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Pleasant Smell




2
Unpleasant Smell




3
Can insects be repelled by bad odour ?-
      Mechanism of olfaction in insects


                  Pramod Kumar K.
                     2010-11-150
           Dept. of Agricultural Entomology


4
Contents
     Definition
     Distribution of olfactory receptors
     Structure of olfactory receptors
     Cuticular components
     Cellular components
     Mechanism of olfaction
     Semiochemicals
     Insect attractants
     Insect Repellants
     Examples for insects repellence
     Conclusion

5
Olfaction
     The sense of smell




6
Distribution of Olfactory Receptors




7
 Olfactory sensilla are present on




                           Antennae
                        (Praying mantis)


    Labial palps                             Maxillary palps
8    (Butterfly)                              (Mosquitoes)
Structure of Olfactory Receptors

     Cuticular components    Cellular components




9
Cuticular Components

      Multiporous



      Pores accommodate

      olfactory receptors
      helps in perception of
      smell


10
Different Types of Sensillum

      Trichoid sensilla


      Basiconic sensilla


      Coeloconic sensilla


      Placoid sensilla




11
                              (Chapman, 2006)
Trichoid Sensilla
      Hair like structures (thin walled or thick walled)


      eg. Antennae of Bombyx mori




12
Basiconic Sensilla
      Peg or cone like organs

      eg. Maxillary palps of female culicidae




13
Coeloconic Sensilla
      Cuticular peg is sunk below the general surface of
      the cuticle.

                                           Opening at surface of
                                           cuticle
                                           Cavity
                                           Peg
                                           Dendrite
                                           Cuticle

                                           Dendrite sheath




14
Placodea Sensilla

      Flat or raised in to a slight dome


       eg. Antennae of honey bee

                                            Pore
                                            Dendrite


                                            Neuron




15
Cellular Components
      Cells of olfactory sensilla are derived from the
      epidermis
      Trichogen cell




16
Cellular Components               contd…

      Number of sensory neurons in olfactory sensilla
      varies from 2 to 20




         Male moths                   Grasshopper

17
Mechanism of Olfactory Reception




18
Insect Olfactory Sense

      Odorants (smells/volatile chemicals) have to travel

      through pores in the cuticle



      Distinctive smell from environments like

      semiochemicals




19
Semiochemicals

     Pheromones                Allelochemicals
      Sex pheromone            Allomones

      Aggregation pheromone    Kairomones

      Alarm pheromone          Synamones

      Trail pheromone          Apneumones




                               (Ragumoorthui et al., 2007)
20
Pheromone
      Intraspecific chemical substances that mediate

      communication between insects

      Ectohormones

      1959: Karlson and Butenandt

      Silkworm moths




21
Sex Pheromone
      Pheromone released by one sex only and trigger
       behaviour patterns in the other sex that facilitate in
       mating
      Female insect produce pheromone
       eg. Pink bollworm – gossyplure
           Helicoverpa armigera - helilure




22
Female Sex Pheromone




      Bactrocera dorsalis   Methyl eugenol
23
Female Sex Pheromone        contd…




      Bactrocera cucurbitae   Cuelure


24
Aggregation Pheromone
      Pheromones which induce aggregation or
       congregation of insects for protection,
       reproduction and feeding
      Coleoptera and Dictyoptera




      Cockroach – periplanone       Phloem beetle - ipsenol
25
Aggregation Pheromone               contd…
      Red palm weevil – Rhynchophorus ferrugenius
      Ferrolure




26
Alarm Pheromone
      Chemical substance released by insects to warn
      members of same species about the presence or
      attack by an enemy

      eg. Aphids




27
Trail Pheromone
      Pheromones laid in form of intermittent or
      continuous lines on a soil substrate which trail
      followers perceive to reach their destination which
      could be a mate or food source

       eg. Ants




28
Allelochemicals

      Interspecific chemicals mediate the communication
      between two different species of insects



      They are classified in to four categories




29
Allomones
      Chemical released by one insect that induce a
       response in another insect
      Helpful to emitter


      eg. Sting glands in bees




30
Kairomones
      Chemical released by one insect that induce a
       response in another insect
      Helpful to recipient


       eg. Heptanoic acid releases by potato tuber moth




31
Synamone
      Chemical released by one insect that induce a
       response in another insect
      Helpful to emitter and receiver


      eg. Termites and protozoan's




32
Apneumones
      A chemical substance emitted by non living material
      that evoke the behavioural or physiological reaction
      adaptively for receiving organism

      eg. Oat meal oil




33
Insect Attractants

      Chemicals that cause insect to make oriented
      movements towards their source

