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1. FORMULATION AND EVALUATION OF HERBAL SOAP TAKING
DIFFERENT BIOACTIVE PLANTS BY COLD SAPONIFICATION
METHOD
Presented by :- Prathmesh Ankush Shinde
Class: - Final year B. Pharmacy
Guided by :-Assistant Prof. Apeksha pangare
N.E.S’s Navsahyadri Institute of pharmacy, Naigaon, Pune.
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2. Content
1.Introduction
2. Aim and Objectives
3. Rationale of Work
4. Drug Profile
5. Literature Survey
6. Plan of Work
7. Experimental Work done
8. Future Work
9. Expected outcome
10. References
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3. Introduction
 Herbal drugs are highly effective with minimum side
effects and are to be used extensively in treating various
diseases and they are relatively low cost.
 Musa spp. Leaves, commonly known as banana leaf or
kele ka patta in India.
 It is found almost everywhere in the Indian areas, it shares
aromatic nature.
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4. Aim and objectives
Aim :- Formulation And Evaluation Of Herbal Soap Taking
Different Bioactive Plant By Cold Saponification Method .
Objectives :-
1.To promote the bright skin.
2.To reduce dark spots.
3.To help to stop dry skin .
4.To make skin smooth and soft.
5.soap an hold moisture and protect the skin
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5. Rationale of work
The rationale behind choosing the formulation of herbal
Soap is -
 Many peoples are facing the Skin problems which are
major problems , so I decided to formulate herbal Soap of
Musa Spp. Leaves (Banana leaves).
 The banana leaves are easily available.
 As banana leaves having antibacterial as well as
antioxidant property, so it moisturize the Skin , and also
helps to cure problem related to Skin.
 As the side effects of herbal Soap is less than allopathic
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6. Drug profile –Musa spp. Leaves
• Synonym: Kele ka patta , Banana leaf
• Family : Musaceae
• Biological Sources :-
Musa Spp. Leaves
• Part used: Leaves
• Taxonomy Of Plant :-
Kingdom – Plantae
Subkingdom – Tracheobionta
Superdivision - Spermatophyta
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7.  Subclass – Zingiberidae
 Genus – Musa L.
 Chemical Constituents :-
Phenolic , carotenoids, Polyphenols
 Uses:-
 Antibacterial activity
 Smoothning agent
 Hepatoprotective
 Antioxidant
 Wound healing effect.
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8. Literature survey
Sr.No. Title Authors and journals Details
1 Introduction of herbal
Soap
Manjusha kondepude, balakrishnaiah p, syamala R,
Mounik N. The formulation and evaluation of herbal bath
soap containing methanolic extract of three ayurvedic
varnya herbs October 2019 page no. 2013 -2015
Herbal bath soap uses for
skin
2 Formulation and
evaluation of herbal
Soap
Ashlesha ghanwat, sachin wayzod, vanjire divya, amit
nerkar, Formulation and evaluation of herbal soap
April 2020.page no, 21-26
Evaluation of herbal Soap
3 Introduction of Musa
spp. Herbal plant
Dexa naik, mohemmed Ali, review of introduction of
Musa spp and there advantages,june 2016.page no. 19-
24
Advantages
4 Chemical constitutes Reeta khanna, aarzoo Ali sayyed , muskan kazi , Samreen S
, introduction of chemical constituents of phenolic.
October 2022 page no . 2-65
It contain Phenolic ,
caretonoid polyphenol
5 Uses Md. Shafiqul islam, study on plant of paradise: multiple
uses of banana for sustainable development. January
2013.
