Plasmid.pptx
•Download as PPTX, PDF•
0 likes•8 views
This ppt includes a brief description about plasmids, selectable markers, type of plasmid, size and copy no. and classification.
Report
Share
Report
Share
Recommended
Different types of vectors.pptx
Vectors are widely used in different cloning experiments. There are different types of vectors such as plasmids and bacteriophages.
A seminar topic on cloning vector by goutam
A seminar on cloning vectors was presented. Cloning vectors are DNA molecules that can replicate within host cells to produce multiple copies of recombinant DNA. They must contain an origin of replication, cloning sites, selectable markers, and marker genes. Common types of cloning vectors include plasmids, bacteriophages, cosmids, and phagemids. Plasmids are the most commonly used cloning vector as they can replicate autonomously in bacteria and accept DNA insert sizes up to 10kb.
Microbiology
This document summarizes key information about bacterial genomes and genetic elements:
- Bacterial genomes vary in size and can contain one or more replicons like chromosomes and plasmids. Most bacteria have a single circular chromosome but some have linear or multiple chromosomes.
- Plasmids are extra-chromosomal DNA that can replicate independently. They often contain genes for functions like antibiotic resistance and virulence. Conjugative plasmids can transfer between bacteria.
- Other mobile genetic elements like transposons and integrons can move resistance genes between replicons. Site-specific recombination and horizontal gene transfer through transformation, transduction, and conjugation allow for genetic exchange between bacteria.
Lecture_3-Vectors.pdf
The document discusses cloning vectors. It describes what a cloning vector is, including that it is a small piece of DNA that can stably maintain foreign DNA for cloning purposes. Common types of cloning vectors are described in detail, including plasmids, bacteriophages, cosmids, yeast artificial chromosomes, bacterial artificial chromosomes, and plant virus vectors. Key features of cloning vectors like origins of replication, antibiotic resistance genes, and cloning sites are also summarized.
Vectors in recombinant dna technology pBR322
The document discusses different types of vectors used in recombinant DNA technology, including plasmids and artificial vectors like pBR322. Plasmids are naturally occurring extrachromosomal DNA molecules that can autonomously replicate in bacterial cells. Important properties of vectors include being able to replicate inside host cells, having compatible restriction sites for inserting DNA, and containing selectable markers. The document then describes specific natural plasmids like ColE1 and artificial cloning vectors like pBR322, which is 4361 base pairs long and was constructed in a laboratory to act as a cloning vector.
Gene cloning
GENE CLONING
PLASMIDS
COSMIDS
cloning vectors,
cosmid properties,
gene cloning, plasmid properties,
properties,
quality of good vectors,
vectors pbr322 , pbr325, puc18, puc19, puc series cosmid col
Plasmids
Plasmids are small, circular pieces of extrachromosomal DNA found in bacteria and other organisms. They can replicate independently of the chromosomal DNA and often contain genes that provide advantages to the host cell like antibiotic resistance. Key features of plasmids include an origin of replication, antibiotic resistance genes, and mechanisms for horizontal transfer between organisms like conjugation. Plasmids are important tools in biotechnology as they can be used to clone genes and amplify DNA fragments.
Recommended
Different types of vectors.pptx
Vectors are widely used in different cloning experiments. There are different types of vectors such as plasmids and bacteriophages.
A seminar topic on cloning vector by goutam
A seminar on cloning vectors was presented. Cloning vectors are DNA molecules that can replicate within host cells to produce multiple copies of recombinant DNA. They must contain an origin of replication, cloning sites, selectable markers, and marker genes. Common types of cloning vectors include plasmids, bacteriophages, cosmids, and phagemids. Plasmids are the most commonly used cloning vector as they can replicate autonomously in bacteria and accept DNA insert sizes up to 10kb.
Microbiology
This document summarizes key information about bacterial genomes and genetic elements:
- Bacterial genomes vary in size and can contain one or more replicons like chromosomes and plasmids. Most bacteria have a single circular chromosome but some have linear or multiple chromosomes.
- Plasmids are extra-chromosomal DNA that can replicate independently. They often contain genes for functions like antibiotic resistance and virulence. Conjugative plasmids can transfer between bacteria.
- Other mobile genetic elements like transposons and integrons can move resistance genes between replicons. Site-specific recombination and horizontal gene transfer through transformation, transduction, and conjugation allow for genetic exchange between bacteria.
Lecture_3-Vectors.pdf
The document discusses cloning vectors. It describes what a cloning vector is, including that it is a small piece of DNA that can stably maintain foreign DNA for cloning purposes. Common types of cloning vectors are described in detail, including plasmids, bacteriophages, cosmids, yeast artificial chromosomes, bacterial artificial chromosomes, and plant virus vectors. Key features of cloning vectors like origins of replication, antibiotic resistance genes, and cloning sites are also summarized.