      Types of attractants are


        Pheromone


        Natural food lures



34
Natural Food Lures
      Chemicals present in plant and animal hosts that
      attract insect for feeding



      Floral scents nectar feeding insect




      Decomposing products



35
Fish Meal Trap
      Sorghum shoot fly is attracted to rotten fish meal




36
Coconut shell trap




37
Insect Repellants

      An oriented movement of insects away from

      their source of food




38
Insect Olfactory Sense contd...

      Smell travels through air
      Then they diffuse and are
       carried by an odorant
       binding protein (OBP)
      Many odorants are received
       by insect olfactory
       sensillum and perceived by
       the olfactory receptor
       neurons (ORNs)

               (Galizia and Rossler, 2010)
39
Neuron




40
Sensilla
                                Antennae

     Na+ , K+ channel
                            Glomeruli




41
Protocerebrum
     Projection neuron




                 Response




42                          Motor neuron
Insect Olfactory Sense contd...




     OBP




43
Insect Olfactory Sense             contd...
      Receptor neurons project an axon through the
      olfactory nerve into the primary olfactory centers –
      olfactory bulb




44
Insect Olfactory Sense   contd...

       Deutocerebrum

      Have the olfactory lobe


      Receives signal from
       glomeruli

      Projection neurons will
       send the signal to
       protocerebrum
45
Insect Olfactory Sense             contd...

      Projection neurons, connecting olfactory lobes to
      mushroom bodies and other parts of protocerebrum.




46
Insect Olfactory Sense             contd...

      The physiological characteristics of the projection
      neurons vary

      They respond only when the antennal receptors are
      simulated by a specific odour like a pheromone,
      attractant or repellent components

      Others are simply ignored or not recognized



47
Olfactometer
      Specific reaction to olfactory stimuli to be verified
       by olfactometer responses




48
Examples for insect repellence by
               bad odour




49
Repelling mosquitoes by burning neem leaves
     along with cow dung




50                            (Mandavgane et al., 2005)
To repel honey robber, some stingless social bees
     erect walls of sticky resin in front of or around the
     nest entrance, while other species smear a
     repugnant liquid there




51
Eucalyptus oil – pleasant smell in human, repellent
     effect on insects like mosquitoes and fleas




52                                   (Lett and Kraus, 1994)
Cocoa leaves, eucalyptus oil, garlic extract, olive
     oil mixture of these ingredients will repell the ants,
     cockroach, mosquitoes and spiders




53
Osmeterium, an orange, Y-shaped structure on the
     neck of caterpillar gives off a strong, unpleasant
     odour when it is threatened




54
55
Conclusion
  Odour perception is like visiting a library. Thousands of
     books are present in the library. Similarly there are a
     number of odorants present in the environment. All
     groups of insects have specific odours which they
     receive through specific glomeruli.




