This ppt is about the canaball tree i.e Kailash pati plant .which is very rearliy avilable. It's a medicinal plant and have a so many pharmaceutical or medicinal value. The ppt is on its wound healing properties

1. BOTANICAL AUTHENTICATION OF
COUROUPITA GUIANENSIS AUBL
TREE AND IT’S FORMULATION
AND EVALUTION OF LEPA
Name of Student :-Darshan Madhukar Pawar
Roll No : -51
P.R.N No : - 1131920038282
Under the Guidance : - prof. Dr. V. D Wagh

2. Content
INTRODUCTION TO COUROUPITA GUIANENSIS PLANT
AIM AND OBJECTIVE
PHARMACOLOGICAL ACTIVITIES
TRADITIONAL USES
REVIEW OF LITRATURE
PLAN OF WORK
INTODUCTION TO LEPA
MATERIAL AND METHOD OF PREPARATION
EVALUTION PARAMETRES
RESULT
REFERENCES

3. Introduction
 Kailaspati botanical name Couroupita guianensis Aubl.
It is grown in Indian gardens as an ornamental tree for its beautiful
flowers.
It is known as ‘Cannonball’ tree in English and ‘Kailaspati’ in Hindi
it belongs to the family Lecythidaceace.
Couroupita guianensis is a large deciduous evergreen tree growing to
a height of 20 meters.
It is a medicinal plant which is endowed with curative properties
including antifungal, antibiotic, antiseptic, analgesic, antimalarial,
stomachache, toothache, scabies, gastritis, bleeding piles, dysentery,
and scorpion poison.
 The fresh fruit pulp is used in the preparation of cooling medicinal
drink, and various parts are useful in skin disease.

4. Fig.1- Flower Fig.2-fruit & flower fig.3-leaves
fig.3- Fruit fig.4-seeds fig.4wholetree

5. AIM-
To prepare wound healing Lepa from the extract of
whole plant of couroupita guianensis aubl
OBJECTIVES-
• To prepare wound healing Lepa
•To expose couropita guianensis aubl as natural source for
therapeutic uses

6. PHARMACOLOGICAL ACTIVITIES
1.ANTIBACTERIAL ACTIVITY
Antibacterial activity of Ethyl alcohol extract of fruit pulp was evaluated on gram-positive and
gram-negative bacteria and it showed antibacterial activity. Phytoconstituents like tannins and
poluphenol are responsible for activity
2. ANTIOXIDENT ACTIVITY
Ethyl acetate fraction of water extract showed robust antioxident property in vitro using DPPH
assay method
3.ANTIDEPRESSANT ACTIVITY
Methanolic extract showed significant antidepressant activity in tail suspension check. Result
showed considerable decrease in immobility in tail suspension test .

7. 4.WOUND HEALING ACTIVITY
Ethanolic extract of whole plant(bark, leaves, flowers and fruit) showed wound healing
properties . This extract hurries wound healing .
5.ANTIDIARRHEAL ACTIVITY
In castor oil evoked diarrhea methanolic and water extract vital reduction in diarrhea
episode at 100mg/kg dose.
6.ANTIARTHRITIC ACTIVITY
Leaves of methanolic extract showed considerable activity by victimization invitro
technique

8. TRADITIONAL USE OF COUROUPITA GUIANENSIS
The pulp of the fruit of the cannon ball tree is rubbed on the infected
skin of animal disease dog.
The within of the fruit will make clean wounds and young leaves cure
odontalgia.
Traditionally leaves as used as antiseptic and odontalgia.
Juice made up of the leaves is employed to cure skin ailments.
Historically, the leaves of this plant are utilized in the treatment of skin
diseases, stomach, ache, and enteral gas formation, antithrombotic and
vasodilatory actions
Leaves and flowers of Couroupita guianesis unit used for healthful
applications like upset, tumors, pain and inflammatory processes, cold,
enteric gas formation and abdomen ache.