Vectors in recombinant dna technology pBR322
The document discusses different types of vectors used in recombinant DNA technology, including plasmids and artificial vectors like pBR322. Plasmids are naturally occurring extrachromosomal DNA molecules that can autonomously replicate in bacterial cells. Important properties of vectors include being able to replicate inside host cells, having compatible restriction sites for inserting DNA, and containing selectable markers. The document then describes specific natural plasmids like ColE1 and artificial cloning vectors like pBR322, which is 4361 base pairs long and was constructed in a laboratory to act as a cloning vector.
Gene cloning
GENE CLONING
PLASMIDS
COSMIDS
cloning vectors,
cosmid properties,
gene cloning, plasmid properties,
properties,
quality of good vectors,
vectors pbr322 , pbr325, puc18, puc19, puc series cosmid col
Plasmids
Plasmids are small, circular pieces of extrachromosomal DNA found in bacteria and other organisms. They can replicate independently of the chromosomal DNA and often contain genes that provide advantages to the host cell like antibiotic resistance. Key features of plasmids include an origin of replication, antibiotic resistance genes, and mechanisms for horizontal transfer between organisms like conjugation. Plasmids are important tools in biotechnology as they can be used to clone genes and amplify DNA fragments.
Plasmids
Plasmids are double-stranded DNA molecules that exist independently of the chromosome in organisms. They can replicate on their own and provide benefits to the host such as antibiotic resistance. Plasmids come in different types based on their ability to transfer horizontally or their function. Common plasmid types include F-plasmids, which allow for bacterial conjugation, and R-plasmids, which contain antibiotic resistance genes. Plasmids are also useful as vectors in biotechnology as they contain origins of replication, antibiotic resistance genes, and multiple cloning sites that allow for insertion and expression of foreign DNA.
Gene cloning
“The technique in which a DNA fragment carrying a gene inserted into the cloning vector, this vector is introduced in the living cell and subsequent propagation of this recombination DNA molecule into many copies is known as gene cloning.
Ge m1 3 types of vectors
Vectors are essential tools for genetic engineering that allow recombinant DNA to be introduced into and replicated in host organisms. Plasmids are the most commonly used bacterial cloning vectors. Plasmids are small, circular DNA molecules that can replicate independently of the bacterial chromosome. Important plasmid vectors include pBR322, which contains two antibiotic resistance genes for selection, and pUC19, which allows blue-white screening to identify recombinant clones through disruption of the lacZ gene. Plasmid vectors are useful for amplifying DNA inserts, producing recombinant proteins, and transferring genes for applications such as gene therapy.
Cloning Vectors (plasmid).pdf
Plasmids are circular, self-replicating DNA molecules that are commonly used as cloning vectors. Key properties of plasmids that make them useful for cloning include their ability to replicate independently of the bacterial chromosome, carry genes for antibiotic resistance or other selectable markers, and accept foreign DNA at specific restriction sites. Common plasmids used for cloning are pBR322, which has features like a high copy number and blue-white screening, and pUC plasmids, which have even higher copy numbers. Plasmids can shuttle DNA between bacterial and yeast cells, and RNA production plasmids contain promoters to transcribe cloned DNA into RNA for applications like Northern blotting.
Plasmid
This document provides an overview of plasmids. It defines plasmids as small, circular, extrachromosomal DNA molecules that can replicate independently in bacteria. Plasmids contain genes that provide benefits to bacteria like antibiotic resistance. They are transferred between bacteria through processes like transformation, transduction, and conjugation. Plasmids are classified based on their functions and are important tools in biotechnology as they allow cloning, protein production, and other applications.
Cloning vectors
This document discusses cloning vectors. It begins with a brief history of cloning vectors, noting that the first designed cloning vector was the plasmid pBR322 created in 1977. It then describes the key features of cloning vectors, including an origin of replication, cloning sites, selectable markers like antibiotic resistance genes, and reporter genes. Examples of different types of cloning vectors are also provided, such as plasmids, bacteriophages, cosmids, and artificial chromosomes that can be used in prokaryotes or eukaryotes. The document concludes by differentiating between cloning vectors and expression vectors.
cloning vectors.pptx Biotechnology Notes
A cloning vector is a small piece of DNA, such as a plasmid, virus, or artificial chromosome, that can accept foreign DNA and be used to clone that DNA and replicate it in a host cell. This document discusses the history and features of common cloning vectors like plasmids, bacteriophages, cosmids, and artificial chromosomes. It explains how vectors are chosen based on factors like insert size and used in molecular cloning by digesting DNA with restriction enzymes, ligating into the vector, transforming into host cells, and selecting for recombinant clones.
