when a single chromosome breaks
in two places and the material is reconstituted upside down
……leading to embryo death, miscarriage or live birth of an infant
with substantial medical problems.
6. • A healthy baby has 46 chromosomes in each cell of
the body
• Chromosomal abnormality refers to an atypical
number of chromosomes and is generally caused by
an error in cell division in either the egg or sperm or
in the first few days of embryo development.
7. Normal cell
Meiosis
Embryos with chromosomal abnormalities are less likely
to implant and more likely to end in miscarriage
8. Age in Normal Aneuploidy Other
years embryos (%) embryos (%) abnormalities (%)
25-35 61 8 31
36-37 60 10 30
38-39 47 18 35
40-41 43 26 31
42-44 39 30 31
http://www.fertilityindia.com/indications-for-pgd.php
9. • Decreased embryo transfer
• Implantation failure,
• Recurrent pregnancy loss, or miscarriage
• Birth defects
• Mental or physical problems in offspring
10. Babies born with chromosome abnormalities
have mental retardation and / or birth defects
Ex: Down syndrome
12. • Age more than 35 years (chances of embryo transfer
(<75%)
• Couples with a family history of X-linked disorders
(25% risk of having an affected embryo)
• Carriers of autosomal recessive diseases: means
two copies of an abnormal gene (the risk an embryo
may be affected is 25%.)
• Carriers of autosomal dominant diseases: abnormal
gene in one parent (the risk an embryo may be
affected is 50%.)
13. Typical scenario
Carrier mother + Normal father
25% chances 25% chances 25% chances 25% chances
of Normal boy of disease boy of Normal girl of carrier girl
Disease father + Normal mother
100% chances of 100% chances of
Normal boy carrier girl
Carrier: Some one has one abnormal gene (No symptoms) is called a carrier
14. Women with X-linked recessive disorder are rare, since women has two X-chromosomes.
Two scenarios:
Carrier mother + Disease father
25% chances 25% chances 25% chances 25% chances
of Normal boy of disease boy of carrier girl of disease girl
Disease father + Disease mother
100% chances disease boy or girl
http://www.fertilityindia.com/indications-for-pgd.php
15.
16. Egg Sperm
Embryo 8 cell stage
Remove one cell
On day 3rd
Test DNA or Test results
chromosomes
Healthy gen Unhealthy gen
conditions conditions
Embryo implanted Embryo
on day 4 discarded
17. • Couples with advanced maternal age (>35)
• Unexplained infertility
• Y-chromosome deletion: found in 5-20% men with low sperm
count
• Couples who have experienced repeated miscarriages
• Couples who have experienced repeated IVF failures
• Couples who have previously had a pregnancy with a
chromosomal abnormality
• Couples at risk of having children with a particular X-linked
disorder
• couples where one partner carries a balanced chromosomal
translocations
18. • In only few centers it is available in India
• Most well known center is Jaslok Hospital, Mumbai
• It takes 3 days
• It is limited to chromosomes 13, 18, 21 only
19. • PGD is highly accurate across all chromosomes
• It yields quick results which allows for a fresh embryo
transfer on day 4 when it is tested on day 3
• PGD prevents transmission of genetic disorders onto
future generations.
20.
21. • There are two main types of preimplantation
genetic screening
– Aneuploidy screening by fluorescent in situ
hybridization (FISH) and
– Aneuploidy screening by Molecular Array
Comparative Genomic Hybridization (CGH-24)
22. • It screens the most common chromosomes seen in
miscarriages and live born abnormality disorders like
Down syndrome-chromosomes 13, 16, 18, 21 and 22.
• When one parent is carrier of heritable X-linked disease
such as Muscular dystrophy, Hemophilia A, or
ectodermal Dysplasia.
• FISH examines chromosomes X and Y plus chromosomes
13, 18, and 21
23. • This is single cell technology to test
aneuploidy, translocations, inversion and chromosomal
abnormality
• It is unique and it can make thousands of independent
measurements of each chromosome at molecular level
• No freezing and ICISI is required
• It is automated and high degree of results accuracy
• It gives the results with 20 hours than the standard 36-48
hours
24. • On day 3rd: Biopsy of a blastomere from an embryo
at IVF center
– The fixation of blastomere on slides for FISH analysis or
transfer into PCR tube with lysis buffer for CGH analysis
– Test for FISH or CGH at genetic center : results within 12-20
hours
• Day 4: Embryo transfer on day 4