This document provides an overview of phage therapy. It discusses bacteriophages and their structure. It covers the growing problem of antibiotic resistance and how phage therapy could provide an alternative treatment. The history of phage therapy is reviewed, along with initial problems, solutions, administration examples, and future implications. Both advantages and disadvantages of phage therapy are presented. The document concludes that modern innovations in phage therapy combined with careful scientific methodology may help enhance its effectiveness as an alternative to antibiotics for drug-resistant bacterial infections.
Bacteriophages are viruses that infect and replicate within bacteria. They have been proposed as an alternative to antibiotics to treat drug-resistant bacterial infections. Bacteriophages are highly specific to particular bacterial strains and exhibit either lytic or lysogenic life cycles. Intact phage therapy uses whole phages, while therapies based on phage components use purified enzymes like lysins. Lysins precisely target and break down bacterial cell walls, killing the bacteria. Bacteriophage therapy and lysin therapy show promise for treating various bacterial infections but require further development and standardization.
The document discusses bacteriophages (phages), including their taxonomy, characteristics, uses in treating antibiotic resistance, and applications. Phages are viruses that infect bacteria and there are over 100 phage species. Phage therapy uses phages to treat bacterial infections and was commonly used in the early 20th century before being replaced by antibiotics. Recent interest has grown in phage therapy and other applications of phages due to increasing antibiotic resistance. Phages and their enzymes (enzybiotics) show potential for treating various infections and in industries like food processing.
phage therapy is the use of bacteriophages to kill pathogenic bacterial cells. Bacteriophages are bacterial parasites that invade bacterial cells and engulf them like blue whale fish kills euphausiids and copepodsand in sea .
Phage therapy (PT) is also called bacteriophage therapy. It uses viruses to treat bacterial infections. Bacterial viruses are called phages or bacteriophages. They only attack bacteria; phages are harmless to people, animals, and plants. Bacteriophages are the natural enemies of bacteria.
Bacteriophage therapy for antimicrobial resistant and biofilm forming [Autosa...kamal shrestha
This document discusses bacteriophage therapy as a potential treatment for antibiotic-resistant and biofilm-forming bacteria. It provides background on antibiotic resistance and biofilms, how they form and confer resistance. Bacteriophages are introduced as viruses that infect and replicate within bacteria. The history of bacteriophage therapy is covered, along with its advantages over antibiotics in being non-toxic and specifically targeting bacteria. Recent advances aim to improve efficacy, such as using cocktails of phages with broader host ranges or genetically modifying phages. Overall, the document argues that bacteriophage therapy shows promise as an alternative to antibiotics for resistant bacterial infections.
This document discusses bacteriophage therapy as an alternative approach to antibiotic resistance. It begins with an introduction to antibiotic resistance and discusses the mechanisms and factors contributing to resistance. It then introduces bacteriophage or phages, describing their classification, life cycles, and mechanisms of infecting bacteria. The document outlines methods for preparing and administering phage therapy. It discusses advantages of phage therapy over antibiotics and provides examples of phage therapy applications in food and agriculture. Finally, it addresses some challenges to phage therapy including host range, bacterial debris in preparations, and lysogeny.
Bacteriophages are viruses that infect and replicate within bacteria. They have been proposed as an alternative to antibiotics to treat drug-resistant bacterial infections. Bacteriophages are highly specific to particular bacterial strains and exhibit either lytic or lysogenic life cycles. Intact phage therapy uses whole phages, while therapies based on phage components use purified enzymes like lysins. Lysins precisely target and break down bacterial cell walls, killing the bacteria. Bacteriophage therapy and lysin therapy show promise for treating various bacterial infections but require further development and standardization.
The document discusses bacteriophages (phages), including their taxonomy, characteristics, uses in treating antibiotic resistance, and applications. Phages are viruses that infect bacteria and there are over 100 phage species. Phage therapy uses phages to treat bacterial infections and was commonly used in the early 20th century before being replaced by antibiotics. Recent interest has grown in phage therapy and other applications of phages due to increasing antibiotic resistance. Phages and their enzymes (enzybiotics) show potential for treating various infections and in industries like food processing.
phage therapy is the use of bacteriophages to kill pathogenic bacterial cells. Bacteriophages are bacterial parasites that invade bacterial cells and engulf them like blue whale fish kills euphausiids and copepodsand in sea .
Phage therapy (PT) is also called bacteriophage therapy. It uses viruses to treat bacterial infections. Bacterial viruses are called phages or bacteriophages. They only attack bacteria; phages are harmless to people, animals, and plants. Bacteriophages are the natural enemies of bacteria.
Bacteriophage therapy for antimicrobial resistant and biofilm forming [Autosa...kamal shrestha
This document discusses bacteriophage therapy as a potential treatment for antibiotic-resistant and biofilm-forming bacteria. It provides background on antibiotic resistance and biofilms, how they form and confer resistance. Bacteriophages are introduced as viruses that infect and replicate within bacteria. The history of bacteriophage therapy is covered, along with its advantages over antibiotics in being non-toxic and specifically targeting bacteria. Recent advances aim to improve efficacy, such as using cocktails of phages with broader host ranges or genetically modifying phages. Overall, the document argues that bacteriophage therapy shows promise as an alternative to antibiotics for resistant bacterial infections.
This document discusses bacteriophage therapy as an alternative approach to antibiotic resistance. It begins with an introduction to antibiotic resistance and discusses the mechanisms and factors contributing to resistance. It then introduces bacteriophage or phages, describing their classification, life cycles, and mechanisms of infecting bacteria. The document outlines methods for preparing and administering phage therapy. It discusses advantages of phage therapy over antibiotics and provides examples of phage therapy applications in food and agriculture. Finally, it addresses some challenges to phage therapy including host range, bacterial debris in preparations, and lysogeny.
Phage therapy offers a promising alternative to antibiotics by utilizing bacteriophages, or viruses that infect bacteria, to combat multidrug-resistant bacteria. Bacteriophages are highly specific to their bacterial hosts and can evolve rapidly to adapt to bacterial resistance. Phage therapy has been used successfully to treat infections caused by antibiotic-resistant bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. However, challenges remain around the regulatory approval process and identifying specific phages for each bacterial strain.
This document discusses the history and current state of bacteriophage therapy. It begins by outlining the discovery of bacteriophages in the late 19th/early 20th century. It then describes how bacteriophage therapy was used commercially in the 1940s but declined with the rise of antibiotics. Recent antibiotic resistance has revived interest in bacteriophage therapy as an alternative approach. The document outlines the structure and life cycle of bacteriophages, and describes two main approaches to bacteriophage therapy - using intact phages or purified phage components like lysins. It discusses some potential applications and challenges to therapeutic use of bacteriophages.
