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International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 6 Issue 4, May-June 2022 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD50085 | Volume – 6 | Issue – 4 | May-June 2022 Page 719
Formulation and Evaluation of Polyherbal Energy Drink
Ms. Chetana D. Patil1
, Ms. Mayuri B. Bhandare2
, Ms. Smita P. Bedis3
1
Principal, Jaywant Institute of Pharmacy Wathar, Karad, Maharashtra, India
2
Department of Chemistry GIPER, Limb, Satara, Maharashtra, India
3
Assistant Professor, Department of Pharmaceutical Chemistry Dnyanvilas
College of Pharmacy, Dudulgaon, PCMC, Pune, Maharashtra, India
ABSTRACT
Ayurvedic formulations are mainly administered by oral route, and
most of the orally administered. Ayurvedic formulations belong to
liquid form of drug. Polyherbal energy drink was prepared by using
some traditional herbs having proved nutritional potential. The
ingredients were selected as Amla, Liquorice, Ashwagandha, Tulsi,
and Mentha. The prepared polyherbal energy drink was evaluated
immediately after preparation. The analysis of prepared drink found
to contain optimum level of pH. The developed herbal energy drink
provides good taste combined with potential health benefits. The
prepared drink is potentially capable to replace the synthetic drinks
available in the market.
KEYWORDS: Polyherbal Energy Drink, Evaluation
How to cite this paper: Ms. Chetana D.
Patil | Ms. Mayuri B. Bhandare | Ms.
Smita P. Bedis "Formulation and
Evaluation of Polyherbal Energy Drink"
Published in
International Journal
of Trend in
Scientific Research
and Development
(ijtsrd), ISSN: 2456-
6470, Volume-6 |
Issue-4, June 2022,
pp.719-722, URL:
www.ijtsrd.com/papers/ijtsrd50085.pdf
Copyright © 2022 by author(s) and
International Journal of Trend in
Scientific Research and Development
Journal. This is an
Open Access article
distributed under the
terms of the Creative Commons
Attribution License (CC BY 4.0)
(http://creativecommons.org/licenses/by/4.0)
INTRODUCTION
India has used the herbal drugs long safe and
continuous uses in alternative .Medicines well as over
the counter self-medications by Ayurveda doctors. Its
definition was “any substance that may be considered
food or part of food and provides medical or health
benefits, including the prevention and treatment of
disease. The consumption of energy drinks has
increased vigorously among the youth generation,
even after knowing the fact that the energy drinks
have adverse effects. The energy drinks available in
the market contains varying amount of caffeine but it
has its own side effects. The preparation contains
poly herbs such as Amla, Liquorice, Ashwagandha,
Tulsi, and Mentha leaves have proved
pharmacological activity with no side effects. Mentha
belongs to the herb of the family Labiateae family.
The beneficial effects of Mentha in treatment of many
gastro-intestinal disorders. Moreover, the
antimicrobial, anti-inflammatory and anti-tumor
properties of Mentha. Tulsi the “queen of herbs” has
been renowned for its therapeutic potentials for anti-
asthmatic drugs. It has also been relieved to retain
good anti-stressed and analgesics activity. Liquorice
also known as sweet wood, the dried rhizomes and
roots of the plant used as carminative expectorant and
cough Remedies. Liquorice supplements can give the
adrenal gland relief. Liquorice is recommended to
treat respiratory problems. Amla is undoubtedly a
powerhouse of nutrients. The essential minerals and
Vitamins that it contains are essential not only for our
body’s health, but also for preventing some of the
common diseases. Amla is an excellent source of
vitamin C; hence it helps boost your immunity,
metabolism and prevents viral and bacterial
infections, including cold and cough. According to
Ayurveda, Amla balances all the processes in the
body and brings equilibrium all three doshas- vata,
kapha, and pitta. Ashwagandha strengthens the
immune system. It also possess anti-oxidant property.