56
 Only odour stimuli in the case of insects. They
      receive only those known to them

      There is nothing like good or bad odour




57
58

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Pramod seminar checked

  • 1. 1
  • 4. Can insects be repelled by bad odour ?- Mechanism of olfaction in insects Pramod Kumar K. 2010-11-150 Dept. of Agricultural Entomology 4
  • 5. Contents  Definition  Distribution of olfactory receptors  Structure of olfactory receptors  Cuticular components  Cellular components  Mechanism of olfaction  Semiochemicals  Insect attractants  Insect Repellants  Examples for insects repellence  Conclusion 5
  • 6. Olfaction  The sense of smell 6
  • 8.  Olfactory sensilla are present on Antennae (Praying mantis) Labial palps Maxillary palps 8 (Butterfly) (Mosquitoes)
  • 9. Structure of Olfactory Receptors  Cuticular components  Cellular components 9
  • 10. Cuticular Components  Multiporous  Pores accommodate olfactory receptors helps in perception of smell 10
  • 11. Different Types of Sensillum  Trichoid sensilla  Basiconic sensilla  Coeloconic sensilla  Placoid sensilla 11 (Chapman, 2006)
  • 12. Trichoid Sensilla  Hair like structures (thin walled or thick walled) eg. Antennae of Bombyx mori 12
  • 13. Basiconic Sensilla  Peg or cone like organs eg. Maxillary palps of female culicidae 13
  • 14. Coeloconic Sensilla  Cuticular peg is sunk below the general surface of the cuticle. Opening at surface of cuticle Cavity Peg Dendrite Cuticle Dendrite sheath 14
  • 15. Placodea Sensilla  Flat or raised in to a slight dome eg. Antennae of honey bee Pore Dendrite Neuron 15
  • 16. Cellular Components  Cells of olfactory sensilla are derived from the epidermis  Trichogen cell 16
  • 17. Cellular Components contd…  Number of sensory neurons in olfactory sensilla varies from 2 to 20 Male moths Grasshopper 17
  • 18. Mechanism of Olfactory Reception 18
  • 19. Insect Olfactory Sense  Odorants (smells/volatile chemicals) have to travel through pores in the cuticle  Distinctive smell from environments like semiochemicals 19
  • 20. Semiochemicals Pheromones Allelochemicals  Sex pheromone  Allomones  Aggregation pheromone  Kairomones  Alarm pheromone  Synamones  Trail pheromone  Apneumones (Ragumoorthui et al., 2007) 20
  • 21. Pheromone  Intraspecific chemical substances that mediate communication between insects  Ectohormones  1959: Karlson and Butenandt  Silkworm moths 21
  • 22. Sex Pheromone  Pheromone released by one sex only and trigger behaviour patterns in the other sex that facilitate in mating  Female insect produce pheromone eg. Pink bollworm – gossyplure Helicoverpa armigera - helilure 22
  • 23. Female Sex Pheromone Bactrocera dorsalis Methyl eugenol 23
  • 24. Female Sex Pheromone contd… Bactrocera cucurbitae Cuelure 24
  • 25. Aggregation Pheromone  Pheromones which induce aggregation or congregation of insects for protection, reproduction and feeding  Coleoptera and Dictyoptera Cockroach – periplanone Phloem beetle - ipsenol 25
  • 26. Aggregation Pheromone contd…  Red palm weevil – Rhynchophorus ferrugenius  Ferrolure 26
  • 27. Alarm Pheromone  Chemical substance released by insects to warn members of same species about the presence or attack by an enemy eg. Aphids 27
  • 28. Trail Pheromone  Pheromones laid in form of intermittent or continuous lines on a soil substrate which trail followers perceive to reach their destination which could be a mate or food source eg. Ants 28
  • 29. Allelochemicals  Interspecific chemicals mediate the communication between two different species of insects  They are classified in to four categories 29
  • 30. Allomones  Chemical released by one insect that induce a response in another insect  Helpful to emitter eg. Sting glands in bees 30
  • 31. Kairomones  Chemical released by one insect that induce a response in another insect  Helpful to recipient eg. Heptanoic acid releases by potato tuber moth 31
  • 32. Synamone  Chemical released by one insect that induce a response in another insect  Helpful to emitter and receiver eg. Termites and protozoan's 32
  • 33. Apneumones  A chemical substance emitted by non living material that evoke the behavioural or physiological reaction adaptively for receiving organism eg. Oat meal oil 33
  • 34. Insect Attractants  Chemicals that cause insect to make oriented movements towards their source  Types of attractants are  Pheromone  Natural food lures 34
  • 35. Natural Food Lures  Chemicals present in plant and animal hosts that attract insect for feeding  Floral scents nectar feeding insect  Decomposing products 35
  • 36. Fish Meal Trap  Sorghum shoot fly is attracted to rotten fish meal 36
  • 38. Insect Repellants  An oriented movement of insects away from their source of food 38
  • 39. Insect Olfactory Sense contd...  Smell travels through air  Then they diffuse and are carried by an odorant binding protein (OBP)  Many odorants are received by insect olfactory sensillum and perceived by the olfactory receptor neurons (ORNs) (Galizia and Rossler, 2010) 39
  • 41. Sensilla Antennae Na+ , K+ channel Glomeruli 41
  • 42. Protocerebrum Projection neuron Response 42 Motor neuron
  • 43. Insect Olfactory Sense contd... OBP 43
  • 44. Insect Olfactory Sense contd...  Receptor neurons project an axon through the olfactory nerve into the primary olfactory centers – olfactory bulb 44
  • 45. Insect Olfactory Sense contd... Deutocerebrum  Have the olfactory lobe  Receives signal from glomeruli  Projection neurons will send the signal to protocerebrum 45
  • 46. Insect Olfactory Sense contd...  Projection neurons, connecting olfactory lobes to mushroom bodies and other parts of protocerebrum. 46
  • 47. Insect Olfactory Sense contd...  The physiological characteristics of the projection neurons vary  They respond only when the antennal receptors are simulated by a specific odour like a pheromone, attractant or repellent components  Others are simply ignored or not recognized 47
  • 48. Olfactometer  Specific reaction to olfactory stimuli to be verified by olfactometer responses 48
  • 49. Examples for insect repellence by bad odour 49
  • 50. Repelling mosquitoes by burning neem leaves along with cow dung 50 (Mandavgane et al., 2005)
  • 51. To repel honey robber, some stingless social bees erect walls of sticky resin in front of or around the nest entrance, while other species smear a repugnant liquid there 51
  • 52. Eucalyptus oil – pleasant smell in human, repellent effect on insects like mosquitoes and fleas 52 (Lett and Kraus, 1994)
  • 53. Cocoa leaves, eucalyptus oil, garlic extract, olive oil mixture of these ingredients will repell the ants, cockroach, mosquitoes and spiders 53
  • 54. Osmeterium, an orange, Y-shaped structure on the neck of caterpillar gives off a strong, unpleasant odour when it is threatened 54
  • 55. 55
  • 56. Conclusion  Odour perception is like visiting a library. Thousands of books are present in the library. Similarly there are a number of odorants present in the environment. All groups of insects have specific odours which they receive through specific glomeruli. 56
  • 57.  Only odour stimuli in the case of insects. They receive only those known to them  There is nothing like good or bad odour 57
  • 58. 58