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Photosynthesis is the process of synthesizing
carbohydrates from carbon dioxide and water with the
generation of oxygen in light.
Thus, during photosynthesis solar energy is converted into
chemical energy.
Photosynthesis = Light reaction + Dark reaction
Photosynthesis
Light reaction Dark reaction
Synthesis of assimilatory power Synthesis of sugars
(ATP + NADPH)
Z- Scheme ( Non-cyclic photo C3 Cycle/C4 Cycle/CAM
Phosphorylation) Pathway
Takes place in grana thylakoids Takes place in stroma of the
membrane chloroplast
Requires Light No light required
Primary reaction Secondary reaction
Action spectrum depicts the magnitude of response of a
biological system in response to light, e.g. Action spectrum of
photosynthesis can be made from measuring oxygen evolution
at different wavelengths.
An absorption spectrum is one which displays the amount of
light which is absorbed by a molecule or substance as a
function of wavelength.
*Chlorophyll is green in colour because it absorbs light mainly
in red and blue parts of spectrum, so only light enriched in
green wavelengths is reflected.
Quantum yield : It is defined as number of photosynthesis
products per total number of quanta absorbed.
Ø = No. of photochemical products
Total no. of quanta absorbed
Enhancement effect was discovered by Emerson. He
measured the rate of photosynthesis by using two different
wavelengths.
He found that when red and far-red wavelengths are used
together, the rate of photosynthesis is greater than sum of
individuals rates.
*Based on these observations, it was discovered that
photosynthesis is carried out by two light absorbing
pigments ( Photosystem I and Photosystem II) which
work in series. PS I absorbs maximally at far red region
(700nm) while PS II absorbs at red wavelengths (680 nm).
PS I PS II
Present in stroma thylakoids and non
appressed parts of thylakoids
Present in appressed parts of thylakoids
Reaction centre is P700 Reaction centre is P680
Active in both red and far red light Active in far red light (>680)
Carry out reduction of NADP+ to
NADPH + H+
Picks up electron emitted during
photolysis of water and performs non-
cyclic photophosphorylation
PS I pigments: Chl a-660, Chl a-670,
Chl a-680, Chl a-690, Chl a-700,
carotenoids.
LHC I, some electron carriers are
present
It’s a strong reluctant and weak oxidant
PS II pigments: Chl a-660, Chl a-670,
Chl a-680, phycobilins.
LHC II, some electrons carriers and
oxygen evolving complex are present
It’s a weak reluctant and strong oxidant
Pigments
Antenna Systems: 20-30 bacteriochlorophylls per centre –
Bacteria
200-300 chlorophylls per reaction centre- Higher plants.
Few thousands chlorophylls per reaction centre- Algae(
some)
Physical mechanism by which excitation energy is
conveyed from chlorophyll that absorbs light to reaction
center is called resonance transfer.
These antenna complexes are also called chlorophyll a/b
antenna proteins.
Photo-inhibition: It occurs when excess excitation arriving
at PS II reaction center leads to inactivation and damage.
It’s a complex set of molecular processes, defined as
inhibition of photosynthesis by excess light.
Prolong inhibition PS II reaction center must be
disassembled and repaired main target is D1 protein only.
When D1 is damaged by excess light it must be removed
from membrane and replaced with a newly synthesized
molecule.
Xanthophylls:3xanthophylls,violaxanthin,antheraxanthin
& zeaxanthin
Non-photochemical Quenching is quenching of
chlorophyll fluorescence by process other than
photochemistry. It protects photosynthetic machinery
against over-excitation and subsequent damage.
The overall quantum yield of photosynthesis is nearly
independent of wavelength strongly suggests that a
mechanisms exits that shifts energy from one PS to other
in response to different conditions.

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Photosynthesis in plants

  • 1.
  • 2. Photosynthesis is the process of synthesizing carbohydrates from carbon dioxide and water with the generation of oxygen in light. Thus, during photosynthesis solar energy is converted into chemical energy. Photosynthesis = Light reaction + Dark reaction
  • 3. Photosynthesis Light reaction Dark reaction Synthesis of assimilatory power Synthesis of sugars (ATP + NADPH) Z- Scheme ( Non-cyclic photo C3 Cycle/C4 Cycle/CAM Phosphorylation) Pathway Takes place in grana thylakoids Takes place in stroma of the membrane chloroplast Requires Light No light required Primary reaction Secondary reaction
  • 4. Action spectrum depicts the magnitude of response of a biological system in response to light, e.g. Action spectrum of photosynthesis can be made from measuring oxygen evolution at different wavelengths. An absorption spectrum is one which displays the amount of light which is absorbed by a molecule or substance as a function of wavelength. *Chlorophyll is green in colour because it absorbs light mainly in red and blue parts of spectrum, so only light enriched in green wavelengths is reflected. Quantum yield : It is defined as number of photosynthesis products per total number of quanta absorbed. Ø = No. of photochemical products Total no. of quanta absorbed
  • 5. Enhancement effect was discovered by Emerson. He measured the rate of photosynthesis by using two different wavelengths. He found that when red and far-red wavelengths are used together, the rate of photosynthesis is greater than sum of individuals rates. *Based on these observations, it was discovered that photosynthesis is carried out by two light absorbing pigments ( Photosystem I and Photosystem II) which work in series. PS I absorbs maximally at far red region (700nm) while PS II absorbs at red wavelengths (680 nm).
  • 6. PS I PS II Present in stroma thylakoids and non appressed parts of thylakoids Present in appressed parts of thylakoids Reaction centre is P700 Reaction centre is P680 Active in both red and far red light Active in far red light (>680) Carry out reduction of NADP+ to NADPH + H+ Picks up electron emitted during photolysis of water and performs non- cyclic photophosphorylation PS I pigments: Chl a-660, Chl a-670, Chl a-680, Chl a-690, Chl a-700, carotenoids. LHC I, some electron carriers are present It’s a strong reluctant and weak oxidant PS II pigments: Chl a-660, Chl a-670, Chl a-680, phycobilins. LHC II, some electrons carriers and oxygen evolving complex are present It’s a weak reluctant and strong oxidant
  • 7. Pigments Antenna Systems: 20-30 bacteriochlorophylls per centre – Bacteria 200-300 chlorophylls per reaction centre- Higher plants. Few thousands chlorophylls per reaction centre- Algae( some) Physical mechanism by which excitation energy is conveyed from chlorophyll that absorbs light to reaction center is called resonance transfer. These antenna complexes are also called chlorophyll a/b antenna proteins. Photo-inhibition: It occurs when excess excitation arriving at PS II reaction center leads to inactivation and damage. It’s a complex set of molecular processes, defined as inhibition of photosynthesis by excess light.
  • 8. Prolong inhibition PS II reaction center must be disassembled and repaired main target is D1 protein only. When D1 is damaged by excess light it must be removed from membrane and replaced with a newly synthesized molecule. Xanthophylls:3xanthophylls,violaxanthin,antheraxanthin & zeaxanthin Non-photochemical Quenching is quenching of chlorophyll fluorescence by process other than photochemistry. It protects photosynthetic machinery against over-excitation and subsequent damage. The overall quantum yield of photosynthesis is nearly independent of wavelength strongly suggests that a mechanisms exits that shifts energy from one PS to other in response to different conditions.