Phage therapy involves using bacteriophages to treat bacterial infections. Bacteriophages are viruses that infect and destroy bacteria. There are two main types of bacteriophages - lytic phages, which quickly reproduce within and destroy their host bacteria, and lysogenic phages, which integrate their DNA into the host bacteria and do not immediately kill them. Phage therapy was discovered in the early 20th century and was widely used in the former Soviet Union, though it is still being tested for use elsewhere. The advantages of phage therapy include specificity to bacteria, natural replication, and low risk of resistance development. Challenges include potential immune reactions after intravenous use and the need to culture phages that target the
Bacteriophage therapy of infections diseases.Dmitri Popov
This document discusses the use of bacteriophages to treat various bacterial infections caused by E. coli, Salmonella, Shigella, Staphylococcus, and Streptococcus. It provides information on the classification and pathogenic characteristics of these bacteria. Bacteriophages target specific bacteria and can be used as alternatives to antibiotics to treat infections and prevent the spread of disease. The document focuses on using bacteriophages therapeutically and for prophylaxis against various foodborne illnesses and infections.
bacterial secretion system, tpyes of different secretion system, type 3 secretion system , regulation, effectors role of effectors, host bactrial relation molecular activities of effectors, chaperons, atpase
This document discusses efflux pumps in bacteria. It covers several topics:
1) Efflux pumps are membrane proteins that actively export toxins and antimicrobials from bacteria. Their overexpression is a mechanism of antimicrobial resistance.
2) Efflux pumps are classified into several families based on their structure and energy source, including ABC, MATE, MFS, SMR, and RND families.
3) Efflux pumps have natural physiological functions like exporting bile acids and fatty acids, but can also export a broad range of antimicrobials, contributing to multidrug resistance.
Bacteriophages offer an alternative to antibiotics for treating bacterial infections. Bacteriophages are viruses that infect and replicate within bacteria. They have a lytic cycle where they ultimately kill the bacteria or a lysogenic cycle where they integrate into the bacterial genome. Bacteriophages were first discovered in the early 1900s and were used to treat bacterial infections before widespread antibiotic use. They target specific bacteria and can potentially adapt to overcome bacterial resistance. Producing bacteriophages at scale for therapeutic use requires growing bacteria, infecting them with phages, separating and purifying the phages. More research is still needed but bacteriophages show promise as a precision treatment for bacterial infections in the face of growing
Developing vaccines against infectious and epidemic diseases with the aid of Bioinformatics is now possible, by predicting epitopes on an antigen and finding possible targets for the antibody to bind. A new era of vaccine production is just ahead of us.
Watch out the ppt to know more!!!
History of the Forgotten Cure - Phage therapyStudent
1) The document provides a history of phage therapy, from its discovery in the late 19th/early 20th century to its decline with the rise of antibiotics and recent rediscovery due to antibiotic resistance. Key figures who discovered and explored phages and phage therapy include Ernest Hankin, Frederick Twort, and Félix d'Herelle.
2) It discusses how phage therapy works, involving using bacteriophages to treat bacterial infections. Advantages over antibiotics include phages' ability to self-replicate and adapt versus antibiotics being fixed molecules.
3) Problems that initially limited phage therapy are addressed, such as host range, bacterial debris in preparations, and lysogeny.
A DNA vaccine is a type of vaccine that transfects a specific antigen-coding DNA sequence into the cells of an organism as a mechanism to induce an immune response.
DNA vaccines work by injecting genetically engineered plasmid containing the DNA sequence encoding the antigen(s) against which an immune response is sought, so the cells directly produce the antigen, thus causing a protective immunological response.
Bacteriophage typing involves identifying viruses that infect bacteria up to the strain level. It is used to control infections and for epidemiological purposes. Strains are differentiated based on phenotypic and genotypic differences, known as 'typing'. Various typing methods exist, including phenotypic methods like biotyping, phage typing, serotyping, and genotypic methods like plasmid profiling, ribotyping, and pulse field gel electrophoresis. Each method has advantages and limitations in terms of typeability, reproducibility, discriminatory power, and ease of use. Molecular typing techniques are increasingly used for outbreak detection and epidemiological investigations.
This document discusses the history and potential applications of bacteriophage therapy. It begins by introducing bacteriophages and their ability to lyse bacteria. It then provides examples of different types of phages and describes their life cycles. The document discusses the discovery of phages in the early 20th century and their use in the former Soviet Union to treat bacterial infections. It notes that antibiotic resistance has renewed interest in phage therapy as an alternative approach. The advantages and disadvantages of phage therapy over antibiotics are presented. The document concludes by stating that further studies are needed but that phages show promise as tailored treatments for multidrug-resistant bacteria.
The document summarizes the life cycles of M13 and Mu viruses. M13 is a filamentous bacteriophage that infects E. coli. It has a single stranded DNA genome packed inside a protein capsid. It replicates through the attachment to and penetration of host cells, followed by replication of its genome and production of new virus particles. Mu is a temperate bacteriophage that can enter a lysogenic cycle by integrating its double stranded DNA into the host genome. It has an icosahedral head and tail structure and replicates through a "cut and paste" mechanism where its DNA integrates into the host chromosome.
Pathogenic mechanisms of microbes of medical importanceJoyce Mwatonoka
The document summarizes the pathogenic mechanisms of microbes that are medically important. It discusses key terms and outlines various mechanisms including adherence, invasion, evasion of host defenses, and toxigenesis. Specifically, it describes how bacteria adhere to host cells using adhesins and receptors. It also explains how they invade tissues using invasins like hyaluronidase and collagenase. Bacteria can evade host defenses by inhibiting phagocytosis and surviving inside phagocytes. Some vary antigens to avoid immune responses. Toxins including exotoxins and endotoxins are also discussed.
This document discusses bacteriophages (phages), viruses that infect bacteria. It covers the composition and structure of phages, how they infect host cells through adsorption and nucleic acid injection, and their multiplication cycles of either the lytic or lysogenic pathways. The document also discusses phage typing, which uses specific phages to identify and differentiate bacterial pathogens, and applications of phages in areas like diagnostics, therapeutics, biocontrol, and more.
