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-:CONTENTS:-
• INTRODUCTION
• CHARACTERISTICS
• CLASSES OF PLANT HORMONES
a)Auxin
b)Gibberellin
c)Cytokinin
d)Ethylene
e)Absisic acid
• OTHER KNOWN HORMONES
• POTENTIAL MEDICALAPPLICATION
• CONCLUSION
• REFERENCES
INTRODUCTION:-
• Plant hormones are also known as phytohormones.
• Plant hormones are chemicals that regulate plant growth.
• Hormones also determine the formation of
flowers,stems,leaves,the shedding of leaves, and the
development and ripening of fruit.
• Hormones are vital to plant growth, and lacking them, palnts
would be mostly a mass of undifferentiated cells.so they are
also known as growth factors or growth hormones.
• The term “PHYTOHORMONES” was coined by ‘THIMANN’ in
1948.
CHARACTERISTICS:-
• The word hormone is derived from Greek , meaning set in
motion. plant hormones affect gene expression and
transcription levels , cellular division and growth.
• They are naturally produced within plants , though very
similar chemicals are produced by fungi and bacteria , that
can also affect plant growth.
• Plant hormones are not nutrients , but chemicals that in small
amount promote and influence the growth, development ,
and differentiation of cells and tissues.
CLASSES OF PLANT HORMONES:-
 In general , it is accepted there are five major classes of plants
hormones , some of which are made up of many different
chemicals that can vary in structure from one plant to the
next.
 The five major classes are :-
a)AUXIN
b)GIBBERELLIN
c)CYTOKININ
d)ETHYLINE
e)ABSISIC ACID
Auxin :-
• Discovered in 1881 by CHARLES AND FRANCIS DARWIN.
• They reported experiments on the response of growing plants
to light.
 Grass seedlings do not bend if the tip is covered with a light
proof cap.
 They do not bend when a collar is placed below the tip.
 30 year later, PETER BOYSEN JENSEN AND ARPAD PAAL
demonstrated that the influence was actually a chemical.
AUXIN:-
 PRODUCTION
• Shoot tips
• Developing seeds
 SOME KNOWN ACTION
• Establish of polarity of root –shoot axis during embryogenesis.
• Cell elongation.
• Cell differentiation.
• Apical dominance.
• Lateral root formation & adventitious root formation.
• Fruit formation
Under normal conditions,
shoot tips bend towards
the light
Without light on the
tip, no bending
When not at tip, collar
doesn’t prevent
bending
Conclusion: Light is sensed at the tip, but response not at tip
New hypothesis: A substance or chemical is transported
Auxin later isolated from shoot tips and established to be involved in
cell elongation
Drawings depicting seedlings of Zea (Gramineae family)
Darwins’ (Charles and son) experiment
9
High auxin
concentration
Low auxin
concentration
Drawings depicting Coleus (Lamiaceae family)
Evidence for the role of auxin
in apical dominance
Auxin
• In 1926, Frits Went performed an experiment that
explained all of the previous results
• He named the chemical messenger auxin
• It accumulated on the side of an oat seedling away
from light
• Promoted these cells to grow faster than those on
the lighted side
• Cell elongation causes the plant to bend towards
light
11
12
• Chemical enhanced rather than retarded cell elongation
• Frits Went named the substance that he had discovered
auxin
13
How Auxin Works
• Indoleacetic acid (IAA) is the most common
natural auxin
• Probably synthesized from tryptophan
14
Synthetic Auxins
• Naphthalene acetic acid (NAA) and
indolebutyric acid (IBA) have many uses in
agriculture and horticulture
• Prevent abscission in apples and berries
• Promote flowering and fruiting in pineapples
• 2,4-dichlorophenoxyacetic acid (2,4-D) is a
herbicide commonly used to kill weeds
15
Gibberellins
• Named after the fungus Gibberella fujikuroi
which causes rice plants to grow very tall
• Gibberellins belong to a large class of over 100
naturally occurring plant hormones
– All are acidic and abbreviated GA
– Have important effects on stem elongation
• Enhanced if auxin present
16
• Adding gibberellins to certain dwarf mutants
restores normal growth and development
17
• GA is used as a signal from the embryo that
