Recombinant DNA technology

1. A SEMINAR TOPIC
ON CLONING
VECTOR
Presented by:GOUTAM KUMAR BEHERA
BOTANY

2. INTRODUCTION
● A cloning vector is a DNA molecule in which foreign DNA can be inserted or integrated & which is further capable of
replicating within Host cell to produce multiple clones of recombinant DNA.
● Example :- plasmid, phage or virus

3. CHARACTERISTICS
● Origin of replication
● Cloning sites
● Selectable markers
● Marker genes

4. TYPES OF CLONING VECTOR :
1. Plasmid as a vector
2. Bacteriophase as a vector
3. Cosmid as a vector
4. Phagemid as a vector

5. Plasmid as a vector
● Plasmid is an autonomously replicating circular double stranded extra chromosomal DNA & can
replicate indipendently.
● They are most commonly found in bacteria, sometime they are present in arachaea & eukaryotic
organism.
● The size of plasmid varies from 1 to over 200 kb.
● Most general plasmid that are used to clone DNA insert upto 10kb in size.
● Some examples of plasmid vector are - pBR322 , pUC 18 & pUC 19 etc.

6. pBR322 as vector

12. COSMID AS A VECTOR
● Cosmid are the plasmid that incorporates a segment of bacteriophage lambda DNA that has the cohesive end site
(cos) which contains the elements required for packaging of DNA into lambda particles.
● It is normally used to clone large segment of DNA between 25&45.
● They can replicate as plasmid if they have suitable origin of replication.
● They can also be packed in phage capsids, which allows the foreign gene to be transferred into cell by transduction.