The document discusses integrons and the MU phage. Integrons are genetic elements in bacteria that can integrate and express gene cassettes, allowing for the spread of antibiotic resistance. The MU phage infects enterobacteria and has a life cycle involving both lysogenic and lytic replication. It has a dsDNA genome that integrates into the host chromosome and can replicate through transposition. The phage attaches to host cells, injects its genome, and can either enter a latent phase or begin lytic replication, assembling new phage particles that are released to infect more cells.
The document summarizes antifungal chemotherapy. It discusses the different types of antifungal therapy and the various classes of antifungal agents, including azoles, allylamines, polyenes, pyrimidines, benzofurans, and lipopeptides. It describes the biochemical targets of these agents as inhibition of ergosterol synthesis, squalene epoxidase, membrane sterols, thymidylate synthetase, nucleic acid synthesis, and glucan synthesis. The goal of antifungal therapy is to selectively target differences between fungal and mammalian cells, such as fungal cell membrane ergosterol content and cell wall composition.
1. Microorganisms are classified based on their relationship with humans as commensals, pathogens, parasites, and opportunistic pathogens.
2. Pathogens have the ability to cause disease, known as pathogenicity, while virulence refers to the degree of pathogenicity.
3. Microbes can be transmitted via various routes including ingestion, inhalation, direct contact, sexual contact, bloodborne transmission, vectors, and vertical transmission.
4. For an infection to occur, a pathogen must first adhere to and invade host tissues, then survive the host's immune response through various mechanisms.
The document discusses the normal microbial flora that inhabit healthy humans. It is divided into resident and transient flora. The resident flora consists of microorganisms regularly found in a given area, while the transient flora inhabits areas temporarily. The four major phyla that make up most of the human microbiota are Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. The normal flora varies across body sites like skin, mouth, respiratory and GI tracts. Maintaining the balance of the normal flora is important for health.
This document discusses subunit and peptide vaccines. Subunit vaccines contain purified antigens from pathogens rather than whole pathogens. They often require adjuvants and multiple doses to provide long-lasting immunity. Peptide vaccines use short amino acid sequences from pathogens to stimulate immune responses. While they are stable and inexpensive to produce, peptides may not stimulate T-cells on their own and require carriers or adjuvants. The document outlines advantages and disadvantages of both subunit and peptide vaccines.
The document discusses antibiotics, their development and success, as well as the rise of antibiotic resistance. It notes that Alexander Fleming discovered penicillin in 1928, but others were able to mass produce it starting in the 1940s. While antibiotics were initially very effective, overuse and misuse has led to increasing antibiotic resistance in bacteria. The document outlines factors that promote resistance, such as antibiotic use in agriculture and overprescription, and stresses the need for monitoring resistance through programs like WHONET to track resistance trends and guide appropriate antibiotic use.
Plantibody in human and animal healthy by GOUTAMGoutam Kumar
Plantibodies are antibodies produced through genetically modified plants. They function the same as antibodies from animals. The document discusses how plantibodies are produced through transgenic plants and purified. Potential applications include using plantibodies to treat dental caries, herpes, and protect plants from pathogens. While still being researched, plantibodies show promise as a cheap, efficient way to produce therapeutic antibodies on a large scale.
Phage therapy offers a promising alternative to antibiotics by utilizing bacteriophages, or viruses that infect bacteria, to combat multidrug-resistant bacteria. Bacteriophages are highly specific to their bacterial hosts and can evolve rapidly to adapt to bacterial resistance. Phage therapy has been used successfully to treat infections caused by antibiotic-resistant bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. However, challenges remain around the regulatory approval process and identifying specific phages for each bacterial strain.
This document discusses the history and current state of bacteriophage therapy. It begins by outlining the discovery of bacteriophages in the late 19th/early 20th century. It then describes how bacteriophage therapy was used commercially in the 1940s but declined with the rise of antibiotics. Recent antibiotic resistance has revived interest in bacteriophage therapy as an alternative approach. The document outlines the structure and life cycle of bacteriophages, and describes two main approaches to bacteriophage therapy - using intact phages or purified phage components like lysins. It discusses some potential applications and challenges to therapeutic use of bacteriophages.
Phage therapy involves using bacteriophages to treat bacterial infections. Bacteriophages are viruses that infect and destroy bacteria. There are two main types of bacteriophages - lytic phages, which quickly reproduce within and destroy their host bacteria, and lysogenic phages, which integrate their DNA into the host bacteria and do not immediately kill them. Phage therapy was discovered in the early 20th century and was widely used in the former Soviet Union, though it is still being tested for use elsewhere. The advantages of phage therapy include specificity to bacteria, natural replication, and low risk of resistance development. Challenges include potential immune reactions after intravenous use and the need to culture phages that target the
Bacteriophage therapy of infections diseases.Dmitri Popov
This document discusses the use of bacteriophages to treat various bacterial infections caused by E. coli, Salmonella, Shigella, Staphylococcus, and Streptococcus. It provides information on the classification and pathogenic characteristics of these bacteria. Bacteriophages target specific bacteria and can be used as alternatives to antibiotics to treat infections and prevent the spread of disease. The document focuses on using bacteriophages therapeutically and for prophylaxis against various foodborne illnesses and infections.
bacterial secretion system, tpyes of different secretion system, type 3 secretion system , regulation, effectors role of effectors, host bactrial relation molecular activities of effectors, chaperons, atpase
This document discusses efflux pumps in bacteria. It covers several topics:
1) Efflux pumps are membrane proteins that actively export toxins and antimicrobials from bacteria. Their overexpression is a mechanism of antimicrobial resistance.
2) Efflux pumps are classified into several families based on their structure and energy source, including ABC, MATE, MFS, SMR, and RND families.
3) Efflux pumps have natural physiological functions like exporting bile acids and fatty acids, but can also export a broad range of antimicrobials, contributing to multidrug resistance.
Bacteriophages offer an alternative to antibiotics for treating bacterial infections. Bacteriophages are viruses that infect and replicate within bacteria. They have a lytic cycle where they ultimately kill the bacteria or a lysogenic cycle where they integrate into the bacterial genome. Bacteriophages were first discovered in the early 1900s and were used to treat bacterial infections before widespread antibiotic use. They target specific bacteria and can potentially adapt to overcome bacterial resistance. Producing bacteriophages at scale for therapeutic use requires growing bacteria, infecting them with phages, separating and purifying the phages. More research is still needed but bacteriophages show promise as a precision treatment for bacterial infections in the face of growing
Developing vaccines against infectious and epidemic diseases with the aid of Bioinformatics is now possible, by predicting epitopes on an antigen and finding possible targets for the antibody to bind. A new era of vaccine production is just ahead of us.