turns on transcription of genes encoding
hydrolytic enzymes in the aleurone layer
• When GA binds to its receptor, it frees GA-
dependent transcription factors from a
repressor
• These transcription factors can now directly
affect gene expression
18
Gibberellins
• Hasten seed germination
• Used commercially to extend internode length
in grapes
– Result is larger grapes
19
Cytokinins
• Plant hormone that, in combination with
auxin, stimulates cell division and
differentiation
20
Synthetic cytokinins
• Produced in the root apical meristems and
developing fruits
• In all plants, cytokinins, working with other
hormones, seem to regulate growth patterns
• Promote the growth of lateral buds into
branches
• Inhibit the formation of lateral roots
– Auxin promotes their formation
21
22
Cytokinins
• Promote the synthesis or
activation of cytokinesis proteins
• Also function as antiaging
hormones
• Agrobacterium inserts genes that
increase rate of cytokinin and
auxin production
– Causes massive cell division
– Formation of crown gall tumor
23
• Plant tissue can
form shoots,
roots, or an
undifferentiated
mass depending
on the relative
amounts of auxin
and cytokinin
24
Ethylene
• Gaseous hydrocarbon (H2C―CH2)
• Auxin stimulates ethylene production in the tissues
around the lateral bud and thus retards their growth
• Ethylene also suppresses stem and root elongation
• Major role in fruit development – hastens ripening
– Transgenic tomato plant can’t make ethylene
– Shipped without ripening and rotting
25
Abscisic Acid
• Synthesized mainly in mature green leaves,
fruits, and root caps
• Little evidence that this hormone plays a role
in abscission
• Induces formation of dormant winter buds
• Counteracts gibberellins by suppressing bud
growth and elongation
• Counteracts auxin by promoting senescence
26
Abscisic Acid
• Necessary for dormancy in seeds
– Prevents precocious germination called vivipary
• Important in the opening and closing of
stomata
27
28
Other known hormones:-
• Other identified plant growth regulators
include-
a)BRASSINOSTEROID:-
• First discovered in the pollen of brassica spp.
• Are structurally simillar to steroid hormones.
Brassinosteroids
• Functional overlap with other plant hormones,
especially auxins and gibberellins
• Broad spectrum of physiological effects
– Elongation, cell division, stem bending, vascular
tissue development, delayed senescence,
membrane polarization and reproductive
development
31
Salicylic acid:-
• Activate genes in some plants that produce chemicals
that aid in the defense against pathogenic invader.
Jasmonates :-
• Are produced from fatty acids & seems to promote
the production of defense proteins that are used to
fend off invading organisms.
• Also have a role in seed germination.
plant peptide hormones:-
 Involved in cell to cell signaling.
 Roles in plant growth &development , including defense
mechanism.
Polyamines:-
 Are strongly basic molecule with low molecular weight that
have been found in all organism studied thus far.
Nitric oxide:-
 Serves as signals in hormonal &defense response.
 E.G-nitrogen fixation , stomata closure , germination , cell
death.
Karrikins:-
• Not plant hormones because they are not made by plants, but
are a group of plant growth regulator found in the smoke of
burning plant materials that have the ability to stimulates the
germination of seeds.
Strigolactones :-
 Implicated in the inhibition of shoot branching.
Triacontanol :-
 A fatty alcohol that acts as a growth stimulant, especially
initiating new basal breaks in the rose family.
 It is found in ALFALFA (Lucerne),BEE’S WAX.
POTENTIAL MEDICAL APPLICATION:-
 Plant stress hormones activate cellular response, including
cell death to diverse stress situation in plants.
 Researchers have found that some plant stress hormone
share the ability to adversely affect human cancer cells.
 E.G:-sodium salicylate has been found to suppress
proliferation of LYMPHOBLASTIC LEUKEMIA,PROSTATE
&MELANOMA human cancer cells.
 Methyl jasmonate has been found to induce cell death in a
number of cancer cell lines.