1.antibacterial activity
2.hypoglycemic
3.antioxidant
4.wound healing Effect
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9. Plan of work
1)Literature survey
2)Selection of herbal plant
3)Collection of Musa Spp. Leaves
4)Drying of leaves
5) Decoction process
6)Extract preparation
7)Preliminary Phytochemical tests
8)Formulation of Soap
9)Evaluation Tests
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10. Experimental work done
(1) Collection of plant
(2) Extraction Process
(3) Preliminary phytochemical tests
(4) Formulation of herbal Soap
(5) Evaluation tests
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11. Extract
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12. Preliminary Phytochemical tests
Chemical test Leaves (observation)
1.Alkaloid Test + ve (positive test)
2.Flavonoid Test + ve (positive test)
3.Anthraquinone Test -ve (Negative test)
4.Glycoside Test + ve (positive test)
5.Tannin Test + ve (positive test)
6.steroid Test -ve ( Negative test)
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13. Formula for soap formulation
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SrNo. Ingredients Quantity
1 Coconut oil 10 ml
2 NaOH 7 ml
3 Ethanol 30 ml
4 Musa spp. Leaves 2 gm
5 Aloe vera juice 2 gm
6 Curcumin powder extract 2 gm
7 Betel leaves extract 2 gm
8 Stearic acid 2 gm
9 Reetha seed extract 1 gm
10 Soft paraffin 0.75gm
11 Lemon peel oil q.s
12 Distilled water q.s

14. Soap formulation
 Preparation of Herbal Soap:
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15. Evaluation tests
(1)Physical Evaluation
 Color
 Homogenecity
 Foaming test
(2)pH
(3)Antibacterial Activity
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16. Antibacterial Activity
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17. Result and conclusion
 Result:
 The antibacterial activity of the herbal soap was found to be very significant for S.
aureus as the highest zone of inhibition ( 26 mm) was observed with concentration
of 0.75 gm of extract of herbal
Sr No Evaluation test Result obtained
1 Physical appearance Light green
2 Homogeneity Good
3 pH 6.6
4 Foaming test Good
5 Cleansing action Good
6 Skin irritation test No irritation of skin
7 Smoothing test Good
8 Foam ability 15 cm
9 Microbial test No bacteria growth
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18. Conclusion
Based on the study results it can be concluded that herbal soap can be formulated using
cold process method, taking different Parameters in consideration as that of skin
condition and as that of herbal potentials and its activity. This sought of herbal
formulation can bring a Big difference in the field of herbal cosmetic as there are many
alignment and related flaws in different polyherbal or chemical-based formulations
Which can be removed The Physico-chemical and biological parameters of the
prepared soap Were studied. The formulation was good in appearance, with pleasant
Odor and color.
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19. Expected outcome
Finally herbal Soap of Musa spp. Leaves were formulated.
●From the antibacterial activity it was found that prepared herbal
Soap of Musa spp. leaves extract were significantly active
against gram positive bacteria.
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20. Reference
1.Manjusha kondepude, balakrishnaiah p, syamala R, Mounik N.
The formulation and evaluation of herbal bath soap containing
methanolic extract of three ayurvedic varnya herbs October 2019
page no. 2013 -2015
2.V alagupandian, selvaraj narayanan, A study on consumer
preference and satisfaction towards bath soap in madurai district,
july 2020, page no, 1-8
3.Ashlesha ghanwat, sachin wayzod, vanjire divya, amit nerkar,
Formulation and evaluation of herbal soap April 2020.page no,
21-26
4.anjalee wijetunge, gayani kanchana parera, preparation of liquid
medicinal soap products using adhatoda vasica leaf extracts,
December. 2015.page no 9-18
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21. 5.dexa naik, mohemmed Ali, review of introduction of Musa spp
and there advantages,june 2016.page no. 19-24
6.sunita padamchare , vaibhav pande, jiya jain. Review of effect
of in skin are musa spp. Leaves. September 2015. Page no. 21-34
7. Mahadeva RAO us, khamsah mohd., abdurrazak muhammad,
bashir ado ahmad, taxonomical phychemical and
pharmacologicam review of musa sapientum var. Paradisiaca,
November 2014,page no 1356-1361
8.heba sayed mostafa, Banana plant as a source of valuable
antimicrobial compounds and its current applications in the food
sector, 01 august 2021. Page no 316-328
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