Watch out the ppt to know more!!!
History of the Forgotten Cure - Phage therapyStudent
1) The document provides a history of phage therapy, from its discovery in the late 19th/early 20th century to its decline with the rise of antibiotics and recent rediscovery due to antibiotic resistance. Key figures who discovered and explored phages and phage therapy include Ernest Hankin, Frederick Twort, and Félix d'Herelle.
2) It discusses how phage therapy works, involving using bacteriophages to treat bacterial infections. Advantages over antibiotics include phages' ability to self-replicate and adapt versus antibiotics being fixed molecules.
3) Problems that initially limited phage therapy are addressed, such as host range, bacterial debris in preparations, and lysogeny.
A DNA vaccine is a type of vaccine that transfects a specific antigen-coding DNA sequence into the cells of an organism as a mechanism to induce an immune response.
DNA vaccines work by injecting genetically engineered plasmid containing the DNA sequence encoding the antigen(s) against which an immune response is sought, so the cells directly produce the antigen, thus causing a protective immunological response.
Bacteriophage typing involves identifying viruses that infect bacteria up to the strain level. It is used to control infections and for epidemiological purposes. Strains are differentiated based on phenotypic and genotypic differences, known as 'typing'. Various typing methods exist, including phenotypic methods like biotyping, phage typing, serotyping, and genotypic methods like plasmid profiling, ribotyping, and pulse field gel electrophoresis. Each method has advantages and limitations in terms of typeability, reproducibility, discriminatory power, and ease of use. Molecular typing techniques are increasingly used for outbreak detection and epidemiological investigations.
This document discusses the history and potential applications of bacteriophage therapy. It begins by introducing bacteriophages and their ability to lyse bacteria. It then provides examples of different types of phages and describes their life cycles. The document discusses the discovery of phages in the early 20th century and their use in the former Soviet Union to treat bacterial infections. It notes that antibiotic resistance has renewed interest in phage therapy as an alternative approach. The advantages and disadvantages of phage therapy over antibiotics are presented. The document concludes by stating that further studies are needed but that phages show promise as tailored treatments for multidrug-resistant bacteria.
The document summarizes the life cycles of M13 and Mu viruses. M13 is a filamentous bacteriophage that infects E. coli. It has a single stranded DNA genome packed inside a protein capsid. It replicates through the attachment to and penetration of host cells, followed by replication of its genome and production of new virus particles. Mu is a temperate bacteriophage that can enter a lysogenic cycle by integrating its double stranded DNA into the host genome. It has an icosahedral head and tail structure and replicates through a "cut and paste" mechanism where its DNA integrates into the host chromosome.
Pathogenic mechanisms of microbes of medical importanceJoyce Mwatonoka
The document summarizes the pathogenic mechanisms of microbes that are medically important. It discusses key terms and outlines various mechanisms including adherence, invasion, evasion of host defenses, and toxigenesis. Specifically, it describes how bacteria adhere to host cells using adhesins and receptors. It also explains how they invade tissues using invasins like hyaluronidase and collagenase. Bacteria can evade host defenses by inhibiting phagocytosis and surviving inside phagocytes. Some vary antigens to avoid immune responses. Toxins including exotoxins and endotoxins are also discussed.
This document discusses bacteriophages (phages), viruses that infect bacteria. It covers the composition and structure of phages, how they infect host cells through adsorption and nucleic acid injection, and their multiplication cycles of either the lytic or lysogenic pathways. The document also discusses phage typing, which uses specific phages to identify and differentiate bacterial pathogens, and applications of phages in areas like diagnostics, therapeutics, biocontrol, and more.
The document discusses integrons and the MU phage. Integrons are genetic elements in bacteria that can integrate and express gene cassettes, allowing for the spread of antibiotic resistance. The MU phage infects enterobacteria and has a life cycle involving both lysogenic and lytic replication. It has a dsDNA genome that integrates into the host chromosome and can replicate through transposition. The phage attaches to host cells, injects its genome, and can either enter a latent phase or begin lytic replication, assembling new phage particles that are released to infect more cells.
The document summarizes antifungal chemotherapy. It discusses the different types of antifungal therapy and the various classes of antifungal agents, including azoles, allylamines, polyenes, pyrimidines, benzofurans, and lipopeptides. It describes the biochemical targets of these agents as inhibition of ergosterol synthesis, squalene epoxidase, membrane sterols, thymidylate synthetase, nucleic acid synthesis, and glucan synthesis. The goal of antifungal therapy is to selectively target differences between fungal and mammalian cells, such as fungal cell membrane ergosterol content and cell wall composition.
1. Microorganisms are classified based on their relationship with humans as commensals, pathogens, parasites, and opportunistic pathogens.
2. Pathogens have the ability to cause disease, known as pathogenicity, while virulence refers to the degree of pathogenicity.
3. Microbes can be transmitted via various routes including ingestion, inhalation, direct contact, sexual contact, bloodborne transmission, vectors, and vertical transmission.
4. For an infection to occur, a pathogen must first adhere to and invade host tissues, then survive the host's immune response through various mechanisms.
The document discusses the normal microbial flora that inhabit healthy humans. It is divided into resident and transient flora. The resident flora consists of microorganisms regularly found in a given area, while the transient flora inhabits areas temporarily. The four major phyla that make up most of the human microbiota are Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. The normal flora varies across body sites like skin, mouth, respiratory and GI tracts. Maintaining the balance of the normal flora is important for health.
This document discusses subunit and peptide vaccines. Subunit vaccines contain purified antigens from pathogens rather than whole pathogens. They often require adjuvants and multiple doses to provide long-lasting immunity. Peptide vaccines use short amino acid sequences from pathogens to stimulate immune responses. While they are stable and inexpensive to produce, peptides may not stimulate T-cells on their own and require carriers or adjuvants. The document outlines advantages and disadvantages of both subunit and peptide vaccines.
The document discusses antibiotics, their development and success, as well as the rise of antibiotic resistance. It notes that Alexander Fleming discovered penicillin in 1928, but others were able to mass produce it starting in the 1940s. While antibiotics were initially very effective, overuse and misuse has led to increasing antibiotic resistance in bacteria. The document outlines factors that promote resistance, such as antibiotic use in agriculture and overprescription, and stresses the need for monitoring resistance through programs like WHONET to track resistance trends and guide appropriate antibiotic use.