PLANT TRANSFORMATIVE VECTORS.pptx
Plant transformation vectors can be classified into cloning vectors, expression vectors, and integration vectors. Cloning vectors are small DNA molecules used to insert, store, and manipulate foreign DNA. Common cloning vectors include plasmids, bacteriophages, cosmids, and BACs/YACs. Expression vectors allow foreign DNA to be inserted and expressed in host cells. The Ti plasmid is often used as a plant expression vector due to genes that mediate DNA transfer to plant cells. Vector choice depends on desired DNA insert size, host system, and purpose of cloning/expression.
PLANT TRANSFORMATIVE VECTORS.pptx
Plant transformation, vectors, vectors classification, cloning vectors, types of cloning vectors, plasmid vectors, classification of plasmid vectors, pBR322, advantages and disadvantages of vectors, Bacteriophage vectors, phage Lambda, M13 vectors, cosmids, bacterial artificial chromosome, yeast artificial chromosome, shuttle vectors, expression vectors, Ti plasmid, its advantages and disadvantage, conclusion
Cloning vector
This document discusses cloning vectors, which are DNA molecules used to transport cloned DNA sequences between biological hosts. It defines a cloning vector as a small piece of DNA from a virus, plasmid, or cell that can maintain foreign DNA for cloning. The summary describes the key features of cloning vectors, including an origin of replication, cloning site, selectable marker, and optional reporter gene. It also lists common vector types like plasmids, bacteriophages, cosmids, and artificial chromosomes, and factors that determine the choice of vector, such as insert size.
Cloning vectors
Objectives:
After the end of the presentation we’ll know -
What is cloning vector?
Why cloning vector?
History
Features of a cloning vector
Types of cloning vector
Plasmid
Bacteriophage
Cosmid
Bacterial Artificial Chromosome (BAC)
Yeast Artificial Chromosome (BAC)
Human Artificial Chromosome (HAC)
Retroviral Vectors
What determines choice of vector?
Vector in molecular gene cloning
Cloning vector - The molecular analysis of DNA has been made possible by the cloning of DNA. The two molecules that are required for cloning are the DNA to be cloned and a cloning vector.
A cloning vector is a small piece of DNA taken from a virus, a plasmid or the cell of a higher organism, that can be stably maintained in an organism and into which a foreign DNA fragment can be inserted for cloning purposes.
Most vectors are genetically engineered.
The cloning vector is chosen according to the size and type of DNA to be cloned.
The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector, for example by treating the vector and the foreign DNA with a restriction enzyme and then ligating the fragments together.
After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use.
cloning vectors.pptx Biotechnology class
A cloning vector is a small piece of DNA, such as a plasmid, virus, or artificial chromosome, that can accept foreign DNA and be replicated within a host cell. The summary describes the main types of cloning vectors, including plasmids, bacteriophages, cosmids, BACs, YACs, and retroviral vectors. It also outlines some key features of cloning vectors like origins of replication, cloning sites, and selectable markers. The choice of vector depends on factors like the size of the DNA insert and cloning efficiency.
Bacterial Plasmids.pptx
The document discusses plasmids, which are small, circular DNA molecules that can replicate independently of the host cell's chromosome. Plasmids are commonly used by molecular biologists to clone and propagate DNA sequences. The document covers several topics about plasmids, including their natural function in bacteria, classification based on structure and function, modes of replication, and their use as genetic tools for cloning and manipulating DNA. It also provides steps for purifying plasmids from bacterial cells based on their unique physical properties of being supercoiled and able to quickly renature after denaturation.
DESIGN OF PLASMID VECTOR & PROKARYOTIC AND EUKARYOTIC VECTORS
Plasmid vectors are circular, self-replicating DNA molecules that are commonly used to clone DNA fragments in bacteria. The document discusses the key features of plasmid vectors including their origin of replication, selectable marker genes, and cloning sites. It also describes different types of plasmids such as F-plasmids, R-plasmids, and Ti-plasmids. Common plasmid vectors used in genetic engineering like pUC19, pBR322, and Ti-plasmids are also outlined. Finally, the applications of plasmids in genetic engineering for cloning genes and mass producing proteins are briefly mentioned.
Plasmid ppt.pptx
The document discusses properties of plasmids, which are self-replicating DNA elements found in prokaryotic and some eukaryotic cells. Key points include:
- Plasmids are usually circular DNA molecules that can replicate independently of the host cell's chromosome.