It helps to treat terminal illnesses like cancer,
Alzheimer’s and Parkinson’s disease. It also helps to
maintain cholesterol levels and remedy for chest
IJTSRD50085
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD50085 | Volume – 6 | Issue – 4 | May-June 2022 Page 720
pains. This herbal formulation was prepared by using
different herbs which has shown pharmacological
activity with no side effects. A mixture of herbal
drugs has been used in the current research for the
development of potent energy drink. The polyherbal
energy drink is the best choice for the replacement of
soft energy drinks usage and tackles the adverse
effects.
Figure No:-1- Liqourice Figure No:-2- Amla
Figure No: - 3 -Ashwagandha
Figure No:- 4-Tulsi Figure No:-5 -Mentha
MATERIALS AND METHODS
Amla, Liquorice, Aswagandha, Tulsi, Mentha, Honey were purchased from local market.
Method of preparation of energy drink
Formula:
Sr. no Ingredients Qty. given Qty. taken Uses
1 Amla 27.8 gm 13.9 gm Immunity booster
2 Liquorice 27.8 gm 13.9 gm Carminative expectorant
3 Ashwagandha 27.8 gm 13.9 gm Anti-oxidant
4 Tulsi 9.6 gm 4.8 gm Antibacterial, stomachic
5 Mentha 7 gm 3.5 gm Flavoring agent
6 Honey Quantity sufficient Quantity sufficient Sweetening agent
7 Methyl paraben 0.8 gm 0.4gm Preservative
8 Distilled water 800 ml 400 ml Vehicle
Table no.1:-Ingredients with their prescribed quantity in the formulation of Energy Drink.
PROCEDURE: -
All the ingredients were weighed accurately and then mixed with 400ml of water, the mixture was boiled until
total volume becomes one fourth of initial volume. Then the decoction was cooled and filtered. After cooling
addition of sufficient amount of honey is mixed with drink and then required quantity of methyl paraben was
added as preservative to the mixture. Prepared drink was filled into tight container. The final herbal drink was
then subjected for evaluation.
Evaluation of Polyherbal Energy Drink:
Physical appearance
The polyherbal energy drink was examined for physical appearance in terms of colour, Odour.
Test for carbohydrates
1-2 drops of dilute iodine + 1-2 ml of sample which shows red or brown colour.
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD50085 | Volume – 6 | Issue – 4 | May-June 2022 Page 721
Test for alkaloids
Dragondroff’s test : 2-3 ml sample +few drops of
Dragondroff’s reagent solution turns into orange
brown ppt.
Test for flavonoides
Small amount of sample mixed with lead acetate
solution which shows yellow ppt.
Test for tannins and phenolic compounds
Small amount of sample + acetic acid which gives red
colour.
Test for proteins
2-3 ml of sample + 2 ml of biuret reagent then mix
vigorously solution shows purple colour.
Test for fats :
Take small amount of sample on piece of filter paper,
dry the paper in sunlight, observethepaper, oilypatch
on the paper indicates that presence of fat.
Determination of pH
The pH of energy drink was checked by using a
calibrated digital pH meter at constant temperature
and the pH was noted.
RESULT AND DISCUSSION:
.
Figure No: - 6-Polyherbal Energy Drink
Sr. no Parameter Observation
1 Colour Reddish brown
2 Odour Pleasant
3 Taste Sweet
4 pH 4.35
Table no.2: -Evaluation Table of Polyherbal
Energy Drink
Figure No: - 6- Phytochemical parameter
Sr.
no
Phytochemicals Observations
1 Carbohydrates Present
2 Alkaloids Present
3 Flavonoids Present
4 Proteins Absent
5
Tannins and phenolic
compounds
Present
6 Fats Absent
Table no.3: -- Phytochemical parameter of
Polyherbal Energy Drink
DISCUSSION:
The prepared polyherbal energy drink was evaluated
immediately after preparation and all the parameters
are tested. The final polyherbal energy drink found to
have a pH 4.35. The observed parameters like color,
odour, and taste have been tested. In order to evaluate
the suitability of the formulation for nutritional
purpose physicochemical and phytochemical
parameters were carried out. Preliminary
phytochemical analysis of polyherbal energy drink
showed the presence of alkaloids, carbohydrates,
tannins, flavonoids, and phenolics.Phenolics and
flavonoids are wide class of chemical compounds
found in plants. They report quality and nutritional
value and plays a vital role in human fitness. Crude
tannin is the compounds present in the plants.