Plantibody in human and animal healthy by GOUTAMGoutam Kumar
Plantibodies are antibodies produced through genetically modified plants. They function the same as antibodies from animals. The document discusses how plantibodies are produced through transgenic plants and purified. Potential applications include using plantibodies to treat dental caries, herpes, and protect plants from pathogens. While still being researched, plantibodies show promise as a cheap, efficient way to produce therapeutic antibodies on a large scale.
Recombinant vaccines use genetic engineering techniques to produce antigens that induce protective immunity. They offer advantages over conventional vaccines like improved safety and defined composition. Recombinant vaccines work by inserting genes for antigens into vectors like viruses. This allows the vector to produce the antigen and elicit an immune response. They can target specific cells and induce immunity through multiple routes of administration. While live recombinant vaccines carry a risk of reversion, they elicit strong immune responses from just one or a few doses. Future areas of development include improved delivery methods and use of immunomodulators and plant expression systems.
This document discusses different types of traditional vaccines, including inactivated, live attenuated, toxoid, and subunit vaccines. It provides details on how each type is prepared, such as using heat or chemicals to kill pathogens for inactivated vaccines and weakening live viruses for live attenuated vaccines. Both advantages and disadvantages are described for each approach. Specific examples like the polio, diphtheria, tetanus, and influenza vaccines are examined in more depth. The history of vaccine development from Lady Mary Wortley Montagu's variolation technique to Louis Pasteur's first live attenuated vaccine for chicken cholera is also reviewed.
This document presents information about vaccines. It begins with a brief introduction defining a vaccine as a biological preparation that provides immunity against a disease. The history section then discusses how Edward Jenner developed the smallpox vaccine in 1796 and how Louis Pasteur later developed vaccines for chicken cholera and anthrax in the 1880s. The document goes on to describe the main types of vaccines as live attenuated, killed/inactivated, toxoid, subunit, conjugate, DNA, and recombinant vector vaccines. It also provides examples of diseases each vaccine type is used for and discusses their advantages and disadvantages.
This document provides information about edible vaccines. It begins by defining an ideal vaccine and then defines vaccines in general. It introduces the concept of edible vaccines, which are genetically engineered foods that express vaccine antigens to provide immunity. The document discusses the mechanisms of action, methods for transforming DNA into plants, candidate plant species, examples, factors affecting efficacy, applications, limitations, regulatory issues, recent discoveries, advantages and disadvantages, and future aspects of edible vaccines.
This document discusses antimicrobial resistance, including its definition, causes, mechanisms, and impact. It notes that resistance arises naturally but is accelerated by misuse and overuse of antimicrobials in both humans and animals. Resistance occurs via several mechanisms including inactivating drugs, modifying drug targets in bacteria, exporting drugs from bacteria, reducing drug permeability, and mutating genes involved in drug mechanisms of action. It highlights several important antibiotic-resistant bacteria like MRSA, VRE, and multi-drug resistant TB. The global spread of resistance poses a major threat to modern medicine.
Antimicrobial resistance occurs when microbes evolve a mechanism that protects them from the effects of antimicrobials or antibiotics. Antibiotic resistance is a subset of antimicrobial resistance, as it applies to bacteria that become resistant to antibiotics. Resistant microbes are more difficult to treat, requiring higher doses, or alternative medications that may prove more toxic.
1. Vaccination in fish involves exposing them to non-pathogenic forms of microorganisms to stimulate protective immune responses. This process is referred to as vaccination.
2. When feasible, effective and safe, vaccination is one of the most cost-effective measures for controlling infectious disease in fish. Common fish vaccines include killed whole cell vaccines, live-attenuated vaccines, and recombinant DNA vaccines.
3. Vaccine delivery methods include injection, immersion, and oral delivery using foods like Artemia. The duration of protection depends on the vaccine and may require booster doses. Vaccination is an important tool for disease control in commercial aquaculture.
Recombinant DNA technology allows for the creation of recombinant viral vector vaccines. These vaccines use an attenuated viral vector to deliver foreign genes from a pathogen and induce an immune response against the pathogen. Recombinant vaccines have advantages over conventional vaccines like defined composition, safety since they use non-pathogenic vectors, and the potential for a single vaccine against multiple diseases. However, challenges remain in ensuring the viral vectors do not regain virulence and in the immune response against the viral delivery system. Future areas of development include improved vaccine delivery methods and the use of immunomodulators to enhance vaccine efficacy.
This document discusses antimicrobial resistance and provides definitions, history, and mechanisms. It defines antimicrobial resistance as the ability of microorganisms like bacteria, viruses, and parasites to stop antimicrobial drugs from working against them. The discovery of antimicrobials created new treatments but microbes developed resistance over time. Factors that contribute to resistance include overuse of antibiotics, lack of sanitation, and transmission of resistant genes between bacteria. Resistance occurs via natural and acquired mechanisms, the latter being a major clinical problem. Strategies to address resistance include prudent antibiotic use, developing new drugs, and alternative approaches like phage therapy.
This document provides information about the Virology/Parasitology course code 320 at semester 6. It discusses the lytic and lysogenic cycles of bacteriophages, including their adsorption, penetration, synthesis of phage components, maturation and assembly, and release stages. It also covers the significance of bacteriophages in phage typing, assays, conferring new properties to host bacteria, and applications in medicine, agriculture and wastewater treatment. Additionally, it introduces viroids as small circular RNA molecules that cause plant diseases, and prions as misfolded protein particles that cause transmissible spongiform encephalopathies in humans and animals.
No doubt that antibiotics are the life saver for us but taking them without prescription of doctor or not completing its course can turn them against us ,more precisely it makes the bacteria more powerful and hard to cure. They are not affected with antibiotic anymore this is known as Antibiotic Resistance
This document provides information about vaccines and immunotechnology. It begins with an introduction to vaccines, defining them as preparations used to prevent disease by inducing an immune response. It then discusses the key properties of good vaccines and different types of vaccines including live, killed, subunit, and recombinant vaccines. The document specifically focuses on recombinant vaccines, noting their advantages over conventional vaccines and discussing viral vectors used to deliver genes encoding antigens. It concludes by discussing future developments and providing references.
Vaccines work by exposing the immune system to antigens from pathogens to induce an immune response. Major types include whole organism vaccines using live attenuated or killed pathogens, as well as purified components like toxoids, polysaccharides, and recombinant antigens. Current COVID-19 vaccines use viral vectors or genetic material to produce antigens and trigger protective immunity. Vaccines have saved millions of lives through immunization programs by conferring protection against viral and bacterial diseases.