- They carry genes that allow for their own replication and can also carry genes that affect host cell properties.
- Plasmids can exist in varying copy numbers within a host cell and some are capable of transferring between bacterial cells.
- Common plasmid functions include antibiotic resistance, virulence factors for pathogenicity, and mediating tumor formation in plants by Agrobacterium.
Vectors plasmid, phage and cosmids
Cloning vectors are DNA molecules that are used to transport cloned DNA sequences between biological hosts. Common features of cloning vectors include an origin of replication, a selectable marker, unique restriction sites, and minimal nonessential DNA. Plasmid vectors are commonly used cloning vectors that can clone inserts up to 10kb in size. They contain an origin of replication, antibiotic resistance gene, and multiple cloning site. Cosmid vectors are plasmid vectors that contain lambda phage cos sites, allowing them to package DNA into phage particles and accept larger 45kb inserts for cloning. Different vector types are chosen based on the size and type of DNA being cloned.
Plasmid
Plasmids are small, circular DNA molecules that are separate from the bacterial chromosome and can replicate independently. They were first described by American molecular biologist Joshua Lederberg in 1952. Plasmids often contain genes that provide bacteria with functions not necessary for survival, such as antibiotic resistance or virulence factors. They are commonly used as cloning vectors in genetic engineering to generate copies of genes of interest in bacteria. Plasmids have an origin of replication, selectable marker gene, and cloning site that allow them to be used to replicate, select for, and clone DNA fragments in bacteria.
PLASMID - TYPES & ITS PROPERTIES.pptx
• Plasmids are extra-chromosomal genetic elements that replicate independently of the host chromosome.
• They are small, circular (some are linear), double-stranded DNA molecules that exist in bacterial cells and in some eukaryotes.
Vectors in Recombenant DNA technology
Vectors are DNA molecules that can carry foreign DNA into host cells. There are two main types of vectors - expression vectors, which are used to produce proteins, and cloning vectors, which are used to replicate and clone DNA fragments. Common cloning vectors include plasmids, cosmids, phage vectors like lambda, and bacterial and yeast artificial chromosomes. Plasmids are small, circular DNA molecules that are commonly used to clone DNA fragments up to 12kb in size. Cosmids and phage vectors like lambda can carry larger fragments up to 45kb and 20kb respectively. Bacterial and yeast artificial chromosomes can carry even larger fragments up to 100kb and 1mb respectively but are more difficult to work with. All vectors require key
Leveraging Generative AI to Drive Nonprofit Innovation
In this webinar, participants learned how to utilize Generative AI to streamline operations and elevate member engagement. Amazon Web Service experts provided a customer specific use cases and dived into low/no-code tools that are quick and easy to deploy through Amazon Web Service (AWS.)
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
-------------------------------------------------------------------------------
Find out more about ISO training and certification services
Training: ISO/IEC 27001 Information Security Management System - EN | PECB
ISO/IEC 42001 Artificial Intelligence Management System - EN | PECB
General Data Protection Regulation (GDPR) - Training Courses - EN | PECB
Webinars: https://pecb.com/webinars
Article: https://pecb.com/article
-------------------------------------------------------------------------------
For more information about PECB:
Website: https://pecb.com/
LinkedIn: https://www.linkedin.com/company/pecb/
Facebook: https://www.facebook.com/PECBInternational/
Slideshare: http://www.slideshare.net/PECBCERTIFICATION
More Related Content
Similar to Plasmid.pptx
Plasmids
Plasmids are double-stranded DNA molecules that exist independently of the chromosome in organisms. They can replicate on their own and provide benefits to the host such as antibiotic resistance. Plasmids come in different types based on their ability to transfer horizontally or their function. Common plasmid types include F-plasmids, which allow for bacterial conjugation, and R-plasmids, which contain antibiotic resistance genes. Plasmids are also useful as vectors in biotechnology as they contain origins of replication, antibiotic resistance genes, and multiple cloning sites that allow for insertion and expression of foreign DNA.
Gene cloning
“The technique in which a DNA fragment carrying a gene inserted into the cloning vector, this vector is introduced in the living cell and subsequent propagation of this recombination DNA molecule into many copies is known as gene cloning.
Ge m1 3 types of vectors
Vectors are essential tools for genetic engineering that allow recombinant DNA to be introduced into and replicated in host organisms. Plasmids are the most commonly used bacterial cloning vectors. Plasmids are small, circular DNA molecules that can replicate independently of the bacterial chromosome. Important plasmid vectors include pBR322, which contains two antibiotic resistance genes for selection, and pUC19, which allows blue-white screening to identify recombinant clones through disruption of the lacZ gene. Plasmid vectors are useful for amplifying DNA inserts, producing recombinant proteins, and transferring genes for applications such as gene therapy.
