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OS20 - Assessment of potency and effectiveness hepta-valent FMD vaccine oil adjuvanted (ISA 206) in Egypt - Abdel-Hamid Bazid
1. 1EuFMD | Open Session special edition | #OS20se
Abdel-Hamid Bazid (presenter)
MEVAC / University of Sadat City / Egypt
ASSESSMENT OF POTENCY AND EFFECTIVENESS OF
HEPTA-VALENT FMD VACCINE OIL ADJUVANTED (ISA 206)
IN EGYPT
2. 2EuFMD | Open Session special edition | #OS20se
1- Introduction
Foot and mouth disease (FMD) is a terrible highly
contagious viral disease affecting cloven hoofed
domestic and wild animals, leading to severe economic
impacts. Vaccination is the most effective strategy for
controlling the infection.
2- Aim of the Study
In the present study, Potency and effectiveness of a
Hepta-valent FMD oil-based FMD vaccine formulated
with Montanide ISA 206 VG W/O/W emulsion adjuvant,
with the following virus strains/serotypes (A-Iran05, A-
Africa-IV, O-PanAsia2, O-Manisa, O-EA3, SAT-2 Garbia
and SAT-2 LIB-12)were evaluated under experimental
and field conditions. The evaluated parameters are
1- safety
2- potency and efficacy
3- field effectiveness
3- Material and methods
1- FMD virus strains
2- Virus inactivation and concentration
3- Antigen preparation and vaccine formulation:
4- Safety and sterility test
5- Challenge test:
6- Serum neutralizing antibody assay
3. 3EuFMD | Open Session special edition | #OS20se
Table (1)In vivo safety of prepared FMD vaccine
Table (2)The challenge test for vaccinated cattle with
A-Africa (GIV), O-EA3 and SAT-2 (Lib12)
Figure (1) Mean FMD serum neutralization antibody titers in
vaccinated cattle with hepta-valent vaccine
4. 4
In conclusion,
1. the results of this study showed that a single administration of ISA 206 VG oil-based adjuvant
Heptavalent vaccine induced high mean neutralizing antibody titers at 28 days post vaccination as
well as it induced complete protection in vaccinated animals after challenge by 104 MLD50
homologous FMD strain.
2. Under field condition the Heptavalent vaccine provided superior clinical protection after two doses
(prime and poster doses) and the protection extended for 4 months according to the presented results
3. The results suggest that the vaccine formulated with ISA 206 VG (W/O/W) emulsion oil-based
adjuvant can be a good and effective vaccine in enzootic countries such as Egypt.