Microbes come in two main types - prokaryotes and eukaryotes. Prokaryotes like bacteria have no nucleus and reproduce quickly through binary fission. Eukaryotes like yeast are single-celled fungi used for producing antibiotics and in biotechnology. Microbes and their enzymes are useful as tools in cloning, protein expression, and reporting systems. Microorganisms are also used to produce foods and therapeutics through fermentation, and to develop vaccines, diagnostics, and countermeasures against bioweapons.
This document discusses bacteriophages, which are viruses that infect bacteria. It describes the two main cycles of bacteriophage multiplication: the lytic cycle and lysogenic cycle. The lytic cycle involves the T-even bacteriophage infecting E. coli, reproducing through five stages, and ultimately causing the host cell to lyse. The lysogenic cycle is exemplified by the lambda bacteriophage infecting E. coli, which can either undergo the lytic cycle or integrate its DNA into the host and remain dormant. Bacteriophages can be grown and quantified using plaque assays. They have various applications including phage therapy and transduction.
Recent advances in vaccine development
The document discusses recent advances in vaccine development technologies, including DNA vaccines, transgenic plant vaccines, sugar glass vaccines, skin patch vaccines, and combination vaccines. DNA vaccines work by delivering pathogen genes into the body to produce antigens and elicit an immune response. Transgenic plant vaccines produce antigens in edible plants that are eaten to deliver the vaccine. Sugar glass vaccines preserve vaccine potency by immobilizing antigens in a sugar glass matrix. Skin patch vaccines target skin immune cells for vaccination. Combination vaccines provide protection against multiple diseases in a single vaccine dose. The document also discusses challenges in vaccine development like inadequate preclinical data, lack of information on target populations, high development costs, and antigenic variation requiring constant vaccine
Co-Chairs, Val J. Lowe, MD, and Cyrus A. Raji, MD, PhD, prepared useful Practice Aids pertaining to Alzheimer’s disease for this CME/AAPA activity titled “Alzheimer’s Disease Case Conference: Gearing Up for the Expanding Role of Neuroradiology in Diagnosis and Treatment.” For the full presentation, downloadable Practice Aids, and complete CME/AAPA information, and to apply for credit, please visit us at https://bit.ly/3PvVY25. CME/AAPA credit will be available until June 28, 2025.
Breast cancer: Post menopausal endocrine therapyDr. Sumit KUMAR
Breast cancer in postmenopausal women with hormone receptor-positive (HR+) status is a common and complex condition that necessitates a multifaceted approach to management. HR+ breast cancer means that the cancer cells grow in response to hormones such as estrogen and progesterone. This subtype is prevalent among postmenopausal women and typically exhibits a more indolent course compared to other forms of breast cancer, which allows for a variety of treatment options.
Diagnosis and Staging
The diagnosis of HR+ breast cancer begins with clinical evaluation, imaging, and biopsy. Imaging modalities such as mammography, ultrasound, and MRI help in assessing the extent of the disease. Histopathological examination and immunohistochemical staining of the biopsy sample confirm the diagnosis and hormone receptor status by identifying the presence of estrogen receptors (ER) and progesterone receptors (PR) on the tumor cells.
Staging involves determining the size of the tumor (T), the involvement of regional lymph nodes (N), and the presence of distant metastasis (M). The American Joint Committee on Cancer (AJCC) staging system is commonly used. Accurate staging is critical as it guides treatment decisions.
Treatment Options
Endocrine Therapy
Endocrine therapy is the cornerstone of treatment for HR+ breast cancer in postmenopausal women. The primary goal is to reduce the levels of estrogen or block its effects on cancer cells. Commonly used agents include:
Selective Estrogen Receptor Modulators (SERMs): Tamoxifen is a SERM that binds to estrogen receptors, blocking estrogen from stimulating breast cancer cells. It is effective but may have side effects such as increased risk of endometrial cancer and thromboembolic events.
Aromatase Inhibitors (AIs): These drugs, including anastrozole, letrozole, and exemestane, lower estrogen levels by inhibiting the aromatase enzyme, which converts androgens to estrogen in peripheral tissues. AIs are generally preferred in postmenopausal women due to their efficacy and safety profile compared to tamoxifen.
Selective Estrogen Receptor Downregulators (SERDs): Fulvestrant is a SERD that degrades estrogen receptors and is used in cases where resistance to other endocrine therapies develops.
Combination Therapies
Combining endocrine therapy with other treatments enhances efficacy. Examples include:
Endocrine Therapy with CDK4/6 Inhibitors: Palbociclib, ribociclib, and abemaciclib are CDK4/6 inhibitors that, when combined with endocrine therapy, significantly improve progression-free survival in advanced HR+ breast cancer.
Endocrine Therapy with mTOR Inhibitors: Everolimus, an mTOR inhibitor, can be added to endocrine therapy for patients who have developed resistance to aromatase inhibitors.
Chemotherapy
Chemotherapy is generally reserved for patients with high-risk features, such as large tumor size, high-grade histology, or extensive lymph node involvement. Regimens often include anthracyclines and taxanes.
low birth weight presentation. Low birth weight (LBW) infant is defined as the one whose birth weight is less than 2500g irrespective of their gestational age. Premature birth and low birth weight(LBW) is still a serious problem in newborn. Causing high morbidity and mortality rate worldwide. The nursing care provide to low birth weight babies is crucial in promoting their overall health and development. Through careful assessment, diagnosis,, planning, and evaluation plays a vital role in ensuring these vulnerable infants receive the specialize care they need. In India every third of the infant weight less than 2500g.
Birth period, socioeconomical status, nutritional and intrauterine environment are the factors influencing low birth weight
The skin is the largest organ and its health plays a vital role among the other sense organs. The skin concerns like acne breakout, psoriasis, or anything similar along the lines, finding a qualified and experienced dermatologist becomes paramount.
Osteoporosis - Definition , Evaluation and Management .pdfJim Jacob Roy
Osteoporosis is an increasing cause of morbidity among the elderly.
In this document , a brief outline of osteoporosis is given , including the risk factors of osteoporosis fractures , the indications for testing bone mineral density and the management of osteoporosis
- Video recording of this lecture in English language: https://youtu.be/Pt1nA32sdHQ
- Video recording of this lecture in Arabic language: https://youtu.be/uFdc9F0rlP0
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Nano-gold for Cancer Therapy chemistry investigatory projectSIVAVINAYAKPK
chemistry investigatory project
The development of nanogold-based cancer therapy could revolutionize oncology by providing a more targeted, less invasive treatment option. This project contributes to the growing body of research aimed at harnessing nanotechnology for medical applications, paving the way for future clinical trials and potential commercial applications.