Cloning Vectors (plasmid).pdf
Plasmids are circular, self-replicating DNA molecules that are commonly used as cloning vectors. Key properties of plasmids that make them useful for cloning include their ability to replicate independently of the bacterial chromosome, carry genes for antibiotic resistance or other selectable markers, and accept foreign DNA at specific restriction sites. Common plasmids used for cloning are pBR322, which has features like a high copy number and blue-white screening, and pUC plasmids, which have even higher copy numbers. Plasmids can shuttle DNA between bacterial and yeast cells, and RNA production plasmids contain promoters to transcribe cloned DNA into RNA for applications like Northern blotting.
Plasmid
This document provides an overview of plasmids. It defines plasmids as small, circular, extrachromosomal DNA molecules that can replicate independently in bacteria. Plasmids contain genes that provide benefits to bacteria like antibiotic resistance. They are transferred between bacteria through processes like transformation, transduction, and conjugation. Plasmids are classified based on their functions and are important tools in biotechnology as they allow cloning, protein production, and other applications.
Cloning vectors
This document discusses cloning vectors. It begins with a brief history of cloning vectors, noting that the first designed cloning vector was the plasmid pBR322 created in 1977. It then describes the key features of cloning vectors, including an origin of replication, cloning sites, selectable markers like antibiotic resistance genes, and reporter genes. Examples of different types of cloning vectors are also provided, such as plasmids, bacteriophages, cosmids, and artificial chromosomes that can be used in prokaryotes or eukaryotes. The document concludes by differentiating between cloning vectors and expression vectors.
cloning vectors.pptx Biotechnology Notes
A cloning vector is a small piece of DNA, such as a plasmid, virus, or artificial chromosome, that can accept foreign DNA and be used to clone that DNA and replicate it in a host cell. This document discusses the history and features of common cloning vectors like plasmids, bacteriophages, cosmids, and artificial chromosomes. It explains how vectors are chosen based on factors like insert size and used in molecular cloning by digesting DNA with restriction enzymes, ligating into the vector, transforming into host cells, and selecting for recombinant clones.
PLANT TRANSFORMATIVE VECTORS.pptx
Plant transformation vectors can be classified into cloning vectors, expression vectors, and integration vectors. Cloning vectors are small DNA molecules used to insert, store, and manipulate foreign DNA. Common cloning vectors include plasmids, bacteriophages, cosmids, and BACs/YACs. Expression vectors allow foreign DNA to be inserted and expressed in host cells. The Ti plasmid is often used as a plant expression vector due to genes that mediate DNA transfer to plant cells. Vector choice depends on desired DNA insert size, host system, and purpose of cloning/expression.
PLANT TRANSFORMATIVE VECTORS.pptx
Plant transformation, vectors, vectors classification, cloning vectors, types of cloning vectors, plasmid vectors, classification of plasmid vectors, pBR322, advantages and disadvantages of vectors, Bacteriophage vectors, phage Lambda, M13 vectors, cosmids, bacterial artificial chromosome, yeast artificial chromosome, shuttle vectors, expression vectors, Ti plasmid, its advantages and disadvantage, conclusion
Cloning vector
This document discusses cloning vectors, which are DNA molecules used to transport cloned DNA sequences between biological hosts. It defines a cloning vector as a small piece of DNA from a virus, plasmid, or cell that can maintain foreign DNA for cloning. The summary describes the key features of cloning vectors, including an origin of replication, cloning site, selectable marker, and optional reporter gene. It also lists common vector types like plasmids, bacteriophages, cosmids, and artificial chromosomes, and factors that determine the choice of vector, such as insert size.
Cloning vectors
Objectives:
After the end of the presentation we’ll know -
What is cloning vector?
Why cloning vector?
History
Features of a cloning vector
Types of cloning vector
Plasmid
Bacteriophage
Cosmid
Bacterial Artificial Chromosome (BAC)
Yeast Artificial Chromosome (BAC)
Human Artificial Chromosome (HAC)
Retroviral Vectors
What determines choice of vector?
Vector in molecular gene cloning
Cloning vector - The molecular analysis of DNA has been made possible by the cloning of DNA. The two molecules that are required for cloning are the DNA to be cloned and a cloning vector.
A cloning vector is a small piece of DNA taken from a virus, a plasmid or the cell of a higher organism, that can be stably maintained in an organism and into which a foreign DNA fragment can be inserted for cloning purposes.