Tannins are the polyphenols that are responsible for
the astringent flavor of food and shows anti-
carcinogenic.
CONCLUSION:
The prepared formulation beneficial for the all the
persons. The formulation is prepared from the natural
herbs so the chances of side effects are lower than the
soft drinks. This herbal energy drink is natural option
to the synthetic drinks along with the several health
benefits. This is good supplement for freshly recover
from the illness and gives the freshness to the person.
All the herbs used in this preparation are easily
available during any season and are not costly thus
the product is economically feasible.
REFERENCES:
[1] GavaliVikas B, Bhalsing Mahesh, DalviNilam
B, TarkasbandYogita S. Development and
evaluation of polyherbalpowder formulation as
energy booster; journal of
pharmacognosyandphytochemistry 2018.
[2] MunishGarg, Vinni Ahuja, Development and
evaluation of a Nutraceutical herbal summer
drink; international journal of pharmacological
and pharmaceutical sciences vol 9, no 7 2015.
[3] Swain Pramod Kumar, NayakDurgaPrasan,
Design, Devlopment and evaluation of a
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD50085 | Volume – 6 | Issue – 4 | May-June 2022 Page 722
Polyherbalsyrup from the some herbs used as
energy booster; International journal of
Ayurvedic medicine, 2013, 4(4) 374-378.
[4] Abhinav Pandey, Rishabh Dixit, Alok Prakash
and V. Devi Rajeshwari, school of bioscience
and Technology , VIT university, vellore,
Tamil Nadu, India, Development of an herbal
energy drink using medicinal plant extracts and
further statistically analyzing the sensory
properties by analyzing of variance.(ANOVA).
IJPSR(2014), vol 5 Issue 2.
[5] Yunusa, I. and Ahmad I. M. energy drinks :
consumption and health benefits. Bayero
journal of pure and applied sciences,4(2):186-
191.
[6] Michal J. Gonzalez, Jorge R. Miranda-Massari,
Jose Rodriguez Gomez, Carlos M. Ricart,
Dharma Rodriguez-Pagan. Energy drinks and
health: A brief review of their effects and
Consequences.
[7] Natalie Ohanessian , developing a green tea
based natural energy drink. 2011.
[8] N. Dev, A. K. Das , M. A. Hossain, and S. M.
M. Rahman, Chemical composition of different
[9] Extracts of Ocimumbasilium leaves, journal of
scientific research 3(1), 197-206, 2011.
RaneRajshree, GangolliDivya, PatilSushma,
and IngavaleKanchan, Amla, Ashwagandha
and Shatavari formulations as herbal medicines
and nutraceuticals. research journal of
pharmaceutical sciences vol. 1(3), 10-15.
November 2012.
[10] Poonam Mishra and CharuLataMahanta,
Extraction, identification and anti-oxidant
properties of bioactive component of
amlapomace powder. international journal of
food and nutritional science vol.5, no.3, july
2016.
[11] M. A. Heckman, K. Sherry, And E. Gonzalez
de Mejja. Energy drinks: An assessment of
their market size, consumer demographics,
ingredient profile, functionality and regulations
in the united states.
[12] Nauman Aziz, Malik Hassan Mehmood and
Anwarul Hassan Gilani, Studies on two
polyhervbal formulations for comparison of
their anti-dyslipidemic, anti-hypertensive and
endothelial modulating activities.
Complementary and alternative medicines
2013.
[13] Khatwani P. K. ,Gurale V.V, Kulkarni S. R.
Evaluation of polyherbaloral formulation for
anti-diabetic activity. International journal of
pharmacology.6(4),2015. 184-190.