Cancer remains one of the leading causes of death worldwide, prompting the need for innovative treatment methods. Nanotechnology offers promising new approaches, including the use of gold nanoparticles (nanogold) for targeted cancer therapy. Nanogold particles possess unique physical and chemical properties that make them suitable for drug delivery, imaging, and photothermal therapy.
8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptxHolistified Wellness
We’re talking about Vedic Meditation, a form of meditation that has been around for at least 5,000 years. Back then, the people who lived in the Indus Valley, now known as India and Pakistan, practised meditation as a fundamental part of daily life. This knowledge that has given us yoga and Ayurveda, was known as Veda, hence the name Vedic. And though there are some written records, the practice has been passed down verbally from generation to generation.
Summer is a time for fun in the sun, but the heat and humidity can also wreak havoc on your skin. From itchy rashes to unwanted pigmentation, several skin conditions become more prevalent during these warmer months.
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotesPsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
2. Contents
• Phages-An Introduction
• Phage structure
• Fates of phages
• Antibiotic resistance-growing problem
• Phage therapy
• History
• Why phage therapy??
• Initial problems
• Solutions
• Prerequisites for phage therapy
• Administration and examples
• Future implications
• Advantages and disadvantages
• Challenges
• Conclusions
3. Bacteriophage (Phage)
• Definition - Obligate intracellular parasites that multiply inside bacteria by making use
of some or all of the host biosynthetic machinery.
• Significance
– Phage therapy
– Gene transfer in bacteria
– Phage display
– Medical applications
• Identification of bacteria - phage typing
• Treatment and prophylaxsis???
4. Structure of bacteriophage
• A bacteriophage particle consists of a single stranded DNA or RNA molecule,
encapsulated inside a protein coat or lipoprotein coat.
• Tail morphologies: long , flexible tails, double layered, contractile tails.
• The size of phage head is correlated to the size of genome being packaged and varies in
diameter between 45 and 100nm.
8. Antibiotic resistance-growing
problem
• Main reason is the abusive use of antibiotics over past twenty years.
• The resistance phenomenon represents not only an important healthcare issue but also an
economic problem.
• Penicillin fails to completely eradicate Streptococci in 35% of the pateints.
• Infections caused by Streptomyces agalactiae in pregnant women cannot be treated with
antibiotics because of the risk of abortion.
9. Phage therapy
• Phage therapy is the therapeutic use of bacteriophages for the treatment of pathogenic
bacterial infections.
• This method of therapy is still being tested for treatment of a variety of bacterial
infections.
• Has not yet been approved in countries other than Georgia.
• Phage therapy has many potential applications in human medicines as well as dentistry ,
veterinary science and agriculture.
• Bacteriophages are much more specific (host specific) and have a high therapeutic
index.
10. History
Edward Twort (1915) and Felix d'Herelle (1917) independently reported isolating
filterable entities capable of destroying bacterial cultures and of producing small cleared
areas on bacterial lawns.
It was F d'Herelle, a Canadian working at the Pasteur Institute in Paris, who gave them
the name "bacteriophages"-- using the suffix phage (1922).
Frederick Twort
1915 Félix d'Hérelle 1917
13. Bacteriophages Antibiotics Comments
Very specific (i.e., usually affect only the targeted
bacterial species); therefore, chances of
developing secondary infections are avoided
Antibiotics target both pathogenic microorganisms
and normal microflora. This affects the microbial
balance in the patient, which may lead to serious
secondary infections.
High specificity may be considered to be a
disadvantage of phages because the disease-
causing bacterium must be identified before phage
therapy can be successfully initiated. Antibiotics
have a higher probability of being effective than
phages when the identity of the etiologic agent has
not been determined.
Replicate at the site of infection and are thus
available where they are most needed
They are metabolized and eliminated from the
body and do not necessarily concentrate at the site
of infection.
The "exponential growth" of phages at the site of
infection may require less frequent phage
administration in order to achieve the optimal
therapeutic effect.
No serious side effects have been described. Multiple side effects, including intestinal
disorders, allergies, and secondary infections (e.g.,
yeast infections) have been reported
A few minor side effects reported for therapeutic
phages may have been due to the liberation of
endotoxins from bacteria lysed in vivo by the
phages. Such effects also may be observed when
antibiotics are used
Phage-resistant bacteria remain susceptible to
other phages having a similar target range.
Resistance to antibiotics is not limited to targeted
bacteria.
Because of their more broad-spectrum activity,
antibiotics select for many resistant bacterial
species, not just for resistant mutants of the
targeted bacteria
Selecting new phages (e.g., against phage-resistant
bacteria) is a relatively rapid process that can
frequently be accomplished in days or weeks.
Developing a new antibiotic (e.g., against
antibiotic-resistant bacteria) is a time-consuming
process and may take several years
Evolutionary arguments support the idea that
active phages can be selected against every
antibiotic-resistant or phage-resistant bacterium
by the ever-ongoing process of natural selection.
14. Initial problems
• Problem # 1: Specific host range
• Problem # 2: Bacterial debris present in phage preparations.
• Problem # 3: Attempts to remove host bacteria from therapeutic preparations
• Problem # 4: Rapid clearance of phages
• Problem # 5: Lysogeny
• Problem # 6: Lack of knowledge
16. Solutions
• Use of phage mixtures (cocktails)
• Application in chronic infections: time to select appropriate phages
• Broad spectrum phages (e.g. all S. aureus) exist.
• Add phages to antibiotics
• Study of genome of phage
17. Prerequisites for phage therapy
Various prerequisites that should be met:
1. Phage therapy should not be attempted before the biology of the therapeutic phage is
well understood.
2. Phage preparations should meet all safety requirements.
3. Phage preparations should contain infective phage particles.
4. The phage receptor should be known.
5. The efficacy of phage should be tested in an animal model.
18. Culture-commercial preparations
• D’Herelle’s commercial laboratory in Paris produced at least 5 different phage
preparation against various bacterial infection.
• The preparations were called as:
• Bacte-coli-phage
• Bacte-rhino-phage
• Bacte-intesti-phage
• Bacte-pyo-phage
• Bacte-staphy-phage.
• Therapeutic phages were also produced in United States.
• In the 1940’s Eli Lilly Company produced seven phage products for human use.