9. Flowers Carbohydrates, Protein,
Alkaloids, Terpenoid
Leaves Eugenol, farnesol
Fruit stigmasterol and
campesterol
seeds Linoleic acid, nerol,
tryptanthrin
PHYTOCHEMICALS

10. Review of literature
SR.NO. NAME OF AUTHOR OBSERVATION LITERATURE USED FOR
1 Shah ,et al., (2012) Antibacterial activity of
Couroupita guianensis fruit pulp
extract
Antibaterial activity
2 Bafna, et al., (2011) In vitro antioxidant activity of
ethyl acetate fraction of water
extract of flowers of Couroupita
guaianensis
Antioxident activity
3 Vinod. , et al, (2013) Neuropharmacological evaluation
of the methanolic extract of
Couroupita guianensis flower in
mice
Anxiolytic activity
4 Elumalai, et al.,(2012) Evaluation of antiulcer activity of
Couroupita guianensis leaves
Antialcer activity

11. 4 Wankhede, et al.,
(2009)
Couroupita guianensis. Evaluation of its
antidepressant activity in mice
Antidepressant Activity
5 Geetha, et al.,
(2005)
Antifertility activity of Artabotrys
odoratissimus Roxb. and Couroupita
guianensis
Antifertility activity
6 Al-Dhabi, et al.,
(2012)
Antimicrobial, antimycobacterial and
antibiofilm properties of Couroupita
guianensis fruit extract
Antimicrobial activity
7 Usman, et al.,
(2012)
Evaluation of antipyretic activity of
Anthocephalus cadamba roxb. leaves
extracts
Antipyretic activity

12. 8 Umachigi, et al.,
(2007)
Antimicrobial, wound healing and
antioxidant potential of Couroupita
guianensis in rats.
Woiund healing property
9 Elumalai ,et al.,
(2012)
Antioxidant, antiarthritic and
antiplatelet studies in Couroupita
guianensis aubl leaves by invitro
methods.
Antiarthritic Activity
10 Lingathurai, et al.,
(2011)
Antifeedant and larvicidal activities
of leaves were studied.
Antifeedent and Larvcidal
Activity
11 Pinheiro , et al.,
(2010)
Antinociceptive activity of from
Couroupita guianensis Aubl. Leaves
Antinociceptive Activity

13. 12 Bhaskar, et al., (2012) Ethyl acetate extract of
Couroupita guianensis
leaves tested for larvicidal
activity
Larvicidal activity
13 Bhaskar, et al., (2015) Chloroform extract is
studied for ovicidal activity
Ovicidal activity
14 Row , et al., (1966) α-amirin, β-amirin, β-
sitosterol, tannins.
Phytochemicals
15 Ramalaxmi, et al.,
(2013)
Alkaloid carbohydrate
protein
Phytochemicals

14. 16 K. Ashok Kumar, et.al
,October (2013)
From this artical we have
taken introduction part
Introduction of plant
17 Vijay Shankar Verma
et.al
From this artical we have
taken intoduction to lepa
Introduction to lepa
18 Tejaswini Singh et.al From this article we have
taken reference for method
of preparation lepa. .
Method of preparation
19 Tejaswini Singh et.al From this we have a taken
reference for evalution
parameters
Evalution parameters

15. 20 Dr. Rajendra G. Wagh
et.al
Fom this article we have
taken the reference for
conculsion
Conclusion

16. INTRODUCTION TO LEPA
Defination:
Medicines in the form of paste used for external application are called lepas.
Lepa is the herbal preparation used for external use. The main basic concept
of Lepa is that, wet drugs are pounded to fine paste form and the dry drugs
are pounded to fine powder form and mixed with any of the liquid media like
Gomutra,ghee, Jala etc. according to demands of particular disease condition
or need. This paste is used for application as Lepa.
Types of lepa :
I. Pralepa (prepared from plant having cold potency)
II. Pradeha ( prepared from plant having hot poteny)
III. Alepa ( mixture of pralepa and pradeha )
Application of lepa :
I. Lepa used in different disease condition.
II. Lepa are used for wound healing, wound cleaning