[14] LCDR Lucas A. Johnson, MC USN; LCDR
David Foster, MC USN; LCDR Jackie C.
McDowell, MC USN, Energy drinks: review of
performance benefits, health concerns and use
by military personnel.
[15] NameerNassirFaris, Energy drinks: Factors that
influence college students consumption 2014.

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Formulation and Evaluation of Polyherbal Energy Drink

  • 1. International Journal of Trend in Scientific Research and Development (IJTSRD) Volume 6 Issue 4, May-June 2022 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470 @ IJTSRD | Unique Paper ID – IJTSRD50085 | Volume – 6 | Issue – 4 | May-June 2022 Page 719 Formulation and Evaluation of Polyherbal Energy Drink Ms. Chetana D. Patil1 , Ms. Mayuri B. Bhandare2 , Ms. Smita P. Bedis3 1 Principal, Jaywant Institute of Pharmacy Wathar, Karad, Maharashtra, India 2 Department of Chemistry GIPER, Limb, Satara, Maharashtra, India 3 Assistant Professor, Department of Pharmaceutical Chemistry Dnyanvilas College of Pharmacy, Dudulgaon, PCMC, Pune, Maharashtra, India ABSTRACT Ayurvedic formulations are mainly administered by oral route, and most of the orally administered. Ayurvedic formulations belong to liquid form of drug. Polyherbal energy drink was prepared by using some traditional herbs having proved nutritional potential. The ingredients were selected as Amla, Liquorice, Ashwagandha, Tulsi, and Mentha. The prepared polyherbal energy drink was evaluated immediately after preparation. The analysis of prepared drink found to contain optimum level of pH. The developed herbal energy drink provides good taste combined with potential health benefits. The prepared drink is potentially capable to replace the synthetic drinks available in the market. KEYWORDS: Polyherbal Energy Drink, Evaluation How to cite this paper: Ms. Chetana D. Patil | Ms. Mayuri B. Bhandare | Ms. Smita P. Bedis "Formulation and Evaluation of Polyherbal Energy Drink" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456- 6470, Volume-6 | Issue-4, June 2022, pp.719-722, URL: www.ijtsrd.com/papers/ijtsrd50085.pdf Copyright © 2022 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0) INTRODUCTION India has used the herbal drugs long safe and continuous uses in alternative .Medicines well as over the counter self-medications by Ayurveda doctors. Its definition was “any substance that may be considered food or part of food and provides medical or health benefits, including the prevention and treatment of disease. The consumption of energy drinks has increased vigorously among the youth generation, even after knowing the fact that the energy drinks have adverse effects. The energy drinks available in the market contains varying amount of caffeine but it has its own side effects. The preparation contains poly herbs such as Amla, Liquorice, Ashwagandha, Tulsi, and Mentha leaves have proved pharmacological activity with no side effects. Mentha belongs to the herb of the family Labiateae family. The beneficial effects of Mentha in treatment of many gastro-intestinal disorders. Moreover, the antimicrobial, anti-inflammatory and anti-tumor properties of Mentha. Tulsi the “queen of herbs” has been renowned for its therapeutic potentials for anti- asthmatic drugs. It has also been relieved to retain good anti-stressed and analgesics activity. Liquorice also known as sweet wood, the dried rhizomes and roots of the plant used as carminative expectorant and cough Remedies. Liquorice supplements can give the adrenal gland relief. Liquorice is recommended to treat respiratory problems. Amla is undoubtedly a powerhouse of nutrients. The essential minerals and Vitamins that it contains are essential not only for our body’s health, but also for preventing some of the common diseases. Amla is an excellent source of vitamin C; hence it helps boost your immunity, metabolism and prevents viral and bacterial infections, including cold and cough. According to Ayurveda, Amla balances all the processes in the body and brings equilibrium all three doshas- vata, kapha, and pitta. Ashwagandha strengthens the immune system. It also possess anti-oxidant property. It helps to treat terminal illnesses like cancer, Alzheimer’s and Parkinson’s disease. It also helps to maintain cholesterol levels and remedy for chest IJTSRD50085