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Plant growth hormone

  • 1. -:CONTENTS:- • INTRODUCTION • CHARACTERISTICS • CLASSES OF PLANT HORMONES a)Auxin b)Gibberellin c)Cytokinin d)Ethylene e)Absisic acid • OTHER KNOWN HORMONES • POTENTIAL MEDICALAPPLICATION • CONCLUSION • REFERENCES
  • 2. INTRODUCTION:- • Plant hormones are also known as phytohormones. • Plant hormones are chemicals that regulate plant growth. • Hormones also determine the formation of flowers,stems,leaves,the shedding of leaves, and the development and ripening of fruit. • Hormones are vital to plant growth, and lacking them, palnts would be mostly a mass of undifferentiated cells.so they are also known as growth factors or growth hormones. • The term “PHYTOHORMONES” was coined by ‘THIMANN’ in 1948.
  • 3. CHARACTERISTICS:- • The word hormone is derived from Greek , meaning set in motion. plant hormones affect gene expression and transcription levels , cellular division and growth. • They are naturally produced within plants , though very similar chemicals are produced by fungi and bacteria , that can also affect plant growth. • Plant hormones are not nutrients , but chemicals that in small amount promote and influence the growth, development , and differentiation of cells and tissues.
  • 4. CLASSES OF PLANT HORMONES:-  In general , it is accepted there are five major classes of plants hormones , some of which are made up of many different chemicals that can vary in structure from one plant to the next.  The five major classes are :- a)AUXIN b)GIBBERELLIN c)CYTOKININ d)ETHYLINE e)ABSISIC ACID
  • 5. Auxin :- • Discovered in 1881 by CHARLES AND FRANCIS DARWIN. • They reported experiments on the response of growing plants to light.  Grass seedlings do not bend if the tip is covered with a light proof cap.  They do not bend when a collar is placed below the tip.  30 year later, PETER BOYSEN JENSEN AND ARPAD PAAL demonstrated that the influence was actually a chemical.
  • 6. AUXIN:-  PRODUCTION • Shoot tips • Developing seeds  SOME KNOWN ACTION • Establish of polarity of root –shoot axis during embryogenesis. • Cell elongation. • Cell differentiation. • Apical dominance. • Lateral root formation & adventitious root formation. • Fruit formation
  • 7. Under normal conditions, shoot tips bend towards the light Without light on the tip, no bending When not at tip, collar doesn’t prevent bending Conclusion: Light is sensed at the tip, but response not at tip New hypothesis: A substance or chemical is transported Auxin later isolated from shoot tips and established to be involved in cell elongation Drawings depicting seedlings of Zea (Gramineae family) Darwins’ (Charles and son) experiment
  • 8. 9
  • 9. High auxin concentration Low auxin concentration Drawings depicting Coleus (Lamiaceae family) Evidence for the role of auxin in apical dominance
  • 10. Auxin • In 1926, Frits Went performed an experiment that explained all of the previous results • He named the chemical messenger auxin • It accumulated on the side of an oat seedling away from light • Promoted these cells to grow faster than those on the lighted side • Cell elongation causes the plant to bend towards light 11
  • 11. 12
  • 12. • Chemical enhanced rather than retarded cell elongation • Frits Went named the substance that he had discovered auxin 13
  • 13. How Auxin Works • Indoleacetic acid (IAA) is the most common natural auxin • Probably synthesized from tryptophan 14
  • 14. Synthetic Auxins • Naphthalene acetic acid (NAA) and indolebutyric acid (IBA) have many uses in agriculture and horticulture • Prevent abscission in apples and berries • Promote flowering and fruiting in pineapples • 2,4-dichlorophenoxyacetic acid (2,4-D) is a herbicide commonly used to kill weeds 15
  • 15. Gibberellins • Named after the fungus Gibberella fujikuroi which causes rice plants to grow very tall • Gibberellins belong to a large class of over 100 naturally occurring plant hormones – All are acidic and abbreviated GA – Have important effects on stem elongation • Enhanced if auxin present 16
  • 16. • Adding gibberellins to certain dwarf mutants restores normal growth and development 17