17. PLAN OF WORK
Collection of plant
raw material and its
authentication
Ethanolic extract of
whole plant of (barks
,leaves, flowers, fruits )
couroupita guianensis
Formulation of wound
healing Lepa
Evaluation of prepared
Lepa

18. MATERIAL AND METHOD
Plant collection and identification
The sample of c. guianensis were collected from the nashik road, nashik near to bitco
collage. The c. guianesis plant was identified and then colleted.The plant sample such as
leaves, stem, bark, fruits, and flowers were collected washed with distilled water. From the
flowers , petals and from fruit the fruit plup seperated for drying and analysis. Afterward
,the samples were dried on blotting shit for 4 to 5 days. Dried sample were taken out and
homogenized into a core powder using mortar and pestle, stored in air tight container and
were used for extraction process.

19. MATERIAL AND METHOD
 Preparation of powdering of ingredients:
In main pharmaceutical process of powdering firstly, we have taken dired form of raw
material .Then the each part of plant[leaves ,fruit ,flower ,steam,bark,] is pulverized
separately to make it powder form. Then each powder ingredient of CG lepa is sieved
with the help of mesh no 40. to make it fine. The sieved powder was measured and
collected in air tight container for further procedure.
Preparation of CG lepa :
Equal quantity of each powder ingredient of CG lepa was mixed with the help of spatula in
a stainless steel vessel to prepare a homogenous mixture. This mixture was taken in mortar
and triturated with cows urine [cows urine as a medium]. Cows urine was added in
sufficient quantity so that it facilitates proper movement of semi solid mass. This paste is
then applied on the wound of the patient to cure wound. keep it for 1to 2 hrs.

20. EVALUTION PARAMETERS
Organoleptic evalution
Organoleptic features like color, odor ,taste ,and consistency of the lepa were recorded and
given below.
i. Organoleptic characters of c.guianensis lepa
1 .Drug : Couroupita guianensis lepa
2. Color : Brown
3. Odor :Characteristic
4. Taste : Slight
5 . Consistency : Fine powder

21. EVALUTION PARAMETERS
Skin irritation test
Skin irritation test was performed to determine skin sensitivity by applying Lepa and its
routine structures to intact skin. Following application, the Lepa was kept in contact with
intact skin for 15 minutes and then washed off with normal water. The process lasted for
one week. Redness, rashes, burning sensations, itching, and other unfavorable reactions
were observed on the skin. In addition, the Lepa formulation left the skin smooth with no
signs of skin dryness normally associated with Lepas.
Homogenisity: Homogenisity of prepared formulation is tested by visual inspection.
pH : ph is determined by litmus paper and changed colour of litmus paper.
VIiscosity: Viscosity is determined using Ostwald viscometer
Colour and appearane : Colour and appearance is recorded by visual inspection

22. Result
Result organoleptic character :
SR . NO PARAMETERS F1 F2 F3
1 Nature Core powder Powder Fine powder
2 PH 5.10 5.15 5.12
3 Particle size 20-25 19-23 18-25
4 Color and appearance Brown Little brown Light brown
5 spreadability Not easily spread No spreadability Easily
spreadability
6 Skin irritation Normal irritation No irritation No irritation
7 Consistency Good Low Good
8 Bulk density 0.30 0.29 0.28

23. CONCLUSION
Lepa are the key medicine for external application on skin. Lepa are used in different
disease condition like wound cleaning, wound healing, faireness of skin, inflammation, acne
and many more disease condition.In this way we can say that Lepa plays an important role
in Ayurved system of medicine. This formulation is prepared for wound healing purpouse .
Various taste implied that the formulation passed various evalution parameters when it is
safe to use. Various Batches was prepared for this. On each batch evolution parameters was
studied and performed.

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28. THANK YOU..