  • 2. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD50085 | Volume – 6 | Issue – 4 | May-June 2022 Page 720 pains. This herbal formulation was prepared by using different herbs which has shown pharmacological activity with no side effects. A mixture of herbal drugs has been used in the current research for the development of potent energy drink. The polyherbal energy drink is the best choice for the replacement of soft energy drinks usage and tackles the adverse effects. Figure No:-1- Liqourice Figure No:-2- Amla Figure No: - 3 -Ashwagandha Figure No:- 4-Tulsi Figure No:-5 -Mentha MATERIALS AND METHODS Amla, Liquorice, Aswagandha, Tulsi, Mentha, Honey were purchased from local market. Method of preparation of energy drink Formula: Sr. no Ingredients Qty. given Qty. taken Uses 1 Amla 27.8 gm 13.9 gm Immunity booster 2 Liquorice 27.8 gm 13.9 gm Carminative expectorant 3 Ashwagandha 27.8 gm 13.9 gm Anti-oxidant 4 Tulsi 9.6 gm 4.8 gm Antibacterial, stomachic 5 Mentha 7 gm 3.5 gm Flavoring agent 6 Honey Quantity sufficient Quantity sufficient Sweetening agent 7 Methyl paraben 0.8 gm 0.4gm Preservative 8 Distilled water 800 ml 400 ml Vehicle Table no.1:-Ingredients with their prescribed quantity in the formulation of Energy Drink. PROCEDURE: - All the ingredients were weighed accurately and then mixed with 400ml of water, the mixture was boiled until total volume becomes one fourth of initial volume. Then the decoction was cooled and filtered. After cooling addition of sufficient amount of honey is mixed with drink and then required quantity of methyl paraben was added as preservative to the mixture. Prepared drink was filled into tight container. The final herbal drink was then subjected for evaluation. Evaluation of Polyherbal Energy Drink: Physical appearance The polyherbal energy drink was examined for physical appearance in terms of colour, Odour. Test for carbohydrates 1-2 drops of dilute iodine + 1-2 ml of sample which shows red or brown colour.
  • 3. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD50085 | Volume – 6 | Issue – 4 | May-June 2022 Page 721 Test for alkaloids Dragondroff’s test : 2-3 ml sample +few drops of Dragondroff’s reagent solution turns into orange brown ppt. Test for flavonoides Small amount of sample mixed with lead acetate solution which shows yellow ppt. Test for tannins and phenolic compounds Small amount of sample + acetic acid which gives red colour. Test for proteins 2-3 ml of sample + 2 ml of biuret reagent then mix vigorously solution shows purple colour. Test for fats : Take small amount of sample on piece of filter paper, dry the paper in sunlight, observethepaper, oilypatch on the paper indicates that presence of fat. Determination of pH The pH of energy drink was checked by using a calibrated digital pH meter at constant temperature and the pH was noted. RESULT AND DISCUSSION: . Figure No: - 6-Polyherbal Energy Drink Sr. no Parameter Observation 1 Colour Reddish brown 2 Odour Pleasant 3 Taste Sweet 4 pH 4.35 Table no.2: -Evaluation Table of Polyherbal Energy Drink Figure No: - 6- Phytochemical parameter Sr. no Phytochemicals Observations 1 Carbohydrates Present 2 Alkaloids Present 3 Flavonoids Present 4 Proteins Absent 5 Tannins and phenolic compounds Present 6 Fats Absent Table no.3: -- Phytochemical parameter of Polyherbal Energy Drink DISCUSSION: The prepared polyherbal energy drink was evaluated immediately after preparation and all the parameters are tested. The final polyherbal energy drink found to have a pH 4.35. The observed parameters like color, odour, and taste have been tested. In order to evaluate the suitability of the formulation for nutritional purpose physicochemical and phytochemical parameters were