  • 17. • GA is used as a signal from the embryo that turns on transcription of genes encoding hydrolytic enzymes in the aleurone layer • When GA binds to its receptor, it frees GA- dependent transcription factors from a repressor • These transcription factors can now directly affect gene expression 18
  • 18. Gibberellins • Hasten seed germination • Used commercially to extend internode length in grapes – Result is larger grapes 19
  • 19. Cytokinins • Plant hormone that, in combination with auxin, stimulates cell division and differentiation 20 Synthetic cytokinins
  • 20. • Produced in the root apical meristems and developing fruits • In all plants, cytokinins, working with other hormones, seem to regulate growth patterns • Promote the growth of lateral buds into branches • Inhibit the formation of lateral roots – Auxin promotes their formation 21
  • 21. 22
  • 22. Cytokinins • Promote the synthesis or activation of cytokinesis proteins • Also function as antiaging hormones • Agrobacterium inserts genes that increase rate of cytokinin and auxin production – Causes massive cell division – Formation of crown gall tumor 23
  • 23. • Plant tissue can form shoots, roots, or an undifferentiated mass depending on the relative amounts of auxin and cytokinin 24
  • 24. Ethylene • Gaseous hydrocarbon (H2C―CH2) • Auxin stimulates ethylene production in the tissues around the lateral bud and thus retards their growth • Ethylene also suppresses stem and root elongation • Major role in fruit development – hastens ripening – Transgenic tomato plant can’t make ethylene – Shipped without ripening and rotting 25
  • 25. Abscisic Acid • Synthesized mainly in mature green leaves, fruits, and root caps • Little evidence that this hormone plays a role in abscission • Induces formation of dormant winter buds • Counteracts gibberellins by suppressing bud growth and elongation • Counteracts auxin by promoting senescence 26
  • 26. Abscisic Acid • Necessary for dormancy in seeds – Prevents precocious germination called vivipary • Important in the opening and closing of stomata 27
  • 27. 28
  • 28. Other known hormones:- • Other identified plant growth regulators include- a)BRASSINOSTEROID:- • First discovered in the pollen of brassica spp. • Are structurally simillar to steroid hormones.
  • 29.
  • 30. Brassinosteroids • Functional overlap with other plant hormones, especially auxins and gibberellins • Broad spectrum of physiological effects – Elongation, cell division, stem bending, vascular tissue development, delayed senescence, membrane polarization and reproductive development 31
  • 31. Salicylic acid:- • Activate genes in some plants that produce chemicals that aid in the defense against pathogenic invader. Jasmonates :- • Are produced from fatty acids & seems to promote the production of defense proteins that are used to fend off invading organisms. • Also have a role in seed germination.
  • 32. plant peptide hormones:-  Involved in cell to cell signaling.  Roles in plant growth &development , including defense mechanism. Polyamines:-  Are strongly basic molecule with low molecular weight that have been found in all organism studied thus far. Nitric oxide:-  Serves as signals in hormonal &defense response.  E.G-nitrogen fixation , stomata closure , germination , cell death.
  • 33. Karrikins:- • Not plant hormones because they are not made by plants, but are a group of plant growth regulator found in the smoke of burning plant materials that have the ability to stimulates the germination of seeds. Strigolactones :-  Implicated in the inhibition of shoot branching. Triacontanol :-  A fatty alcohol that acts as a growth stimulant, especially initiating new basal breaks in the rose family.  It is found in ALFALFA (Lucerne),BEE’S WAX.
  • 34. POTENTIAL MEDICAL APPLICATION:-  Plant stress hormones activate cellular response, including cell death to diverse stress situation in plants.  Researchers have found that some plant stress hormone share the ability to adversely affect human cancer cells.  E.G:-sodium salicylate has been found to suppress proliferation of LYMPHOBLASTIC LEUKEMIA,PROSTATE &MELANOMA human cancer cells.  Methyl jasmonate has been found to induce cell death in a number of cancer cell lines.