Most vectors are genetically engineered.
The cloning vector is chosen according to the size and type of DNA to be cloned.
The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector, for example by treating the vector and the foreign DNA with a restriction enzyme and then ligating the fragments together.
After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use.
cloning vectors.pptx Biotechnology class
A cloning vector is a small piece of DNA, such as a plasmid, virus, or artificial chromosome, that can accept foreign DNA and be replicated within a host cell. The summary describes the main types of cloning vectors, including plasmids, bacteriophages, cosmids, BACs, YACs, and retroviral vectors. It also outlines some key features of cloning vectors like origins of replication, cloning sites, and selectable markers. The choice of vector depends on factors like the size of the DNA insert and cloning efficiency.
Bacterial Plasmids.pptx
The document discusses plasmids, which are small, circular DNA molecules that can replicate independently of the host cell's chromosome. Plasmids are commonly used by molecular biologists to clone and propagate DNA sequences. The document covers several topics about plasmids, including their natural function in bacteria, classification based on structure and function, modes of replication, and their use as genetic tools for cloning and manipulating DNA. It also provides steps for purifying plasmids from bacterial cells based on their unique physical properties of being supercoiled and able to quickly renature after denaturation.
DESIGN OF PLASMID VECTOR & PROKARYOTIC AND EUKARYOTIC VECTORS
Plasmid vectors are circular, self-replicating DNA molecules that are commonly used to clone DNA fragments in bacteria. The document discusses the key features of plasmid vectors including their origin of replication, selectable marker genes, and cloning sites. It also describes different types of plasmids such as F-plasmids, R-plasmids, and Ti-plasmids. Common plasmid vectors used in genetic engineering like pUC19, pBR322, and Ti-plasmids are also outlined. Finally, the applications of plasmids in genetic engineering for cloning genes and mass producing proteins are briefly mentioned.
Plasmid ppt.pptx
The document discusses properties of plasmids, which are self-replicating DNA elements found in prokaryotic and some eukaryotic cells. Key points include:
- Plasmids are usually circular DNA molecules that can replicate independently of the host cell's chromosome.
- They carry genes that allow for their own replication and can also carry genes that affect host cell properties.
- Plasmids can exist in varying copy numbers within a host cell and some are capable of transferring between bacterial cells.
- Common plasmid functions include antibiotic resistance, virulence factors for pathogenicity, and mediating tumor formation in plants by Agrobacterium.
Vectors plasmid, phage and cosmids
Cloning vectors are DNA molecules that are used to transport cloned DNA sequences between biological hosts. Common features of cloning vectors include an origin of replication, a selectable marker, unique restriction sites, and minimal nonessential DNA. Plasmid vectors are commonly used cloning vectors that can clone inserts up to 10kb in size. They contain an origin of replication, antibiotic resistance gene, and multiple cloning site. Cosmid vectors are plasmid vectors that contain lambda phage cos sites, allowing them to package DNA into phage particles and accept larger 45kb inserts for cloning. Different vector types are chosen based on the size and type of DNA being cloned.
Plasmid
Plasmids are small, circular DNA molecules that are separate from the bacterial chromosome and can replicate independently. They were first described by American molecular biologist Joshua Lederberg in 1952. Plasmids often contain genes that provide bacteria with functions not necessary for survival, such as antibiotic resistance or virulence factors. They are commonly used as cloning vectors in genetic engineering to generate copies of genes of interest in bacteria. Plasmids have an origin of replication, selectable marker gene, and cloning site that allow them to be used to replicate, select for, and clone DNA fragments in bacteria.
PLASMID - TYPES & ITS PROPERTIES.pptx
• Plasmids are extra-chromosomal genetic elements that replicate independently of the host chromosome.
• They are small, circular (some are linear), double-stranded DNA molecules that exist in bacterial cells and in some eukaryotes.
Vectors in Recombenant DNA technology
Vectors are DNA molecules that can carry foreign DNA into host cells. There are two main types of vectors - expression vectors, which are used to produce proteins, and cloning vectors, which are used to replicate and clone DNA fragments. Common cloning vectors include plasmids, cosmids, phage vectors like lambda, and bacterial and yeast artificial chromosomes. Plasmids are small, circular DNA molecules that are commonly used to clone DNA fragments up to 12kb in size. Cosmids and phage vectors like lambda can carry larger fragments up to 45kb and 20kb respectively. Bacterial and yeast artificial chromosomes can carry even larger fragments up to 100kb and 1mb respectively but are more difficult to work with. All vectors require key
Similar to Plasmid.pptx (20)
DESIGN OF PLASMID VECTOR & PROKARYOTIC AND EUKARYOTIC VECTORS
DESIGN OF PLASMID VECTOR & PROKARYOTIC AND EUKARYOTIC VECTORS
Recently uploaded
Leveraging Generative AI to Drive Nonprofit Innovation
In this webinar, participants learned how to utilize Generative AI to streamline operations and elevate member engagement. Amazon Web Service experts provided a customer specific use cases and dived into low/no-code tools that are quick and easy to deploy through Amazon Web Service (AWS.)