19. Administration
• Orally
• Topically on infected wounds
• Application in liquid form is possible and stored preferably in refrigerated vials
• Injections are rarely used
20. Examples
• Killing of Mycobacterium avium and Mycobacterium tuberculosis by a
Mycobacteriophage delivered by a non virulent Mycobacterium
• Tuberculosis is a serious health problem that results in millions of deaths around the
world each year
• Mycobacterium smegmatis , an avirulent mycobacterium is used to deliver the lytic
phage TM4 where both M.avium and M.tuberculosis reside within macrophages
• These results showed that treatment of M.avium infected as well as M.tuberculosis
infected with M.smegmatis infected with TM4 resulted in significant reduction in
number of viable intracellular bacillli.
21. Example # 2
• Killing of S.aureus by using bacteriophage that kills S.aureus .
• By treating the infection with the use of phage impregnated pad
• Successful results were reported
23. Future implications
• As a vaccine delivery vehicle
• Prophylaxsis???
• Phage display
• Phage typing
• Genetically manipulated lysogenic phages for in situ gene delivery:
--> in situ delivery to bacterial cells of
* killing genes (doc)
* antisense RNA to block translation
Westwater et al. 2003. Use of a genetically engineered phage to deliver antimicrobial agents
to bacteria: an alternative therapy for treatment of bacterial infections.
Phages as bio-control and bacteriophage bioprocessing
24. As vaccine delivery vehicle
• The stability of whole bacteriophage lambda particles, used as a DNA vaccine delivery
system has been examined.
• When phage lambda was diluted into water, a marginal loss in titre was observed over a
2-week period.
• Over a 24 h period, liquid phage suspensions were stable within the pH range pH 3-11,
therefore oral administration of bacteriophage DNA vaccines via drinking water may be
possible.
25. Phage typing
• Phage typing is also known as the use of sensitivity patterns to specific phages for
precisely identifying microbial strains.
• The sensitivity of detection would be increased if the phages bound to bacteria are
detected by specific antibodies
• The technique has most extensively been used for the detection of Mycobacterium
tuberculosis , E.coli , Pseudomonas , Salmonella , Listeria and Campylobacter species
26. Advantages and disadvantages
Advantages
• Phages are very specific and do not
harm the useful bacteria that live in and
in the body
• They replicate at the site of infection
• They are active against antibiotic
resistant bacteria
• Once administered it will not need more
dosages
Disadvantages
• The great specificity is also a
disadvantage when the exact species of
bacteria is unknown and also in case of
multiple infections
• Infections whose agents are hidden in
the interior of the cells may be
inaccessible to phages
27. Cont’d
Advantages
• High expectations of safety
• Can carry accessory genes for additional
therapeutic benefits
Disadvantages
• Resistance can arise (may use
cocktails)
• Immune response of host may limit
or prevent the re-use
• The development of phage–
neutralizing antibodies-The
production of neutralizing
antibodies should not be a significant
obstacle during initial or relatively
short-term therapeutic treatments at
least.
28. Challenges
• Specificity of phages
• Novelty
• Efficacy and other technical challenges
• Regulatory approvals
• Market acceptance
• Patient safety
29. Conclusions
• Multidrug resistance bacteria have opened a second window for phage therapy
• Modern innovations combined with careful scientific methodology can enhance
mankind’s ability to make it work this time around
• Phage therapy can then serve as a stand alone therapy for infections that are fully
resistant
• It will then be serve as a co-therapeutic agent for infections that are still susceptible to
antibiotics by helping to prevent the emergence of bacterial mutants against either agent
30.
31.
32. References
• Hugos and Russel’s Microbiology
• Carlton R M (1999) Phage therapy: Past history and Future prospects
• Broxmeyer et al. 2002. J. Infect. Dis. 186:1155-1160.
• (Dabrowska et al. 2005. Bacteriophage penetration in vertebrates. J. Appl.
Microbiol. 98: 7-13.)
• Antimicrob. Agents Chemother. 47: 1301-1307.
• Nature Reviews/Drug Discovery 2: 489-497.
• www.phagetherapycenter.com/ - Phage Therapy Center of Tbilsi, Georgia.
“…effective treatment solution for patients who have bacterial infections that do
not respond to conventional antibiotics”
• http://www.researchgate.net/publication/8514842_Bacteriophage_lambda_is_a_h
ighly_stable_DNA_vaccine_delivery_vehicle
Editor's Notes
Plaque assay
Method
Plaque forming unit (pfu)
Measures infectious particles
in situ means to examine the phenomenon exactly in place where it occurs (i.e., without moving it to some special medium).
Phage display is a laboratory technique for the study of protein–protein, protein–peptide, and protein–DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them.[1] In this technique, a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to "display" the protein on its outside while containing the gene for the protein on its inside, resulting in a connection between genotype and phenotype. These displaying phages can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those other molecules. In this way, large libraries of proteins can be screened and amplified in a process called in vitro selection, which is analogous to natural selection.
Review Article
Bacteriophages for prophylaxis and therapy in cattle, poultry and pigs
Abstract
The successful use of virulent (lytic) bacteriophages (phages) in preventing and treating neonatal enterotoxigenic Escherichia coli infections in calves, lambs and pigs has prompted investigation of other applications of phage therapy in food animals. While results have been very variable, some indicate that phage therapy is potentially useful in virulent Salmonella and E. coli infections in chickens, calves and pigs, and in control of the food-borne pathogens Salmonella and Campylobacter jejuni in chickens and E. coli O157:H7 in cattle. However, more rigorous and comprehensive research is required to determine the true potential of phage therapy. Particular challenges include the selection and characterization of phages, practical modes of administration, and development of formulations that maintain the viability of phages for administration. Also, meaningful evaluation of phage therapy will require animal studies that closely represent the intended use, and will include thorough investigation of the emergence and characteristics of phage resistant bacteria. As well, effective use will require understanding the ecology and dynamics of the endemic and therapeutic phages and their interactions with target bacteria in the farm environment. In the event that the potential of phage therapy is realized, adoption will depend on its efficacy and complementarity relative to other interventions. Another potential challenge will be regulatory approval.
(Received October 15 2008)
(Accepted October 19 2008)
Phage typing is a method used for detecting single strains of bacteria. It is used to trace the source of outbreaks of infections.[1] The viruses that infect bacteria are called bacteriophages ("phages" for short) and some of these can only infect a single strain of bacteria. These phages are used to identify different strains of bacteria within a single species. A culture of the strain is grown in the agar and dried. A grid is drawn on the base of the petri dish to mark out different regions. Inoculation of each square of the grid is done by a different phage. The phage drops are allowed to dry and are incubated: The susceptible phage regions will show a circular clearing where the bacteria have been lysed, and this is used in differentiation