This document discusses various biological markers of aging, including theories of aging like the free radical theory. It outlines how hormones like testosterone, estrogen, progesterone, cortisol, DHEA, growth hormone, and melatonin decline with age and affect physiological functions. Graphs show levels of these hormones by age and over the menstrual cycle. Abnormal levels of cortisol, DHEA, and melatonin are associated with various health issues. Circadian rhythms and factors influencing melatonin are outlined. Calorie restriction and related compounds that mimic its effects on markers like AMPK, sirtuins, and mTOR are also discussed.