carried out. Preliminary phytochemical analysis of polyherbal energy drink showed the presence of alkaloids, carbohydrates, tannins, flavonoids, and phenolics.Phenolics and flavonoids are wide class of chemical compounds found in plants. They report quality and nutritional value and plays a vital role in human fitness. Crude tannin is the compounds present in the plants. Tannins are the polyphenols that are responsible for the astringent flavor of food and shows anti- carcinogenic. CONCLUSION: The prepared formulation beneficial for the all the persons. The formulation is prepared from the natural herbs so the chances of side effects are lower than the soft drinks. This herbal energy drink is natural option to the synthetic drinks along with the several health benefits. This is good supplement for freshly recover from the illness and gives the freshness to the person. All the herbs used in this preparation are easily available during any season and are not costly thus the product is economically feasible. REFERENCES: [1] GavaliVikas B, Bhalsing Mahesh, DalviNilam B, TarkasbandYogita S. Development and evaluation of polyherbalpowder formulation as energy booster; journal of pharmacognosyandphytochemistry 2018. [2] MunishGarg, Vinni Ahuja, Development and evaluation of a Nutraceutical herbal summer drink; international journal of pharmacological and pharmaceutical sciences vol 9, no 7 2015. [3] Swain Pramod Kumar, NayakDurgaPrasan, Design, Devlopment and evaluation of a
  • 4. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD50085 | Volume – 6 | Issue – 4 | May-June 2022 Page 722 Polyherbalsyrup from the some herbs used as energy booster; International journal of Ayurvedic medicine, 2013, 4(4) 374-378. [4] Abhinav Pandey, Rishabh Dixit, Alok Prakash and V. Devi Rajeshwari, school of bioscience and Technology , VIT university, vellore, Tamil Nadu, India, Development of an herbal energy drink using medicinal plant extracts and further statistically analyzing the sensory properties by analyzing of variance.(ANOVA). IJPSR(2014), vol 5 Issue 2. [5] Yunusa, I. and Ahmad I. M. energy drinks : consumption and health benefits. Bayero journal of pure and applied sciences,4(2):186- 191. [6] Michal J. Gonzalez, Jorge R. Miranda-Massari, Jose Rodriguez Gomez, Carlos M. Ricart, Dharma Rodriguez-Pagan. Energy drinks and health: A brief review of their effects and Consequences. [7] Natalie Ohanessian , developing a green tea based natural energy drink. 2011. [8] N. Dev, A. K. Das , M. A. Hossain, and S. M. M. Rahman, Chemical composition of different [9] Extracts of Ocimumbasilium leaves, journal of scientific research 3(1), 197-206, 2011. RaneRajshree, GangolliDivya, PatilSushma, and IngavaleKanchan, Amla, Ashwagandha and Shatavari formulations as herbal medicines and nutraceuticals. research journal of pharmaceutical sciences vol. 1(3), 10-15. November 2012. [10] Poonam Mishra and CharuLataMahanta, Extraction, identification and anti-oxidant properties of bioactive component of amlapomace powder. international journal of food and nutritional science vol.5, no.3, july 2016. [11] M. A. Heckman, K. Sherry, And E. Gonzalez de Mejja. Energy drinks: An assessment of their market size, consumer demographics, ingredient profile, functionality and regulations in the united states. [12] Nauman Aziz, Malik Hassan Mehmood and Anwarul Hassan Gilani, Studies on two polyhervbal formulations for comparison of their anti-dyslipidemic, anti-hypertensive and endothelial modulating activities. Complementary and alternative medicines 2013. [13] Khatwani P. K. ,Gurale V.V, Kulkarni S. R. Evaluation of polyherbaloral formulation for anti-diabetic activity. International journal of pharmacology.6(4),2015. 184-190. [14] LCDR Lucas A. Johnson, MC USN; LCDR David Foster, MC USN; LCDR Jackie C. McDowell, MC USN, Energy drinks: review of performance benefits, health concerns and use by military personnel. [15] NameerNassirFaris, Energy drinks: Factors that influence college students consumption 2014.