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
-------------------------------------------------------------------------------
Find out more about ISO training and certification services
Training: ISO/IEC 27001 Information Security Management System - EN | PECB
ISO/IEC 42001 Artificial Intelligence Management System - EN | PECB
General Data Protection Regulation (GDPR) - Training Courses - EN | PECB
Webinars: https://pecb.com/webinars
Article: https://pecb.com/article
-------------------------------------------------------------------------------
For more information about PECB:
Website: https://pecb.com/
LinkedIn: https://www.linkedin.com/company/pecb/
Facebook: https://www.facebook.com/PECBInternational/
Slideshare: http://www.slideshare.net/PECBCERTIFICATION
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
Physical pharmaceutics notes for B.pharm students
Hindi varnamala | hindi alphabet PPT.pdf
हिंदी वर्णमाला पीपीटी, hindi alphabet PPT presentation, hindi varnamala PPT, Hindi Varnamala pdf, हिंदी स्वर, हिंदी व्यंजन, sikhiye hindi varnmala, dr. mulla adam ali, hindi language and literature, hindi alphabet with drawing, hindi alphabet pdf, hindi varnamala for childrens, hindi language, hindi varnamala practice for kids, https://www.drmullaadamali.com
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Aberdeen
Walmart Business+ and Spark Good for Nonprofits.pdf
"Learn about all the ways Walmart supports nonprofit organizations.
You will hear from Liz Willett, the Head of Nonprofits, and hear about what Walmart is doing to help nonprofits, including Walmart Business and Spark Good. Walmart Business+ is a new offer for nonprofits that offers discounts and also streamlines nonprofits order and expense tracking, saving time and money.
The webinar may also give some examples on how nonprofits can best leverage Walmart Business+.
The event will cover the following::
Walmart Business + (https://business.walmart.com/plus) is a new shopping experience for nonprofits, schools, and local business customers that connects an exclusive online shopping experience to stores. Benefits include free delivery and shipping, a 'Spend Analytics” feature, special discounts, deals and tax-exempt shopping.
Special TechSoup offer for a free 180 days membership, and up to $150 in discounts on eligible orders.
Spark Good (walmart.com/sparkgood) is a charitable platform that enables nonprofits to receive donations directly from customers and associates.
Answers about how you can do more with Walmart!"
How to deliver Powerpoint Presentations.pptx
"How to make and deliver dynamic presentations by making it more interactive to captivate your audience attention"
BÀI TẬP BỔ TRỢ TIẾNG ANH 8 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2023-2024 (CÓ FI...
BÀI TẬP BỔ TRỢ TIẾNG ANH 8 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2023-2024 (CÓ FI...Nguyen Thanh Tu Collection
https://app.box.com/s/y977uz6bpd3af4qsebv7r9b7s21935vdThe History of Stoke Newington Street Names
Presented at the Stoke Newington Literary Festival on 9th June 2024
www.StokeNewingtonHistory.com
Bed Making ( Introduction, Purpose, Types, Articles, Scientific principles, N...
Topic : Bed making
Subject : Nursing Foundation
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UP
This Dissertation explores the particular circumstances of Mirzapur, a region located in the
core of India. Mirzapur, with its varied terrains and abundant biodiversity, offers an optimal
environment for investigating the changes in vegetation cover dynamics. Our study utilizes
advanced technologies such as GIS (Geographic Information Systems) and Remote sensing to
analyze the transformations that have taken place over the course of a decade.
The complex relationship between human activities and the environment has been the focus
of extensive research and worry. As the global community grapples with swift urbanization,
population expansion, and economic progress, the effects on natural ecosystems are becoming
more evident. A crucial element of this impact is the alteration of vegetation cover, which plays a
significant role in maintaining the ecological equilibrium of our planet.Land serves as the foundation for all human activities and provides the necessary materials for
these activities. As the most crucial natural resource, its utilization by humans results in different
'Land uses,' which are determined by both human activities and the physical characteristics of the
land.
The utilization of land is impacted by human needs and environmental factors. In countries
like India, rapid population growth and the emphasis on extensive resource exploitation can lead
to significant land degradation, adversely affecting the region's land cover.
Therefore, human intervention has significantly influenced land use patterns over many
centuries, evolving its structure over time and space. In the present era, these changes have
accelerated due to factors such as agriculture and urbanization. Information regarding land use and
cover is essential for various planning and management tasks related to the Earth's surface,
providing crucial environmental data for scientific, resource management, policy purposes, and
diverse human activities.
Accurate understanding of land use and cover is imperative for the development planning
of any area. Consequently, a wide range of professionals, including earth system scientists, land
and water managers, and urban planners, are interested in obtaining data on land use and cover
changes, conversion trends, and other related patterns. The spatial dimensions of land use and
cover support policymakers and scientists in making well-informed decisions, as alterations in
these patterns indicate shifts in economic and social conditions. Monitoring such changes with the
help of Advanced technologies like Remote Sensing and Geographic Information Systems is
crucial for coordinated efforts across different administrative levels. Advanced technologies like
Remote Sensing and Geographic Information Systems
9
Changes in vegetation cover refer to variations in the distribution, composition, and overall
structure of plant communities across different temporal and spatial scales. These changes can
occur natural.
Traditional Musical Instruments of Arunachal Pradesh and Uttar Pradesh - RAYH...
Traditional Musical Instruments of Arunachal Pradesh and Uttar Pradesh
Advanced Java[Extra Concepts, Not Difficult].docx
This is part 2 of my Java Learning Journey. This contains Hashing, ArrayList, LinkedList, Date and Time Classes, Calendar Class and more.
clinical examination of hip joint (1).pdf
described clinical examination all orthopeadic conditions .
Recently uploaded (20)
Leveraging Generative AI to Drive Nonprofit Innovation
Leveraging Generative AI to Drive Nonprofit Innovation
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
Walmart Business+ and Spark Good for Nonprofits.pdf
Walmart Business+ and Spark Good for Nonprofits.pdf
B. Ed Syllabus for babasaheb ambedkar education university.pdf
B. Ed Syllabus for babasaheb ambedkar education university.pdf
BÀI TẬP BỔ TRỢ TIẾNG ANH 8 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2023-2024 (CÓ FI...
BÀI TẬP BỔ TRỢ TIẾNG ANH 8 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2023-2024 (CÓ FI...
Bed Making ( Introduction, Purpose, Types, Articles, Scientific principles, N...
Bed Making ( Introduction, Purpose, Types, Articles, Scientific principles, N...
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UP
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UP
Traditional Musical Instruments of Arunachal Pradesh and Uttar Pradesh - RAYH...
Traditional Musical Instruments of Arunachal Pradesh and Uttar Pradesh - RAYH...
Plasmid.pptx
- 2. Content ● Vectors ● Plasmids ● Selectable markers ● Types of Plasmids ● Size and Copy number ● Conjugation and compatibility ● Plasmid Classification
- 3. Vectors ● Vehicle to transport genetics material from one organism to another. ● Examples:- ○ Plasmid ○ Cosmid ○ Lambda phages
- 4. Plasmids ● Circular piece of autonomously replicating DNA. ● Independent existence. ● Origin of replication, able to multiply independently. ● May use host’s cell replicative enzymes .
- 5. Selectable markers ● Carry genes for some useful characteristics, may be used as a selectable marker. ● Introduce into a cell of bacterium /culture, confers trait for artificial selection. ● Provide antibiotic resistance to the bacterium ● Eg. chloramphenicol or ampicillin
- 6. Types of Plasmid Types of Plasmids Non-integrative Plasmid Episome
- 7. Size and Copy number ● Size ○ Important for cloning. ○ >10 kb is desirable. ○ Ranges from 1.0 kb to 250 kb. ● Copy number ○ Should be present in multiple copies. ○ Stringent- 1 or 2 per cell ○ Relaxed- 50 or more
- 10. Plasmid Classification 1. Fertility or F plasmid:- carry tra gene (F-plasmid of E.coli) 2. Resistance or R plasmid:- carry genes for antibiotic resistance (RP4) 3. Col plasmid:- code for colicin, proteins that kill other bacteria (ColE1 of E.coli) 4. Degenerative plasmid:- allow host bacterium to metabolize unusual molecule (TOL of Pseudomonas Putida) 5. Virulence plasmid:- confer pathogenicity on host bacterium (Ti plasmid of Agrobacterium tumefaciens)
- 11. References ● T.A. Brown, GENE CLONING & DNA ANALYSIS, 7th Edition ● https://slideplayer.com/slide/5846852/
- 12. THANK YOU